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Fluorescence Microscopy

Leica Microsystems Fluorescence Microscopy - See the Light!

Fluorescence applications have gained importance in a lot of different fields in biomedical research. There is hardly any application field nowadays where fluorescence does not play a role for the identification, classification and particularly for quantitative measurements of biological structures and processes.

Tutorial Fluorescence Microscopy

This tutorial explains the optical elements in the light path and the operating mode of fluorescence microscopy taking the example of an inverted and motorized high-end research light microscope which can be used for transmitted light contrasting methods and fluorescence microscopy.

Absorbing needs, emitting solutions

The efficiency of exciting a fluorescent dye is mainly dependent on the selection of the right fluorescence filter sets or fluorescence filtercubes. With Leica Microsystems FluoScout™, our web-based application for comparing and selecting a number of fluorescence filter cubes and fluorophores, this can be done very easily.

Learn more about the FluoScout™

Leica FluoScout™: Adding fluorophores

Leica Microsystems FluoScout™: Find the best filter cube for your needs

Absorbing science, emitting innovation

Not only the world’s first epifluorescence microscope in 1932, but also the use of dichroic mirrors in fluorescence filter cubes were major developments for improving the efficiency and image quality of fluorescence microscopes. Wild Leitz, the predecessor of Leica Microsystems, invented the Ploemopak® illuminator (1967), the world’s first epifluorescence axis using fluorescence filter cubes like the ones used in every fluorescence microscope today.

Besides these innovations Leica Microsystems has set other milestones:

  • 2005 – First in offering one integrated software platform(LAS AF) for both confocal and widefield systems
  • 2011 – First and still the only company offering super-resolution systems in confocal and widefield microscopy

Modern fluorescence microscopes offer a variety of automated functions for simplifying the setup of experiments. Learn more about the optical components of a fluorescence microscope  and the principles of modern epifluorescence microscopy in our step-by-step tutorial on Leica Science Lab.

Absorbing complexity, emitting simplicity

Straightforward setup and control of experiments is becoming more and more important In fluorescence microscopy. The dedicated Leica Application Suite Advanced Fluorescence (LAS AF) software platform facilitates the setup of even complex experiments. LAS AF guides you from the setup to the analysis of the imaging data. From easy multi-channel experiments to complex 3D analysis, LAS AF is the perfect software platform.

Widefield Experiment Setup in LAS AF. LAS AF guides you from basic single- channel image acquisition to complex multidimensional experiments. The clear separation of “setup area” and “viewer area” enables relaxed working without losing control of your experiment.

Absorbing data, emitting beauty

To acquire fluorescence images, it is necessary to use the appropriate fluorescence camera. Leica Microsystems offers dedicated fluorescence cameras for all application needs. Each camera provides the perfect match of sensitivity and image quality to ensure superb results.

Cultured neuronal cells. Merged fluorescent multichannel image made out of four fluorescent channels sequentially acquired using a Leica DFC3000 G digital camera. Blue: nuclei, green: astrocytes, red: mature neurons, purple: immature neurons. Sample courtesy of FAN GmbH, Martinsried, Germany.

Fluorescence Filter Sets

Leica stereo fluorescence filter sets fulfill the highest quality standards and guarantee excellent research results. For every imaginable application in the life science research field, we can offer the matching excitation and emission filter set. Have a look into the Leica fluorescence filter set database.

Light Microscopes for Fluorescence

Stereo Microscopes for Fluorescence

Microscope Cameras for Fluorescence

Intraoperative Fluorescence