The Fast Track to Super-Resolution Leica TCS STED CW
The new Leica TCS STED CW confocal microscope is the most straightforward way to nanoscopy for research. The system reveals sub-cellular details below 80 nm – based on a purely optical approach. Sub-diffraction multicolor images provide new insights into cellular architecture.
The depletion laser and the excitation lines in the visible range enable researchers to use conventional dyes such as Alexa 488, FITC and Oregon Green and established fluorescent proteins such as YFP.
Dynamics of sub-cellular processes can be imaged due to this flexibility and the system's ability of fast data acquisition. This means: live cell imaging below the diffraction limit!
Your Advantages
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Freedom of choice - Popular Fluorescent Dyes and ProteinsSTED microscopy in the visible range enables to use labels like Alexa 488 and Oregon Green– plus genetically encoded standard markers like YFP for sub-diffraction imaging. Watch live cell experiments at the nanoscale! |
Observe What's Inside - with Confocal Super-ResolutionThe superior optical sectioning of the true point scanning system Leica TCS SP5 provides super-resolution deep inside the sample. Acquire STED-images inside a 12 µm thick drosophila larvae featuring a thick cuticula, record complete 3D stacks. Find out what this can do for your research. |
Quick and Affordable Upgrade to STEDThe modular STED concept allows installed Leica TCS SP5 systems to be upgraded on-site and in a short time. The STED upgrade can be tailored to your needs. |
Nanoscale Imaging - Devoid of Mathematical ArtefactsProfit from super-resolution pixel by pixel independent of recording speed or dye. STED is a purely optical super-resolution concept. The extreme robustness against interframe-drifts protects your data and makes data handling easy and safe. |
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“This is the end of colocalization analysis as we know it”(Prof. P. McPherson, Montreal Neurological Institute of McGill University) Seeing how two structures are organized in relation to one another in sub-diffraction detail opens the door to a completely new world of colocalization studies. The Leica TCS STED CW gives immediate access to dualcolor super-resolution images. By choosing dyes that differ in their excitation spectra, but are similar in emission, it has become possible to use only one depletion laser instead of two separate light sources. The advantage: reduced complexity and exclusion of any potential artifacts because of chromatic aberrations. And no need for mathematical corrections. |
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Images Leica TCS STED CW
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