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Leica DM1000 Life Science Research

Uniquely Ergonomic System Microscope Leica DM1000

Lluis Torner, ICFO Director and Christoph Thumser, Sales Director for Life Science Research EMEA celebrating the new agreement with members of their teams.

The Institute of Photonic Science, Barcelona becomes a new NANOSCOPY IMAGING REFERENCE SITE for Leica Microsystems

Barcelona, October 9th 2017. October 6th marked the beginning of a new partnership between ICFO - The Institute of Photonic Sciences in Barcelona and Leica Microsystems. The new collaboration agreement aiming to promote and establish ICFO as a new European Nanoscopy Imaging Reference Site for Leica. As such, ICFO’s experts in super-resolution will partner with Leica Microsystems to conceptualize and implement technological improvements to the state-of-the-art systems from Leica.

This new collaboration sees the installation of the latest generation in nanoscopy systems from Leica Microsystems at the ICFO – the Leica microscopy system TCS SP8 STED 3X. Through the collaboration, Leica Microsystems and ICFO will work together to further develop the technology behind nanoscopy, find new applications in biomedicine and biophysics, and to offer access to nanoscopy to a wide community of researchers across Europe, including members of the Euro-BioImaging, Corbel European and LaserLab research infrastructure programs.

Nanoscopy overcomes earlier limits of optical microscopy by resolving structures down to just a few nanometers through methods such as STED. Nanoscopy is a key investigative tool in the most important medical research of today, including research into neurodegenerative diseases such as Parkinson’s and Alzheimers. In Barcelona, researchers already use Leica Microsystems nanoscopy systems for example, in cooperation with the Bellvitge Hospital and the University of Barcelona to  study retinal degeneration, and the Sant Pau Hospital in Barcelona study beta-amyloid plaques and their effects on Alzheimer’s disease.

This three-year R&D collaboration expects to bring fruitful results within the European biomedical research community. As Pablo Loza, ICFO researcher and leader of the SLN facility at ICFO comments, “We are thrilled with the addition of this new Leica state-of-the-art microscope and the new collaboration agreement set forward with Leica Microsystems R&D team. We are sure the new  TCS SP8 STED 3X, combined with all other instruments within our SLN facility at ICFO, will produce a qualitative jump on today’s research and applications and will enable us to delve into a new realm of novel biomedical applications that we are yet to fathom”.

Christoph Thumser, Sales Director for Life Science Research EMEA stated “Leica Microsystems are delighted to embark on this collaboration with the ICFO. We are confident that the combination of ICFO’s expertise in photonics and optical technology and Leica Microsystems strengths in advanced imaging solutions will lead to great developments in microscopy. The ICFO’s strong links to the imaging community through its association with CORBEL and Eurobioimaging will also benefit  Leica Microsystems.  Due to the SLN’s plan to offer open access to the Leica microscopy system TCS SP8 STED 3X, we will learn a great deal about how nanoscopy is used today and how it should develop into the future.

Tile scan image of Mouse brain tissue section with multicolor immunofluorescent labelin

The DMi8 S with LASX Navigator allows researchers to quickly create high resolution overviews like the whole mouse brain in this example. This facilitates orientation on the sample and easy discovery of critical regions for further examination. Image courtesy of Dr. Wei Mo, School of Life Sciences, Xiamen University, China.

Find the Missing Links in Advanced Live Cell Research - Leica launches DMi8 S live cell imaging solution

Wetzlar, Germany – Leica introduces the DMi8 S, a new complete solution designed to give researchers the ultimate tool for fast, versatile microscopic imaging of living cells allowing scientists to find, observe and interact with living cells like never before.

"Live-cell imaging is the future in discoveries. Gaining insight on dynamic processes is key to understand the mechanisms, potential treatments and prevention of human diseases," said Markus Lusser, President of Leica Microsystems. "These new DMi8 S developments have far reaching utility. Whether studying neuroscience, cancer, or immunology, researchers can use the DMi8 S to get elusive answers to their questions and find the links to our complex biology."

The DMi8 S imaging solution from Leica provides 5x more speed, and an increased viewing area up to 10,000x. This can be combined with the new photomanipulation scanner to activate, ablate, and bleach within one experiment. For super resolution and nanoscopy, the Infinity TIRF has been added allowing simultaneous multi-color imaging with single molecule resolution. This allows researchers to see more, see faster, and find the hidden, opening up the next chapter in widefield imaging.

The Leica DMi8 S will be launched at the 17th International European Light Microscopy Initiative Meeting (ELMI) May 23 - 26, in Dubrovnik, Croatia.

Visit www.leica-microsystems.com/dmi8 for more information on the new Leica DMi8 S.

Background

At the heart of the DMi8 S is a newly added Synapse real-time controller that enables users to get their data more quickly and gather finer time-resolved data. In addition the new LAS X Navigator is a powerful GPS style overview tool to visualize all types of samples including slides, dishes and multi well plates.

Utilizing the modular Infinity Port architecture of the DMi8 S, Leica has added advanced fluorescence capabilities with the Infinity TIRF and the Infinity Scanner. With the highly flexible Infinity TIRF module, researchers can execute simultaneous multicolor EPI, Hi-Lo and TIRF illumination as well as a high power illumination option for super resolution applications like GSD/ dSTORM and PALM.

The Infinity Scanner photomanipulation module gives users an unprecedented multimodal ability to use the latest developments in photoactivatable and photo switchable fluorescent proteins. Researchers can now interact directly with and initiate events in living cells by performing a combination of tasks like photobleaching, photoconversion, optogenetics and ablation within a single experiment.

Zebrafish heart

Beating heart of zebrafish larva (56 hours after fertilization). Myocytes are expressing GCaMP (calcium indicator) and dsRed (cell marker). Imaged in-vivo using SCAPE at 25 volumes per second. 335 x 288 x 156 micron field of view. Credit: Hillman/Li/Targoff, Columbia University

Fruit fly brain

Whole brain of adult Drosophila acquired in-vivo at 10 volumes per second using SCAPE. Neuronal subset expressing GFP (green), mushroom body neurons expressing dsRed. 450 x 264 x 227 micron field of view. Credit: Hillman/Li/Schaffer, Columbia University

Real-time 3D imaging of living organisms at sub-cellular resolution

Leica Microsystems to develop SCAPE microscopy

Mannheim/Wetzlar, Germany. Leica Microsystems CMS GmbH has entered into an exclusive, worldwide licensing agreement with Columbia University in New York to commercialize SCAPE microscopy for Life Science applications. SCAPE (swept confocally aligned planar excitation) microscopy forms 3D images of living samples by scanning them with a sheet of laser light. SCAPE’s unique capabilities allow scientists to perform fundamentally new kinds of experiments, from imaging individual neurons firing throughout the brain of adult fruit flies, to tracking calcium waves through cells in the beating heart of a zebrafish. SCAPE also stands to create new inroads for understanding diseases such as cancer, and for the development of new drugs and therapies.

SCAPE microscopy was developed in the laboratory of Elizabeth Hillman, PhD, associate professor of biomedical engineering and radiology at Columbia University and a principal investigator at Columbia’s Mortimer B. Zuckerman Mind Brain Behavior Institute. SCAPE’s ingenuity lies in being able to both scan and image a moving light sheet through a single, stationary objective lens. SCAPE delivers 3D-imaging speeds that are 10 to 100 times faster than conventional point-scanning microscopes, while maintaining the benefits of light-sheet imaging including low photodamage. Compared to conventional light-sheet microscopes that require multiple objective lenses and complex sample positioning, SCAPE’s patented single-objective approach greatly diversifies the range of intact and freely moving samples that can be imaged in 3D at near video-rates. SCAPE technology was recognized late last year with a prestigious grant award from the National Institutes of Health BRAIN Initiative.

“SCAPE’s ability to perform real-time 3D imaging at cellular resolution in living, freely moving organisms is a new frontier for neuroscience research,” said Dr. Hillman. “Beyond neuroscience, SCAPE is enabling fundamentally new scientific experiments by transforming our ability to capture 3D structure and function, movement, behavior and cellular activity in real-time across a wide range of organisms and biological samples.”

In addition to their own intellectual property, Leica Microsystems has also exclusively licensed OPM (Oblique Plane Microscopy) technology from Imperial Innovations. The technology was invented by Christopher Dunsby, PhD, Faculty of Natural Sciences, Department of Physics at Imperial College London. “Leica Microsystems is committed to investing in the most promising technologies in order to drive our innovation, and these are excellent examples of that strategy” said Markus Lusser, President of Leica Microsystems. “We recognize that the accelerating use of GCaMPs, fluorescent reporters and optogenetics presents an urgent need for high-speed volumetric imaging of living samples – and that this is a current major gap in the microscopy market. We are thrilled to have the opportunity to develop next-generation imaging systems that will drive discoveries in neuroscience, biology and medicine by capturing life in action” – said Prof. Julian F Burke, Chief Scientific Officer of Leica Microsystems. 

OM-U, the first ultramicrotome from Reichert. It was built in 1957/58.

OM-U, the first ultramicrotome from Reichert. It was built in 1957/58.

Vienna branch of Leica Microsystems celebrates 140th anniversary

Specialists in electron microscope sample preparation workflows

Vienna, Austria. Leica Mikrosysteme GmbH, the Vienna branch of Leica Microsystems, celebrates its 140th anniversary. The Vienna location hosts the electron microscopy sample preparation business of the global technology company. With this business, Leica Microsystems is the only supplier of instruments for the entire electron microscopy workflow – for biological or industrial samples under room or cryo temperature. The Vienna branch has its roots in a small opto-mechanical microscope workshop founded by Carl Reichert in 1876.

This small company secured its place in microscopy history in 1911 when Dr. Karl Reichert, one of the founder’s sons, built the first fluorescence microscope in collaboration with opto-technician Oskar Heimstädt. Cooperating with the chemical scientist Max Haitinger, he made significant progress in this field. By applying Haitinger’s method, the scientist Dr. P. Hagemann from Cologne was able to show bacteria in fluorescent light for the first time in 1938.

At C. Reichert, ultramicrotomes were a focus. These instruments are used to produce thin sections of embedded samples so that the structures of the sample can be viewed with an electron microscope. From the mid-1950s, Reichert built ultramicrotomes based on a design of the biologist Prof. Dr. Hellmuth Sitte. This successful collaboration contributed to the company’s globally leading position in this field.

The Vienna branch goes back to a small, opto-mechanical workshop in Vienna Josefstadt, which Carl Reichert, (1851-1922) founded in 1876. The company manufactured microscopes, quickly becoming successful and, after a few changes of location, finally settling in the present company building in Vienna’s 17th municipal district in 1900. Internationally, the company gained in importance, which led to the name Reichert becoming a synonym for microscopes of the highest quality. After the death of Carl Reichert, his two sons succeeded him.

In 1962, the Reichert family decided to collaborate with American Optical Corporation (AOC) based in Southbridge, USA. AOC was acquired by Warner Lambert, an American pharmaceutical company in 1968. When Warner Lambert sold all non-pharmaceutical businesses in 1985/86, Cambridge Instruments became the main shareholder from May 2, 1986. This change was the beginning of an highly successful period economically, during which the company name was changed to Reichert-Jung Optische Werke AG in June 1986 to reflect the intensified cooperation with Jung.

1989 the companies Wild Leitz Holding AG and Cambridge Instruments Company plc were joined to form Leica Microsystems, which in turn was acquired by the American technology corporation Danaher. The acquisition and integration of the company Bal-Tec into Leica Microsystems in 2008 marked the entry into the scanning electron microscopy market. The Bal-Tec portfolio complemented the existing product range for transmission microscopy so that Leica Microsystems is now the only supplier of instruments for the entire process in the electron microscopy laboratory which are used to prepare biological, medical and industrial samples. 

Microscopy Today 2016 Innovation Award to Leica TCS SP8 DLS confocal

Leica Microsystems’ Digital LightSheet solution, the Leica TCS SP8 DLS confocal microscope was honored with the Microscopy Today 2016 Innovation Award.

Pseudo-colored eye of a zebrafish embryo. Image acquired with the Leica TCS SP8 DLS. Courtesy of Dr. Basile Gurchenkov, Imaging Center of the IGBMC, Illkirch-Graffenstaden, France

Pseudo-colored eye of a zebrafish embryo. Image acquired with the Leica TCS SP8 DLS. Courtesy of Dr. Basile Gurchenkov, Imaging Center of the IGBMC, Illkirch-Graffenstaden, France

Microscopy Today 2016 Innovation Award for Leica Digital LightSheet solution

Light sheet and confocal microscopy combined for long-term imaging of sensitive samples

Mannheim, Germany. Leica Microsystems’ Digital LightSheet solution, the Leica TCS SP8 DLS confocal microscope was honored with the Microscopy Today 2016 Innovation Award in Columbus, Ohio. Its design combines the Leica TCS SP8 confocal platform with light sheet technology as a fully integrated module, thus enabling researchers to combine confocal and light sheet imaging in one experiment. Since light sheet microscopy is gentle on the sample, and increases cell viability, it is notably interesting for long-term imaging at high temporal and spatial sampling rates. Especially sensitive samples, such as embryos or 3D cell cultures, benefit from this technology. The combination moreover enables researchers to photo-manipulate their specimens using the confocal mode and apply light sheet imaging for the subsequent long-term observations. The Leica TCS SP8 DLS is one of ten achievements that Microscopy Today, the journal of the Microscopy Society of America, singles out annually for their importance and usefulness to the microscopy community.

“It is an honor to receive the Microscopy Today 2016 Innovation Award for our Digital LightSheet solution,” says Markus Lusser, President of Leica Microsystems. “The Award proves how much researchers benefit from the combination of light sheet and confocal microscopy in one system, especially since it gives them immense flexibility in the design of their experiments. Researchers especially praise the image quality and acquisition speed of the system. I am proud to say that many have been astonished at the extraordinary performance of the light sheet module on the confocal for long-term imaging of sensitive samples.”

In light sheet microscopy, the specimen is illuminated in a single plane, which reduces phototoxic effects and protects the sample. By moving the sample along this plane, imaging of three-dimensional structures is possible. A high-speed camera allows the imaging of fast cellular processes. Due to its gentleness, light sheet microscopy increases the cell viability – with the Leica TCS SP8 DLS, it can easily be scaled up to image several specimens in multi-position experiments for greater productivity.

The light sheet module is fully integrated into the Leica TCS SP8 modular imaging platform and expands its imaging options. Every inverted Leica TCS SP8 can be upgraded to a light sheet microscope and additional functionality such as STED super-resolution, multiphoton microscopy, advanced lasers or quantitative imaging can be added whenever required.

The Leica DFC9000 sCMOS microscope camera is highly sensitive with its high quantum efficiency of up to 82% and extremely low noise. The image shows a paramecium expressing GFP-Epiplasmin and Cy5-polyglycylated Tubulin. Nucleus stained with Hoechst 33258 (blue). Acquisition with LAS X. Specimen courtesy of Dr. A. Aubusson-Fleury, CGM-CNRS, France

The Leica DFC9000 sCMOS microscope camera mounted to a Leica DMi8 inverted microscope. With the combination of the microscope’s 19 mm camera port and 19 mm sensor diagonal to the camera, researchers see more and ensure they do not miss any data.

Leica sCMOS microscope camera for imaging live cells under near-native conditions

Wetzlar, Germany. Leica Microsystems launches the Leica DFC9000, a monochrome microscope camera with a highly sensitive third-generation sCMOS sensor. The camera enables researchers to image live cells under near-native conditions, allowing them to gain a better understanding of cellular processes and dynamics.

The Leica DFC9000 features an sCMOS sensor with high quantum efficiency over the entire spectrum of light, which provides a high signal-to-noise ratio to securely detect even faint signals. Compared to the second generation sensor, the maximum quantum efficiency increased by 14%, totaling up to 82% depending on wavelength as illustrated in the graphic below. In combination with a very low noise level, this results in a crisp fluorescence signal against a dark background – an effect very much desired in high-end fluorescence live cell imaging. The high sensitivity of the camera eliminates the need to monitor GFP-overexpressing specimens and protects cells from phototoxicity. The camera acquires full-frame images at a standard rate of 50 frames per second (fps) in the USB 3.0 interface version and at 90 fps with the Camera Link interface version. Higher frame rates can be achieved with partial readout of, for example, 512 x 512 pixels with 270 fps. This means researchers will not miss any fast cellular processes.

"sCMOS cameras have become the gold standard in demanding, high-end live cell imaging. Their sensitivity and speed ensure that users can capture data even from very fast processes – with other sensors these would be lost. With the Leica DFC9000, we launch the first Leica sCMOS camera. It enables researchers to get closer to reality, because they can image live cells under near-native conditions. This camera fits many of our research microscopes perfectly: Their 19 mm camera ports are designed to take full advantage of the 19 mm sensor diagonal, making sure researchers benefit from this really large field of view," says Markus Lusser, President of Leica Microsystems.

Microscopes from Leica Microsystems, like the inverted Leica DMi8 or the upright Leica DM6 B, offer a fully optically corrected 19 mm camera port that allows researchers to make full use of the sensor and achieve a real field of view of 19 mm over the entire imaging system.

The Leica DFC9000 is available with a USB 3.0 interface as the Leica DFC9000 GT, and with a Camera Link interface as the Leica DFC9000 GTC.

More information about the Leica DFC9000 SCMOS Microscope Camera.

Introduction to digital camera technology on Leica Science Lab.

With the super-resolution Leica TCS SP8 STED 3X, details of a cell can be focused down to under 50 nanometers. The image shows the pores of a cell nucleus (stained green), single protein molecules (red) and fibers of a cytoskeleton (white) in a HeLa-Cell.

The Light-Sheet-Microscope Leica TCS SP8 DLS offers the possibility to gently examine big, living samples and visualize them in 3D – the example shows the eye of a fruit fly. Specimen courtesy of Nadja Dinges, Roignant Lab, IMB Mainz.

The Multiphoton Microscope Leica TCS SP8 MP allows a particularly deep view into thick samples. This image shows brain tissue of a living mouse with microglia cells (stained green) and a blood vessel (red), taken through a thinned skull.

Image of artery endothelial cells, taken with the inverted widefield microscope Leica DMi8. The cell nucleus is stained blue, the mitochondria red and the fibers of the cytoskeleton are green.

Leica Bioimaging Center

Leica Microsystems and the Biomedical Center (BMC) in Munich open Core Facility Bioimaging for Applied Cell Research

Wetzlar, Germany. On February 17th, 2016, Leica Microsystems and the Biomedical Center (BMC) of the Ludwig-Maximilians-Universität (LMU) Munich, Germany, will inaugurate the new core facility Bioimaging. Leica Microsystems will use the facility as reference and demo center. The reference center for state-of-the-art light microscopy at the BMC is the result of a strategic co-operation between Leica Microsystems and the LMU. The Leica Bioimaging Center provides the opportunity for a close cooperation between microscope developers and users to develop innovations in modern light microscopy and establish their application in applied cell research.

For the researchers of the BMC, the Leica Bioimaging Center offers access to the latest technologies in light microscopy, enabling them to make the smallest structures of the cell visible, even at the nanoscale, and investigate biological processes on molecular level. Among these technologies are the super-resolution STED (Stimulated Emission Depletion) microscopy, which was awarded the Nobel Prize in Chemistry in 2014, and Light-Sheet Microscopy, especially suited for big, living samples. The Leica Bioimaging Center is the biggest reference and demo center to be established by Leica Microsystems in Europe, so far. Customers and potential customers can discover the latest Leica systems here, see them used in research on site, and exchange experiences with the users at the facility. In addition, new products can be tested thoroughly by the users of the core facility before being introduced to the market. The facility will be operated by the Walter-Brendel-Centre of Experimental Medicine, one of the eight resident professorships at the BMC.

"To us, the strategic partnership with the BMC – a high level, interdisciplinary research center – offers excellent possibilities to work closely together with the users of our microscopes. This will enable us to understand the requirements of our customers even better and to focus on the development of innovations which will help science to further decipher the secrets of life and win the fight against currently terminal illnesses," says Markus Lusser, President of Leica Microsystems. "We are happy to be able to work together with the researchers of the BMC on developing the Bioimaging Center into a high-level light microscopy facility for cell research."

"In many aspects, biomedical research is a very abstract field of research, as the scientists can often investigate the molecular connections only indirectly," says Professor Peter Becker, Chair of Molecular Biology and Executive Director of the Biomedical Center (BMC). "Therefore, high resolution microscopy techniques are highly important, as they make the cell structures visible and offer the opportunity to review and visualize the ideas."

"We are happy about the close cooperation with Leica Microsystems," comments Dr Steffen Dietzel, Manager of the Core Facility Bioimaging. "It gives us the chance to provide our users with microscopes which permanently meet the highest requirements in terms of configuration and maintenance."

Comprising 60 research groups, the BMC, which was opened on the HighTechCampus of the LMU in October 2015, is one of the biggest research centers in Germany to be established within the last years. As a "Center for Applied Cell Research", the BMC is closing the gap between basic research and clinical applications. At the Leica Bioimaging Center, 10 microscope systems of the latest generation are at the disposal of the researchers – among them a 3D super-resolution system Leica TCS SP8 STED 3X with integrated light sheet module, two Leica TCS SP8 MP multiphoton systems, two Leica TCS SP8 confocal microscopes and one high-end widefield microscope Leica DMi8 for live cell research.

Scientific symposium for inauguration

The inauguration of the Leica Bioimaging Center will be accompanied by a scientific symposium in the afternoon of February 17th, 2016. The topic "Plasticity of Cell Programs as Visualized by Advanced Microscopy Techniques" is relevant to scientists from various areas, such as physiology, molecular biology, immunology and cell biology. Participation at the symposium is free – however, registration is requested via:

http://www.leica-microsystems.com/inauguration-symposium/

Instantly see the true nature. Left: The ring structure of the basal bodies of cilia in Paramecium are easily resolved with HyVolution on a Leica TCS SP8, but not with traditional confocal imaging. Sample courtesy of Anne Aubusson-Fleury, CNRS, Gif-sur-Yvette, France. Right: HyVolution resolves a nanoruler such as DNA origami that has a defined 140 nm fluorophore spacing.

Instantly see the true nature with HyVolution for confocal microscopy

Leica TCS SP8 with HyVolution for greatest fidelity in multicolor confocal imaging

Mannheim, Germany. Leica Microsystems launches the HyVolution true confocal super-resolution technology for the Leica TCS SP8 confocal microscope platform, extending its imaging portfolio in the sub-diffraction regime. HyVolution allows researchers to achieve everything at the same time: They can resolve structures down to 140 nm with multiple colors, with high imaging speed, and high signal-to-noise ratio. HyVolution can be ordered with every new Leica TCS SP8 and is also available as an upgrade to existing Leica TCS SP8 platforms.

“HyVolution is high-fidelity imaging for every sample and every experiment. When studying rapid dynamics of living cells, researchers do not have to make a trade-off between high resolution, imaging speed and the number of colors they want to acquire. For the first time, these parameters are available with maximum efficiency at the same time in a confocal microscope. This opens new areas for researchers,” says Markus Lusser, President of Leica Microsystems.

“Functional annotations of the genome require a deep insight into signaling networks. This often demands sub 200 nm resolution with multiple dye emissions. HyVolution bridges the gap between classic confocal imaging and STED super-resolution below 30 nm,” explains Dr. Bernd Sägmüller, Director Confocal Laser Scanning Microscopy at Leica Microsystems. “HyVolution offers users multicolor super-resolution down to 140 nm with a confocal microscope at an affordable price.”

HyVolution is based on combinations of the unique Leica HyD hybrid detectors, a workflow-oriented software wizard, best-in-class Huygens deconvolution software embedded via a direct interface in the Leica Application Suite X (LAS X) control software and CUDA GPU-accelerated computing. Researchers benefit from HyVolution by imaging fast dynamic processes and capturing multiple colors simultaneously with the spectral detection system of the Leica TCS SP8 and still achieve 140 nm resolution.

HyVolution technology was previewed at the annual meeting of the Society for Neuroscience (SfN) 2015 in Chicago, Illinois, October 17-21. Visitors could also see the integrated light sheet microscope Leica TCS SP8 DLS, the Leica TCS SP8 STED 3X super-resolution microscope with an upright microscope stand, and the Leica TCS SP8 MP multiphoton microscope.

More information about HyVolution

An image gallery with HyVolution images

Leica Microsystems launches fully coded, semi-automated polarization microscope Leica DM4 P.

New Fully Coded, Semi-Automated Polarization Microscope

Leica DM4 P for Convenient Investigation of Crystalline Structures

Wetzlar, Germany. Leica Microsystems launches the Leica DM4 P polarization microscope for the investigation of crystalline structures such as minerals, plastics and polymers, drugs and pharmaceuticals, or pigments and cement. The Leica DM4 P is fully coded and semi-automated, can either be configured with transmitted light axis or incident light axis, as well as with both transmitted and incident light axis, which makes the instrument an ideal tool for all polarization tasks. Users benefit from LED illumination which lights samples homogeneously and at a constant color temperature at all microscope settings. Strain-free optics and objectives guarantee that birefringence stems from the samples, not from the optics. The optional equipment with coded Bertrand lens and magnification changer ensures optimal results for conoscopic observation.

Users can store and recall information due to the coded components of the Leica DM4 P. While changing objectives, illumination intensity and aperture settings are automatically recalled by the Illumination and Contrast Manager. The coded 6-fold nosepiece ensures that the aquired images are automatically calibrated. In addition, the store and recall function in the Leica Application Suite software enables reproducible image settings.

Kay Scheffler, Product Manager at Leica Microsystems, says: “The possibility to combine transmitted and incident light in the Leica DM4 P makes users highly flexible in their applications: Incident light is interesting for all those who measure reflectivity for example in ores or coal. To measure birefrengence, users need transmitted light, for example in inspection of geological thin sections, polymer foils, or drugs. Yet for specific applications such as in research, both are necessary.”

With LED illumination, users can ensure homogeneous lighting of their samples at a constant color temperature, adjusting light intensity fast. Additionally, it saves energy and does not need replacing due to its long lifetime. It emits little heat so that no cooling fan is needed. As a result, it works noiselessly.

To achieve optimal images in polarization microscopy, users need strain-free optical components of the microscope and the objectives. They guarantee that the birefringence originates from the sample, not from the optics. For conoscopy, to investigate interference figures, users need a Bertrand lens module which can be optionally added to the microscope.

More information on polarization microscopy on Science Lab

Step-by-step polarization tutorial

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Leica Microsystems launches Leica DM4 M and Leica DM6 M automated upright microscopes for materials science and materials analysis.

New Automated Upright Microscopes for Materials Science and Analysis

Leica DM4 M and Leica DM6 M for Consistent Imaging Conditions and Reliable Results

Wetzlar, Germany. Leica Microsystems launches the Leica DM4 M and Leica DM6 M upright microscopes for materials investigation. The systems are designed for imaging, measurement, and analysis of similar features across many samples and materials. While the Leica DM4 M is designed for manual routine inspection, the Leica DM6 M is capable of fully automated materials analysis. Automated functions such as the Illumination and Contrast Manager make users’ work lives easier because they reduce complex adjustments to the push of a button. Both instruments are equipped with LED illumination. For applications such as steel inclusion rating, particle analysis, phase or grain analysis, users benefit from expert modules of the Leica Application Software (LAS).

Stefan Motyka, Senior Product Manager at Leica Microsystems says: “Users can easily recall microscope settings from previous work, from any sample type. This enables them to instantly reproduce the perfect imaging parameters at any time – made possible with our Store and Recall software function. What makes these two systems unique is that they are really easy to use. One example: The microscope’s one-button Intelligent Automation simplifies any repetitive workflow. The Leica DM4 M and Leica DM6 M reduce training time, improve users’ workflows, and yield brilliant imaging results. For example, the 1.25x Panorama objective gives users a fantastic overview over the sample.”

The Illumination and Contrast Manager supports users by automatically recognizing the selected contrast technique and objective in use, accurately opening and closing the aperture and field diaphragms, and adapting the light intensity accordingly. LED illumination takes care of homogenous lighting with a constant color temperature at all intensity levels and any microscope setting. This way, the camera setting and white balance can remain constant as well and do not need adjusting. LEDs are energy-efficient, radiate little heat, and last up to 25,000 hours.

The microscope software Leica Application Suite (LAS) turns the microscope and its accessories such as a camera into an imaging system. “LAS is workflow-oriented and helps users at every step in their tasks. With the Leica DM6 M, a lot of expert modules of the LAS such as Cleanliness Expert, Grain Expert, Phase Expert, or Steel Expert are a valuable help, since they adhere to documentation regulations and enable users to produce reports in a very short time,” explains Motyka.

Questions and answer about the instruments

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