Images Leica HCS A

Rare events on-the-fly

Highly complex assay designs are possible with the LAS AF MATRIX M3 Developer Suite. The Mitocheck project*, conducted at the EMBL in Heidelberg is an excellent example for comprehensive and flexible automation.

The self-acting process automates rare event mitosis identification, combining pre-scan, object selection and high content object scan.

Microtubule secondary screen: Scrambled sRNA Tubulin (green), H2B (red). Leica TCS SP5. Objective: 63x oil (prescan); zoomed, maximum projection: 30 x 0.4 µm slices, two channels (high-resolution). Courtesy of Christian Conrad, EMBL, Heidelberg, Germany.*Mitocheck Project: www.mitocheck.org.

Easy automation - we keep it simple!

LAS AF MATRIX Mosaic

Fine details and the overview matter to evaluate experimental results.
Leica HCS A provides entirely new designed mosaic algorithms for excellent results. At the push of a button large high content images are fully automatically generated.

Mouse diaphragm muscle stained against neuro-filament 150, Mosaic: xyz: 5 x 5 x 101 images. Green: secondary antibody coupled to AlexaFluor 488 and acetylcholine receptors. Red: alpha-bungarotoxin coupled to AlexaFluor 647. Courtesy of Dr. Rüdiger Rudolf , Cellular Signalling in Skeletal Muscle, Karlsruhe Institute of Technology, Germany.

LAS AF MATRIX Multiwell

Answers to more complex questions require a larger number of sophisticated experiments. Leica HCS A supports frequently used multiwell plate formats to study multi-dimensional experiments automatically and time resolved.

Zebrafish. Danio rerio, Neurogenin - GFP. H2A
Courtesy of J. Legradi, Dr. U. Liebel, KIT Karlsruhe Institute of Technology, Germany.