Studying subcellular architecture and dynamics with conventional fluorescence microscopy can be challenging as structures smaller than 200 nm are lost. The Leica TCS SP8 STED super-resolution microscope allows you to discover more and provides fast, intuitive, purely optical access to structural details smaller than 50 nm. This means: Colocalization studies and live cell imaging – at the nanoscale!
STED microscopy is a stunningly simple way to overcome the diffraction limit - where fluorescence is generated is scaled down to sub-diffraction size. Stimulated emission switches off the ability of the dye to fluoresce in the periphery of the excitation spot yielding confocal super-resolution pixel by pixel.