Images Leica TCS STED

Physiology: muscle research with STED

Rat myofibrils stained with ATTO 647N conjugated antibodies against an N-terminal epitope in the titin molecule (clone T12), which lies at the edges of the z-disc in the sarcomere.

Top: STED. Bottom: Confocal. The scalebar indicates 1 µm.

Courtesy of Dr. Elisabeth Ehler, Kings College, London, UK

Comparison of resolution - STED vs. confocal

Colloidal crystal structure of fluorescent nanospheres labeled with ATTO 647N.

Layer of fluorescent nano-beads imaged under optimal confocal conditions (bottom) and using the same settings with the STED-depletion laser turned on (top).

The scalebar indicates 2 µm.

Courtesy of Max Planck Institute for Biophysikal Chemistry, Goettingen, Germany

Membrane domains

Syntaxin 1A Nano-domains of the basolateral membrane of PC12 cells. The nanoresolving power of STED micorscopy allows for the determination of size and denisity of these biological entities.

Sample: courtesy of Prof. Dr. Torsten Lang, University of Bonn, Germany

Immunodetection of the SYCP3 protein

Immunodetection of the SYCP3 protein on mouse spermatocytes at the
diplotene stage of meiotic prophase. Label: ATTO647N.
Bernard de Massy, Institut de Génétique Humaine, UPR1142, CNRS, Montpellier, Franc

Nuclear structures visualized with Chromeo 494 (green) and Atto 647N (red)

Sample courtesy of Dr. L. Schermelleh, LMU Biozentrum, Munich

Pre- and postsynaptic labelling with ATTO 647N and Chromeo 494

Courtesy Dr. W. Zuschratter, IfN Magdeburg.

Superresolution colocalization analysis

This example illustrates the gain in information by dual-channel STED.

While it seems in confocal that there is one green bigger structure and two red particles wich can be separated, the activation of STED reveals 3 red particles and two green. (red: ATTO647N, green: Chromeo494).