Gallery Leica TCS STED

Physiology: muscle research with STED
Rat myofibrils stained with ATTO 647N conjugated antibodies against an N-terminal epitope in the titin molecule (clone T12), which lies at the edges of the z-disc in the sarcomere.
Top: STED. Bottom: Confocal. The scalebar indicates 1 µm.
Courtesy of Dr. Elisabeth Ehler, Kings College, London, UK

Comparison of resolution - STED vs. confocal
Colloidal crystal structure of fluorescent nanospheres ATTO 647N.
Layer of fluorescent nano-beads imaged under optimal confocal conditions (bottom) and using the same settings with the STED-depletion laser turned on (top).
The scalebar indicates 2 µm.
Courtesy of Max Planck Institute for Biophysikal Chemistry, Goettingen, Germany

Membrane domains
Clustering of Syntaxin 1A Nano-domains of the basolateral membrane of PC12 cells. The nanoresolving power of STED micorscopy allows for the determination of size and denisity of these biological entities.
Sample: courtesy of Prof. Dr. Torsten Lang, University of Bonn, Germany