Gallery Leica TCS STED

Physiology: muscle research with STED

Rat myofibrils stained with ATTO 647N conjugated antibodies against an N-terminal epitope in the titin molecule (clone T12), which lies at the edges of the z-disc in the sarcomere.

Top: STED. Bottom: Confocal. The scalebar indicates 1 µm.

Courtesy of Dr. Elisabeth Ehler, Kings College, London, UK

Comparison of resolution - STED vs. confocal

Colloidal crystal structure of fluorescent nanospheres ATTO 647N.

Layer of fluorescent nano-beads imaged under optimal confocal conditions (bottom) and using the same settings with the STED-depletion laser turned on (top).

The scalebar indicates 2 µm.

Courtesy of Max Planck Institute for Biophysikal Chemistry, Goettingen, Germany

Membrane domains

Clustering of Syntaxin 1A Nano-domains of the basolateral membrane of PC12 cells. The nanoresolving power of STED micorscopy allows for the determination of size and denisity of these biological entities.

Sample: courtesy of Prof. Dr. Torsten Lang, University of Bonn, Germany