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Objective Classes

Objectives are categorized into performance classes on the basis of their transmission, chromatic correction, planarity, and others.

From the point of view of the International Organization of Standardization (ISO), there are three groups of objective classes differing in quality of chromatic correction.

Objective Class

Field Flatness

Absolute Value(s) of Focus

Application

Achromats

up to 23 mm

between red and blue wavelength (2 colors) is ≤ 2x depth of field

for standard applications in the visual spectral range

Semi-Apochromats

up to 25 mm

for red wavelength and blue to green wavelength (3 colors) are ≤ 2.5x depth of field

for applications in the visual spectral range with higher specifications

Apochromats

up to 25 mm

for red wavelength and blue to green wavelength (3 colors) are ≤ 1x depth of field

for applications with highest specifications in the visual range and beyond

Depth of field: 

Leica Microsystems has aligned all three performance classes of its range of objectives to the needs of its customers and the specific requirements of a wide variety of applications – while offering a convincing price-performance ratio.

Special objectives

For research involving highly specialized techniques or challenging samples, specially optimized objectives are often needed. Leica Microsystems offers a wide choice of special objectives for such applications.

Some examples:

  • TIRF objectives with top numerical apertures
  • Objectives with high transmission around 350 nm for laser microdissection
  • Special objectives for patch-clamp applications on vital brain sections allowing measurements of individual ion channels using an inert dip-in objective
  • Infrared-optimized multiphoton objectives for the visualization of deep-lying neuronal tissue sections
  • Water, glycerol and multi-immersion objectives with the correct refraction index for the sample medium for aberration-free imaging of thicker samples