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Super-Resolution Microscopes and Nanoscopes

Super-resolution microscopes and nanoscopes overcome the diffraction limit of light and allow investigators to study subcellular structures in greater detail than achieved with a standard confocal microscope. With the possibility of resolutions down to 30 nm with STED as well as sub-cellular dynamics can be studied at the nanoscale. Sub-diffraction colocalization analysis reveals interactions unprecedented detail. Enabling novel discoveries to be made in the fields of virology, immunology, neuroscience and cancer, super-resolution is on its way to becoming the new gold standard in light microscopy.

Your entry has never been easier than today with HyVolution 2 and Leica TCS SP8 STED ONE, STED 3X.

Explore life’s true nature in unprecedented detail.

Need Assistance?

Contact a local specialist for advice on the right super-resolution or nanoscopy system for your needs.

You decide which level of detail answers your question

Simulation of red and green entities organized in two rings as seen at different levels of resolution. Data courtesy of Remko Dijkstra, Scientific Volume Imaging Deconvolution powered by Huygens - SVI

HyVolution 2: fast super-resolution for every task

HyVolution 2 is super-resolution for every specimen and every experiment. Study the rapid dynamics of living cells, colocalization, image multiple fluorophores simultaneously, or capture intracellular details with resolution down to 140 Nanometer.

Multiple colors in up to five channels simultaneously

Unlike super-resolution systems that use a single external detection channel HyVolution 2 integrates seamlessly with all confocal imaging modalities. Therefore, you can record all five channels simultaneously – without the need for sequential scanning, which would only slow down the acquisition speed.

Seamless integration with Huygens by SVI: you have the freedom to use fast parallel processing on GPU. HyVolution 2 thus becomes part of the image acquisition process making it the fastest confocal super-resolution system in every respect.

Developed with our partners from SVI.

Pulling the veil from your images: HyVolution 2 reveals minute detail in this 4-channel recording of Cos-7 cells, BodipyFL Phallacidin (Actin), Alexa Fluor 568 F(ab')2 (Tubulin), Alexa Fluor 647 F(ab')2 (Mitochondria), DAPI (nuclei). Sample courtesy of Dr. Jana Döhner, Center for Microscopy and Image Analysis, University of Zürich.



Live cell STED imaging with the new HC PL APO 93X/1.30 GLYC motCORR – STED WHITE. Cytoskeleton in living HeLa cell. Xyz stack with 62 slices confocal/STED planes. SiR tubulin (available from Spirochrome.com). Depth color-coded.

Leica STED: your first choice for nanoscopy

Molecular interaction occurs on the nanoscale, so it is intrinsically difficult to discover with traditional confocal imaging. STED opens the door to the nanoscale, thus providing new insights into molecular interactions. Colocalization, a pre-requisite for the existence of molecular interactions, can be quantified reliably.

The fully integrated STED (STimulated Emission Depletion) systems by Leica Microsystems meet the requirements of daily research and open the door to fast, intuitive, and purely optical access to structural details far beyond the diffraction limit. Resolution becomes tunable in x,y and z with the Leica TCS SP8 STED 3X and TCS SP8 STED ONE.

Nanoscopy in 3D

  • Tunable, direct nanoscopy in x, y and z
  • Multicolor colocalization studies beyond limits
  • Live cell imaging with standard fluorescent proteins like GFP
  • Intuitive software control with Smart STED Wizard
  • Real turnkey due to one-click auto alignment

Driven by your research: twelve years of innovation towards nanoscopy

True to the credo of Ernst Leitz ‘with the user, for the user’, Leica Microsystems has pioneered super-resolution/nanoscopy right form the beginning by collaborating closely with leading scientists such as the Nobel Prize laureate Stefan Hell, as well as partners in industry. This approach continuously improves the performance of our systems and has won multiple awards (e.g. 2014: R&D 100 and The Scientist Top Ten Innovations awards for TCS SP8 STED 3X; the Microscopy TODAY Innovation Award for SR GSD 3D). With well over a decade of providing solutions in this field, Leica Microsystems today offers not only excellent products but also high standards of service and support.

Leica Microsystems super-resolution and nanoscopy products

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Explore life’s true nature with super-resolution and nanoscopy


With HyVolution 2 and the Leica TCS SP8 STED ONE, Leica Microsystems paves the way to access imaging beyond the diffraction limit