Leica Microsystems: Leica Science Lab

Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

How To Create EDOF (Extended Depth of Focus) Images

Watch this video to see how you can rapidly record sharp optical microscope images of samples with a large height variation. This is done with the optional Extended Depth of Focus (EDOF) function of…

Schematic graph of the light path in a Spalt-Ultramikroskop.

Confocal and Light Sheet Imaging with STELLARIS DLS

Optical imaging instrumentation can magnify tiny objects, zoom in on distant stars and reveal details that are invisible to the naked eye. But it notoriously suffers from an annoying problem: the…

FusionOptics in Neurosurgery and Ophthalmology – for a Larger 3D Area in Focus

Neurosurgeons and ophthalmologists deal with delicate structures, deep or narow cavities and tiny structures with vitally important functions. A clear, three-dimensional view on the surgical field is…

Object planes of the Greenough stereomicroscope with depth of field range.

Depth of Field in Microscopy

In microscopy, depth of field is often seen as an empirical parameter. In practice it is determined by the correlation between numerical aperture, resolution and magnification. For the best possible…