STED & GSDIM (dSTORM) Super-Resolution Microscopy
Great insights have been obtained from fluorescence microscopy. The ultimate goal is the study of subcellular architecture and dynamics. However, often the structures of interest are far too small to be resolved using standard fluorescence microscopy. Several approaches to overcome this limitation have been developed over the last decades and super-resolution microscopy has proven an extremely valuable tool not only in life science research.
Leica Microsystems is a pioneer in super-resolution microscopy working closely in collaboration with top scientists in this field. Leica Microsystems is the only supplier of both, widefield super-resolution and confocal super-resolution technology incorporating methods such as STED and GSDIM/dSTORM, respectively.
Widefield Super-Resolution Microscopy / Localization Microscopy
The market launch of the Leica SR GSD widefield super-resolution microscope in 2011 put Leica Microsystems firmly on the road to success in the localization microscopy sector, too. Like the STED system, this was a result of the close cooperation with Professor Stefan Hell of the Max Planck Institute, Göttingen.
The innovative character of the Leica SR GSD - first in 2D – was acknowledged with several awards. The Scientist magazine elected the product as one of the Top 10 Innovations of 2011, R&D magazine gave it a 2012 R&D100 Award as well as only one of three 2012 R&D100 Editor’s Choice Awards and the magazine of the Microscopy Society of America, Microscopy TODAY, honored the Leica SR GSD with the 2012 Innovations Award.
3D Localisation Microscopy
The Leica SR GSD, winner of the R&D100 Award and other prizes, has now been further developed.
The new Leica SR GSD 3D offers not only highly precise and reproducible localization of single molecules in 2D, but also in 3D. The renewed distinction by The Scientist, whose panel of judges put the Leica SR GSD 3D third on their list of Top 10 Innovations in 2013, continues the award-winning series of Leica Microsystems products and underlines the importance of high-resolution microscopy for life sciences.
Far beyond the diffraction limit
Based on the highly regarded Leica AM TIRF MC system, the Leica SR GSD 3D harnesses the technique of Ground State Depletion followed by individual molecule return (GSDIM). This method achieves resolutions of down to 20 nm (lateral) and 50 nm (axial).
- Equipped with a highly color corrected 160x high-performance objective specifically developed for super-resolution microscopy
- Design is optimized for high-power laser emissions
- Extremely low autofluorescence ensures a high signal-to-noise ratio, which is ideal for single molecule detection applications
- GSDIM technology is that it uses standard fluorophores
- Besides the standard calibration with gold beads, color-specific calibrations with fluorescence beads or dye molecules of the specimen are available
- Complete integration of the technology in the LAS AF software platform
- Special suppressed-motion stage (SuMo) for minimum drift and maximum stability
Confocal Super-Resolution Microscopy / STED Microscopy
Leica Microsystems has a long standing experience in super-resolution and introduced the first STED microscope system in 2007. The fully integrated Leica STED solutions are designed to provide fast, intuitive, and purely optical access to structural details – far beyond the diffraction limit. Based on confocal principles, STED microscopy combines intrinsic optical sectioning capability with fast data acquisition and dual color super-resolution. Live cell imaging and colocalization studies below the diffraction limit are easily achieved.
The new Leica TCS SP8 STED offers super-resolution on Leica Microsystems' highly versatile and modular confocal platform Leica TCS SP8. This instrument realizes STED microscopy in the green/yellow range of the spectrum and enables researchers to use conventional dyes such as Alexa 488, FITC and Oregon Green and established fluorescent proteins such as eGFP and eYFP.
Gated STED Option
STED, the Leica HyD™ detectors and the White Light Laser are perfect partners in the innovative gated STED option. Resolution and contrast are increased, whilst laser power is decreased. This enhances photo-stability and means a major step forward in super-resolution for live cell imaging. Sub 50 nm lateral resolution is specified!
Super Resolution Latest News
Leica SR GSD 3D Super-Resolution Microscope Voted Among Top 10 Innovations 2013 for Laboratories and Research
The Scientist Magazine Chooses Super-Resolution Microscope from Leica Microsystems as one of the...
See the full spectrum of life – with the smallest confocal volume ever
Leica Microsystems Releases Leica Application Suite Advanced Fluorescence (LAS AF) 3.2
Leica SR GSD 3D – Highly Precise and Reproducible Localization of Single Molecules and Cellular...
Leica Microsystems Super-Resolution Products
Leica SR GSD 3D
Super-Resolution System for 3D Localization Microscopy
Widefield fluorescence microscope based on GSD (Ground State Depletion) or dSTORM (Direct STochastical Optical Reconstruction Microscopy) technology...
Leica TCS SP8 STED
Opening the Gate to Super-Resolution
Studying subcellular architecture and dynamics with conventional fluorescence microscopy can be challenging as structures smaller than 200 nm are...