The market launch of the Leica SR GSD widefield super-resolution microscope in 2011 put Leica Microsystems firmly on the road to success in the localization microscopy sector, too. Like the STED system, this was a result of the close cooperation with Professor Stefan Hell of the Max Planck Institute, Göttingen.
The innovative character of the Leica SR GSD - first in 2D – was acknowledged with several awards. The Scientist magazine elected the product as one of the Top 10 Innovations of 2011, R&D magazine gave it a 2012 R&D100 Award as well as only one of three 2012 R&D100 Editor’s Choice Awards and the magazine of the Microscopy Society of America, Microscopy TODAY, honored the Leica SR GSD with the 2012 Innovations Award.
The Leica SR GSD, winner of the R&D100 Award and other prizes, has now been further developed. The new Leica SR GSD 3D offers not only highly precise and reproducible localization of single molecules in 2D, but also in 3D. The renewed distinction by The Scientist, whose panel of judges put the Leica SR GSD 3D third on their list of Top 10 Innovations in 2013, continues the award-winning series of Leica Microsystems products and underlines the importance of high-resolution microscopy for life sciences.
Leica Microsystems has a long standing experience in super-resolution and introduced the first STED microscope system in 2007. The fully integrated Leica STED solutions are designed to provide fast, intuitive, and purely optical access to structural details – far beyond the diffraction limit. Based on confocal principles, STED microscopy combines intrinsic optical sectioning capability with fast data acquisition and dual color super-resolution. Live cell imaging and colocalization studies below the diffraction limit are easily achieved.
Leica SR GSD 3D: 3D reconstruction of the synaptonemal complex protein 3 (SYCP3) stained with Alexa 647. Medium: 50 mM MEA in PBS. Five image stacks each 800 nm in thickness were merged. Courtesy of M. Lessard, The Jackson Laboratory, Maine, USA (sample source).
Leica TCS SP8 STED: Histone H3 in HeLa cells visualized by STED and Confocal microscopy.
The Leica SR GSD 3D is equipped with a highly color corrected 160x high-performance objective specifically developed for super-resolution microscopy. The design is optimized for high-power laser emissions. Extremely low autofluorescence ensures a high signal-to-noise ratio, which is ideal for single molecule detection applications. GSDIM technology is that it uses standard fluorophores.
Besides the standard calibration with gold beads, color-specific calibrations with fluorescence beads or dye molecules of the specimen are available. It offers complete integration of the technology in the LAS AF software platform and special suppressed-motion stage (SuMo) for minimum drift and maximum stability.
The new Leica TCS SP8 STED 3X offers super-resolution on Leica Microsystems' highly versatile and modular confocal platform Leica TCS SP8. STED super-resolution meets the requirements of daily research and enables to discover minute details with live cell imaging capabilities. Leica TCS SP8 STED 3X, allows the whole spectrum of visible light and opens the door to super-resolution in all dimensions.
STED, the Leica HyD™ detectors and the White Light Laser are perfect partners in the innovative gated STED option. Resolution and contrast are increased, whilst laser power is decreased. This enhances photo-stability and means a major step forward in super-resolution for live cell imaging. Sub 50 nm lateral resolution is specified!
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