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Color coded three-dimensional image volume of eye specific GFP expression (3P3 promoter) in Drosophila melanogaster. Specimen courtesy of Nadja Dinges, Roignant Lab, IMB Mainz

Principle of Light Sheet Imaging

Light sheet microscopy is a highly suitable way of imaging sensitive samples or fast biological  processes by illuminating the specimen only in a single plane. Since there is no out-of-focus excitation, phototoxic effects can be reduced to the focal plane. It also means that you automatically have optical sectioning and you can image specimens in 3D by moving the sample through the light sheet.

Tracking of Very Fast Periodic Movements

A new xytz scan mode in the LAS X software acquires time series of single planes, which can be fused to a 4D movie afterwards. This allows you to track very fast periodic movements, that are too fast to follow by conventional time-lapse recordings of z stacks – such as a beating zebrafish heart.

Always the Right Laser

All visible lasers of your Leica TCS SP8 confocal are ready to be used for light sheet imaging. Highest flexibility in choosing the right dye for your light sheet experiment offers the white light laser. It even allows you to use spectrally close fluorophores in multicolor experiments.

Superior Optics

Superior objectives for a wide range of applications is one of Leica Microsystems’ hallmarks. The heart of our system turning the light sheet vertically is formed by the objectives and the TwinFlect mirror device. With the choice of two different illumination objectives, the Leica HC PL Fluotar 2.5x/0.07 and the Leica HCX PL FLUOTAR 5x/0.15, you can shape the light sheet depending on your experiments’ requirements. In order to reveal finest details or have a larger field of view, you can pick the optimal detection objective, either the Leica HC FLUOTAR L 25x/0.95 W or the Leica HC APO L 10x/0.3 W water dipping objectives.