Color coded three-dimensional image volume of eye specific GFP expression (3P3 promoter) in Drosophila melanogaster. Specimen courtesy of Nadja Dinges, Roignant Lab, IMB Mainz
Principle of Light Sheet Imaging
Light sheet microscopy is a highly suitable way of imaging sensitive samples or fast biological processes by illuminating the specimen only in a single plane. Since there is no out-of-focus excitation, phototoxic effects can be reduced to the focal plane. It also means that you automatically have optical sectioning and you can image specimens in 3D by moving the sample through the light sheet.