Confocal Microscopes
Modular concept of our confocal microscopes
High-speed imaging supplies the data for a wide range of integrated analytical techniques. Our confocal microscopes are based on a modular concept that enables flexible upgrading and integration of innovative technology all the way to the nano range with STED 3X.
Confocal Microscopes 14
Filter by Area of Application
SP8 LIGHTNING
Super-resolution live cell imaging in multicolor
SP8 FALCON
The contrast is clear. Lifetime imaging in an instant
SP8 DIVE
The first multiphoton microscope with spectrally tunable detection.
LIGHTNING
The maximum of information from each specimen
Leica TCS SP8 CARS
Label-free imaging of native specimens
Leica TCS SP8 STED
STED Nanoscopes
Leica TCS SP8 DLS
Combining Light Sheet and Confocal
Leica TCS SP8 X
Optimal excitation of any dye with white light laser covering the full visible spectrum
Leica TCS SPE
The personal spectral confocal for daily research
Leica HyD
The hybrid detector for super-sensitive imaging and photon counting
Leica DCM8
Digital microscope for 3D surface measurement in production and research
Leica HCS A
High content screening automation
Leica motCORR Objectives
Fast adjustment to varying RI for best optical performance in 3D
RemoteCare
Smart monitoring and service option for your microscope
Confocal Microscopes Latest News
Register to join the world premiere!
GET CLOSER TO THE TRUTH - We have re-imagined confocal imaging. Join the world premiere on the 7th of April at 3 pm Central European Summertime.
End-of-life of Microsoft Windows 7
Upgrade your Confocal Control Workstation. Speak to your Service Engineer or contact our support team to discuss the best option for your confocal...
Leica Microsystems and University Mainz cooperate for a Microscopy Reference Center
New center with focus on innovation for light microscopy is located at University Medical Center in Mainz, Germany
Next generation confocal platform for super-resolution live cell imaging in multicolor
Detailed observations of fast biological processes with the SP8 LIGHTNING
UC San Diego and Leica Microsystems Establish Center of Excellence in Microscopy
The new Center will support a combined mission to educate the next generation of scientists and drive new scientific discoveries
Winners of the Human Protein Atlas Image Classification Announced
Kaggle Competition Prize Money Sponsored by Leica Microsystems
Learn how to Remove Autofluorescence from your Confocal Images
Autofluorescence can significantly reduce what you can see in a confocal experiment. This article explores causes of autofluorescence as well as…
Interview with Magali Mondin on THUNDER Imagers
Magali Mondin, an engineer at the BIC (Bordeaux Imaging Center) in France, describes her experience using a THUNDER Imager in this interview. She had…
Multiphoton Microscopy Publication List
Multiphoton Microscopy is an advanced technique for imaging thick samples. Applications range from the visualization of the complex architecture of…
How to prepare samples for Stimulated Raman Scattering
Stimulated Raman Scattering (SRS) is an emerging microscopy technique that images the vibrations of chemical bonds within a specimen. It allows…
Principles of Multiphoton Microscopy for Deep Tissue Imaging
Basics of multiphoton microscopy. This tutorial explains the principles of multiphoton microscopy for deep tissue imaging. Multiphoton microscopy…
Step by Step Guide to the Molecular Basics of CARS Microscopy
CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic…
New Light Shed on the Nanodomain Organization of the Endoplasmic Reticulum (ER)
The endoplasmic reticulum (ER) is a continuous membrane organelle in charge of protein synthesis, lipid synthesis and detoxification. The ER structure…
Image Gallery: THUNDER Imager
To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using…
Stimulated Raman Scattering Microscopy Probes Neurodegenerative Disease
Despite decades of research, the molecular mechanisms underlying some of the most severe neurodegenerative diseases, such as Alzheimer’s or…
Imaging Intracellular Temperature using Fluorescence Lifetime Imaging Microscopy (FLIM)
This video shows the presentation of Dr. Kohki Okabe given at the 9th Congress of the Federation of Asian and Oceanian Physiological Society (FAOPS)…
About Confocal
Confocal Laser Scanning Microscopy (CLSM) is one of a series of methods to generate slices from microscopic samples by means of optics. The sample stays intact, and the slicing may be repeated many times. True Confocal Scanning (TCS) is a technique, where only a single, diffraction limited spot is illuminated and observed at a time. The benefit of confocal imaging is a dramatically increased contrast by removal of out-of-focus haze. Z-sequences of optical slices (3D image stacks) are sources for subsequent rendering as anaglyphes, depth-coded maps or 3D movies. TCS is also very well compatible with multi-fluorescence imaging, time-lapse imaging, FLIM, FRAP and FCS measurements – plus a whole world of spectral applications.
Learn how to Remove Autofluorescence from your Confocal Images
Autofluorescence can significantly reduce what you can see in a confocal experiment. This article explores causes of autofluorescence as well as different ways to remove it, from simple media fixes to…Read article
Multiphoton Microscopy Publication List
Multiphoton Microscopy is an advanced technique for imaging thick samples. Applications range from the visualization of the complex architecture of the whole brain to the study of tumor development…Read article
Pore Scale Visualization of Drainage in 3D Porous Media by Confocal Microscopy
This work reports new interesting findings concerning porous media infiltration which have direct implications for the fields of energy (oil recovery) and environment (CO2 sequestration and aquifer…Read article
Multiphoton imaging bolsters findings on in vitro differentiation of pluripotent stem cell-derived podocyte
The renal corpuscle of the kidney comprises a glomerular vasculature embraced by podocytes and supported by mesangial myofibroblasts. Their combined actions are essential for the formation of an…Read article
Cellular Motility: Microtubules, Motor Proteins and Tau-Proteins
Cellular motility is based on motor-proteins that can bind to filamentous scaffold proteins and – under consumption of ATP – can “crawl” on these filaments. This note is about proteins connected to…Read article
Visualize developmental patterns in Drosophila
The marvelous complexity of life is perhaps the greatest source of fascination within the biological sciences. Among the most complex subjects within biology is the development of multicellular life.…Read article
Visualize mechanical interactions of Cancer Cells
Cells interact with their environment. Not only on the base of chemical signals, but also by sensing and modifying mechanical properties of the extracellular matrix. The research goal of Dr. Bo Sun’s…Read article
Zebrafish Brain - Whole Organ Imaging at High Resolution
Structural information is key when one seeks to understand complex biological systems, and one of the most complex biological structures is the vertebrate central nervous system. To image a complete…Read article
What is a Resonant Scanner?
A resonant scanner is a type of galvanometric mirror scanner that allows fast image acquisition with single-point scanning microscopes (true confocal and multiphoton laser scanning). High acquisition…Read article
What is a Spectral Detector (SP Detector)?
The SP detector from Leica Microsystems denotes a compound detection unit for point scanning microscopes, in particular confocal microscopes. The SP detector splits light into up to 5 spectral bands.…Read article
Improve 3D Cell Biology Workflow with Digital Light Sheet Microscopy
Understanding the sub-cellular mechanisms in carcinogenesis is of crucial importance for cancer treatment. Popular cellular models comprise cancer cells grown as monolayers. But this approach…Read article
Improve Workflow in Kidney Research with 3D STED Deep Nanoscopy
Visualize the ultrastructure of the glomerular filtration barrier. The combination of optical clearing and nanoscopy allows to study the glomerular filtration barrier at the nanoscale. The Leica TCS…Read article
Resolved Field Number (RFN)
The field number (FN) for optical microscopes indicates the field of view (FOV). It corresponds to the area in the intermediate image that is observable through the eyepieces. Although, we cannot…Read article
What is a Hybrid Detector (HyD)?
A HyD is a sensor for detecting light (photons). It is a compound (hybrid) of two technologies: vacuum tubes, like those used in a photomultiplier tube (PMT), and semiconductor microelectronics, like…Read article
What is a Field-of-View Scanner?
A field-of-view scanner is an assembly of galvanometric scanning mirrors used in single-point confocal microscopes that offer the correct optical recording of large field sizes. The field-of-view…Read article
What is a Tandem Scanner?
A Tandem Scanner is an assembly of two different types of scanning together in one system for true confocal point scanning. The Tandem Scanner consists of a three-mirror scanning base with the…Read article
A Molecular Link between Cell Migration and Vascular Disease
Blood vessels transport vital nutrients and oxygen to all the cells in the body. Guided by a complex signaling network, endothelial cells sprout, proliferate, and migrate to form those vessels. One of…Read article
Kaggle Competition for Multi-label Classification of Cell Organelles in Proteome Scale Human Protein Atlas Data
The Cell Atlas, a part of the Human Protein Atlas, was created by the group of Prof. Emma Lundberg at the SciLifeLab, KTH Royal Institute of Technology, in Stockholm, Sweden. Currently, she is a…Read article
You Shall Not Pass! Time-gated Detection Takes Care of Endogenous Fluorescence in Plant Research.
Yutaka Kodama is Associate Professor at the Biosciences Education Research Center, Utsunomiya University, Japan. He graduated from Saga University, Faculty of Agriculture, in 2004 and received his PhD…Read article
Simultaneously Measuring Image Features and Resolution in Live-Cell STED Images
Reliable interpretation and quantification of cellular features in fluorescence microscopy requires an accurate estimate of microscope resolution. This is typically obtained by measuring the image of…Read article
MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)
Programmed cell death (PCD)-triggered degradation of plant tapetum is essential for microspore development and pollen coat formation; however, little is known about the cellular mechanism regulating…Read article
Which Sensor is the Best for Confocal Imaging?
The Hybrid Photodetectors (HyD) are! Why that is the case is explained in this short Science Lab article.Read article
Image Gallery: FLIM for high-speed investigation of molecular interactions
SP8 FALCON (FAst Lifetime CONtrast) is the fast, and fully integrated platform for fluorescence lifetime imaging (FLIM) and analysis. It enables the observation of fast dynamic cellular events with…Read article
Chemical Basis for Alteration of an Intraocular Lens Using a Femtosecond Laser
The chemical basis for the alteration of the refractive properties of an intraocular lens with a femtosecond laser was investigated. Three different microscope setups have been used for the study:…Read article
mTORC1 Promotes Proliferation of Immature Schwann Cells and Myelin Growth of Differentiated Schwann Cells
The myelination of axons is essential for neuronal wiring and normal nervous system functions. In the peripheral nervous system, Schwann cells (SCs) form myelin sheaths around axons during nerve…Read article
Autocrine Regulation of Stomatal Differentiation Potential by EPF1 and ERECTA-LIKE1 Ligand-receptor Signaling
Development of stomata, valves on the plant epidermis for optimal gas exchange and water control, is fine-tuned by multiple signaling peptides with unique, overlapping, or antagonistic activities.…Read article
High-Resolution 3D Imaging of Whole Organ after Clearing
Zebrafish testis has become a powerful model for reproductive biology of teleostean fishes and other vertebrates and encompasses multiple applications in applied and basic research. Many studies have…Read article
KChIP2 regulates the cardiac Ca²⁺ transient and myocyte contractility by targeting ryanodine receptor activity
Pathologic electrical remodeling and attenuated cardiac contractility are featured characteristics of heart failure. Coinciding with these remodeling events is a loss of the K+ channel interacting…Read article
IT Security Whitepaper
Security is a primary concern of Leica Microsystems and Leica Microsystems’ customers that employ remote services. Leica Microsystems requires a proven remote service solution that protects against…Read article
Expression Analysis of Platelet‐derived Growth Factor Receptor Alpha and its Ligands in the Developing Mouse Lung
Activation of the platelet‐derived growth factor receptor‐α (PDGFR α) signaling pathway is critically important during lung alveogenesis, the process in lung development during which alveoli are…Read article
Useful Links
Communities and Web Sources
www.researchgate.net/Social network for scientists
www.lsoft.com/scripts/wl.exe?SL1=CONFOCALMICROSCOPY&H=LISTS.UMN.EDUConfocal Microscopy Mailing List, University of Minnesota
www.ibiology.org/Teaching tools, video lectures on biology and microscopy
www.ibiology.org/Teaching tools, video lectures on biology and microscopy
bitesizebio.comOnline magazine and community for molecular and cell biology researchers
www.somersault1824.comResource for high-end scientific illustrations, images and animations
Search Engines and Data Bases
www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells
harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins
www.gopubmed.comSearch interface for pubmed
en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines
scholar.google.comBeta of Google's search engine for scientific article abstracts
Journals
www.doaj.org/Directory of open access journals
emboj.embopress.org/The EMBO Journal
www.lifescied.orgCBE-Life Sciences Education – an ASCB online journal
www.sciencemag.org/Science
www.nature.com/Nature
www.cell.com/Biweekly publication of exceptional research articles
jcs.biologists.org/Journal of Cell Science
dev.biologists.org/Development
jeb.biologists.org/The Journal of Experimental Biology
dmm.biologists.org/DMM Disease Models & Mechanisms
www.biotechniques.com/International Journal of Life Science Methods
www.opticsinfobase.org/Collection of Journals and Proceedings in Optics and Photonics
spie.org/x576.xmlSPIE - peer-reviewed journals on applied research in optics and photonics
onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648Journal of Biophotonics
www.plosone.org/home.actionInternational, peer-reviewed, open-access, online publication
rspb.royalsocietypublishing.org/Proceedings B - the Royal Society's biological research journal
www.microscopy-analysis.com/International Journal for microscopists
Organizations
www.microscopy.org/Microscopy Society of America
www.eurmicsoc.org/European Microscopy Society
www.rms.org.uk/Royal Microscopical Society
www.ascb.org/ASCB American Society of Cell Biology
www.biologists.com/cob_activities.htmlthe company of biologists
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