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Enjoy spectral freedom with 4Tune

The number of transgenic and synthetic markers evolves constantly and rapidly. With the STELLARIS 8 DIVE you can keep up with these developments and adapt to existing and new fluorescent markers with just a few mouse clicks!

Spectral freedom results from the patent-pending, groundbreaking 4Tune technology for non-descanned detection. 4Tune detection allows you to adapt to the full emission and to separate strongly overlapping spectra.

The STELLARIS 8 DIVE enables you to optimally adapt to the emission of your markers – you can capture twice as much of the fluorescence signal, improve penetration depth, imaging speed and reduce phototoxicity for in vivo imaging.

The conventional dichroics are never optimal to distinguish all fluorophores, but with the spectral detectors, this is now possible and much easier, since we can really optimize for each fluorophore the wavelengths you want to detect.

Prof. Dr. Jacco van Rheenen. Netherlands Cancer Institute, Amsterdam (the Netherlands).

Small intestines of a confetti mouse, fluorescently labeled and lineage traced from a multi-color tracer. The gray color codes for the SHG signal from Collagen. Lineage traced stem cells are shown in cyan (CFP), green (GFP), yellow (YFP) and red (RFP). Image size is ca. 700x700x150 μm³. Recorded with two-photon excitation, using the STELLARIS 8 DIVE. Sample courtesy of J. van Rheenen, Netherlands Cancer Institute, Amsterdam (the Netherlands).

DIVE with ease – the 4Tune detector

The 4Tune non-descanned detection system can be equipped with 2 to 4 detectors and is freely configurable with hybrid detectors (Power HyD NDD), photomultipliers (PMTs), or a mix of both. The emission light is separated by a combination of variable dichroics and bandpass filters. Freely tune your detection over the whole visible spectrum (380 – 800 nm)!

The 4Tune user interface allows you to optimize the emission setting for multiple transgenic markers by simple drag and drop. Due to its clear and intuitive design, operating it is easy and requires minimal training.

With the STELLARIS 8 DIVE, you are equipped for every existing and newly developed transgenic marker and future proved for new developments!

Top: 4Tune non-descanned detection system: 1) Variable Dichroic (VD). 2) Variable Bandpass (VB). 3) Power HyD NDD or PMT. Bottom: The intuitive 4Tune user interface allows easy setting of the detection windows for all colors from 380 up to 800 nm.
Mouse brain cortex, Thy1-eYFP. 20% improved penetration depth using Best Depth setting. IRAPO 25x1.0 W motCorr. Sample courtesy of Kevin Keppler, Light Microscope Facility, DZNE Bonn (Germany).

Explore new dimensions in depth

With the STELLARIS 8 DIVE, you can tune for the deepest insight and finest detail. All excitation beams can be optimally adjusted independently for any objective using the new Vario Beam Expander (VBE).

The VBE allows for optimized colocalization and the right balance of resolution and depth in line with your research question.

Optimize for depth and resolution with the Vario Beam Expander

The Leica Vario Beam Expander VBE combines tunable beam diameter and tunable divergence. This offers you maximum depth, best resolution and full color correction.

Tunable beam diameter for best balance of resolution and depth

The STELLARIS 8 DIVE allows you to adapt to your sample requirements. Using the Vario Beam Expander, you can choose: Maximum resolution – which results from a fully illuminated back aperture of your objective lens – and maximized penetration depth – resulting from slightly underfilling the back aperture. Underfilling the back-aperture results in a larger focal volume and a reduced pathlength which leads to more efficient excitation.

Tunable beam divergence for full color correction

Our IR APO objectives do not suffer from chromatic aberations over the IR range. However, with STELLARIS 8 DIVE you are ready to use objective lens suitable for IR along with multiple IR laser lines: The Vario Beam Expander can be used to correct for chromatic shifts and enable meaningful multicolor experiments.

Tunable Vario Beam Expander (VBE)

Reproducible results for multicolor deep in vivo imaging

Excite multiple transgenic markers in a single experiment with color separation. Or even do localized high precision photomanipulation and simultaneous imaging in a diffraction limited volume. The STELLARIS 8 DIVE can be equipped with up to three excitation lines simultaneously. With lasers tunable up to 1300 nm, you can even use red and far red dyes for multiphoton experiments. With higher wavelengths, less scattering enables deeper penetration and results in bright images full of detail from deep layers.

Meaningful multicolor experiments require stable conditions. Great mechanical stability combined with a beamcatcher ensure reliable performance. The beamcatcher easily accounts for drifts that are not in your hands – laser tuning, temperature fluctuations or construction work next door – the beamcatcher restores the overlay of IR and visible laser lines by easy software operation. For the most precise alignment expert users can even fine tune more – deep in vivo co-localization becomes feasible!

Confetti mouse small intestines, fluorescently labeled and lineage traced from a multi-color tracer. The gray color codes for the SHG signal from Collagen. Lineage traced stem cells are shown in cyan (CFP), green (GFP), yellow (YFP) and red (RFP). Image size is ca. 700x700x150 µm³. Recorded with two-photon excitation, using the SP8 DIVE. Sample courtesy of J. van Rheenen, Netherlands Cancer Institute, Amsterdam (the Netherlands).

Get more information from your sample with fluorescence lifetime

4Tune can completely take advantage of fluorescence lifetime imaging (FLIM) using STELLARIS FALCON (FAstLifetime CONtrast), and TauSense.

This additional flexibility adds a new dimension to multiphoton imaging, enabling signal multiplexing and metabolic imaging.

Download TauSense Application Note

Fluorescence intensity image
FLIM image

Autofluorescence multiphoton imaging of wt zebrafish embryo. Lifetime contrast is given by different cofactors and vitamins. In this case NADH (free and protein bound), retinoids and FADH (free and protein bound). Excitation: 740 nm. Emission: 501 - 580 nm. Sample courtesy: Francesco Cutrale, University of Southern California, Los Angeles.

Spatial flexibility for behavioral research

For behavioral experiments, equip your STELLARIS 8 DIVE with the DM8 CS microscope stand with extended workspace. The DM8 CS is mounted on a cascaded table which can be customized to your experimental requirements. The microscope stand provides you with the spatial flexibility for large and complex setups.

The STELLARIS 8 DIVE with the DM8 CS microscope stand enables you to perform experiments such as the monitoring of brain activities on awake animals.

The STELLARIS 8 DIVE with the DM8 CS microscope stand, which is mounted on a cascaded table, offers extended workspace for behavioral research.

The SP8 DIVE with the DM8 CS microscope stand, which is mounted on a cascaded table, offers extended workspace for behavioral research.

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  • Leica Microsystems Inc.
    1700 Leider Lane
    Buffalo Grove, IL 60089 United States
    Office Phone : +1 800 248 0123
    Fax : +1 847-236-3009

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