Fluorescence microscopy is essential technique for studying biological processes. Fluorescence can be used to visualize specific subcellular structures in fixed cells, and reveal connections between dynamic processes in live cells and tissues. Without fluorescence labeling, few cellular process can be visualized since most biological samples are almost transparent. A broad range of advanced Live-cell Imaging Techniques can be employed by researchers to probe the complexities of cellular processes. Fluorescence imaging is central to many of these imaging techniques.
To obtain excellent fluorescence images you need a highly sensitive camera delivering a high signal-to-noise ratio and a large dynamic range resulting in a crisp fluorescence signal. Live-cell imaging often requires a high acquisition speed to capture fast dynamic processes.
We base our range of fluorescence cameras on highly sensitive CCD or sCMOS sensors that are ideally suited for low light applications that will capture even weak signals. The majority of our cameras are actively cooled to help reduce noise. We also use sensors with high quantum efficiency so that you can reduce exposure times, protecting your samples from photo-damage.
Our monochrome and color cameras are designed for a broad range of applications, from basic tasks such as documentation of immuno-stained samples to advanced demanding live cell imaging applications such as fast time-lapse and FRAP. Our Leica Application Suite (LAS) X software seamlessly integrates our cameras into the microscope system, supporting high-speed triggering.
The discovery of fluorescent proteins revolutionized the world of life sciences in the last century and was the key breakthrough technology that permitted the discovery and study of numerous cellular processes. The article traces the history of the fluorescent proteins.
In the beginning, there was GFP, today there is a whole pallet of fluorescent proteins to work with. Get an introduction to fluorescent proteins and their spectral characteristics in this Science Lab article.
|Leica DFC9000 4.2 MP cooled monochrome fluorescence scmos camera for advanced applications like high-speed live cell imaging, FRAP, and ratio measurement||Leica DFC7000 T 2.8 MP cooled color camera for brightfield and fluorescence documentation of fixed cells and tissues||Leica DFC3000 G 1.3 MP monochrome camera for basic fluorescence applications such as documentation of fixed, immunostained samples||Leica DFC365 FX 1.4 MP cooled monochrome camera for standard live cell imaging of GFP-expressing cells and tissues|