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Leica Application Suite

Microscope software platform Leica Application Suite

Free Webinar On-Demand: Basics in Component Cleanliness Analysis

For automotive manufacturers and auto parts suppliers, obtaining component cleanliness results rapidly, accurately, and reproducibly over the entire workflow is a significant advantage. Making it easier to build up facilities, knowledge and expertise in component cleanliness Pall and Leica can offer unique workflow solutions fitting to individual needs and compliant with common standards.

Free Webinar On-Demand: Rate the quality of your steel

The quality of steel is an essential topic in automotive, metalworking and building industries. To ensure the highest standards, an accurate and reliable Quality Assurance workflow for the inspection of non-metallic inclusions is crucial.

Free Webinar On-Demand: Analyze grain size on microstructures the way you need

Grain size plays an essential role in the mechanical properties of materials. Leica Microsystems offers state-of-the-art grain detection technology which automatically enhances and accurately detects grain boundaries, with high flexibility to adapt to specific requirements in any laboratory.

Chronic inflammation under the microscope

This article explains how widefield light microscopy helps to examine the underlying processes of chronic inflammation from a cellular level to whole organisms.

Pharma software - free download of LAS X

Simplify complex analysis tasks and save time by utilizing Leica Application Suite X software integrated with Leica microscopes. LAS X guides you step-by-step through the entire analysis workflow with an intuitive interface for acquisition of multidimensional experiments and powerful analysis wizards.

Leica Microsystems’ modular, easy-to-use microscopes, cameras, and software for drug discovery and research accelerate your workflow by increasing reproducibility and minimizing the need for repeating assays. This allows you to focus on moving your drug to market.

Download free pharma software LAS X

Contact us for more information on LAS X microscope software

Microscopy imaging solutions for pharmaceutical applications

LAS X Core Offline software offers the following functionalities

  • Full image viewer including annotations, image overlay, image gallery, and image comparison
  • Review capability for all acquisition parameters
  • Project tree for data management: save, rename, copy, delete, export as tiff, jpeg, QuickTime, avi, mpeg4, wmv, ASCII
  • Access to project conditions for exporting as xml or applying to another experiment

Image Processing & Measurement

  • Adjust contrast, brightness, and gamma on every image
  • Merge, crop, and image arithmetic
  • Intensity, length and area measurements
  • Measurement of area intensities through image stacks
  • Parallax correction

Modular Structure 

  • Possibility to add additional application modules, fully integrated into core application
  • Additional modules will be protected by a hardware license key

Request a demo, quote or ask a question on LAS X microscope software

LAS X Core Offline software operates without the need for a dongle.

Microsoft Windows 10

Recommendation not to upgrade to Windows 10

If you use Leica Application Suite (LAS), LAS X, LAS AF, or LMD software and wish to upgrade to Windows 10, please contact our service team.

 

Leica Laser Microdissection Software V7.6 release

Leica Laser Microdissection Software V7.6 release

Leica Laser Microdissection Software V7.6 release

We are happy to release our new Leica LMD software V7.6 introducing a new definition of rapid dissection: the Shadow Cut mode (patent pending).

LMD Software Highlights

  • Shadow Cuts: define shapes while the laser is dissecting à this is the fastest way to apply laser microdissection ever. Leica LMD Software Video
  • Support of Cameras Leica DFC7000 T, Leica DFC3000 G and Leica DMC 2900: select your camera of choice out of our portfolio of fast and high-sensitive cameras to enjoy the full power of the system as an upright research microscope or laser microdissection system.
  • Improved AVC Professional: time saving by applying the famous LMD pattern recognition software where needed only
  • … many more features are available, get in touch with your local Leica support to learn more!

Leica LMD Software Product Page

The Leica WF FRAP Photobleaching Module for the Inverted Research Microscope Leica DMi8 is made for the investigation of cellular dynamics and enables users to flexibly define their bleaching area using masks of various sizes and shapes.

The Leica WF FRAP Photobleaching Module for the Inverted Research Microscope Leica DMi8 is made for the investigation of cellular dynamics and enables users to flexibly define their bleaching area using masks of various sizes and shapes.

FRAP Device for Widefield Microscopy

Leica WF FRAP Photobleaching Module for the Inverted Research Microscope Leica DMi8

Wetzlar, Germany. Leica Microsystems launches the Fluorescence Recovery After Photobleaching (FRAP) device for widefield microscopy, Leica WF FRAP. The device is made for the investigation of cellular dynamics and provides users the flexibility to define their bleaching area using masks of various sizes and shapes. An addition to the inverted research microscope Leica DMi8, the Leica WF FRAP module includes a built-in 1.4 Watt laser (450 nm) and is controlled via the imaging and analysis software Leica Application Suite X (LAS X). A dedicated application wizard that enables control of all bleaching parameters guides users through their experiments.

Fluorescence Recovery After Photobleaching experiments investigate the characteristics of molecular interactions by selectively bleaching a defined area of a cell and monitoring the recovery kinetics of fluorescent molecules. The Leica WF FRAP module utilizes the Infinity Port, an additional fluorescence port of the inverted research microscope Leica DMi8, so that the laser has direct access to the infinite light path for performing FRAP experiments.

The complete package includes a dedicated WF FRAP wizard in the imaging software LAS X which facilitates experimental set-up with controls for laser power, pulse duration and time-course parameters. Users can choose from 12 different bleaching masks and therefore fit the bleaching area to their region of interest which may take the form of a single point as well as a larger area of a cell. After every experiment, automated fluorescence recovery curve generation enables direct analysis of the results.

Product Manager Dr. Jennifer Horner comments: “The Leica WF FRAP module brings powerful laser capabilities for photobleaching to live cell imaging systems without added complications. Researchers can delve into the molecular interactions within their cells with ease. Especially when fast kinetics need to be tracked, the Leica WF FRAP is the perfect device to capture fluorescence recovery instantaneously.”

Compared to scanning-based FRAP approaches, the Leica WF FRAP minimizes photostress and offers higher imaging speeds using advanced state-of-the-art cameras. In one all-inclusive module, the Leica WF FRAP gives researchers a tool to study the dynamics of living cells along with the Leica DMi8.

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Leica Microsystems launches the new, user-friendly imaging and analysis software platform for widefield, confocal and super-resolution microscopy

Leica Microsystems launches the new, user-friendly imaging and analysis software platform for widefield, confocal and super-resolution microscopy

New User-Friendly Imaging and Analysis Software Platform for Life Science Research

September 19, 2014

LAS X for Widefield, Confocal and Super-Resolution Microscopy

Wetzlar, Germany. Leica Microsystems launches the easy-to-use Leica Application Suite X (LAS X) imaging software for life sciences spanning all widefield, confocal and super-resolution platforms. LAS X introduces new features for microscopic image acquisition, processing and analysis while maintaining established principles of its predecessor software LAS AF, such as the workflow-based approach. LAS X guides researchers intuitively through image acquisition, data recording and evaluation in live cell imaging, providing for a maximum of reproducibility as customized system settings can be saved. Its basic version is called LAS X Core and provides numerous functions.

Further modules for specific applications such as high content screening, FRET, FRAP, multi-dimensional analysis, colocalization and measurements can be added. To further simplify widefield system usage an Easy Operation Mode is available where users can design their own user interface layout. The Dye Assistant for confocal microscopes helps select the right acquisition settings by just entering the used fluorescent markers.

Product Manager Markus Schechter comments “In developing the new imaging software platform LAS X, we have focused on the user-friendliness of the software. We have taken common workflows most users know and follow into account and translated them into software and this way continued the workflow-based approach of the predecessor LAS AF.” Product Manager Petra Haas adds: “Our software is designed to facilitate the users’ research – experiments are broken down step by step so that researchers can set up experiments more easily. That is the philosophy behind LAS X.”

The 3D and the 2D image analysis wizards generate reproducible analysis results from multi-dimensional data sets. Users are guided through the workflow step by step, from applying filters, thresholding, binary image processing through measurements and classification. Analyses between separate channels can be combined to obtain object-specific data. For example, a binary reference mask can be applied to count the number of spots in each nucleus. Within the 2D Analysis wizard, tracking experiments can be performed, and image analysis protocols can be combined with ImageJ macros. To save time, repetitive analysis protocols can be stored and applied to several experiments. Analysis results are saved with the experiment or can be exported to Excel for further investigation. The LAS X measurement function measures as well as documents distances, areas, volumes, angles and many other parameters.

Dedicated to live cell applications, the hardware-based Adaptive Focus Control (AFC) and the software-based autofocus are integrated in LAS X. Experimental conditions such as temperature, CO2 and O2 are documented and controlled with the Environmental Control module. For long-term time-lapse and screening experiments, the software-controlled micro dispenser compensates evaporation of water immersion. The MotCorr device can be used for software-controlled correction of image aberration. With the Mobile Connection module users can connect to the acquisition station via web client or mobile device and see the course of their whole experiment, review it, or open any image series available in the experiment tree. Experiments can also be started, paused or stopped remotely.

The basic version LAS X Core comes with features such as microscope control, full image viewer capability, diverse processing and quantification tools, movie export, the ability to recall imaging parameters and many more.

To experience the full potential, all optional modules are available as a 30-day-free trial version. For review purposes, there will also be a free-of-charge offline version of LAS X Core to download, providing image processing opportunities independent from the microscope workstation. 

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Leica Microsystems launches the inverted research microscope platform Leica DMi8

Leica Microsystems launches the inverted research microscope platform Leica DMi8

Leica Microsystems Introduces the Inverted Microscope Leica DMi8

September 08, 2014

One Universal Platform for Life Science Applications

Wetzlar, Germany. Leica Microsystems is launching the Leica DMi8 inverted microscope with built-in modularity that can grow and adapt to ever-changing research needs. With this new concept, life scientists can custom-configure an inverted microscope now and upgrade it in the future for applications ranging from basic imaging to advanced fluorescence microscopy. The Leica DMi8 is equipped with an additional incident illumination port, the Infinity Port, which facilitates the integration of additional light sources and laser systems for advanced applications. The closed-loop focus drive with an accuracy of 20 nm for the 12 mm travel range enables researchers to investigate large specimens with high precision. In combination with Leica Microsystems’ imaging software the Leica DMi8 is an optimal solution for live-cell microscopy.

“The Leica DMi8 is an open and freely configurable inverted research microscope which meets the current and future needs of life scientists in a single platform,” explains Leica Microsystems Product Manager Bernard Kleine. “Universality and individuality have been the heart of our development work. With the Leica DMi8 we serve customer needs ranging from plain microscopy to advanced research applications, and pay heed to the fact that tasks and applications in research change. The Leica DMi8 combines the versatility of an open platform with the user-friendliness of a system solution.”

The Leica DMi8’s Infinity Port is the core of the system and enables access to the infinite light path. It integrates light sources and lasers that are necessary for FRAP, photo-switching, ablation, and many state-of-the-art microscopy techniques. Combined with Leica Microsystems’ imaging software, the Leica DMi8 inverted microscope platform enables researchers to document fixed specimens or watch and manipulate living cells quickly and reliably, with outstanding flexibility.

Additionally to the Infinity Port, the Leica DMi8’s closed-loop focus is another innovation. With an accuracy of 20 nm over a 12 mm travel range, researchers can investigate large specimens with high precision. Equipped with 19 mm field of view for sCMOS cameras and 25 mm field of view for optical observation, the Leica DMi8 is designed to collect a maximum of information.

Offering the full range of contrast methods from Brightfield, Integrated Modulation Contrast, Phase Contrast to Darkfield, Differential Interference Contrast, and Fluorescence, the Leica DMi8 is prepared for any microscopy application in life science research. Users can build their individual system by connecting dedicated accessories from Leica Microsystems or third party companies such as light sources, lasers or cameras to meet the changing needs of science. The Leica DMi8’s unique, user-friendly microscope design offers seamless connectivity so researchers can tailor the microscope to their individual requirements. 

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