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Leica Application Suite Advanced Fluorescence LAS AF

Leica Application Suite Advanced Fluorescence (LAS AF) is the easy to use software platform for advanced life science research with Leica Microsystems confocal and widefield systems. LAS AF can be extended by High Content Screening Automation Packages, Leica HCS A, and by additional software packages to exactly match the user’s needs.

Integrated Platform for Advanced Life Science Research

LAS AF offers full control over the microscope hardware and at the same time minimizes the need for user interaction through intelligent automation, e.g. by opening and closing shutters according to the selected contrasting method or by moving matching DIC prisms into the light path when changing objectives. All system parameters can be applied to further experiments for maximum reproducibility.

A wide choice of hardware components e.g. the unique water immersion micro dispenser, high precision stages and live cell accessories can be added to obtain integrated and reliable system solutions dedicated to specific applications like screening, ratio imaging, FRET, colocalization studies, super-resolution, TIRF or electrophysiology.

The experiment data can be reviewed directly on the system or just on a separate PC with one of the offline packages.

Leica Application Suite Advanced Fluorescence Products

Leica LAS AF 3D Analysis Leica LAS AF 3D Visualization
Leica LAS AF 3D Analysis 3D Interactive Measurements and Wizard for Automatic 3D Analysis

With the software module Leica Application Suite Advanced Fluorescence (LAS AF) 3D Analysis you can measure various aspects of intracellular...

Leica LAS AF 3D Visualization True 3D Real-time Rendering with GPU-based Processing

The Leica Application Suite Advanced Fluorescence (LAS AF) software module visualizes samples in three dimensions. It offers a wide variety of...


LAS AF on Widefield Systems

LAS AF can be combined with inverted and upright research microscopes, stereo and fixed stage microscopes, macroscopes and clinical routine microscopes to a fully integrated AF6000 widefield imaging system.

The modular structure of LAS AF for widefield systems allows to combine the core software with exactly what is needed for your experiments: multi channel acquisition, timelapse, z-control and autofocus, mark and find, stitching or additional camera drivers. 

Zebrafish embryo

Courtesy Darren Gilmour, EMBL Heidelberg

"Co-migration of the zebrafish mechanosensory lateral line placode (green) and innervating axons (red) at 36 hours post fertilisation.  Living embryo is a double transgenic carrying neuronal-specific and  placode-specific constructs (NBT:dsRedExpress and claudinB:mGFP,  respectively)". 

z-stack 200 slices/80µm. Processing steps: dye separation and deconvolution. 

Drosophila adult

Courtesy of Yuya Ohhara , Prof. Satoru Kobayashi, National Institute for Basic Biology, Division of Developmental Genetics, Japan

3D reconstruction of the Drosophila central nervous system and intestine. Neuroendocrine cells (green) in the pars intercerebralis  projecting to the intestine. Actin filaments were stained with Phalloidin (violet). 

z-stack 240 slices/83 µm. Processing steps: Blind deconvolution 

LAS AF on Confocal Systems

The Control Software for Leica TCS SP8 already contains all basic tools necessary for advanced imaging application with the confocal system.

To take full advantage of the spectral imaging possibilities of the Leica TCS SP8, it can be extended by the Dye Finder package. 

Advanced Software Packages

To extend the range of applications for both widefield and confocal systems, optional packages are offered for Colocalization Analysis, Calcium Imaging, Electrophysiology, Triggering and complex timelapse experiments, FRET, photobleaching, 3D Visualization with real time rendering, Deconvolution.

All application, processing and quantification modules are identical for all platforms and are fully integrated into LAS AF for fast handling and visualization of large data sets. 

3D reconstruction of a mouse kidney glomerulus using the LAS AF 3D Visualization module. Red, Alexa Fluor© 568 phalloidin, stains filamentous actin in glomeruli and the brush border; green, Alexa Fluor© 488 wheat germ agglutinin, a lectin to label membranous structures of the glomeruli and convoluted tubes; blue, DAPI, stains the nuclei of the cells. 

High-Content Screening with Leica HCS A

Amplify the Power of Imaging

The combination of high-resolution true confocal point scanning technology or integrated AF6000 widefield systems with the ingenious Leica HSC A automation software for the first time offers high content screening on research microscopes.

Run more experiments in less time and save costs. Obtain more research information and statistically relevant data.

Leica HCS A provides great flexibility for individual experiments and unbiased image acquisition. It ensures the necessary freedom demanded by high throughput research.

Review Software

Review and analyse your experiments offline

Take full advantage of your acquisition system by reviewing the experiment in your office. This will free the imaging system for further experiments. 

Cultured hippocampal astrocytes from 18-day-old embryos of Sprague-Dawley rats (Objective lens: Plan APO 20x0.7, 4 Ratio/sec) Courtesy of Drs. Kazunori Kanemaru and Masamitsu Iino, Department of Pharmacology, Graduate School of Medicine, The University of Tokyo, Japan