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Microscope Software Platform LAS X Life Science

Whole slide acquisition in high speed using the Quantum Stage

A quantum leap in high speed acquisition.

High speed acquisition with 5x objective and DFC7000 T, full frame. Slide courtesy of IGBMC, France.

Reconstruction of a zebrafish embryo in 3D

3D reconstruction of a zebrafish lateral line primordium (green) with adjacent neurons (red), muscles (gray) and nuclei (blue) gives insight into spatial organization during embryogenesis. Courtesy of Lionel Newton, EMBL Heidelberg, Germany.

Innervation of a zebrafish embryo

The lateral line (green) of a zebrafish gets innervated (red) during embryo development visualized by time-lapse fluorescence microscopy. Courtesy of Darren Gilmour, EMBL Heidelberg, Germany.

Activity of neuroendocrine cells in drosophila

Neuroendocrine cells (green) of an adult fruit fly are organized in the pars intercerebralis and reach out to the intestine. Actin filaments are depicted in purple. Courtesy of Dr. Satoru Kobayashi, University of Tsukuba, Japan, Dr. Yuya Ohhara, Dr. Kimiko Kobayashi, University of Shizuoka, Japan

The LAS X Image Analysis - An introduction to the 3D Image Analysis Workflow

This Leica LAS X image analysis video tutorial provides users with an introduction on how to perform threshold-based image analysis using the LAS X 3D image analysis module. It provides an orientation on how to open the 2D and 3D analysis modules, the software/user interface layout, and the 10-step workflow for analyzing images.  The main steps in this workflow include image selection, pre-threshold filtering, adjusting thresholds, performing binary image filtering, binary editing, filtering object size, and exporting your data as a spreadsheet.  To demonstrate this functionality, we use an application for counting and measuring fluorescently-labeled nuclei in an image field. This software module can also be used to analyze color images as well as fluorescent (monochrome) images. In addition to counting, other common applications are quantifiying intensity and size/area. 

LAS X File Management - Using the Open Projects tab to better manage .lif files

Basic overview of the Open Projects tab, .lif files, renaming and deleting files, and how to autosave.