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Product Logo LAS X

Leica Application Suite X

Leica Application Suite X (LAS X) is the easy to use software platform for advanced life science research on Leica Microsystems confocal, widefield and super-resolution systems.

Leica LAS AF Sreenshot
Leica LAS AF Sreenshot
Leica LAS AF Sreenshot
Leica LAS AF Sreenshot

The Power of LAS X

Intuitive, Innovative, Indispensable.

The Leica Application Suite X (LAS X) combines the most powerful features available today in microscope software into one package, focusing on usability in every aspect of the interface, functionality and workflow.

The workflow oriented design and the possibility to recall user defined system settings makes LAS X a very intuitive imaging platform customized to your needs. Combining innovative software and hardware options like the Mobile Connection module, the LightGate and Lambda Square Mapping on White Light Laser Systems or the water immersion micro dispenser makes LAS X an indispensable solution for confocal, widefield and super-resolution microscope systems.

  • Analysis of Multi-Dimensional Data Sets
  • Advanced Environmental Control
  • Monitor your experiment anywhere at any time

The Power of LAS X

Guided Image Analysis at its Best

Easy and Versatile Wizards for 3D and 2D Analysis of Multi-Dimensional Data Sets

Obtain reproducible analysis results rapidly and easily with the 3D and 2D image analysis wizards. Go step by step through the guided workflow: from applying filters, thresholding, and binary image processing to measurements and classification. Use a binary reference mask in multi-channel image analysis, for example, to count the number of spots in each nucleus. In addition, you can perform 2D tracking experiments or call ImageJ macros from within the 2D analysis wizard.

Save the analysis results with the experiment or create a report including histogram and binary masks and export it to Excel for further analysis.

For repetitive tasks, user-defined protocols can be saved and applied to new data sets. To manage large amounts of data, several data sets can be added to a batch list and processed at once. Results from different images can also be accumulated, providing statistical information.

Your Advantages

guided workflow screenshot

Guided Workflow

Create reproducible results step-by-step using the Analysis workflow. At each step a set of analysis tools are available. The viewer provides immediate feedback on the applied settings. Additionally, tools such as auto threshold, split objects, or edge removal can be used for automated analysis of multiple samples. When needed, you can set pauses during an automated analysis to allow you to make fine adjustments, for example for binary image editing.

Interactive Measurement Table

Get to the data you need with the flexible and interactive measurement table. Select objects in the image viewer and the corresponding measurement is highlighted in the results table, or vice versa. Choose from a large number of measurement parameters and rearrange or sort the columns as you like. Export measurements and statistics to Excel or save them with the experiment. Reproduce measurements easily with user defined protocols.

interactive measurement screenshot
multichannel analysis examples

Multi Channel Analysis made easy

With multi-channel analysis, even complicated fluorescent experiments can be evaluated with ease. Create separate analysis channels for each fluorescent marker and assign an individual workflow to each. Then simply run an analysis of all aspects of your sample at the same time.

Combine analyses between separate channels to get object specific data. For example, apply a binary reference mask to count the number of spots in each nucleus.

Alternatively, apply multiple analysis channels to the same image. On a stained color image you can count the healthy cells in one analysis channel and create a second analysis channel to count the abnormal cells, for example.

automatic classifier screenshot

Automatic Classifier

Get unbiased and reproducible classification results even in situations where thresholding or filtering is not suitable. Use the intelligent classifier to group your results and display them in a histogram. The classifier can be trained with a training data set, then saved and applied to new data sets. The new objects will be divided into your predefined classes with a support vector machine.

Follow your particles

Perform 2D tracking within the 2D Analysis wizard. Display particle trajectories, follow the objects along their trajectories over time and export the tracking results to excel.

2D tracking

Environmental Control

Make Temperature, CO2 and O2 Conditions Part of your Experiment

Have full control of your experimental conditions with the LAS X Environmental Control module. Monitor and log the environmental data during an experiment. Control all environmental conditions within one interface and run temperature profiles, for example for heat shock experiments.

Your Advantages

climate control screenshot

Full climate control of your experiment

All environmental data is saved with the experiment and can be reviewed subsequently. Know when changes in temperature, CO2 or O2 conditions occur that might affect your experimental results.

Run temperature profiles

In combination with LAS X Live Data Mode, macros can be generated to run temperature profiles. Acquisition can be set to start once a predefined temperature is reached. During the waiting time, a message displays the difference between nominal and actual environmental parameters. Alternatively, you can define that acquisition commences during ramping up of the temperature.

temperature profiles screenshot
temperature warning settings screenshot

Get a warning when parameters are out of range

All environmental parameters are set within one interface. Activate range control to receive a warning when the upper or lower limits are exceeded. If needed, the experiment can be programmed to stop automatically once a limit is passed.

Monitor your experiment anywhere at any time

Don´t miss anything! With the LAS X Mobile Connection module you can stay connected to your experiment anywhere at any time. Display the imaging conditions on your remote device and review your experiment or download a specific image in full resolution and send it to a colleague. Start, pause, or stop experiments, or capture an image to identify the best time for starting your experiment remotely.

Your Advantages

ipad screenshot - connect anywhere

Connect to your experiment anywhere at any time

Connect to your acquisition station via web client or mobile device and monitor the course of your experiment, review the whole experiment or open any image series available in the experiment tree.

ipad screenshot of imaging conditions

See imaging conditions at a glance

Review the channel settings defined for your experiment such as exposure time, gain, and filter cubes or take a look at environmental conditions like temperature, CO2 or O2 concentration (requires LAS X Environmental Control module).

ipad screenshot of chat window

Chat and send messages to colleagues

Multiple remote users can be connected with the system at the same time. Chat with connected colleagues, send emails to other colleagues or send messages to the user at the acquisition station.

Easy to use, Workflow-focused interface and functionality

Easy Operation Mode

  • Workflow oriented design for maximum ease of use, enabling even novices to become productive quickly
  • Same software driving confocal, widefield and super-resolution systems - greatly reducing training time.
  • Configure objectives, filter cubes and teach in parfocality and parcentricity within LAS X. No need to exit the imaging software for reconfiguration and fine tuning.
  • Save customized user settings and recall them during start up. User settings include all acquisition parameters, user specific configuration settings and layout of interface.
  • Full control of the microscope hardware while intelligent automation streamlines user interaction.
  • All system parameters can be reloaded and applied to future experiments for maximal reproducibility.
  • On widefield systems you can switch to the Easy Operation Mode and design your own layout of the user interface. Display the menus where you need them.
  • In the "Dye Assistant" confocal users can select between different acquisition configurations proposed by the system. Just enter the dyes of your sample and the confocal system will offer a set of possible system configurations along with their expected yield and cross talk.

Tested Reliability

  • No data loss, even after power blackout
  • Perfect synchronization between hardware and software
  • Precise timing for high-speed experiments
  • Stay in focus with the Adaptive Focus Control (AFC)
  • Run long term experiments over several days

Zebrafish Embryo
Courtesy of Lionel Newton, EMBL Heidelberg
Green: Lateral line primordium (GFP)
Red: Neuronal marker (DsRed)
Blue: Nucleus marker (BFP)
Gray: Muscles (SHG)

Zebrafish Embryo
Courtesy of Darren Gilmour, EMBL Heidelberg
Green: Mechanosensory lateral line placode (GFP)
Red: Innervating axons (DsRedExpress)

Drosophila Adult
Courtesy of Yuya Ohhara, Satoru Kobayashi, National Institute for Basic Biology, Japan
Green: Neuroendocrine cells (GFP)
Violet: Actin filaments (Phalloidin)

Modules and Applications

included optional not available
LAS X Modules and Functions Short Description Widefield Confocal
LAS X Core Basic building block for all LAS X systems. Operates without the need for a dongle.
Multi Channel Acquisition Allows the definition of up to 8 sequential camera channels per experiment. Confocal Scanning allows acqusition of a multitude of channels simultaneously or sequentially.
Time-Lapse Define the duration and frequency of image capture for time-lapse experiments.
Z-Control and Software Autofocus Position focus or capture 3 dimensional data. Includes software autofocus.
Mark and Find Define multiple stage locations and revisit them as part of a time-lapse or Z stacking experiment.
Stitching Create overview images of large fields of view.
Driver Non-Leica Cameras Allows the control of any third party camera supported by LAS X.
Lambda Scan Imaging method that acquires the emission spectrum in each image pixel using spectral detectors.
Lambda-lambda scan Imaging method that acquires the full excitation-emission spectrum in each image pixel using a tunable laser and spectral detectors. Allows Lambda square fluorescence mapping.
Dye Finder Multi-color restoration, channel unmixing (online/offline)
Dye Assistant Confocal users can select different dyes and LAS X sets up all hardware components accordingly.
LightGate Restriction of detection to a certain time window removes unwanted signal from autofluorescene or backscattering with HyD detectors.
Z intensitiy compensation Tool to compensate within a z-stack drop of fluorescence intensity that occurs deeper inside your sample with laser power and/or detector gain
Live Data Mode Define acquisition macros. Allows interactive data recording, logging of comments, job-sequencing, and online evaluation.
HCS A Versatile tool set for high content screening and automated microscopy.
3D Visualization Graphic card based processing for smooth and fast motion of 3D volumes in real time.
3D Analysis Easy and Versatile Wizard for 3D multi channel analysis and classification. Includes measurements in 3D and 3D process tools.
2D Analysis Easy and Versatile Wizard for 2D multi channel analysis and classification.
Measurements Module for 2D measurements such as distances, areas, angles. Count objects and classify objects manually. Create reports.
Calcium Imaging Module for online ratio measurement, online display of ratio graphs and ratio image.
Colocalization Histogram based colocalization and area measurements.
3D Deconvolution Fully integrated deconvolution algorithm using adaptive calculated or supplied PSF.
2D Deconvolution Algorithms for no neighbor and nearest neighbor deconvolution.
Data exchange between LAS X and Huygens Convenient exchange of Confocal, STED and MP images between LAS X LAS X and Huygens and Huygens Deconvolution Software.
FRET SE Powerful wizard for FRET acquisition and analysis (sensitized emission FRET).
FRET AB Powerful wizard for FRET acquisition and analysis (acceptor bleaching FRET).
FRAP Wizard for user friendly setup and analysis of FRAP and FLIP experiments, optional fast zoom in during bleach with resonant scanner.
FLIM Wizard for fast and reliable setup of FLI M experiments and full control of FLIM data aquisition.
FCS Wizard for fast and reliable setup of FCS and FLCS experiments and full control of F(L)CS data aquisition.
Electrophysiology Package to guide you through E-Phys experiments, incl. Automated recordings, interactive data acquisition and trigger functions.
SmartSTED Wizard Intuitive workflow to operate STED 3X with three simple sliders to define the general level of resolution increase and the amount of super-resolution in 3D. With a third slider to adjust between signal-to-noise and the number of achievable images.
CARS Calculator Automatic calculation of specific Raman wavenumbers according to the pumplaser wavelength used for exaction and vice versa.
CARS Spectral Scan Automatic spectral scan covering the Raman wavenumbers range 1200-3400cm-1
Environmental Control Have full control of your experimental conditions. Log the environmental data and monitor it during the experiment.
Mobile Connection Connect to your acquisition station via web client or mobile device and see the course of your experiment, review the whole experiment or open any image series available in the project tree.
Leica LAS X Core Leica LAS X 3D Analysis Leica LAS X 3D Visualization
Leica LAS X Core The Powerful Core Module for Life Science Research

The LAS X Core Module has full image viewing capabilities, including annotations, image overlay and image comparison. Create Maximum Intensity...

Leica LAS X 3D Analysis 3D Interactive Measurements and Wizard for Automatic 3D Analysis

With the software module Leica Application Suite (LAS X) 3D Analysis you can measure various aspects of intracellular structures such as volumes of...

Leica LAS X 3D Visualization True 3D Real-time Rendering with GPU-based Processing

The Leica Application Suite (LAS X) software module visualizes samples in three dimensions. It offers a wide variety of brilliant real-time 3D...

Supported Hardware

Supported Hardware (Widefield systems) Short description
CCD cameras, monochrome Leica: DFC365 FX, Leica DFC345 FX, DFC3000 G
Hamamatsu: ORCA-R2
Photometrics: CoolSNAP MYO, CoolSNAP KINO
CCD cameras, color Leica: DFC310 FX, DFC295, DFC495, DFC450 C, DFC450, DFC550
EM CCD cameras Hamamatsu: 9100-13, 9100-14
Andor: iXon3 897, iXon Ultra 897
Photometrics: Evolve 512, Evolve 512 Delta
Qimaging: Rolera-XR
sCMOS cameras, monochrome Hamamatsu: ORCA-Flash4.0 V2
Andor: Neo, Zyla 4.2
PCO: pco.edge 5.5
CMOS cameras, color Leica: DMC2900
LED fluorescence light sources for inverted research microscopes         

Lumencor Spectra X
Lumen Dynamics XLED1

LED fluorescence light sources for upright research microscopes            Leica SFL7000
LED light sources for stereo microscopes Leica LED5000 RL Ringlight
Leica LED5000 SLI Spotlight
Leica LED5000 MCI Multi contrast illumination system
Other fluorescence light sources Leica EL6000
Lumen Dynamics: X-Cite 200 DC
Motorized and scanning stages for inverted microscopes Leica motorized 3-plate-stage
Leica scanning stage 127x83
Prior ProScanTMII (114 x 76) scanning stage
Marzhauser SCANplus IM 130 x 85 scanning stage with encoder
LMT200 high precision scanning stage with encoder
Motorized and scanning stages for upright research microscopes Scanning stage 100 x 100 mot
Scanning stage 130 x 85 for 4 slides
Scanning stage for 8 slides
Motorized Stage 75 x 50 with integrated controller
Motorized Stage for DM5-6000 B (STAGE-BM mot. 7238)
Motorized and scanning stages for stereo microscopes LMT260 XY high precision scanning stage
Motorized IsoPro 6x4” XY stage
Incubators, Environmental equipment PeconTempController 2000-1
Pecon TempController 2000-2
Pecon CO2-Controller 2000
Pecon CO2/O2-Controller 2000
Tokai Hit INUBG2ATFP-WSKM Controller (110-120V)
Tokai Hit INUBG2ETFP-WSKM Controller (220-240V)
Life Imaging Services (Ludin) Cube2
Life Imaging Services (Ludin) Brick
Fine focus Leica SuperZ Galvo
Leica External Filter Wheels High speed filter wheels including (27ms switching time)
Dual View Beam Splitter Photometrics Dual View DV2
Leica Water Immersion Micro Dispenser Water immersion dispenser to compensate evaporation. Ideal for screening and multi positioning experiments (compatible objectives)
Leica MotCorr Motorized movement of correction ring (dedicated objectives only)

Microscope stands

Inverted microscopes Leica DMi8
Leica DMI6000 B
Leica DMI4000 B
Leica DMI3000 B
Leica DM IL LED
Upright research microscopes Leica DM6000 B
Leica DM5500 B
Leica DM5000 B
Leica DM4000 B LED
Upright routine microscopes Leica DM3000, Leica DM3000 LED
Leica DM2500
Leica DM2000, Leica DM2000 LED
Leica DM1000, Leica DM1000 LED
Fixed stage microscopes Leica DM6000 FS
Stereo microscopes and macroscopes Leica M165 FC
Leica M205 FA
Leica MacroFluo (Z16 APO A, Z6 APO A)

Supported Systems (Confocal)

TCS SP8
TCS SP8 SMD
TCS SP8 STED 3X
TCS SP8 X
TCS SP8 MP
TCS SP8 CARS
TCS SPE
TCS LSI

Downloads

Download the LAS X Mobile Connection app free of charge

Contact Our Software Experts

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