The Leica Application Suite X (LAS X) combines the most powerful features available today in microscope software into one package, focusing on usability in every aspect of the interface, functionality and workflow.
The workflow oriented design and the possibility to recall user defined system settings makes LAS X a very intuitive imaging platform customized to your needs. Combining innovative software and hardware options like the Mobile Connection module, the LightGate and Lambda Square Mapping on White Light Laser Systems or the water immersion micro dispenser makes LAS X an indispensable solution for confocal, widefield and super-resolution microscope systems.
Obtain reproducible analysis results rapidly and easily with the 3D and 2D image analysis wizards. Go step by step through the guided workflow: from applying filters, thresholding, and binary image processing to measurements and classification. Use a binary reference mask in multi-channel image analysis, for example, to count the number of spots in each nucleus. In addition, you can perform 2D tracking experiments or call ImageJ macros from within the 2D analysis wizard.
Save the analysis results with the experiment or create a report including histogram and binary masks and export it to Excel for further analysis.
For repetitive tasks, user-defined protocols can be saved and applied to new data sets. To manage large amounts of data, several data sets can be added to a batch list and processed at once. Results from different images can also be accumulated, providing statistical information.
Create reproducible results step-by-step using the Analysis workflow. At each step a set of analysis tools are available. The viewer provides immediate feedback on the applied settings. Additionally, tools such as auto threshold, split objects, or edge removal can be used for automated analysis of multiple samples. When needed, you can set pauses during an automated analysis to allow you to make fine adjustments, for example for binary image editing.
Get to the data you need with the flexible and interactive measurement table. Select objects in the image viewer and the corresponding measurement is highlighted in the results table, or vice versa. Choose from a large number of measurement parameters and rearrange or sort the columns as you like. Export measurements and statistics to Excel or save them with the experiment. Reproduce measurements easily with user defined protocols.
With multi-channel analysis, even complicated fluorescent experiments can be evaluated with ease. Create separate analysis channels for each fluorescent marker and assign an individual workflow to each. Then simply run an analysis of all aspects of your sample at the same time.
Combine analyses between separate channels to get object specific data. For example, apply a binary reference mask to count the number of spots in each nucleus.
Alternatively, apply multiple analysis channels to the same image. On a stained color image you can count the healthy cells in one analysis channel and create a second analysis channel to count the abnormal cells, for example.
Get unbiased and reproducible classification results even in situations where thresholding or filtering is not suitable. Use the intelligent classifier to group your results and display them in a histogram. The classifier can be trained with a training data set, then saved and applied to new data sets. The new objects will be divided into your predefined classes with a support vector machine.
Perform 2D tracking within the 2D Analysis wizard. Display particle trajectories, follow the objects along their trajectories over time and export the tracking results to excel.
Have full control of your experimental conditions with the LAS X Environmental Control module. Monitor and log the environmental data during an experiment. Control all environmental conditions within one interface and run temperature profiles, for example for heat shock experiments.
All environmental data is saved with the experiment and can be reviewed subsequently. Know when changes in temperature, CO2 or O2 conditions occur that might affect your experimental results.
In combination with LAS X Live Data Mode, macros can be generated to run temperature profiles. Acquisition can be set to start once a predefined temperature is reached. During the waiting time, a message displays the difference between nominal and actual environmental parameters. Alternatively, you can define that acquisition commences during ramping up of the temperature.
All environmental parameters are set within one interface. Activate range control to receive a warning when the upper or lower limits are exceeded. If needed, the experiment can be programmed to stop automatically once a limit is passed.
Don´t miss anything! With the LAS X Mobile Connection module you can stay connected to your experiment anywhere at any time. Display the imaging conditions on your remote device and review your experiment or download a specific image in full resolution and send it to a colleague. Start, pause, or stop experiments, or capture an image to identify the best time for starting your experiment remotely.
Connect to your acquisition station via web client or mobile device and monitor the course of your experiment, review the whole experiment or open any image series available in the experiment tree.
Review the channel settings defined for your experiment such as exposure time, gain, and filter cubes or take a look at environmental conditions like temperature, CO2 or O2 concentration (requires LAS X Environmental Control module).
Multiple remote users can be connected with the system at the same time. Chat with connected colleagues, send emails to other colleagues or send messages to the user at the acquisition station.
Courtesy of Lionel Newton, EMBL Heidelberg
Green: Lateral line primordium (GFP)
Red: Neuronal marker (DsRed)
Blue: Nucleus marker (BFP)
Gray: Muscles (SHG)
Courtesy of Darren Gilmour, EMBL Heidelberg
Green: Mechanosensory lateral line placode (GFP)
Red: Innervating axons (DsRedExpress)
Courtesy of Yuya Ohhara, Satoru Kobayashi, National Institute for Basic Biology, Japan
Green: Neuroendocrine cells (GFP)
Violet: Actin filaments (Phalloidin)
|LAS X Modules and Functions||Short Description||Widefield||Confocal|
|LAS X Core||Basic building block for all LAS X systems. Operates without the need for a dongle.|
|Multi Channel Acquisition||Allows the definition of up to 8 sequential camera channels per experiment. Confocal Scanning allows acqusition of a multitude of channels simultaneously or sequentially.|
|Time-Lapse||Define the duration and frequency of image capture for time-lapse experiments.|
|Z-Control and Software Autofocus||Position focus or capture 3 dimensional data. Includes software autofocus.|
|Mark and Find||Define multiple stage locations and revisit them as part of a time-lapse or Z stacking experiment.|
|Stitching||Create overview images of large fields of view.|
|Driver Non-Leica Cameras||Allows the control of any third party camera supported by LAS X.|
|Lambda Scan||Imaging method that acquires the emission spectrum in each image pixel using spectral detectors.|
|Lambda-lambda scan||Imaging method that acquires the full excitation-emission spectrum in each image pixel using a tunable laser and spectral detectors. Allows Lambda square fluorescence mapping.|
|Dye Finder||Multi-color restoration, channel unmixing (online/offline)|
|Dye Assistant||Confocal users can select different dyes and LAS X sets up all hardware components accordingly.|
|LightGate||Restriction of detection to a certain time window removes unwanted signal from autofluorescene or backscattering with HyD detectors.|
|Z intensitiy compensation||Tool to compensate within a z-stack drop of fluorescence intensity that occurs deeper inside your sample with laser power and/or detector gain|
|Live Data Mode||Define acquisition macros. Allows interactive data recording, logging of comments, job-sequencing, and online evaluation.|
|HCS A||Versatile tool set for high content screening and automated microscopy.|
|3D Visualization||Graphic card based processing for smooth and fast motion of 3D volumes in real time.|
|3D Analysis||Easy and Versatile Wizard for 3D multi channel analysis and classification. Includes measurements in 3D and 3D process tools.|
|2D Analysis||Easy and Versatile Wizard for 2D multi channel analysis and classification.|
|Measurements||Module for 2D measurements such as distances, areas, angles. Count objects and classify objects manually. Create reports.|
|Calcium Imaging||Module for online ratio measurement, online display of ratio graphs and ratio image.|
|Colocalization||Histogram based colocalization and area measurements.|
|3D Deconvolution||Fully integrated deconvolution algorithm using adaptive calculated or supplied PSF.|
|2D Deconvolution||Algorithms for no neighbor and nearest neighbor deconvolution.|
|Data exchange between LAS X and Huygens||Convenient exchange of Confocal, STED and MP images between LAS X and Huygens Deconvolution Software.|
|FRET SE||Powerful wizard for FRET acquisition and analysis (sensitized emission FRET).|
|FRET AB||Powerful wizard for FRET acquisition and analysis (acceptor bleaching FRET).|
|FRAP||Wizard for user friendly setup and analysis of FRAP and FLIP experiments, optional fast zoom in during bleach with resonant scanner.|
|FLIM||Wizard for fast and reliable setup of FLI M experiments and full control of FLIM data aquisition.|
|FCS||Wizard for fast and reliable setup of FCS and FLCS experiments and full control of F(L)CS data aquisition.|
|Electrophysiology||Package to guide you through E-Phys experiments, incl. Automated recordings, interactive data acquisition and trigger functions.|
|SmartSTED Wizard||Intuitive workflow to operate STED 3X with three simple sliders to define the general level of resolution increase and the amount of super-resolution in 3D. With a third slider to adjust between signal-to-noise and the number of achievable images.|
|CARS Calculator||Automatic calculation of specific Raman wavenumbers according to the pumplaser wavelength used for exaction and vice versa.|
|CARS Spectral Scan||Automatic spectral scan covering the Raman wavenumbers range 1200-3400cm-1|
|Environmental Control||Have full control of your experimental conditions. Log the environmental data and monitor it during the experiment.|
|Mobile Connection||Connect to your acquisition station via web client or mobile device and see the course of your experiment, review the whole experiment or open any image series available in the project tree.|
|Supported Hardware (Widefield systems)||Short description|
|CCD cameras, monochrome||
Leica: DFC365 FX, Leica DFC345 FX, DFC3000 G
Photometrics: CoolSNAP MYO, CoolSNAP KINO
|CCD cameras, color||Leica: DFC310 FX, DFC295, DFC495, DFC450 C, DFC450, DFC550|
|EM CCD cameras||
Hamamatsu: 9100-13, 9100-14
Andor: iXon3 897, iXon Ultra 897
Photometrics: Evolve 512, Evolve 512 Delta
|sCMOS cameras, monochrome||
Hamamatsu: ORCA-Flash4.0 V2
Andor: Neo, Zyla 4.2
PCO: pco.edge 5.5
|CMOS cameras, color||Leica: DMC2900|
|LED fluorescence light sources for inverted research microscopes||
Lumencor Spectra X
|LED fluorescence light sources for upright research microscopes||Leica SFL7000|
|LED light sources for stereo microscopes||
Leica LED5000 RL Ringlight
Leica LED5000 SLI Spotlight
Leica LED5000 MCI Multi contrast illumination system
|Other fluorescence light sources||
Lumen Dynamics: X-Cite 200 DC
|Motorized and scanning stages for inverted microscopes||
Leica motorized 3-plate-stage
Leica scanning stage 127x83
Prior ProScanTMII (114 x 76) scanning stage
Marzhauser SCANplus IM 130 x 85 scanning stage with encoder
LMT200 high precision scanning stage with encoder
|Motorized and scanning stages for upright research microscopes||
Scanning stage 100 x 100 mot
Scanning stage 130 x 85 for 4 slides
Scanning stage for 8 slides
Motorized Stage 75 x 50 with integrated controller
Motorized Stage for DM5-6000 B (STAGE-BM mot. 7238)
|Motorized and scanning stages for stereo microscopes||
LMT260 XY high precision scanning stage
Motorized IsoPro 6x4” XY stage
|Incubators, Environmental equipment||
Pecon TempController 2000-2
Pecon CO2-Controller 2000
Pecon CO2/O2-Controller 2000
Tokai Hit INUBG2ATFP-WSKM Controller (110-120V)
Tokai Hit INUBG2ETFP-WSKM Controller (220-240V)
Life Imaging Services (Ludin) Cube2
Life Imaging Services (Ludin) Brick
|Fine focus||Leica SuperZ Galvo|
|Leica External Filter Wheels||High speed filter wheels including (27ms switching time)|
|Dual View Beam Splitter||Photometrics Dual View DV2|
|Leica Water Immersion Micro Dispenser||Water immersion dispenser to compensate evaporation. Ideal for screening and multi positioning experiments (compatible objectives)|
|Leica MotCorr||Motorized movement of correction ring (dedicated objectives only)|
Leica DMI6000 B
Leica DMI4000 B
Leica DMI3000 B
Leica DM IL LED
|Upright research microscopes||
Leica DM6000 B
Leica DM5500 B
Leica DM5000 B
Leica DM4000 B LED
|Upright routine microscopes||
Leica DM3000, Leica DM3000 LED
Leica DM2000, Leica DM2000 LED
Leica DM1000, Leica DM1000 LED
|Fixed stage microscopes||Leica DM6000 FS|
|Stereo microscopes and macroscopes||
Leica M165 FC
Leica M205 FA
Leica MacroFluo (Z16 APO A, Z6 APO A)
|TCS SP8 SMD|
|TCS SP8 STED 3X|
|TCS SP8 X|
|TCS SP8 MP|
|TCS SP8 CARS|