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Water Flea Daphnia imaged by Electron Microscopy. Courtesy of Mag. Dr. Gruber, University of Vienna, Austria

Streamline your EM Sample Preparation Workflow for Biological Applications

Master EM sample preparation, including ultramicrotomy, for life sciences in this expert eBook!

Exploring the Structure and Life Cycle of Viruses

The SARS-CoV-2 outbreak started in late December 2019 and has since reached a global pandemic, leading to a worldwide battle against COVID-19. The ever-evolving electron microscopy methods offer a…

Advancing Cell Biology with Cryo-Correlative Microscopy

Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…

Workflows and Instrumentation for Cryo-electron Microscopy

Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…
Roland A. Fleck

Expert Knowledge on High Pressure Freezing and Freeze Fracturing in the Cryo SEM Workflow

Get an insight in the working methods of the laboratory and learn about the advantages of Cryo SEM investigation in EM Sample Preparation. Find out how high pressure freezing, freeze fracturing and…

Immersion Freezing for Cryo-Transmission Electron Microscopy: Applications

A well established usage case for cryo-TEM is three-dimensional reconstruction of isolated macromolecules, virus particles, or filaments. On one hand, these approaches are based on averaging of…

Immersion Freezing for Cryo-Transmission Electron Microscopy: Fundamentals

The high vacuum required in a transmission electron microscope (TEM) greatly impairs the ability to study specimens naturally occurring in an aqueous phase: exposing "wet" specimens to a pressure…

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