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TIRF Infinity Módulo DMi8 S
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Studying Actin Cytoskeleton and Microfilament Assembly
This article demonstrates how actin microfilament assemblies can be clearly visualized with superior resolution at the plasma membrane of a cell using a DMi8 S Infinity TIRF High Power imaging…
Plant Cell Development and Morphogenesis
The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence (TIRF) microscopy to better understand tubulin…
Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex
Plants, Bacteria, and Fungi possess a rigid cell wall that protects the cell and gives it shape. Animal cells, such as mammalian cells, have no outer wall, which exposes their plasma membrane to the…
Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy
Total Internal Reflection Fluorescence (TIRF) Microscopy is a technique that only illuminates dye molecules near a surface. In this video, the pioneer of TIRF Microscopy describes what this technique…
Universal PAINT – Dynamic Super-Resolution Microscopy
Super-resolution microscopy techniques have revolutionized biology for the last ten years. With their help cellular components can now be visualized at the size of a protein. Nevertheless, imaging…
Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips
The widefield super-resolution technique GSDIM (Ground State Depletion followed by individual molecule return) is a localization microscopy technique that is capable of resolving details as small as…
Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results
The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…
Total Internal Reflection Fluorescence (TIRF) Microscopy
Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…
Super-Resolution GSDIM Microscopy
The nanoscopic technique GSDIM (ground state depletion microscopy followed by individual molecule return) provides a detailed image of the spatial arrangement of proteins and other biomolecules within…
Campos de aplicação
Imagens de células vivas
Alterando a perspectiva de componentes únicos de um microscópio a uma solução de imagens de células vivas completamente em funcionamento, a Leica Microsystems integra o microscópio, o software de…
Fotomanipulação
O termo fotomanipulação engloba uma faixa de técnicas que utilizam as propriedades de moléculas fluorescentes para iniciar eventos e observar como complexos dinâmicos comportam-se com o passar do…
Microscópios de Superresolução STED e GSDIM (dSTORM)
A Leica Microsystems é pioneira na microscopia de superresolução trabalhando em estreita colaboração com grandes cientistas neste campo. A Leica Microsystems é a única fornecedora tanto de tecnologia…
Biologia celular
Quando a sua pesquisa é focada na compreensão da base celular da saúde e das doenças humanas, é crucial investigar as células de interesse em detalhes espaço-temporais e moleculares. Consequentemente,…
Virology
Soluções para aquisição de imagens e preparação de amostras para pesquisas em virologia
Técnicas avançadas de microscopia
As técnicas avançadas de microscopia abrangem tanto técnicas de imagem de alta resolução quanto de super-resolução. Estas técnicas são usadas principalmente para visualizar eventos biológicos em…
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