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Studying Actin Cytoskeleton and Microfilament Assembly

This article demonstrates how actin microfilament assemblies can be clearly visualized with superior resolution at the plasma membrane of a cell using a DMi8 S Infinity TIRF High Power imaging…
Studying Actin Cytoskeleton and Microfilament Assembly

Plant Cell Development and Morphogenesis

The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence (TIRF) microscopy to better understand tubulin…
Plant Cell Development and Morphogenesis

Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex

Plants, Bacteria, and Fungi possess a rigid cell wall that protects the cell and gives it shape. Animal cells, such as mammalian cells, have no outer wall, which exposes their plasma membrane to the…
Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex

Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy

Total Internal Reflection Fluorescence (TIRF) Microscopy is a technique that only illuminates dye molecules near a surface. In this video, the pioneer of TIRF Microscopy describes what this technique…
Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy

Universal PAINT – Dynamic Super-Resolution Microscopy

Super-resolution microscopy techniques have revolutionized biology for the last ten years. With their help cellular components can now be visualized at the size of a protein. Nevertheless, imaging…
Universal PAINT – Dynamic Super-Resolution Microscopy

Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips

The widefield super-resolution technique GSDIM (Ground State Depletion followed by individual molecule return) is a localization microscopy technique that is capable of resolving details as small as…
Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips

Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results

The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…
Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results

Total Internal Reflection Fluorescence (TIRF) Microscopy

Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…
Total Internal Reflection Fluorescence (TIRF) Microscopy

Super-Resolution GSDIM Microscopy

The nanoscopic technique GSDIM (ground state depletion microscopy followed by individual molecule return) provides a detailed image of the spatial arrangement of proteins and other biomolecules within…
Super-Resolution GSDIM Microscopy

TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells

Application of TIRF microscopy (Total Internal Reflection Fluorescence) allows the visualization of structures at the apical surface of polarized epithelial cells that have been hidden in conventional…
TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells

Exploring Cell Logistics

Using TIRF microscopy, scientists have been able to take a closer look at intracellular transport processes with the example of the galactose-binding protein Galectin-3, which has been identified as a…
Exploring Cell Logistics

Campos de aplicação

Imagens de células vivas

Alterando a perspectiva de componentes únicos de um microscópio a uma solução de imagens de células vivas completamente em funcionamento, a Leica Microsystems integra o microscópio, o software de…

Fotomanipulação

O termo fotomanipulação engloba uma faixa de técnicas que utilizam as propriedades de moléculas fluorescentes para iniciar eventos e observar como complexos dinâmicos comportam-se com o passar do…

Microscópios de Superresolução STED e GSDIM (dSTORM)

A Leica Microsystems é pioneira na microscopia de superresolução trabalhando em estreita colaboração com grandes cientistas neste campo. A Leica Microsystems é a única fornecedora tanto de tecnologia…

Biologia celular

Quando a sua pesquisa é focada na compreensão da base celular da saúde e das doenças humanas, é crucial investigar as células de interesse em detalhes espaço-temporais e moleculares. Consequentemente,…

Virology

Soluções para aquisição de imagens e preparação de amostras para pesquisas em virologia

Técnicas avançadas de microscopia

As técnicas avançadas de microscopia abrangem tanto técnicas de imagem de alta resolução quanto de super-resolução. Estas técnicas são usadas principalmente para visualizar eventos biológicos em…
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