Leica LMD6 e LMD7 Microdissecação a laser

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets
Mardamshina M, Dorka N, Thielert M, Bjoerklund F, Navarro FB, Casals AM, Kovacs F, Fox JEM, Horvath P, Macdonald PE, Mann M, Lundberg
bioRxiv 27 April 2025

Mass Spectrometry-Based Proteomics for Next-Generation Precision Oncology
Lin KT, Muneer G, Huang PR, Chen CS, Chen YJ
Mass Spectrom Rev. 2025 Apr 23. doi: 10.1002/mas.21932.

Deep Visual Proteomics maps proteotoxicity in a genetic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S, Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Metousis A, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Fabián O, Fraňková S, Krag A, Strnad P, Mann M
Nature. 2025 Apr 16. doi: 10.1038/s41586-025-08885-4.

DNA Research

The earliest stages of neoplastic transformation in Familial Adenomatous Polyposis
Stratton M, Leung SY, Wang Y, Robinson P, Thomas L, West H, Sampson J, Jung H, Fitzgerald S, Lee-Six H, Coorens T, Olafsson S, Lawson A, Hooks Y, Allen L, Roberts K, Yuen ST, Yan H, Foo CC, Lee B, Chan A, Chan A, Tsui W, Giger O, Martincorena I, Campbell
Research Square, 22. May 2025

Profiling (placental) DNA methylation in cell-free DNA across gestation: the Rotterdam Periconception Cohort
van Vliet MM, Boers RG, Boers JB, Schäffers OJM, van der Meeren LE, Gribnau J, Schoenmakers S, Steegers-Theunissen RPM
Mol Hum Reprod. 2025 May 8:gaaf011. doi: 10.1093/molehr/gaaf011.

Clinicopathological and molecular characterization of KRAS wild-type pancreatic ductal adenocarcinomas reveals precursor lesions with oncogenic mutations and fusions in RAS pathway genes
Toriyama K, Masago K, Shibata N, Haneda M, Kuwahara T, Natsume S, Kobayashi S, Fujita Y, Sasaki E, Yamao K, Kawashima H, Shimizu Y, Hara K, Yatabe Y, Hosoda W
J Pathol. 2025 May 2. doi: 10.1002/path.6432.

RNA Research

Osteocyte-derived extracellular vesicles mediate the bone-to-cartilage crosstalk and promote osteoarthritis progression
Liu N, Ma Y, Gong W, Shao X, Shi T, Li L, Wu W, Chen X, Shi Y, Zhang P, Lin J, Wang C, Fang D, Yang L, Wang P, Gao W, He Y, An X, Du R, Chen Y, Liu B, Qin J, Chen D, Cai P, Jiang Q, Guo B
Nat Commun. 2025 May 22;16(1):4746. doi: 10.1038/s41467-025-59861-5.

Emergence of the sex difference in calbindin D-28K neurons and the role of calbindin D-28K in clustering neurons of the preoptic area in mice
Koiso R, Kushida C, Kanaya M, Tsukahara S
Cell Tissue Res. 2025 May 21. doi: 10.1007/s00441-025-03981-3.

Novel Photoreceptor-Specific Promoters for Gene Therapy in Mid-to-Late Stage Retinal Degeneration
Sudharsan R, Murgiano L, Ahuja A, Sato Y, Kwok J, Dolgova N, Savina S, Sedorovitz M, Dufour VL, Aguirre GD, Byrne LC, Beltran WA
Mol Ther. 2025 May 21:S1525-0016(25)00390-9. doi: 10.1016/j.ymthe.2025.05.020.

Microglial TMEM119 binds to amyloid-β to promote its clearance in an Aβ-depositing mouse model of Alzheimer's disease
Liu J, Wang Z, Liang W, Zhang Z, Deng Y, Chen X, Hou Z, Xie Y, Wang Q, Li Y, Bai C, Li D, Mo F, Wang H, Wang D, Yuan J, Wang Y, Teng ZQ, Hu B
Immunity. 2025 May 9:S1074-7613(25)00181-5. doi: 10.1016/j.immuni.2025.04.018.

Proteomics, Lipidomics, Metabolomics

Integrative Spatial Proteomics and Single-Cell RNA Sequencing Unveil Molecular Complexity in Rheumatoid Arthritis for Novel Therapeutic Targeting
Wang X, Wang F, Iyer AS, Knight H, Duggan LJ, Yang Y, Jin L, Cui B, He Y, Schejbal J, Philips LA, Harvey BP, Sisó S, and Tian Y
Proteomes 2025, 13(2), 17; doi.org/10.3390/proteomes13020017

Spatial Proteomic Profiling Reveals Increased Levels of Cholesterol Synthesis Proteins in Psoriasis Vulgaris
Møller LBP, Kromann B, Kabatnik S, Hjortlund JH, Haulrig MB, Sølberg JBK, Bzorek M, Clark RA, Skov L, Mann M, Løvendorf MB, Dyring-Andersen 
J Invest Dermatol. 2025 May 22:S0022-202X(25)00489-0. doi: 10.1016/j.jid.2025.05.002.

Improved MALDI-MS Imaging of Polar and 2H-Labeled Metabolites in Mouse Organ Tissues
Kasarla SS, Fecke A, Smith KW, Flocke V, Flögel U, Phapale P
chemRxiv, May 2025

All-at-once spatial proteome profiling of complex tissue context with single-cell-type resolution by proximity proteomics
Mao Y, Li Y, Zheng Z, Xu Y, Ke M, He A, Liang F, Zhang K, Wang X, Gao W, Tian R
Cell Syst. 2025 May 5:101291. doi: 10.1016/j.cels.2025.101291.

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Highlight 2025: 

Rosenberger FA, et al.
Nature (2025 Apr 16)
https://www.nature.com/articles/s41586-025-08885-4

Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2

Highlights 2024: 

Method of the Year 2024: spatial proteomics. 
Nat Methods 21, 2195–2196 (2024). 
https://doi.org/10.1038/s41592-024-02565-3
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0

Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Fluorescence Applications

Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets
Mardamshina M, Dorka N, Thielert M, Bjoerklund F, Navarro FB, Casals AM, Kovacs F, Fox JEM, Horvath P, Macdonald PE, Mann M, Lundberg
bioRxiv 27 April 2025

Deep Visual Proteomics maps proteotoxicity in a genetic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S, Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Metousis A, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Fabián O, Fraňková S, Krag A, Strnad P, Mann M
Nature. 2025 Apr 16. doi: 10.1038/s41586-025-08885-4.

Single cell spatial proteomics maps human liver zonation patterns and their vulnerability to fibrosis
Weiss CAM, Brown LA, Miranda L, Pelizzoni P, Ben-Moshe S, Steigerwald S, Remmert K, Hernandez J, Borgwardt K, Rosenberger FA, Porat-Shliom N, Mann M
bioRxiv, 13. April 2025

Cancer Research

The earliest stages of neoplastic transformation in Familial Adenomatous Polyposis
Stratton M, Leung SY, Wang Y, Robinson P, Thomas L, West H, Sampson J, Jung H, Fitzgerald S, Lee-Six H, Coorens T, Olafsson S, Lawson A, Hooks Y, Allen L, Roberts K, Yuen ST, Yan H, Foo CC, Lee B, Chan A, Chan A, Tsui W, Giger O, Martincorena I, Campbell
Research Square, 22. May 2025

Clinicopathological and molecular characterization of KRAS wild-type pancreatic ductal adenocarcinomas reveals precursor lesions with oncogenic mutations and fusions in RAS pathway genes
Toriyama K, Masago K, Shibata N, Haneda M, Kuwahara T, Natsume S, Kobayashi S, Fujita Y, Sasaki E, Yamao K, Kawashima H, Shimizu Y, Hara K, Yatabe Y, Hosoda W
J Pathol. 2025 May 2. doi: 10.1002/path.6432.

AI-powered deep visual proteomics (DVP) for early pancreatic cancer insights 
Min J, Schweizer L, Zonderland G, Selvanesan BC, Oldenburg L, Hollingsworth MA, Grandgenett PM, Ummat I, Strauss MT, Mund A, Maitra A
Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 753.

Neurosciences

Emergence of the sex difference in calbindin D-28K neurons and the role of calbindin D-28K in clustering neurons of the preoptic area in mice
Koiso R, Kushida C, Kanaya M, Tsukahara S
Cell Tissue Res. 2025 May 21. doi: 10.1007/s00441-025-03981-3.

Single-cell genotyping and transcriptomic profiling of mosaic focal cortical dysplasia
Baldassari S, Klingler E, Teijeiro LG, Doladilhe M, Raoux C, Roig-Puiggros S, Bizzotto S, Couturier J, Gilbert A, Sami L, Ribierre T, Aronica E, Adle-Biassette H, Chipaux M, Jabaudon D, Baulac S
Nat Neurosci. 2025 Apr 30. doi: 10.1038/s41593-025-01936-z.

Neural tube defects induce abnormal astrocyte development by activation and epigenetic permissiveness of STAT3
Janik K, Jin LQ, Kyzy KZ, Kaminski R, Smith GM, Krynska B
Exp Neurol. 2025 Mar 30;389:115231. doi: 10.1016/j.expneurol.2025.115231.

Clinical/Pathology/Immunology Research Applications

Integrative Spatial Proteomics and Single-Cell RNA Sequencing Unveil Molecular Complexity in Rheumatoid Arthritis for Novel Therapeutic Targeting
Wang X, Wang F, Iyer AS, Knight H, Duggan LJ, Yang Y, Jin L, Cui B, He Y, Schejbal J, Philips LA, Harvey BP, Sisó S, and Tian Y
Proteomes 2025, 13(2), 17; doi.org/10.3390/proteomes13020017

Spatial Proteomic Profiling Reveals Increased Levels of Cholesterol Synthesis Proteins in Psoriasis Vulgaris
Møller LBP, Kromann B, Kabatnik S, Hjortlund JH, Haulrig MB, Sølberg JBK, Bzorek M, Clark RA, Skov L, Mann M, Løvendorf MB, Dyring-Andersen
J Invest Dermatol. 2025 May 22:S0022-202X(25)00489-0. doi: 10.1016/j.jid.2025.05.002.

Osteocyte-derived extracellular vesicles mediate the bone-to-cartilage crosstalk and promote osteoarthritis progression
Liu N, Ma Y, Gong W, Shao X, Shi T, Li L, Wu W, Chen X, Shi Y, Zhang P, Lin J, Wang C, Fang D, Yang L, Wang P, Gao W, He Y, An X, Du R, Chen Y, Liu B, Qin J, Chen D, Cai P, Jiang Q, Guo B
Nat Commun. 2025 May 22;16(1):4746. doi: 10.1038/s41467-025-59861-5.

Novel Photoreceptor-Specific Promoters for Gene Therapy in Mid-to-Late Stage Retinal Degeneration
Sudharsan R, Murgiano L, Ahuja A, Sato Y, Kwok J, Dolgova N, Savina S, Sedorovitz M, Dufour VL, Aguirre GD, Byrne LC, Beltran WA
Mol Ther. 2025 May 21:S1525-0016(25)00390-9. doi: 10.1016/j.ymthe.2025.05.020.0

Microglial TMEM119 binds to amyloid-β to promote its clearance in an Aβ-depositing mouse model of Alzheimer's disease
Liu J, Wang Z, Liang W, Zhang Z, Deng Y, Chen X, Hou Z, Xie Y, Wang Q, Li Y, Bai C, Li D, Mo F, Wang H, Wang D, Yuan J, Wang Y, Teng ZQ, Hu B
Immunity. 2025 May 9:S1074-7613(25)00181-5. doi: 10.1016/j.immuni.2025.04.018.

Developmental Research, Epigenetics & Aging

Profiling (placental) DNA methylation in cell-free DNA across gestation: the Rotterdam Periconception Cohort
van Vliet MM, Boers RG, Boers JB, Schäffers OJM, van der Meeren LE, Gribnau J, Schoenmakers S, Steegers-Theunissen RPM
Mol Hum Reprod. 2025 May 8:gaaf011. doi: 10.1093/molehr/gaaf011

Transcriptome Meta-Analysis Uncovers Cell-Specific Regulatory Relationships in Embryonic, Juvenile, Adult, and Aged Mouse Lens Epithelium and Fibers
Duot M, Coomson SY, Shrestha SK, Nagulla MVMK, Audic Y, Barve RA, Huang H, Gautier-Courteille C, Paillard L, Lachke SA
Invest Ophthalmol Vis Sci. 2025 Apr 1;66(4):42. doi: 10.1167/iovs.66.4.42.

Integrin α11β1 as a Key Collagen Receptor in Human Skin Dermis: Insight into Fibroblast Function and Skin Dermal Aging
Quan T, Qin Z, He T, Fisher GJ
J Invest Dermatol. 2025 Mar 24:S0022-202X(25)00364-1. doi: 10.1016/j.jid.2025.03.006

Plant Research

Laser microdissection and fluorescence in situ hybridization reveal the tissue-specific gene expression in the ovules of P. tabulaeformis Carr
Zhang X, Chen B, Song X, Wang Y, Zheng C, Gong Z
J Plant Physiol. 2025 Apr 22:309:154500. doi: 10.1016/j.jplph.2025.154500.

Role of an endodermis-specificmiR858b-MYB1Lmodule inthe regulation of Taxol biosynthesis inTaxus mairei
Yu C, Zhang D, Zhang L, Fang Z, Zhang Y, Lin W, Ma R, Zheng M, Bai E, Shen
The plant journal, Volume122, Issue1, 05 April 2025, e70135

Microbial-type terpene synthases significantly contribute to the terpene profile of glandular trichomes of the fern Dryopteris fragrans (L.)
Chen L, Gao R, Wei G, Luo S, Köllner TG, Xu H, Jiang Y, Zhang C, Chen X, Dickschat JS, Gershenzon J, Li S, Chang Y, Chen F
Plant J. 2025 Mar;121(6):e70079. doi: 10.1111/tpj.70079.

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Miscellaneous

Inhibiting 5-HT2C Receptor in the Hippocampus Rescues EphB2-Dependent Memory Impairment in Mice
Jana S, Chandran SK, Rosenblum K, Lamprecht R
European Journal of Pharmacology, doi.org/10.1016/j.ejphar.2025.177729.

Advances in Spatial Multi-Omics: A Review of Multi-Modal Mass Spectrometry Imaging and Laser Capture Microdissection-LCMS Integration
Lukowski JK, Cho BK, Calderon AZ, Dianati B, Stumpo K, Snyder S, Goo YA
Proteomics. 2025 May 12:e202400378. doi: 10.1002/pmic.202400378.

All-at-once spatial proteome profiling of complex tissue context with single-cell-type resolution by proximity proteomics
Mao Y, Li Y, Zheng Z, Xu Y, Ke M, He A, Liang F, Zhang K, Wang X, Gao W, Tian R
Cell Syst. 2025 May 5:101291. doi: 10.1016/j.cels.2025.101291.

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Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23

Live Cell Culture

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416