Leica LMD6 e LMD7 Microdissecação a laser
Perfeição de dissecação
This list presents current publications in the major application fields for laser microdissection and is updated monthly.
Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.
The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.
Download the complete list of LMD publications in "Downloads"
Single Cell Applications
Maternal smoking in early pregnancy disrupts placental function through syncytiotrophoblast and macrophage dysregulation
Valdes DS, Nimo J, Nonn, O, Ulrich J, Forstner D, Haider S, Ressler M, Pignitter M, Muller DN, Dechend R, Gauster M, Coscia F, Herse F
bioRxiv, July 15, 2025.
Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Kenny HA, Krishnan R, Kelliher L, Bilecz AJ, Heide J, Donle L, Shimizu A, Metousis A, Mendoza R, Nordmann TM, Rauch S, Richter S, Yan L, Rosenberger FA, Strauss MT, Kurnit KC, Thielert M, Rodriguez E, Müller-Reif JB, Yamada SD, Theis FJ, Mund A, Lastra RR, Mann M, and Lengyei E
Cancer Cell, 26 June 2025
Protocol for spatial proteomic profiling of tonsil cancer microenvironments using multiplexed imaging-powered deep visual proteomics
Zheng X, Mund A, Mann M
STAR Protoc. 2025 Jun 18;6(3):103901. doi: 10.1016/j.xpro.2025.103901.
DNA Research
Genomic characterization of malignant progression in neoplastic pancreatic cysts
Noë M, Niknafs N, Fischer CG, Hackeng WM, Beleva Guthrie V, Hosoda W, Debeljak M, Papp E, Adleff V, White JR, Luchini C, Pea A, Scarpa A, Butturini G, Zamboni G, Castelli P, Hong SM, Yachida S, Hiraoka N, Gill AJ, Samra JS, Offerhaus GJA, Hoorens A, Verheij J, Jansen C, Adsay NV, Jiang W, Winter J, Albores-Saavedra J, Terris B, Thompson ED, Roberts NJ, Hruban RH, Karchin R, Scharpf RB, Brosens LAA, Velculescu VE, Wood LD
Nat Commun. 2020 Aug 14;11(1):4085. doi: 10.1038/s41467-020-17917-8.
Mutation profile and chromosomal abnormality in adenomyosis
Suda K, Takahashi K, Tamura R, Saito K, Yamaguchi M, Yachida N, Adachi S, Kase H, Okuda S, Yoshihara K, and Nakaoka H
Reproduction, 07 Jul 2025, REP-25-0132
Two-hit events occurred independently in bilateral breast cancers in a germline double heterozygous carrier for BRCA1 and BRCA2
Semba R, Eguchi H, Takatsu M, Hashizume T, Moteki H, Maeno K, Murakami F, Watanabe J, Kutomi G, Arai M
Breast Cancer. 2025 Jul 2. doi: 10.1007/s12282-025-01740-4.
RNA Research
Polysaccharide distribution and cell wall deposition in the endosperm and outer layers of developing sorghum grains
Costes C, Le Bot L, Alvarado C, Daniel S, Sanz SN, Calatayud C, Soriano A, Guillon F, Mameri H, Terrier N, Saulnier L, Francin-Allami M
Journal of Cereal Science, doi.org/10.1016/j.jcs.2025.104239.
Gene expression landscape of the Brassica napus seed reveals subgenome bias in both space and time.
Ziegler DJ, Khan D, Kalichuk JL, Becker MG, Pasha A, Provart NJ, Belmonte MF
Plant Physiol. 2025 Jun 28:kiaf283. doi: 10.1093/plphys/kiaf283.
Attenuated immune surveillance during squamous cell transformation of pancreatic adenosquamous cancer defines new therapeutic opportunity for cancer interception
Chen X, Sun S, Li S, Yu S, Chen J, Chen X
J Immunother Cancer. 2025 Jun 23;13(6):e012066. doi: 10.1136/jitc-2025-012066.
Proteomics, Lipidomics, Metabolomics
A perspective on integrating digital pathology, proteomics, clinical data and AI analytics in cancer research.
Guedes J, Woldmar N, Szasz AM, Wieslander E, Pawłowski K, Horvatovich P, Malm J, Szadai L, Németh IB, Marko-Varga G, Gil J
J Proteomics. 2025 Jul 16;320:105493. doi: 10.1016/j.jprot.2025.105493.
Maternal smoking in early pregnancy disrupts placental function through syncytiotrophoblast and macrophage dysregulation
Valdes DS, Nimo J, Nonn, O, Ulrich J, Forstner D, Haider S, Ressler M, Pignitter M, Muller DN, Dechend R, Gauster M, Coscia F, Herse F
bioRxiv, July 15, 2025.
Tumor necrosis facilitates perihilar cholangiocarcinoma metastasis by ANGPTL6-augmented vessel permeability and tumor dissemination
Zhang H, Chen W, Li X, Song Z, Liu Y, Liu S, Su Z, Wu W, Chen H, Qin C, Lin S, Zhang F, Lu X, Tang Q, Yu X, Xu L, Liu C
JHEP Reports, doi.org/10.1016/j.jhepr.2025.101514.
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Highlight 2025:
Rosenberger FA, et al.
Nature (2025 Apr 16)
https://www.nature.com/articles/s41586-025-08885-4
Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2
Highlights 2024:
Method of the Year 2024: spatial proteomics.
Nat Methods 21, 2195–2196 (2024).
https://doi.org/10.1038/s41592-024-02565-3
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0
Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409
Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8
Fluorescence Applications
Maternal smoking in early pregnancy disrupts placental function through syncytiotrophoblast and macrophage dysregulation
Valdes DS, Nimo J, Nonn, O, Ulrich J, Forstner D, Haider S, Ressler M, Pignitter M, Muller DN, Dechend R, Gauster M, Coscia F, Herse F
bioRxiv, July 15, 2025.
Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Kenny HA, Krishnan R, Kelliher L, Bilecz AJ, Heide J, Donle L, Shimizu A, Metousis A, Mendoza R, Nordmann TM, Rauch S, Richter S, Yan L, Rosenberger FA, Strauss MT, Kurnit KC, Thielert M, Rodriguez E, Müller-Reif JB, Yamada SD, Theis FJ, Mund A, Lastra RR, Mann M, and Lengyei E
Cancer Cell, 26 June 2025
Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets
Mardamshina M, Dorka N, Thielert M, Bjoerklund F, Navarro FB, Casals AM, Kovacs F, Fox JEM, Horvath P, Macdonald PE, Mann M, Lundberg
bioRxiv 27 April 2025
Cancer Research
Genomic characterization of malignant progression in neoplastic pancreatic cysts
Noë M, Niknafs N, Fischer CG, Hackeng WM, Beleva Guthrie V, Hosoda W, Debeljak M, Papp E, Adleff V, White JR, Luchini C, Pea A, Scarpa A, Butturini G, Zamboni G, Castelli P, Hong SM, Yachida S, Hiraoka N, Gill AJ, Samra JS, Offerhaus GJA, Hoorens A, Verheij J, Jansen C, Adsay NV, Jiang W, Winter J, Albores-Saavedra J, Terris B, Thompson ED, Roberts NJ, Hruban RH, Karchin R, Scharpf RB, Brosens LAA, Velculescu VE, Wood LD
Nat Commun. 2020 Aug 14;11(1):4085. doi: 10.1038/s41467-020-17917-8.
A perspective on integrating digital pathology, proteomics, clinical data and AI analytics in cancer research.
Guedes J, Woldmar N, Szasz AM, Wieslander E, Pawłowski K, Horvatovich P, Malm J, Szadai L, Németh IB, Marko-Varga G, Gil J
J Proteomics. 2025 Jul 16;320:105493. doi: 10.1016/j.jprot.2025.105493.
Tumor necrosis facilitates perihilar cholangiocarcinoma metastasis by ANGPTL6-augmented vessel permeability and tumor dissemination
Zhang H, Chen W, Li X, Song Z, Liu Y, Liu S, Su Z, Wu W, Chen H, Qin C, Lin S, Zhang F, Lu X, Tang Q, Yu X, Xu L, Liu C
JHEP Reports, doi.org/10.1016/j.jhepr.2025.101514.
Neurosciences
Mechanotransduction in migrating cortical interneurons during neurogenesis
van Delft P
thesis, 2025
Sex- and subregion-specific vulnerability of the hippocampus and amygdala to intermittent hypoxia in relation to learning/memory function and anxiety tendencies of infant rats
Takenouchi Y, Hosomichi J, Angkanawaraphan K, Maeda H, Hong H, Changsiripun C, Ono T
Sleep Breath. 2025 Jun 9;29(3):209. doi: 10.1007/s11325-025-03382-4.
Emergence of the sex difference in calbindin D-28K neurons and the role of calbindin D-28K in clustering neurons of the preoptic area in mice
Koiso R, Kushida C, Kanaya M, Tsukahara S
Cell Tissue Res. 2025 May 21. doi: 10.1007/s00441-025-03981-3.
Clinical/Pathology/Immunology Research Applications
Oncostatin M Drives Th2 Polarized Allergic Airway Inflammation Through Fibroblast Reprogramming and Endoplasmic Reticulum Stress
Zhang H, Chen X, Liu L Zheng H, Yang X, Yin K, Yao Q, Li L & Yang P
International Journal of Nanomedicine, 14 Jul 2025, 9019-9030, DOI: 10.2147/IJN.S535265
Lesion penetration predicts tuberculosis regimen success: a validated translational tool for de-risking late-stage drug development
Savic R, Ernest J, Bustion A, Patel A, Sarathy J, Kaya F, Rodriguez S, Strydom N, Silva C, Flori L, Vu H-A, Westergren V, Via L, Dartois V
Research Square, 25th June 2025
Laser Microdissection and Mass Spectrometry Reveals Diverse Membranous Nephropathy Antigens
Inácio A, Domingues P, Piedade A, Mendes B, Fonseca F, Vrana J, Theis J, Fervenza FC, Soto K, Sethi S
Kidney International Reports, 23 June 2025
Developmental Research, Epigenetics & Aging
Maternal smoking in early pregnancy disrupts placental function through syncytiotrophoblast and macrophage dysregulation
Valdes DS, Nimo J, Nonn, O, Ulrich J, Forstner D, Haider S, Ressler M, Pignitter M, Muller DN, Dechend R, Gauster M, Coscia F, Herse F
bioRxiv, July 15, 2025.
Polysaccharide distribution and cell wall deposition in the endosperm and outer layers of developing sorghum grains
Costes C, Le Bot L, Alvarado C, Daniel S, Sanz SN, Calatayud C, Soriano A, Guillon F, Mameri H, Terrier N, Saulnier L, Francin-Allami M
Journal of Cereal Science, doi.org/10.1016/j.jcs.2025.104239.
Age-dependent accumulation of mitochondrial tRNA mutations in mouse kidneys linked to mitochondrial kidney diseases
Zhang L, Xu Z, Jing J, Chai G, Xie G, Ru Y, Lv Q, Zuo X, Zhang Q, Chen J, Jin H, Liu N, Kong M, Shen B, Liu M, Jiang L, Wang X, Zhang Y, Jiang M
Nat Aging. 2025 Jun 27. doi: 10.1038/s43587-025-00909-y.
Plant Research
Polysaccharide distribution and cell wall deposition in the endosperm and outer layers of developing sorghum grains
Costes C, Le Bot L, Alvarado C, Daniel S, Sanz SN, Calatayud C, Soriano A, Guillon F, Mameri H, Terrier N, Saulnier L, Francin-Allami M
Journal of Cereal Science, doi.org/10.1016/j.jcs.2025.104239.
Gene expression landscape of the Brassica napus seed reveals subgenome bias in both space and time.
Ziegler DJ, Khan D, Kalichuk JL, Becker MG, Pasha A, Provart NJ, Belmonte MF
Plant Physiol. 2025 Jun 28:kiaf283. doi: 10.1093/plphys/kiaf283.
RsLBD3 regulates the secondary growth of taproot by integrating auxin and cytokinin signaling in radish (Raphanus sativus L.)
Dong J, Wang Y, Xu L, Li B, Zhang X, Chen Y, Ying J, Chen S, Cui F, Liu L
J Integr Plant Biol. 2025 May 7. doi: 10.1111/jipb.13918.
Forensics
Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.
Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807
Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136
General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/
Miscellaneous
Methane-powered sea spiders: Diverse, epibiotic methanotrophs serve as a source of nutrition for deep-sea methane seep Sericosura
Dal Bó B, Guo Y, Mayr MJ, Pereira OS, Levin LA, Orphan VJ, Goffredi SK
Proc Natl Acad Sci U S A. 2025 Jul;122(26):e2501422122. doi: 10.1073/pnas.2501422122.
An ultrasensitive spatial tissue proteomics workflow exceeding 100 proteomes per day
Klingeberg M, Krisp C, Fritzsche S, Schallenberg S, Hornburg D, and Coscia F
bioRxiv, 05 June 2025
Micromorphological features of the structure of the tooth pulp chamber wall in dogs
Frolov VV, Egunova AV, Ivantsov VA
International Journal of Veterinary Medicine. 2025;(1):222-227. (In Russ.)
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Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23
Live Cell Culture
A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX
A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023
SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416
