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STELLARIS 5 & STELLARIS 8 Plataforma de microscopia confocal

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Spheroid stained with Cyan: Dapi nuclear countertain; Green AF488 Involucrin; Orange AF55 Phalloidin Actin; Magenta AF647 CK14.

Notable AI-based Solutions for Phenotypic Drug Screening

Learn about notable optical microscope solutions for phenotypic drug screening using 3D-cell culture, both planning and execution, from this free, on-demand webinar.
Murine esophageal organoids (DAPI, Integrin26-AF 488, SOX2-AF568) imaged with the THUNDER Imager 3D Cell Culture. Courtesy of Dr. F.T. Arroso Martins, Tamere University, Finland.

How to Get Deeper Insights into your Organoid and Spheroid Models

In this eBook, learn about key considerations for imaging 3D cultures, such as organoids and spheroids, and discover microscopy solutions to shed new insights into dynamic processes in 3D real-time
Fluorescence microscopy image of liver tissue where DNA in the nuclei are stained with Feulgen-pararosanilin and visualized with transmitted green light.

Epi-Illumination Fluorescence and Reflection-Contrast Microscopy

This article discusses the development of epi-illumination and reflection contrast for fluorescence microscopy concerning life-science applications. Much was done by the Ploem research group…
Application example of hyperspectral imaging

Potential of Multiplex Confocal Imaging for Cancer Research and Immunology

Explore the new frontiers of multi-color fluorescent imaging: from image acquisition to analysis
Molecular structure of the green fluorescent protein (GFP)

Introduction to Fluorescent Proteins

Overview of fluorescent proteins (FPs) from, red (RFP) to green (GFP) and blue (BFP), with a table showing their relevant spectral characteristics.
Multi-tissue array with 4 markers shown including DAPI, NaKATPase, PanCk, and Vimentin.

Spatial Biology: Learning the Landscape

Spatial Biology: Understanding the organization and interaction of molecules, cells, and tissues in their native spatial context
In vivo imaging of a mouse pial and cortical vasculature through a glass window (ROSAmT/mG::Pdgfb-CreERT2 mouse meningeal and cortical visualization following tamoxifen induction and craniotomy). Courtesy: Thomas Mathivet, PhD

Windows on Neurovascular Pathologies

Discover how innate immunity can sustain deleterious effects following neurovascular pathologies and the technological developments enabling longitudinal studies into these events.
Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green. Acquisition with one detector, intensity information shown in grey. The two markers can be separated using lifetime information: LifeAct-GFP (cyan), MitoTracker Green (magenta). Image acquired with STELLARIS 5.

The Power of Reproducibility, Collaboration and New Imaging Technologies

In this webinar you willl learn what impacts reproducibility in microscopy, what resources and initiatives there are to improve education and rigor and reproducibility in microscopy and how…
AI-based workflow for fast rare event detection in living biological samples using Autonomous Microscopy powered by Aivia

AI Microscopy Enables the Efficient Detection of Rare Events

Localization and selective imaging of rare events is key for the investigation of many processes in biological samples. Yet, due to time constraints and complexity, some experiments are not feasible…
How is microscopy used in spatial biology - Teaserimage

How is Microscopy Used in Spatial Biology? A Microscopy Guide

Different spatial biology methods in microscopy, such as multiplex imaging, are helping to better understand tissue landscapes. Learn more in this microscopy guide.

Virtual Reality Showcase for STELLARIS Confocal Microscopy Platform

Take a seat directly next to our experts in front of the microscope, configure various multicolor experiments and see immediately the result. Be part of the highly interactive discovery tour of the…
Five-color FLIM-STED

Five-color FLIM-STED with One Depletion Laser

Webinar on five-color STED with a single depletion laser and fluorescence lifetime phasor separation.

Confocal Imaging of Immune Cells in Tissue Samples

In this webinar, you will discover how to perform 10-color acquisition using a confocal microscope. The challenges of imaged-based approaches to identify skin immune cells. A new pipeline to assess…
Combining spectrally resolved detection and fluorescence lifetime multiplexing

Live-Cell Fluorescence Lifetime Multiplexing Using Organic Fluorophores

On-demand video: Imaging more subcellular targets by using fluorescence lifetime multiplexing combined with spectrally resolved detection.
Donor (D) and acceptor (A) molecule which participate in FRET (Förster resonance energy transfer).

What is FRET with FLIM (FLIM-FRET)?

This article explains the FLIM-FRET method which combines resonance energy transfer and fluorescence lifetime imaging to study protein-protein interactions.

Insights into Vesicle Trafficking

STELLARIS provides integral access to complementary layers of information for dynamic, structural, and mechanistic insights into vesicle trafficking.

Visualizing Protein-Protein Interactions by Non-Fitting and Easy FRET-FLIM Approaches

The Webinar with Dr. Sergi Padilla-Parra is about visualizing protein-protein interaction. He gives insight into non-fitting and easy FRET-FLIM approaches.
Spectral separation of 11 fluorophores coupled to polystyrene beads on a STELLARIS confocal system.

Multiplexing through Spectral Separation of 11 Colors

Fluorescence microscopy is a fundamental tool for life science research that has evolved and matured together with the development of multicolor labeling strategies in cells tissues and model…
Transverse histological cut of a rabbit tongue. 50 Mpixels images (2326 µm x 1739 µm) in 14 x 18 tiles. Lifetime gives an additional contrast that allows to differentiate different structures in histological stainings.

A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)

The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.

TauInteraction – Studying Molecular Interactions with TauSense

Fluorescence microscopy constitutes one of the pillars in life sciences and is a tool commonly used to unveil cellular structure and function. A key advantage of fluorescence microscopy resides in the…
Depth coding of endothelial cells in zebrafish eye. Courtesy of Basile Gurchenkov, Imaging Center of the IGBMC, Illkirch-Graffenstaden, France.

Gentle 3D Imaging of Biological Samples

For 3D imaging of biological samples, light sheet microscopy is a powerful and sensitive tool allowing you to measure (sub)cellular dynamics over long periods of time. Meanwhile, confocal microscopy,…
Identification of distinct structures_roundworm_Ascaris_female

Find Relevant Specimen Details from Overviews

Switch from searching image by image to seeing the full overview of samples quickly and identifying the important specimen details instantly with confocal microscopy. Use that knowledge to set up…
Confocal imaging of a SARS-CoV-2-infected epithelia at 4 dpi (orthogonal sections)

STELLARIS Contributes to Understanding COVID-19 Infection

What are the structural and functional consequences of a SARS-CoV-2 infection? To answer this important question, the authors used 3D cultures that mimic an airway epithelium and used different…
Dynamic Signal Enhancement powered by Aivia:  Truly simultaneous multicolor imaging of live cells (U2OS) in 3D

Artificial Intelligence and Confocal Microscopy – What You Need to Know

This list of frequently asked questions provides “hands-on” answers and is a supplement to the introductory article about Dynamic Signal Enhancement powered by Aivia "How Artificial Intelligence…
Dynamic Signal Enhancement powered by Aivia: Truly simultaneous multicolor imaging of live cells (U2OS) in 3D

How Artificial Intelligence Enhances Confocal Imaging

In this article, we show how artificial intelligence (AI) can enhance your imaging experiments. Namely, how Dynamic Signal Enhancement powered by Aivia improves image quality while capturing the…

Spectroscopic Evaluation of Red Blood Cells

Hemoglobinopathies are a major healthcare problem. This study presents a possible diagnostic tool for thalassemia which is based on confocal spectroscopy. This approach exploits spectral detection and…

Visualizing Protein Degradation and Aggregation in the Living Cell

Our guest speaker, Prof Dr Eric Reits, presents his work on neurodegenerative disorders. Reits’ group are experts on the subject of Huntington’s disease and work towards identifying leads for…

Life Beyond the Pixels: Deep Learning Methods for Single Cell Analysis

Our guest speaker Prof Dr Peter Horvath presents his work on single cell-based large-scale microscopy experiments. This novel targeting approach includes the use of machine learning models and…
Nematostella

Live Cell Imaging Gallery

Live cell microscopy techniques are fundamental to get a better understanding of cellular and molecular function. Today, widefield microscopy is the most common technique used to visualize cell…

Super-Resolution Microscopy Image Gallery

Due to the diffraction limit of light, traditional confocal microscopy cannot resolve structures below ~240 nm. Super-resolution microscopy techniques, such as STED, PALM or STORM or some…

Tissue Image Gallery

Visual analysis of animal and human tissues is critical to understand complex diseases such as cancer or neurodegeneration. From basic immunohistochemistry to intravital imaging, confocal microscopy…
Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imagerwith a 2x 0.15 NA objective at 3.4x zoomwas used to produce this 425 μm Z-stack (26 positions), which is presented here as an Extended Depth of Field(EDoF)projection.

Neuroscience Images

Neuroscience commonly uses microscopy to study the nervous system’s function and understand neurodegenerative diseases.

Multicolor Image Gallery

Fluorescence multicolor microscopy, which is one aspect of multiplex imaging, allows for the observation and analysis of multiple elements within the same sample – each tagged with a different…

Cancer Research Image Gallery

Fluorescence microscopy allows the study of changes occurring in tissue and cells during cancer development and progression. Techniques such as live cell imaging are critical to understand cancer…

Cell Biology Image Gallery

Cell biology studies the structure, function and behavior of cells, including cell metabolism, cell cycle, and cell signaling. Fluorescence microscopes are an integral part of a cell biologist…

Adding Dimensions to Multiplex Molecular Imaging

Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better understand the underlying cellular and molecular mechanisms of complex…
Root-hypocotyl junction of Arabidopsis thaliana. Image acquired with TauContrast. Sample courtesy: Dr. Melanie Krebs, COS, University of Heidelberg.

Benefits of TauContrast to Image Complex Samples

In this interview, Dr. Timo Zimmermann talks about his experience with the application of TauSense tools and their potential for the investigation of demanding samples such as thick samples or…

A New World of Confocal Applications with the Next Generation White Light Lasers

As biological questions get more complex, there is an increasing need to study multiple events simultaneously in the same specimen. When preparing the specimen for imaging experiments, this need is…
Influenca in lung epithelial cells (porcine) - THUNDER Imager 3D Cell Culture Influenca virus – red, cilia – green, Nuclei – blue.

How Can Immunofluorescence Aid Virology Research?

Modern virology research has become as crucial now as ever before due to the global COVID-19 pandemic. There are many powerful technologies and assays that virologists can apply to their research into…

Obtenha o máximo de informações de sua amostra com o LIGHTNING

O LIGHTNING é um processo adaptivo totalmente automático para a extração de informações que revela estruturas finas e detalhes que, de outra forma, simplesmente não seriam visíveis. Ao contrário de…
Multicolor 3D imaging of live mammalian cell.

Expanding the Frontiers of Confocal Live Cell Imaging

Here we explore how STELLARIS unlocks the full power and potential of live cell studies by overcoming many common limitations and fully integrating fluorescence lifetime-based information to add a new…

Explore Innovative Techniques to Separate Fluorophores with Overlapping Spectra

In this article we explore several strategies you can take to improve the separation of fluorophores and increase the number of fluorescent probes you can distinguish in your sample.

STELLARIS White Light Lasers

When it comes to choosing fluorescent probes for your multi-color experiments, you shouldn’t have to compromise. Now you can advance beyond conventional excitation sources that limit your fluorophore…

TauSense Technology Imaging Tools

Leica Microsystems’ TauSense technology is a set of imaging modes based on fluorescence lifetime. Found at the core of the STELLARIS confocal platform, it will revolutionize your imaging experiments.…

The Power HyD Detector Family

Powerful photon counting detectors on the STELLARIS confocal platform provide improved photon counting, ultra-sensitive imaging and more color options in the NIR spectrum.

How to Uncover Hidden Dimensions in Research with Lifetime Imaging

Learn how fluorescence lifetime imaging adds information depth to your confocal experiments and reveals novel insights that are difficult or impossible to discover using conventional intensity-based…
Fluorescence microscopy image on the left with no distinction between the fluorescent signal and background autofluorescence. FLIM was used in the image on the right to differentiate autofluorescence in chloroplasts (blue) from the desired fluorescent signal from the cell membrane (green).

Learn how to Remove Autofluorescence from your Confocal Images

Autofluorescence can significantly reduce what you can see in a confocal experiment. This article explores causes of autofluorescence as well as different ways to remove it, from simple media fixes to…

DIVE Multiphoton Microscope Image Gallery

Today’s life science research focusses on complex biological processes, such as the causes of cancer and other human diseases. A deep look into tissues and living specimens is vital to understanding…

Nobel Prize 2013 in Physiology or Medicine for Discoveries of the Machinery Regulating Vesicle Traffic

On October 7th 2013, The Nobel Assembly at Karolinska Institutet has decided to award The Nobel Prize in Physiology or Medicine 2012 jointly to James E. Rothman, Randy W. Schekman and Thomas C. Südhof…

Handbook of Optical Filters for Fluorescence Microscopy

Fluorescence microscopy and other light-based applications require optical filters that have demanding spectral and physical characteristics. Often, these characteristics are application-specific and…
Fluorescence microscope image of a life-science specimen

An Introduction to Fluorescence

This article gives an introduction to fluorescence and photoluminescence, which includes phosphorescence, explains the basic theory behind them, and how fluorescence is used for microscopy.

Campos de aplicação

Imagens de células vivas

Alterando a perspectiva de componentes únicos de um microscópio a uma solução de imagens de células vivas completamente em funcionamento, a Leica Microsystems integra o microscópio, o software de…

Microscópios de Superresolução STED e GSDIM (dSTORM)

A Leica Microsystems é pioneira na microscopia de superresolução trabalhando em estreita colaboração com grandes cientistas neste campo. A Leica Microsystems é a única fornecedora tanto de tecnologia…

Pesquisa de câncer

O câncer é uma doença complexa e heterogênea causada por células com deficiência na regulação do crescimento. Mudanças genéticas e epigenéticas em uma ou em um grupo de células prejudicam o…

Organoides e cultura celular 3D

Um dos recentes avanços mais impressionantes na pesquisa de ciências da vida é o desenvolvimento de sistemas de cultura celular 3D, como organoides, esferoides ou modelos órgão-em-chip. Uma cultura…

Neurocientífica

Você está trabalhando rumo a uma melhor compreensão de doenças neurodegenerativas ou estudando a função do sistema nervoso? Veja como você pode fazer descobertas com as soluções de aquisição de…

Microscopia de fluorescência

A fluorescência é um dos fenômenos físicos mais comumente usados na microscopia biológica e analítica, principalmente devido a sua alta sensibilidade e alta especificidade. A fluorescência é uma forma…

Fluorescence Lifetime Imaging

A microscopia de aquisição de imagens de tempo de vida de fluorescência (FLIM - Fluorescence lifetime imaging microscopy) é uma técnica de aquisição de imagens que utiliza as propriedades inerentes…

Biologia celular

Quando a sua pesquisa é focada na compreensão da base celular da saúde e das doenças humanas, é crucial investigar as células de interesse em detalhes espaço-temporais e moleculares. Consequentemente,…

Virology

Soluções para aquisição de imagens e preparação de amostras para pesquisas em virologia

Organismos modelo na pesquisa

Um organismo modelo é uma espécie usada pelos pesquisadores para estudar processos biológicos específicos. Eles têm características genéticas similares aos seres humanos e são usados tipicamente em…
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