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Studying Actin Cytoskeleton and Microfilament Assembly
This article demonstrates how actin microfilament assemblies can be clearly visualized with superior resolution at the plasma membrane of a cell using a DMi8 S Infinity TIRF High Power imaging…
Plant Cell Development and Morphogenesis
The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence (TIRF) microscopy to better understand tubulin…
Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex
Plants, Bacteria, and Fungi possess a rigid cell wall that protects the cell and gives it shape. Animal cells, such as mammalian cells, have no outer wall, which exposes their plasma membrane to the…
Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy
Total Internal Reflection Fluorescence (TIRF) Microscopy is a technique that only illuminates dye molecules near a surface. In this video, the pioneer of TIRF Microscopy describes what this technique…
Universal PAINT – Dynamic Super-Resolution Microscopy
Super-resolution microscopy techniques have revolutionized biology for the last ten years. With their help cellular components can now be visualized at the size of a protein. Nevertheless, imaging…
Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips
The widefield super-resolution technique GSDIM (Ground State Depletion followed by individual molecule return) is a localization microscopy technique that is capable of resolving details as small as…
Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results
The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…
Total Internal Reflection Fluorescence (TIRF) Microscopy
Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…
Super-Resolution GSDIM Microscopy
The nanoscopic technique GSDIM (ground state depletion microscopy followed by individual molecule return) provides a detailed image of the spatial arrangement of proteins and other biomolecules within…
TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells
Application of TIRF microscopy (Total Internal Reflection Fluorescence) allows the visualization of structures at the apical surface of polarized epithelial cells that have been hidden in conventional…
Exploring Cell Logistics
Using TIRF microscopy, scientists have been able to take a closer look at intracellular transport processes with the example of the galactose-binding protein Galectin-3, which has been identified as a…
Campos de aplicación
Imagen de célula viva
Con un cambio de perspectiva desde componentes individuales del microscopio a una solución de captura y procesamiento de imágenes de células vivas totalmente operativa, Leica Microsystems integra…
Fotomanipulación
El término fotomanipulación engloba una serie de técnicas que utilizan las propiedades de las moléculas fluorescentes para iniciar eventos y observar el comportamiento de compuestos dinámicos en…
Microscopios de súper-resolución y nanoscopios
Leica Microsystems es una empresa pionera en microscopía de superresolución, que trabaja en estrecha colaboración con científicos de primera línea en este campo. Leica Microsystems es el único…
Biología celular
Descubra cómo puede extraer el máximo rendimiento a sus investigaciones en materia de biología celular y amplíe sus descubrimientos con las soluciones de generación de imágenes de Leica Microsystems.
Virología
¿Su interés en investigación se centra en infecciones víricas y enfermedades? Descubra cómo puede adquirir conocimientos sobre virología con soluciones de generación de imágenes y preparación de…
Técnicas avanzadas de microscopía
Las técnicas de microscopía avanzada abarcan tanto las técnicas de imagen de alta resolución como las de superresolución. Estas técnicas se utilizan principalmente para visualizar eventos biológicos…