Ciências da vida
Ciências da vida
Este é o lugar para expandir seus conhecimentos, recursos de pesquisa e aplicações práticas de microscopia em vários campos científicos. Saiba como obter visualização precisa, interpretação de imagens e avanços na pesquisa. Encontre informações perspicazes sobre microscopia avançada, técnicas de geração de imagens, preparação de amostras e análise de imagens. Os tópicos abordados incluem biologia celular, neurociência e pesquisa do câncer, com foco em aplicações e inovações de ponta.
How Fluorescence Guides Sectioning of Resin-embedded EM Samples
Electron microscopes, including transmission electron microscopes (TEM) and scanning electron microscopes (SEM), are widely utilized to gain detailed structural information about biological samples or…
How to Save Time and Samples by Automated Ultramicrotomy
This article describes how 3D micro-CT data of a resin-embedded electron microscopy sample can be used to trim the specimen down to a defined target plane prior to sectioning. The interactive and…
How Marine Microorganism Analysis can be Improved with High-pressure Freezing
In this application example we showcase the use of EM-Sample preparation with high pressure freezing, freeze substiturion and ultramicrotomy for marine biology focusing on ultrastructural analysis of…
Advancing Cellular Ultrastructure Research
Freeze-fracture and freeze-etching are useful tools for studying flexible membrane-associated structures such as tight junctions or the enteric glycocalyx. Freeze-fracture and etching are two…
Targeting Active Recycling Nuclear Pore Complexes using Cryo Confocal Microscopy
In this article, how cryo light microscopy and, in particular cryo confocal microscopy, is used to improve the reliability of cryo EM workflows is described. The quality of the EM grids and samples is…
Essential Guide to Ultramicrotomy
When studying samples, to visualize their fine structure with nanometer scale resolution, most often electron microscopy is used. There are 2 types: scanning electron microscopy (SEM) which images the…
Soluções de revestimento por pulverização e fratura por congelamento
De revestimento a temperaturas ambiente e baixo vácuo a revestimento criogênico a alto vácuo, a Leica Microsystems abrange a gama completa de demandas para revestimentos.
High Resolution Array Tomography with Automated Serial Sectioning
The optimization of high resolution, 3-dimensional (3D), sub-cellular structure analysis with array tomography using an automated serial sectioning solution, achieving a high section density on the…
Visualization of DNA Molecules
Precise low angle rotary shadowing with heavy metals (like platinum) can be used in transmission electron microscopy (TEM) to observe molecular details of objects previously absorbed on a thin, low…
