Streamlined HPF for Cryo ET: From Waffle Method to Serial Li

Make high-pressure freezing workflows more robust, reproducible, and scalable—so you can reliably prepare thick and complex samples for cryo‑ET.

Preparing high-quality lamellae from thick biological specimens remains a major bottleneck in cryo‑ET workflows. While high-pressure freezing (HPF) enables vitrification beyond plunge-freezing limits, many labs still face fragile, time‑intensive workflows that limit routine use.

In this webinar, four experts from leading research institutions and industry share practical strategies to streamline HPF-enabled cryo‑ET—helping you improve reproducibility, reduce handling complexity, and increase throughput.

What you will learn

• Streamline sample preparation workflows - Learn how targeted technical refinements can make HPF-based cryo ET more robust and easier to implement in daily lab routines.
• Increase lamella yield from thick samples - Understand how the waffle method enables bulk vitrification and supports preparing multiple lamellae from challenging specimens.
• Reduce handling and complexity after freezing - Discover simplified HPF workflows—including Autogrid-compatible approaches—to minimize post freezing steps.
• Boost throughput with advanced lift out strategies - Explore Serial Lift Out methods to prepare multiple lamellae from a single transfer and expand contextual sampling.
• Establish a reliable starting point for cryo workflows - See how HPF supports consistent downstream cryo FIB milling and cryo ET analysis.

Agenda Overview

• Optimizing cryo ET workflows for HPF samples
• Autogrid-compatible HPF workflows
• Serial Lift Out for higher throughput
• Practical implementation of the Waffle Method

Speakers

Georg Wolff

Cryo Electron Tomography Specialist, EMBL Imaging Centre, Heidelberg

Christoph Kaiser

Postdoctoral Researcher, Max Planck Institute of Biochemistry

Miriam Sarah Weber

Postdoctoral Scientist, University of Zurich

Andreia Pinto

Manager Application Workflow Specialists, Leica Microsystems

Who should attend

• Cryo ET researchers working with complex or thick samples
• Core facility managers aiming to scale cryo workflows
• Scientists exploring HPF as an alternative to plunge freezing
• Users looking to improve lamella yield and workflow robustness

Register now to streamline your HPF workflows and make cryo ET more reproducible, scalable, and efficient.