Leica EM PACT2 Cryopréparation Préparation d’échantillons pour MET/MEB Produits Accueil Leica Leica Microsystems

Congélateur haute pression avec un système de transfert rapide Leica EM PACT2

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  • In vivo Reconstitution of Algal Triacylglycerol Production in Saccharomyces cerevisiae, Chun-Hsien Hung1, Kazue Kanehara1 and Yuki Nakamura1, 2*, 1 Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, 2 Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, Saitama, Japan, *Correspondence: Yuki Nakamura, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753380/

  • Cell Wall N-linked Mannoprotein Biosynthesis Requires Goa1p, a Putative Regulator of Mitochondrial Complex I in Candida albicans, Xiaodong She1,2, Richard Calderone1*, Michael Kruppa3, Douglas Lowman3, David Williams3, Lili Zhang2, Ying Gao2, Kasra Khamooshi1, Weida Liu2, Dongmei Li1, 1 Georgetown University Medical Center, Department of Microbiology & Immunology, Washington, DC, 20057, United States of America, 2 Institute of Dermatology, Chinese Academy of Medical Sciences (CAMS), Jiangsu Key laboratory of Molecular Biology for Skin Disease and STIs, Nanjing, 210029, China, 3 Department of Surgery, Department of Biomedical Sciences and Center of Excellence in Inflammation, Infectious Diseases, and Immunity, East Tennessee State University, Johnson City, Tennessee, 37614,United States of America, http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0147175

  • Flash-and-Freeze Electron Microscopy: Coupling Optogenetics with High-Pressure Freezing, Shigeki Watanabe, M. Wayne Davis, and Erik M. Jorgensen, Springer New York, Neuromethods, Volume 84, pp 43-57  2014

  • High-resolution secondary ion mass spectrometry analysis of carbon dynamics in mycorrhizas formed by an obligately myco-heterotrophic orchid, Jeremy Bougoure1, Martha Ludwig2, Mark Brundrett4, John Cliff3, Peta Clode3, Matt Kilburn3, & Pauline Grierson2, 1School of Earth and Environment , 2School of Plant Biology, 3Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia and 4Department of Environment and Conversation, Bentley Delivery Centre, Swan Region, PO Box 1167, Bentley, WA 6983, Australia; Plant, Cell & Environment, Volume 37, Issue 5, May 2014
  • A new approach to improve the quality of ultrathin cryo-sections; its use for immunogold EM and correlative electron cryo-tomography, Erik Bosa,b,d, Celso SantAnnab, Helmut Gnaegic, Roberta F. Pintob, Raimond B.G. Ravellid, Abraham J. Kosterd, Wanderley de Souzab, Peter J. Petersa,e,*, ELSEVIER, Journal of Structural Biology, Volume 175, Issue 1, July 2011

  •  Nanoscale changes in chromatin organization represent the initial steps of tumorigenesis: a transmission electron microscopy study, Lusik Cherkezyan1, Yolanda Stypula-Cyrus1, Hariharan Subramanian1, Craig White1, Mart Dela Cruz2, Ramesh K Wali2, Michael J Goldberg3, Laura K Bianchi3, Hemant K Roy2 and Vadim Backman, BMC Cancer, Cell and molecular biology, Volume 14, 2014

  • Visualizing  compaction of polysomes in bacteria, Nicolas Cougot1, Anne-Elisabeth Molza1, Jeremy Delesques1, Emmanuel Giudice1, Annie Cavalier1, Jean-Paul Rolland1, Gwennola Ermel2, Carlos Blanco2, Daniel Thomas1 and Reynald Gillet1,3*, ELSEVIER Journal of Molecular Biology, Volume 426, Issue 2, 2013

  • Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy, C.C. Chien a,b,, P.Y. Tsenga, H.H. Chena, T.E. Huaa, S.T. Chena, W.H. Lenga, C.H. Wanga, Y.Hwua,b,c,d,*, G.C. Yine, K.S. Liangf, F.R. Chenb, Y.S. Chug,H.I. Yehh, Y.C. Yangh, C.S. Yangh, G.L. Zhangj, J.H. Jek, G. Margaritondol, ELSEVIER, Biotechnology Advances, Volume 32, Issue 3, May-June 2013
  • A novel method for high-pressure freezing of adherent cells for frozen hydrated sectioning and CEMOVIS, R.J.Mesmann, Membrane Enzymology, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands, Biomolecular Imaging, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands, Elsevier, Journal of Structural Biology, Volume 183, Issue 3, September 2013, Pages 527-530                                      

  • Stacked Endoplasmic Reticulum Sheets Are Connected by Helicoidal Membrane Motifs, Mark Terasaki,1,9,* Tom Shemesh,3,9 Narayanan Kasthuri,4 Robin W. Klemm,5 Richard Schalek,4 Kenneth J. Hayworth,4,10, Arthur R. Hand,6 Maya Yankova,2 Greg Huber,1,7 Jeff W. Lichtman,4 Tom A. Rapoport,5,* and Michael M. Kozlov8,9 ,1Department of Cell Biology, 2Central Electron Microscopy Facility University of Connecticut Health Center, Farmington, CT 06030, USA, 3Department of Chemical Engineering, Columbia University, New York, NY 10027, USA, 4Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA, 5Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston,MA02115, USA, 6Departments of Craniofacial Sciences and Cell Biology, University of Connecticut School of Dental Medicine, Farmington, CT 06032, USA, 7Kavli Institute for Theoretical Physics, Kohn Hall, University of California, Santa Barbara, Santa Barbara, CA 93106-4030, USA, 8Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, 69978 Tel Aviv, Israel9These authors contributed equally to this work, 10Present address: Howard Hughes Medical Institute Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA, Cell, Volume 154, Issue 2 285-296, 18 July 2013 ,http://dx.doi.org/10.1016/j.cell.2013.06.031, http://www.cell.com/retrieve/pii/S0092867413007708?cc=y
  • Deep Sequencing of Myxilla (Ectyomyxilla) methanophila, an Epibiotic Sponge on Cold-Seep Tubeworms, Reveals Methylotrophic, Thiotrophic, and Putative Hydrocarbon-Degrading Microbial Associations , Shawn M. Arellano & On On Lee & Feras F. Lafi & Jiangke Yang & Yong Wang & Craig M. Young & Pei-Yuan Qian, SpringerLink, Microbiial Ecology, February 2013, Volume 65, Issue 2, pp450-461, http://link.springer.com/article/10.1007/s00248-012-0130-y#

  • Role of CEACAM1 and CEACAM20 in an In Vitro Model of Prostate Morphogenesis, Hui Zhang - City of Hope Irell & Manella Graduate School of Biological Sciences, Duarte, California, United States of America, Hui Zhang, John E. Shively - Department of Immunology, Beckman Research Institute of City of Hope, Duarte, California, United States of America, Andreas Eisenried - Anästhesiologische Klinik, Universitätsklinikum Erlangen, Erlangen, Germany, Wolfgang Zimmermann - Labor für Tumorimmunologie, LIFE-Zentrum, München, Germany, PLOS One, January 24, 2013, http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0053359

  • Immunohistochemical localization of caffeine in young Camellia sinensis (L.) O. Kuntze (tea) leaves, Shane V. van Breda1,2, Chris F. van der Merwe3, Hannes Robbertse4, Zeno Apostolides2, 2Department of Biochemistry, 3Laboratory for Microscopy and Microanalysis,4Department of Plant Production and Soil Science, University of Pretoria, Lynnwood Road,Hatfield, Pretoria, 0002, Gauteng, South Africa. SpingerLink, Planta, March 2013, Volume 237, Issue 3, pp 849-858

  • Fine Structure of hippocampal mossy fiber synapses following rapid high-pressure freezing,  Shanting Zhao, Daniel Studer, Werner Graber, Sigrun Nestel, Michael Frotscher,Department of Structural Neurobiology, Center for Molecular Neurobiology Hamburg (ZMNH), University of Hamburg, Germany, Institute of Anatomy, University of Bern, Switzerland,  Institute of Anatomy and Cell Biology, University of Freiburg, Germany, Wiley, Epilepsia, Volume 53, Issue Supplement s1, pages 4-8, June 2012,
  • Trichobilharzia regenti: Antigenic structures of intravertebrate stages,  Marta Chanová, Lucie Lichtenergová, Jana Bulatová, Libor Mikes and Petr Horák, Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University in Prague, 128 00 Prague, Czech Republic, Department of Parasitology, Faculty of Science, Charles University in Prague, 128 44 Prague, Czech Republic, Department of Microbiology, 3rd Faculty of Medicine, Charles University in Prague, 100 34 Prague, Czech Republic, Springer Ling, Central European Journal of Biology, Volume 7, Number 2 82012) 83 - 90   http://www.springerlink.com/content/g1885562q3j7517x/about/
  • Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early Caenorhabditis elegans Embryo,  Ina Woog, Silke White, Mandy Büchner, Martin Srayko and Thomas Müller-Reichert, Medical Thoretical Center, Medical Faculty Carl Gustav Carus, University of Technology Dresden, Department of biological Sciences, University of Alberta, Canada, Correlative light Electron Microscopy, Methods in Cell Biology, Volume 111

  • Light and electron microscopy observations of embryogenesis and egg development in the human liver fluke, Opisthorchis viverrini (Platyhelminthes, Digenea),Panita Khampoosa, Malcolm K. Jones, Erica M. Lovas, Tuanchai Srisawangwong, Thewarach Laha, Supawadee Piratae, Chalida Thammasiri, Apiporn Suwannatrai, Bungorn Sripanidkulchai and Veerachai Eursitthichai, et al., Springer Link, Parasitology Research, Volume 110, Number 2(2012), 799 - 808 
  • Insight into the ultrastructural organisation of sporulated oocysts of Eimeria nieschulzi (Coccidia, Apicomplexa), Eric Seemann, Thomas Kurth, Rolf Entzeroth, Institute of Zoology, TU Dresden, Germany, DFG-Center for Regenerative Therapies Dresden, TU Dresden, Germany, Institut für Biochemie, Jena, Germany, Springer Link, Parasitology Research, 2012, Volume 1/1928 - Volume 111/2012
  • Optimal preservation of the shark retina for ultrastructural analysis: An asessment of chemical microwave, and high-pressure freezing fixation techniques,Blake K. Harahush, Kathryn Green, Rick Webb, Nathan S. Hart, Shaun P. Collin, School of Biomedical Sciences, The University of Queensland, Brisbane,School of Animal Biology,The University of Western Australia, Western Australia, The Oceans Institute, The University of Western Australia, Centre of Microscopy and Microanalysis, University of Queensland,Brisbane, Australia, Wiley, Microscopy Research and Technique, Volume 75, Issue 9, Pages 1218 - 1228, September 2012                                                             http://onlinelibrary.wiley.com/doi/10.1002/jemt.22052/abstract?

  • Improvement of Ultrastructural Preservation of "Eimeria" Oocysts by Microwave-assisted Chemical Fixation or by High Pressure Freezing and Freeze Substitution  Thomas Kurth, Stefanie Wiedmer, Rolf Entzeroth, TU Dresden, DFG-Center forRegenerative Therapies Dresden, Germany, TU Dresden, Institute of Zoology, Dresden, Germany, Elsevier, Protist, Volume 163, Issue 2, March 2012, Pages 296-305                          http://www.sciencedirect.com/science/article/pii/S1434461011000484
  • Postlipolytic insulin-dependent remodeling of micro lipid droplets in adipocytes, Nicholas Ariottia,* Samantha Murphya,*,, Nicholas A. Hamiltona, Lizhen Wub, Kathryn Greenc, Nicole L. Schiebera, Peng Lib, Sally Martina,‡, and Robert G. Partona,c,1,aInstitute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia , bTsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China , cCentre for Microscopy and Microanalysis, University of Queensland, Brisbane, Queensland 4072, Australia, MBOC Molecular Biology of The Cell, doi. 10.1091/mbc.E11-10-0847, Volume 23, No 10 1826 – 1837, May 2012, http://www.molbiolcell.org/content/23/10/1826.long

  • A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperture embedding in LR White resin, Margarita Sobol, J Nebesářová and Pavel Hozák, Histochemistry and Cell Biology, Volume 135, Number 1, 103-110, 2011,
  • The power of correlative microscopy: multi-modal, multi-scale, multi-dimensional, Jeffrey Caplan1, Marc Niethammer2, Russell M Taylor II3, Kirk J Czymmek1,4,  1 Delaware Biotechnology Institute Bio-Imaging Center, University of Delaware, Newark, DE 19711, United States, 2 Biomedical Research Imaging Center (BRIC), School of Medicine, University of North Carolina, Chapel Hill, 27599, United States, 3 CISMM Resource, Department of Computer Science, University of North Carolina, Chapel Hill, NC 27599-3175, United States, 4 Department of Biological Sciences, University of Delaware, Newark, DE 19716, United States, Elsevier, Current Opinion in Structural Biology, Volume 21, Issue 5, October 2011, Pages 686 – 693, http://www.sciencedirect.com/science/article/pii/S0959440X11001035

  • Role of outer-membrane cytochromes MtrC and OmcA in the biomineralization of ferrihydrite by Shewanella oneidensis MR-1, C. L. REARDON1,, A. C. DOHNALKOVA1, P. NACHIMUTHU1, D. W. KENNEDY1, D. A. SAFFARINI2, B. W. AREY1, L. SHI1, Z. WANG1, D. MOORE1, J. S. MCLEAN1, D. MOYLES3, M. J. MARSHALL1, J. M. ZACHARA1, J. K. FREDRICKSON1, A. S. BELIAEV1, Geobiology, Volume 8, Issue 2, pages 56 - 68, January 2010 http://onlinelibrary.wiley.com/doi/10.1111/j.1472-4669.2009.00226.x/abstract    
  • A precise and rapid mapping protocol for correlative light and electron microscopy of small invertebrate organisms,Irina Kolotuev*1, Yannick Schwab† and Michel Labouesse*, *Department of Cell and Developmental Biology, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), 67400 Illkirch, France, and Imaging Center, CNRS/Inserm/Universite de Strasbourg (UdS), 1 rue Laurent Fries, BP10142, 67400 Illkirch, France, Biol.Cell (2010) 102, 121-132, http://www.ncbi.nlm.nih.gov/pubmed/19807690

  • A review of high-pressure freezing preparation techniques for correlative light and electron microscopy of the same cells and tissues. K.L. MCDonald, Journal of Microscopy, Volume 235, Issue 3, pages 273-281, September 2009, 

  • Moving EM: the Rapid Transfer System as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing.  P. Verkade, Journal of Microscopy, Volume 230, Issue 2, pages 317-328, May 2008,
  • STEM tomography for thick biological specimens, Kazuhiro Aoyama, Tomoko Takagi, Ai Hirase and Atsuo Miyazawa, FEI Company Japan Ltd., Application Laboratory, NSS-II Building, 2-13-34 Kohnan, Minato-ku, Tokyo 108-0075, Japan,Laboratory of Electron Microscopy, Japan Women's University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan,Bio-multisome Research Team, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan,CREST, JST, Japan, Ultramicroscopy, 2008 Dec;109 (1): 70-80, Epub 2008 Sep 10.
  • Electron microscopy of the early Caenorhabditis elegans embryo. T. Müller-Reichert, J. Mäntler, M. Srayko, E. O'Toole, Journal of Microscopy, Volume 230, Issue2, pages 297-307, May 2008,
  • High-pressure Freezing in CLEM, A Correlative Light Electron Microscopy Study, Paul Verkade Dr., Imaging & Microscopy, Volume 9, Issue 1, pages 49-51, March 2007,
  • Recent Advance in High-Pressure Freezing, Equipment- and Specimen-Loading Methods. Kent L. McDonald, Mary Morphew, Paul Verkade and Thomas Müller-Reichert, Electron Microscopy, Methods in Molecular Biology, 2007, Volume 369, 143 - 173
  • Mice Lacking the Nuclear Pore Complex Protein ALADIN Show Female Infertility but Fail To Develop a Phenotype Resembling Human Triple A Syndrome Angela Huebner, Philipp Mann, Elvira Rohde, Angela M. Kaindl, Martin Witt,Paul Verkade, Sibylle Jakubiczka, Mario Menschikowski, Gisela Stoltenburg-Didinger and Katrin Koehler, Molecular and Cellular Biology, March 2006, p. 1879-1887, Vol. 26, No. 5,
  • New comprehension of the apicoplast of Sarcocystis by transmission electron tomography Cveta Tomova*, Willie J.C. Geerts†, Thomas Mueller-Reichert‡, Rolf Entzeroth* and Bruno M. Humbel†1*Institut Zoologie/Spezielle Zoologie, Technische Universitaet Dresden, Helmholtzstrasse 10, D-01062 Dresden, Germany, †Electron Microscopy and Structural Analysis, Department of Biology, Faculty of Sciences, Utrecht University, Padualaan 8, NL-3584 CH, Utrecht, The Netherlands, and ‡Max Planck Institute of Molecular Cell Biology and Genetics, Electron Microscopy Facility,Pfotenhauerstrasse 108, D-01307 Dresden, Germany, Biology of the Cell (2006) 98, (535-545)