THUNDER Imager Live Cell & 3D Assay
Décodez la biologie 3D en temps réel*
Les THUNDER Imagers vous offrent une solution pour l'analyse 3D de cultures de cellules complexes, que vous souhaitiez étudier les cellules souches, les sphéroïdes ou les organoïdes.
Vos avantages :
- Haut débit pour de meilleures statistiques et un flux de travail efficace
- Performances d'imagerie supérieures grâce à un système simple d'utilisation
- Conditions physiologiques optimales pour des résultats significatifs
Les THUNDER Imagers intègrent « Computational Clearing », la technologie innovante de Leica. Elle élimine en toute efficacité le flou hors focus en temps réel, permettant ainsi d'utiliser judicieusement des échantillons 3D avec des microscopes à fluorescence avec une détection par caméra. La sensibilité élevée du système garantit une phototoxicité et un photoblanchiment faibles, en d'autres termes un plus haut débit avec des conditions optimales.
Haut débit pour de meilleures statistiques et un flux de travail efficace
Automatisez vos analyses 3D de cultures de cellules pour étudier efficacement les modèles de pathologies de demain. THUNDER vous permet d'imager des volumes d'échantillons importants, tels que des organoïdes pulmonaires, à grande vitesse. En outre, l'automatisation réduit au maximum l'interaction de l'utilisateur, même pour les expériences complexes.
Résultats :
- Données précises et fiables en moins de temps
- Plus haut débit
- Meilleures statistiques et meilleurs résultats
Conditions physiologiques optimales - exposition minimale à la lumière
En matière de culture cellulaire 3D, il est primordial d'observer la véritable physiologie pour obtenir des résultats significatifs. En règle générale, vous souhaitez étudier vos cellules pendant qu'elles se trouvent dans un état proche de l'état naturel en optimisant les conditions expérimentales, par exemple, l'intensité lumineuse la plus faible et les durées d'exposition les plus courtes possibles.
THUNDER Imager Live Cell répond à ces exigences avec une source LED haut de gamme, dotée d'une petite bande passante optimisée pour l'excitation. Même avec un faible éclairage et des durées d'exposition courtes, la caméra sCMOS sensible haut de gamme livre des informations d'image pertinentes grâce à une efficacité quantique de 82 % maximum.
Afin de réduire davantage l'exposition de l'échantillon à la lumière, l'éclairage est limité à la durée d'enregistrement réelle. L'obturateur de la caméra est synchronisé avec la source de lumière LED Lumencor à grande vitesse (durée de commutation de 20 µs) afin de réduire au maximum le photoblanchiment.
Reliable quantification of an entire mouse retina with THUNDER
Quantitative approaches to retina imaging analysis often emphasizes to provide comprehensive descriptions of the retina morphology and function. Retina abnormalities as well as translational clinical applications need a reliable workflow to reproduce the transgenic target screening. Therefore, repetitive imaging of the morphology requires a system solution with accurate results which can be constantly reproduced. With the THUNDER Imager 3D Assay you are able to clearly visualize the morphology and reliable count intracellular details, like single cell nuclei distribution in the retina.
With a THUNDER Imager 3D Assay you have these advantages
- Immediate blur removal that help you visualize more intracellular details
- More utilizable depth with widefield approach
- Reliable quantification
- Ready-to-use for your particular workflow analysis
![[Translate to french:] [Translate to chinese:] Control Swiss adult female mouse wholemount retina showing Iba1+ microglial cells (Alexa Fluor® 488 green-fluorochrome) and Brn3a+ retinal ganglion cells (Alexa Fluor® 594 red-fluorochrome). Image courtesy of Experimental Ophtalmology group, University of Murcia (Spain).](/fileadmin/global/products/THUNDER/THUNDER_Imager_3D_Assay_Retina.jpg "[Translate to french:] [Translate to chinese:] Mouse Retina")
Visualization of brain organoids with a THUNDER Imager Live Cell
Brain organoids as novel model systems are used to investigate human brain development and disorders. These self-assembled 3-dimensional cytoarchitectures are often characterized via multiple transgenic marker imaging. Classical challenges in these workflows are quantifying dynamics of molecules in time besides keeping physiological conditions and reaching sample depth at low signal levels. Therefore, the THUNDER Imager Live Cell is suitable to study the development of organoids close to physiological conditions, as our LED light sources help to minimize photobleaching. Furthermore, the timed detection of even though low protein signal levels is possible without the need to change the carrier.
With a THUNDER Imager Live Cell you have these advantages
- Possibility to observe your specimens in plastic bottom dishes makes your workflow more efficient
- Capacity to detect low signals of molecules with high QE camera
- Low bleaching and low sample perturbance with widefield approach and precise timed LED illumination
Low-phototoxicity in long-term live cell time-lapse imaging
Explant cell cultures are oftentimes difficult to conduct imaging experiments with, as they require a stable cell culture environment and low-phototoxic imaging conditions. This example of explant cell culture imaging from Dr. Laura Shankman at the University of Virginia shows how abdominal aorta cells were able to be stably imaged over a 48 hour period. The THUNDER Imager Live Cell provides a complete microscopy imaging system for minimally invasive and precise live cell imaging experimentation. Sensitive cell culture experiments can be efficiently conducted thanks to fast and High-Quantum-Efficiency camera options, accurate stages, tunable LED light sources, Computational Clearing to reduce out-of-focus blur in widefield images, and an easy to use LAS X software to automate imaging and analysis workflows.
Advantages of the THUNDER Imager Live Cell
- Accurate live cell imaging experimentation allows tracking of fast cell movements
- Low phototoxicity keep sensitive cell culture alive, even in long-term experiments
- Speed-up your live-cell imaging workflow up to automated quantification and analysis
![[Translate to french:] [Translate to italian:] [Translate to korean:] [Translate to portuquese:] [Translate to chinese:] One week cultured explants of abdominal aorta imaged for 48 hours on gelatin coated #1.5 coverglass chamber slides. The mouse was genetically encoded with a smooth muscle cell specific tdtomato. After a transcription event, the smooth muscle cells excise the tdtomato and begin to express eGFP.](/fileadmin/global/products/THUNDER/THUNDER_Imager_Live_Cell_Explant.jpg "[Translate to french:] [Translate to italian:] [Translate to korean:] [Translate to portuquese:] [Translate to chinese:] Explant Time-lapse")
Développement du pancréas du poisson zèbre
Le système THUNDER Imager 3D Assay a permis d'identifier clairement les cellules alpha (GFP-vert) et bêta (mCardinal-rouge) dans un pancréas de poisson zèbre en développement.
Imagé dans les canaux bleu (Hoechst), vert (GFP) et rouge (mCardinal), cette image z-stack de 150 images a été complétée avec tous les canaux en une minute.
Les conditions physiologiques de l'échantillon sont maintenues en minimisant le photoblanchiment, en fournissant des images de haute qualité et un débit élevé de données, ce qui améliore l'efficacité du workflow.
Images fournies avec l'aimable autorisation de Radhan Ramadass et Yu Hsuan du Max Planck Institute for Heart and Lung Research, Bad Nauheim (Allemagne)
Image at the speed of life – Cellular processes
Life is fast, especially for a cell. Most modern live cell imaging experiments demand high-speed systems.
THUNDER Imager Live Cell provides you with the power of highly sensitive sCMOS camera-based fluorescence systems working with full frames in one shot.
In combination with high sensitivity, THUNDER Imager Live Cell allows you to capture data at up to 90 frames per second, helping you to observe fast cellular events. It captures blur-free image data quickly even deep inside a thick 3D cell cluster. You can stay on top of fast processes even during multiple emission wavelength experiments thanks to fast-switching external filter wheels (< 27 ms).
Keep the right environment while observing your live cell cultures
THUNDER Imager Live Cell has all you need to maintain your cells at a close to natural state. The incubator ensures optimal physiological conditions for living cell cultures, such as system stability, humidity, temperature, and carbon dioxide level (pH value).
Thanks to the water immersion microdispenser, you can use a water-immersion objective for multiwell workflows even when you are doing long-term experiments. Water immersion objectives enable higher light collection leading to acquisition of cell images with higher contrast and resolution.
Master time-lapse multi-position experiments: tracking cell changes
THUNDER Imager Live Cell offer both speed and reliability for your 3D cell culture multiwell experiments. For example, when tracking the growth and development of spheroids and organoids, its speed and reliability allows you to obtain excellent results.
Accurate time-lapse multi-position experiments and tracking of cell changes are achieved with this THUNDER Imager due to:
- Reliable drift correction with the Adaptive Focus Control (AFC)
- Software autofocus that compensates for changes in specimen position
- Reproducible Z-positioning with a precision of up to 20 nm (closed-loop focus)
Get more data points in less time with the new Quantum Stage. It moves rapidly and accurately (e.g. 10 positions per second) to all positions with an excellent repeatability (< ±0.25 µm), as there is no jiggling.
Routine, reliable data acquisition
THUNDER Imager Live Cell enable reliable image data acquisition with accurate focus maintained on the live cells at all times.
Live cell imaging is often tricky, because of drift, morphology change, or growth of the cells. Drift is caused by vibrations, mechanical creep, or temperature fluctuations. Both drift and cell changes can reduce the reliability of the acquired image data, as focus becomes an issue. Thanks to the Adaptive Focus Control (AFC), closed-loop focus, and software autofocus, THUNDER Imager Live Cell reliably maintains focus for your multi well experiments.
Developing Zebrafish Pancreas
The THUNDER Imager 3D Assay enabled clear identification of Alpha (GFP-green) and Beta (mCardinal-red) cells within a developing zebrafish pancreas.
Imaged in the blue (Hoechst), green (GFP), and red (mCardinal) channels, this 150-image z-stack was completed with all channels within a minute.
Physiological conditions can be maintained within a sample by minimizing photobleaching, providing high-performance imaging, and high-throughput of data, leading to better workflow efficiency.
![[Translate to french:] Developing zebrafish pancreas - Widefield image](/fileadmin/_processed_/7/d/csm_Radhan-Zebrafish_Panc-DAPI_GFP-alpha_mCard-Beta_RFP-gamma_before_01_fdbf92c9c7.jpg "[Translate to french:] Developing zebrafish pancreas - Widefield image")
![[Translate to french:] After Computational Clearing - THUNDER Imager 3D Assay](/fileadmin/_processed_/9/f/csm_Radhan-Zebrafish_Panc-DAPI_GFP-alpha_mCard-Beta_RFP-gamma_01_ed79e6d210.jpg "[Translate to french:] After Computational Clearing - THUNDER Imager 3D Assay")
Image and manipulate your cell culture experiment
Combine THUNDER’s non-invasive imaging with photomanipulation, FRAP, FRET, or ablative techniques when using the Infinity Scanner. Manipulate the cells you study, or their extracellular environment, with the Infinity Scanner’s flexible vector laser-scanning system.
The two videos here show MDCK cells expressing mx1-GFP before, and after THUNDER Instant Computational Clearing. By using THUNDER, the mx1 protein expression became much more identifiable, and could be ablated with the Infinity Scanner.
Combine the THUNDER Imager Live Cell with the Infinity Scanner to image and manipulate your cell culture experiments.
Clarity and speed for sensitive sample imaging
Combine THUNDER’s out-of-focus blur removal with the advantages of TIRF. For dynamic processes at the cell surface, Total Internal Fluorescence Microscopy provides outstanding signal-to-background separation.
The two videos here show ins-1 cells expressing human pro-insulin with GFP-GRINCH. As KCL is added to the cell culture, the insulin-producing cells depolarize, and insulin residues can be seen fusing to the plasma membrane.
With the THUNDER Imager Live Cell combined with TIRF, image your sensitive samples with outstanding clarity, speed, and imaging parameter control.
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