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Ultramicrotomo per il sezionamento perfetto a temperatura ambiente e criogenico Leica EM UC7

  • Coltura cellulare

    Crescere le cellule in laboratorio rappresenta la base per le attività scientifiche nei campi della biologia cellulare, ricerca sul cancro, biologia dello sviluppo e in diversi altri campi di ricerca…
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  • Ricerca zebrafish

    Per ottenere il miglior risultato nella visualizzazione, separazione, manipolazione e imaging è necessario riuscire a osservare i dettagli e le strutture, così da poter prendere la decisione giusta…
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  • Microscopia a fluorescenza

    La fluorescenza è uno dei fenomeni fisici più comunemente utilizzati nella microscopia biologica e analitica, soprattutto a causa della sua elevata sensibilità e specificità. Scopri come i microscopi…
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  • Microscopi per geologia, paleontologia, scienze ambientali e della terra

    Research and discover solutions for routine to challenging environmental conservation and geoscience issues with versatile and reliable microscope systems from Leica Microsystems.
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  • Microscopi per la didattica delle Life Sciences

    Voi insegnanti di biologia potete ora mostrare agli studenti la struttura degli organismi viventi, utilizzando sezioni di tessuto e sangue come campioni per il microscopio. Per ottenere il massimo…
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  • Medicinal Use of Qianhu Plant Roots

    This study focused on tissue-specific metabolite profiling and comparison of various parts of bolting and unbolting Peucedanum praeruptorum Dunn (Qianhu in Chinese). The qualitative and quantitative…
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  • Ultrastructural Preservation and Improved Visualization of Membranes in Primary Bovine Chromaffin Cells

    Intracellular dynamic events, such as protein trafficking, recycling, and degradation as well as signal transduction, to name a few, can be studied using live-cell imaging techniques. However,…
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  • Introduction to Ultramicrotomy

    When studying samples, to visualize their fine structure with nanometer scale resolution, most often electron microscopy is used. There are 2 types: scanning electron microscopy (SEM) which images the…
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  • Bridging Structure and Dynamics at the Nanoscale through Optogenetics and Electrical Stimulation

    Nanoscale ultrastructural information is typically obtained by means of static imaging of a fixed and processed specimen. However, this is only a snapshot of one moment within a dynamic system in…
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  • Superior Ultrastructural Preservation and Structural Contrast in Drosophila Tissue

    Optimal structural preservation of tissue can only be obtained by high pressure freezing. However, preparing the samples in optimal conditions is challenging. This article explains in detail how to…
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  • Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions

    Human peripheral blood contains RNA in cells and in extracellular membrane vesicles, microvesicles and exosomes, as well as in cell-free ribonucleoproteins. Circulating mRNAs and noncoding RNAs, being…
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  • Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) of Yeast

    Application Note for Leica EM HPM100 - The sections are of yeast frozen with a Leica HPM100 high pressure freezer in the copper tube system, the cell paste was mixed with a pH 6.5 MES/dextran buffer…
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  • Immuno - Electron Microscopy of High Pressure Frozen and Freeze Substituted Mouse Heart

    Application Note for Leica EM AFS2 - Mouse heart tissue from wild-type (WT) and αT -catenin KO (KO) mice was excised, immersed in 20% (w/v) BSA and frozen immediately in a high-pressure freezer Leica…
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  • Cell Envelope Structure of a Gram-negative Thermotolerant y-Proteobacterium Acidithiobacillus sp., Strain HV2/2 and its Interaction with Pyrite

    Application Note for Leica EM AFS2 - Cells of the the moderately thermophilic Acidithiobacillus sp.strain HV2/2, were centrifuged at 20,0009xg and then cryo-immobilized by high-pressure freezing on…
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  • Symmetric Synapse - Clathrin Coated Endocytosis Pit in the Postsynaptic Dendrite

    Application Note for Leica EM ICE - WT hippocampal neurons were plated at a density of 80,000 cells/cm2 on 6 mm sapphire disks for 14 days. Sample were frozen using a high-pressure freezer (Leica EM…
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  • Electron Microscopy of High Pressure Frozen and Freeze Substituted Arabidopsis Thaliana Root Tips Cells

    Application Note for Leica EM AFS2 - Arabidopsis thaliana roots (mutant PIN1pro:PIN1-GFP;bex5-1) were excised, immersed in 20% (w/v) BSA and frozen immediately in a high-pressure freezer. Freeze…
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  • Electron Microscopy Sample Preparation: “The Future is Cold, Dynamic and Hybrid”

    In 2014, the renowned Electron Microscopy for Materials Science (EMAT) research lab at the University Antwerp, Belgium, and Leica Microsystems started a fruitful collaboration to establish a Leica…
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  • Transmission Electron Microscopy Sample Preparation Protocols for the Ultrastructural Study of Cysts of Free-living Protozoa

    Cysts of free-living protozoa have an impact on the ecology and epidemiology of bacteria because they may act as a transmission vector or shelter the bacteria against hash environmental conditions.…
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  • Correlative In-Resin Super-Resolution and Electron Microscopy Using Standard Fluorescent Proteins

    We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer…
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  • Influence of Tissue and Plant Species in the Trafficking of a Recombinant Protein in Plant Cells

    The development of recombinant DNA technology has allowed the use of plants for the production of biopharmaceuticals. In contrast to other production platforms, plants are unexpensive, easy to scale…
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  • Protein Trafficking in Cereal Seeds

    Seeds accumulate proteins and starch which will be broken down and mobilized upon germination. Among seeds, cereals constitute an example of a highly specialized storage tissue that constitutes up to…
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  • Perusing Alternatives for Automated Staining of TEM Thin Sections

    Contrast in transmission electron microscopy (TEM) is mainly produced by electron scattering at the specimen: Structures that strongly scatter electrons are referred to as electron dense and appear as…
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  • Atomic Force Microscopy Study of a Stretched Impact Copolymer

    Atomic force microscopy (AFM) is a powerful tool in the suite of nanoscale characterization techniques that provides a variety of information including topography, mechanical properties, and…
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  • Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

    This protocol describes how biological samples, like brain tissue, can be imaged in three dimensions using the focussed ion beam/scanning electron microscope (FIB/SEM). The samples are fixed with…
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  • Advanced Correlative Light/Electron Microscopy: Current Methods and New Developments Using Tokuyasu Cryosections

    Microscopy is an essential tool for analysis of cellular structures and function. With the advent of new fluorescent probes and super-resolution light microscopy techniques, the study of dynamic…
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