Revestimento a alto vácuo Leica EM ACE600
Automotivo e transporte microscópios
Nós, aqui na Leica, desejamos ser seu colaborador com o qual pode confiar, a fim de orientá-lo quanto às soluções ideais em captura e processamento de imagens, para que você possa estar à frente da…
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Cultura de células
Células crescendo sob condições laboratoriais são a base para cientistas nas áreas de biologia celular, pesquisa de combate ao câncer, biologia do desenvolvimento ou qualquer outra área em ciências da…
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Science Lab Topic:
EM Sample Preparation
Workflows and Instrumentation for Cryo-electron Microscopy
Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…
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Science Lab Topic:
EM Sample Preparation
Studying the Microstructure of Natural Polymers in Fine Detail
The potential of cryogenic broad ion beam milling used in combination with scanning electron microscopy (cryo-BIB-SEM) for imaging and analyzing the microstructure of cryogenically stabilized soft…
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Science Lab Topic:
EM Sample Preparation
Cryo-SEM analysis of UV light stimulated sun screen lotion
The EM ICE is the only available high-pressure freezer combining light stimulation with fast cryo fixation, a module mostly used for optogenetic approaches in neurobiology studies. However, this…
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Science Lab Topic:
EM Sample Preparation
Visualization of DNA Molecules
Precise low angle rotary shadowing with heavy metals (like platinum) can be used in transmission electron microscopy (TEM) to observe molecular details of objects previously absorbed on a thin, low…
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Science Lab Topic:
EM Sample Preparation
Carbon Coating for Polymeric Materials
Application Note fo Leica EM ACE600 - A solid understanding of polymer property-structure relationships is critical to improve and shorten development routes to new products. A direct way to determine…
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Science Lab Topic:
EM Sample Preparation
High-Resolution Carbon Coating: How much Carbon is too much?
Application Note for Leica EM ACE600 - Carbon support films are routinely used for high resolution TEM. Thickness is one of the main criteria to assess its usefulness for a particular experiment.…
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Science Lab Topic:
EM Sample Preparation
Each Atom Counts: Protect Your Samples Prior to FIB Processing
Application Note for Leica EM ACE600 - Focused ion beam (FIB) technology has become an indispensable tool for site-specific TEM sample preparation. It allows to extract electron transparent specimens…
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Science Lab Topic:
EM Sample Preparation
Ultra-thin Carbon Support Films for Improved STEM-EELS Analysis of Nanoparticles
Application Note for Leica EM ACE600 - Recent developments in aberration corrected transmission electron microscopes as well as further improvements in monochromaters and spectrometers have pushed the…
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Science Lab Topic:
EM Sample Preparation
Ways to Reveal More from your Samples: Ultra-Thin Carbon Films
Application Note for Leica EM ACE600 - Much of the battle involved in obtaining good transmission electron microscopy data is in the specimen preparation itself. Even though some nanomaterials are…
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Science Lab Topic:
EM Sample Preparation
Cryo-SEM Imaging of Latex Paint
Application Note for Leica EM VCT100, Leica EM ACE600 - A thin layer of latex paint was spread on a clean, scored, silicon chip. The sample was immediately plunge frozen in liquid ethane and…
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Science Lab Topic:
EM Sample Preparation
Improvement of Metallic Thin Films for HR-SEM by Using DC Magnetron Sputter Coater
Preparation techniques, like several kinds of coating methods play an important role for high resolution scanning electron microscopy (HR-SEM). Nonconductive sample like biological and synthetic…
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Science Lab Topic:
EM Sample Preparation
High Pressure Freezing with Light Stimulation
Sun screen lotion was carefully filled in the 100 μm incision of a 3 mm copper/gold plated flat carrier and covered with 3 mm sapphire disk. The sun screen lotion sample was then high pressure frozen…
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Science Lab Topic:
EM Sample Preparation
Electron Microscopy Sample Preparation: “The Future is Cold, Dynamic and Hybrid”
In 2014, the renowned Electron Microscopy for Materials Science (EMAT) research lab at the University Antwerp, Belgium, and Leica Microsystems started a fruitful collaboration to establish a Leica…
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Science Lab Topic:
EM Sample Preparation
How to Clean a Coater
Coating of samples is required in the field of electron
microscopy to enable or improve the imaging of samples. Compared to the traditional coater design, all parts of a Leica EM ACE Coater can be…
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Science Lab Topic:
EM Sample Preparation
Glycerol Spraying/Platinum Low Angle Rotary Shadowing of DNA with the Leica EM ACE600 e-beam
Glycerol spraying/low angle rotary shadowing (Aebi and Baschong, 2006) is a preparation technique used in biology to visualize structures yielding insufficent contrast with other techniques, due to…
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Science Lab Topic:
EM Sample Preparation
Brief Introduction to Coating Technology for Electron Microscopy
Coating of samples is required in the field of electron microscopy to enable or improve the imaging of samples. Creating a conductive layer of metal on the sample inhibits charging, reduces thermal…
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Science Lab Topic:
EM Sample Preparation
Carbon Thickness Evaluation in Electron Microscopy
The coating layers applied and used for electron microscopy imaging are commonly controlled and measured by quartz crystals. These crystals oscillate with a certain frequency (around 6 megahertz when…
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