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Microdisección láser Leica LMD6 & LMD7

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

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DNA Research

Gene therapy restores mitochondrial function and protects retinal ganglion cells in optic neuropathy induced by a mito-targeted mutant ND1 gene
Liu Y, Eastwood JD, Alba DE, Velmurugan S, Sun N, Porciatti V, Lee RK, Hauswirth WW, Guy J, Yu H:
Gene Ther. 2022 Apr 6. doi: 10.1038/s41434-022-00333-6.

RNF43 as a predictor of malignant transformation of pancreatic mucinous cystic neoplasm
Sakihama K, Koga Y, Yamamoto T, Shimada Y, Yamada Y, Kawata J, Shindo K, Nakamura M, Oda Y:
Virchows Arch. 2022 Jan 23. doi: 10.1007/s00428-022-03277-9.

Whole-Exome Sequencing of HPV Positive Tonsillar and Base of Tongue Squamous Cell Carcinomas Reveals a Global Mutational Pattern along with Relapse-Specific Somatic Variants
Ährlund-Richter A, Holzhauser S, Dalianis T, Näsman A, and Mints M:
Cancers 2022, 14, 77. https://doi.org/10.3390/cancers14010077

RNA Research

Laser Microdissection for Species-agnostic Single-tissue Applications
Woronik A, Kiontke K, Jallad RS, Herrera RA, Fitch DHA:
J Vis Exp. 2022 Mar 31;(181). doi: 10.3791/63666.

Effects of progesterone on T-type-Ca2+-channel expression in Purkinje cells
Eickhoff A, Tjaden J, Stahlke S, Vorgerd M, Theis V, Matschke V, Theiss C:
Neural Regeneration Research: Nov 2022, Vol. 17, Issue 11, doi: 10.4103/1673-5374.339008

RNA Sequencing of Epithelial Cell/Fibroblastic Foci Sandwich in Idiopathic Pulmonary Fibrosis: New Insights on the Signaling Pathway
Calabrese F, Lunardi F, Tauro V, Pezzuto F, Fortarezza F, Vedovelli L, Faccioli E, Balestro E, Schiavon M, Esposito G, Vuljan SE, Giraudo C, Gregori D, Rea F, Spagnolo P:
Int J Mol Sci. 2022 Mar 19;23(6):3323. doi: 10.3390/ijms23063323. PMID: 35328744

Proteomics, Lipidomics, Metabolomics

Morphogenesis, ultrastructure, and chemical profiling of trichomes in Artemisia argyi H. Lév. & Vaniot (Asteraceae)
Cui, Z., Li, M., Han, X., Liu, H., Li, C., Peng, H., Liu, D., Huang, X., and Zhang, Z.:
Planta 255, 102 (2022).

Disease-related protein co-expression networks are associated with the prognosis of resectable node-positive pancreatic ductal adenocarcinoma
Nishimura T, Takadate T, Maeda S, Suzuki T, Minowa T, Fukuda T, Bando Y, Unno M:
Research Square, April 14th 2022, DOI: doi.org/10.21203/rs.3.rs-1539065/v1

Proteomic differences in hippocampus and cortex of sudden unexplained death in childhood
Leitner DF, William C, Faustin A, Askenazi M, Kanshin E, Snuderl M, McGuone D, Wisniewski T, Ueberheide B, Gould L, Devinsky O:
Acta Neuropathol. 2022 Mar 25. doi: 10.1007/s00401-022-02414-7.

Fluorescence Applications

Lower Levels of GABAergic Function Markers in Corticotropin-Releasing Hormone-Expressing Neurons in the sgACC of Human Subjects With Depression
Oh H, Newton D, Lewis D, Sibille E:
Front Psychiatry. 2022 Feb 25;13:827972. doi: 10.3389/fpsyt.2022.827972. eCollection 2022.

The Amyloid Plaque Proteome in Early Onset Alzheimer’s Disease and Down Syndrome
Drummond E, Kavanagh T, Pires G, Ariza MM, Kanshin E, Nayak S, Faustin A, Berdah V, Ueberheide B, Wisniewski T:
Resrearch Square, 8 Feb 2022, doi: 10.21203/rs.3.rs-1316035/v1

Comparative analysis in different organs and tissue-specific metabolite profiling of Atractylodes lancea from four regions by GC-MS and laser microdissection
Xu R, Lu J, Wu J, Yu D, Chu S, Guan F, Liu W, Hu J, Peng H, Zha L:
J Sep Sci. 2021 Dec 28. doi: 10.1002/jssc.202100924.

Cancer Research

Disease-related protein co-expression networks are associated with the prognosis of resectable node-positive pancreatic ductal adenocarcinoma
Nishimura T, Takadate T, Maeda S, Suzuki T, Minowa T, Fukuda T, Bando Y, Unno M:
Research Square, April 14th 2022, DOI: 10.21203/rs.3.rs-1539065/v1

Creating a ‘Timeline’ of ductal carcinoma in situ to identify processes and biomarkers for progression towards invasive ductal carcinoma
Rebbeck CA, Xian J, Bornelöv S, Geradts J, Hobeika A, Geiger H, Alvarez JF, Rozkhova E, Nicholls A, Robine N, Lyerly HK, Hannon GJ:
bioRxiv, 4 Mar 2022, doi: 10.1101/2022.03.01.482529

Spatiotemporal dynamics of clonal selection and diversification in normal endometrial epithelium.
Yamaguchi M, Nakaoka H, Suda K, Yoshihara K, Ishiguro T, Yachida N, Saito K, Ueda H, Sugino K, Mori Y, Yamawaki K, Tamura R, Revathidevi S, Motoyama T, Tainaka K, Verhaak RGW, Inoue I, Enomoto T:
Nat Commun. 2022 Feb 17;13(1):943. doi: 10.1038/s41467-022-28568-2.

Neurosciences

Effects of progesterone on T-type-Ca2+-channel expression in Purkinje cells
Eickhoff A, Tjaden J, Stahlke S, Vorgerd M, Theis V, Matschke V, Theiss C:
Neural Regeneration Research: Nov 2022, Vol. 17, Issue 11, doi: 10.4103/1673-5374.339008

Proteomic differences in hippocampus and cortex of sudden unexplained death in childhood
Leitner DF, William C, Faustin A, Askenazi M, Kanshin E, Snuderl M, McGuone D, Wisniewski T, Ueberheide B, Gould L, Devinsky O:
Acta Neuropathol. 2022 Mar 25. doi: 10.1007/s00401-022-02414-7.

Impact of Non-pharmacological Chronic Hypertension on a Transgenic Rat Model of Cerebral Amyloid Angiopathy
Stanisavljevic A, Schrader JM, Zhu X, Mattar JM, Hanks A, Xu F, Majchrzak M, Robinson JK and Van Nostrand WE:
Front. Neurosci., 15 March 2022 | doi: 10.3389/fnins.2022.811371 

Clinical/Pathology/Immunology Research Applications

Gene therapy restores mitochondrial function and protects retinal ganglion cells in optic neuropathy induced by a mito-targeted mutant ND1 gene
Liu Y, Eastwood JD, Alba DE, Velmurugan S, Sun N, Porciatti V, Lee RK, Hauswirth WW, Guy J, Yu H:
Gene Ther. 2022 Apr 6. doi: 10.1038/s41434-022-00333-6.

RNA Sequencing of Epithelial Cell/Fibroblastic Foci Sandwich in Idiopathic Pulmonary Fibrosis: New Insights on the Signaling Pathway
Calabrese F, Lunardi F, Tauro V, Pezzuto F, Fortarezza F, Vedovelli L, Faccioli E, Balestro E, Schiavon M, Esposito G, Vuljan SE, Giraudo C, Gregori D, Rea F, Spagnolo P:
Int J Mol Sci. 2022 Mar 19;23(6):3323. doi: 10.3390/ijms23063323. PMID: 35328744

AL-Kappa Primary Amyloidosis with Apolipoprotein A-IV Deposition
Ishimitsu A, Tojo A, Hirao J, Yokoyama S, Ohira T, Murayama Y, Ishimitsu T, Kang D, Honda K, Ehara T, Ishida K, Ueda Y :
Intern Med. 2022;61(6):871-876. doi: 10.2169/internalmedicine.7955-21. Epub 2022 Mar 15.

Developmental Research, Epigenetics  & Aging

Laser Microdissection for Species-agnostic Single-tissue Applications
Woronik A, Kiontke K, Jallad RS, Herrera RA, Fitch DHA:
J Vis Exp. 2022 Mar 31;(181). doi: 10.3791/63666.

Intracrine activity involving NAD-dependent circadian steroidogenic activity governs age-associated meibomian gland dysfunction
Sasaki L, Hamada Y, Yarimizu D, Suzuki T, Nakamura H, Shimada A, Nguyen Pham KT, Shao X, Yamamura K, Inatomi T, Morinaga H, Nishimura EK, Kudo F, Manabe I, Haraguchi S, Sugiura Y, Suematsu M, Kinoshita S, Machida M, Nakajima T, Kiyonari H, Okamura H, Yamaguchi Y, Miyake T, and Doi M:
Nat Aging (10 Feb 2022). doi: 10.1038/s43587-021-00167-8

Age-related downregulation of CCN2 is regulated by cell size in a YAP/TAZ-dependent manner in human dermal fibroblasts: impact on dermal aging
Qin Z, He T, Guo C, Quan T:
JID Innovations, 2022, 100111, ISSN 2667-0267, doi: 10.1016/j.xjidi.2022.100111

Plant Research

Morphogenesis, ultrastructure, and chemical profiling of trichomes in Artemisia argyi H. Lév. & Vaniot (Asteraceae)
Cui, Z., Li, M., Han, X., Liu, H., Li, C., Peng, H., Liu, D., Huang, X., and Zhang, Z.:
Planta 255, 102 (2022).

Xylan-based nanocompartments orchestrate plant vessel wall patterning
Wang H, Yang H, Wen Z, Gao C, Gao Y, Tian Y, Xu Z, Liu X, Persson S, Zhang B, Zhou Y:
Nat Plants. 2022 Mar;8(3):295-306. doi: 10.1038/s41477-022-01113-1. Epub 2022 Mar 22.

Expression of the VvMYB60 Transcription Factor Is Restricted to Guard Cells and Correlates with the Stomatal Conductance of the Grape Leaf
Simeoni F, Simoni L, Zottini M, Conti L, Tonelli C, Castorina G, Espen L, and Galbiati M:
Agronomy 2022, 13 March, 12(3), 694; doi: 10.3390/agronomy12030694

Forensics

Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
Sun M, Zheng J, Wnag B and Yao J:
BioMed Research International, Volume 2021 | Article ID 7269237

Application and utility of alternative methods in isolation of pure cells from forensic biological mixtures in modern-day: a review
Prajapati S, Rajmane P, Jayakrishna P, Nair MS, Kshirsagar P and Meshram M:
JFSR – Journal of Forensic Science and Research, Aug 2021, ISSN 2575-0186

Recent trends and developments in forensic DNA extraction
Chong KWY, Thong Z, Kiu-Choong Syn C :
WIREs Forensic Science, 30 July 2020, DOI: 10.1002/wfs2.1395

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Further information on Leica LMD impact at forensics: 
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Identification of NFASC and CHL1 as Two Novel Hub Genes in Endometriosis Using Integrated Bioinformatic Analysis and Experimental Verification
Chen P, Yao M, Fang T, Ye C, Du Y, Jin Y, Wu R :
Pharmacogenomics and Personalized Medicine, 22 Apr 2022, Volume 15 : Pages 377—392

Berberine maintains intestinal homeostasis through modulating TLR4/ NF-κB / MTORC pathway and autophagy in cats
Guo MY, Cao JW, Chen My, Li X:
Research Square, April 13th 2022, DOI: 0.21203/rs.3.rs-1537283/v1

Comparison of Computational Tools for Differential Gene Expression Analysis of RNA Sequencing and Single-Cell RNA Sequencing Data
Menon S, Chi Hang Lui V, Kwong Hang Tam P:
ejbps, 2022, Volume 9, Issue 4, 86-94

Live Cell Culture

Insight into the Molecular Characteristics of Langhans Giant Cell by Combination of Laser Capture Microdissection and RNA Sequencing
Chen Y, Jiang H, Xiong J, Shang J, Chen Z, Wu A, Wang H:
Journal of Inflammation Research, 2 Feb 2022,  Vol. 15, doi: 10.2147/JIR.S337241

Cell position within human pluripotent stem cell colonies determines apicalspecialization via an actin cytoskeleton-based mechanism
Kim Y, Jang H, Seo K, Kim JH, Cho HM, Kim HJ, Yang E, Kim H, Gim J-A, Park Y, Ryu JR, Sun W:
Stem Cell Reports. 2021 Nov 27;S2213-6711(21)00586-5. doi: 10.1016/j.stemcr.2021.11.005.

Enriched transcriptome analysis of laser capture microdissected populationsof single cells to investigate intracellular heterogeneity in immunostainedFFPE sections
Hammoudeh SM, Hammoudeh AM, Venkatachalam T, Rawat S, Jayakumar MN, Rahmani M, and Hamoudi R:
Computational and Structural Biotechnology Journal, 9 Sep 21, S2001-0370(21)00395-0