Leica LMD6 & LMD7 Microdisección láser

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

Deciphering functional tumor–immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann M
bioRxiv preprint, May 23, 2024

The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma
Hu H, Liu G, Li Y
Plant Methods. 2024 May 17;20(1):72. doi: 10.1186/s13007-024-01184-5.

Deep Visual Proteomics advances human colon organoid models by revealing a switch to an in vivo-like phenotype upon xenotransplantation
Post F, Hausmann A, Kabatnik S, Steigerwald S, Brand A, Clement DL, Skov J, Boyce TL, Sato T, Steenholdt C, Mund A, Nielsen OH, Jensen KB, Mann M
bioRxiv preprint, doi: doi.org/10.1101/2024.05.13.593888; May 14, 2024

DNA Research

Oral pimonidazole unveils clinicopathologic and epigenetic features of hypoxic tumour aggressiveness in localized prostate cancer
Ci X, Chen S, Zhu R, Zarif M, Jain R, Guo W, Ramotar M, Gong L, Xu W, Singh O, Mansouri S, Zadeh G, Wei GH, Xu W, Bristow R, Berlin A, Koritzinsky M, van der Kwast T, He HH
BMC Cancer. 2024 Jun 18;24(1):744. doi: 10.1186/s12885-024-12505-1.

Genetic and epigenetic alterations in precursor lesions of endometrial endometrioid carcinoma
Gotoh O, Sugiyama Y, Tonooka A, Kosugi M, Kitaura S, Minegishi R, Sano M, Amino S, Furuya R, Tanaka N, Kaneyasu T, Kumegawa K, Abe A, Nomura H, Takazawa Y, Kanao H, Maruyama R, Noda T, Mori S
J Pathol. 2024 May 11. doi: 10.1002/path.6278.

Targeting pathological cells with senolytic drugs reduces seizures in neurodevelopmental mTOR-related epilepsy
Ribierre T, Bacq A, Donneger F, Doladilhe M, Maletic M, Roussel D, Le Roux I, Chassoux F, Devaux B, Adle-Biassette H, Ferrand-Sorbets S, Dorfmüller G, Chipaux M, Baldassari S, Poncer JC, Baulac S
Nat Neurosci. 2024 May 6. doi: 10.1038/s41593-024-01634-2.

RNA Research

Differential root and cell regulation of maize aquaporins by the arbuscular mycorrhizal symbiosis highlights its role in plant water relations
Romero-Munar A, Muñoz-Carrasco M, Balestrini R, De Rose S, Giovannini L, Aroca R, Ruiz-Lozano JM
Plant Cell Environ. 2024 Jul 4. doi: 10.1111/pce.15029.

An optimized workflow for transcriptomic analysis from archival paraformaldehyde-fixed retinal tissues collected by laser capture microdissection
Takahashi K, Beltran WA, Sudharsan R
Exp Eye Res. 2024 Jul 3:109989. doi: 10.1016/j.exer.2024.109989.

Dorsomorphin inhibits AMPK, upregulates Wnt and Foxo genes and promotes the activation of dormant follicles
Madsen JF, Ernst EH, Amoushahi M, Dueholm M, Ernst E, Lykke-Hartmann K
Commun Biol. 2024 Jun 20;7(1):747. doi: 10.1038/s42003-024-06418-9.

Proteomics, Lipidomics, Metabolomics

Enhanced in situ spatial proteomics by effective combination of MALDI imaging and LC-MS/MS
Schäfer F, Tomar A, Sato S, Teperino R, Imhof A, Lahiri S
Mol Cell Proteomics. 2024 Jul 10:100811. doi: 10.1016/j.mcpro.2024.100811.

Proteomic Profiling of Laser Capture Microdissection Kidneys from Diabetic Nephropathy Patients
Yang Z, Chen L, Huang Y, Dong J, Yan Q, Li Y, Qiu J, Li H, Zhao D, Liu F, Tang D, Dai Y
Journal of Chromatography B, 5 July 2024, 124231

Effect of dynamic exclusion and the use of FAIMS, DIA and MALDI-mass spectrometry imaging with ion mobility on amyloid protein identification
Aguilan JT, Lim J, Racine-Brzostek S, Fischer J, Silvescu C, Cornett S, Nieves E, Mendu DR, Aliste CM, Semple S, Angeletti R, Weiss LM, Cole A, Prystowsky M, Pullman J, Sidoli S
Clin Proteomics. 2024 Jul 3;21(1):47. doi: 10.1186/s12014-024-09500-w.

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8
Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Fluorescence Applications

Deciphering functional tumor–immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann M
bioRxiv preprint, May 23, 2024

The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma
Hu H, Liu G, Li Y
Plant Methods. 2024 May 17;20(1):72. doi: 10.1186/s13007-024-01184-5.

Deep Visual Proteomics advances human colon organoid models by revealing a switch to an in vivo-like phenotype upon xenotransplantation
Post F, Hausmann A, Kabatnik S, Steigerwald S, Brand A, Clement DL, Skov J, Boyce TL, Sato T, Steenholdt C, Mund A, Nielsen OH, Jensen KB, Mann M
bioRxiv preprint, doi: doi.org/10.1101/2024.05.13.593888; May 14, 2024

Cancer Research

Oral pimonidazole unveils clinicopathologic and epigenetic features of hypoxic tumour aggressiveness in localized prostate cancer
Ci X, Chen S, Zhu R, Zarif M, Jain R, Guo W, Ramotar M, Gong L, Xu W, Singh O, Mansouri S, Zadeh G, Wei GH, Xu W, Bristow R, Berlin A, Koritzinsky M, van der Kwast T, He HH
BMC Cancer. 2024 Jun 18;24(1):744. doi: 10.1186/s12885-024-12505-1.

Integrating artificial intelligence-based computational pathology approaches with laser microdissection for tumor enrichment and cancer research
Chain DC
Thesis, 2024

3D genomic mapping reveals multifocality of human pancreatic precancers
Braxton AM, Kiemen AL, Grahn MP, Forjaz A, Parksong J, Mahesh Babu J, Lai J, Zheng L, Niknafs N, Jiang L, Cheng H, Song Q, Reichel R, Graham S, Damanakis AI, Fischer CG, Mou S, Metz C, Granger J, Liu XD, Bachmann N, Zhu Y, Liu Y, Almagro-Pérez C, Jiang AC, Yoo J, Kim B, Du S, Foster E, Hsu JY, Rivera PA, Chu LC, Liu F, Fishman EK, Yuille A, Roberts NJ, Thompson ED, Scharpf RB, Cornish TC, Jiao Y, Karchin R, Hruban RH, Wu PH, Wirtz D, Wood LD
Nature. 2024 May 1. doi: 10.1038/s41586-024-07359-3.

Neurosciences

Enhanced in situ spatial proteomics by effective combination of MALDI imaging and LC-MS/MS
Schäfer F, Tomar A, Sato S, Teperino R, Imhof A, Lahiri S
Mol Cell Proteomics. 2024 Jul 10:100811. doi: 10.1016/j.mcpro.2024.100811.

Improved leptin sensitivity and increased soluble leptin receptor concentrations may underlie the additive effects of combining PYY (3-36) and exendin-4 on body weight lowering in diet-induced obese mice
Wulff BS, Kuhre RE, Selvaraj M, Rehfeld JF, Niss K, Fels JJ, Secher A, Raun K, Gerstenberg MK
HELIYON, 27. May 2024.

Proteomic dissection of vanishing white matter pathogenesis
Man JHK, Zarekiani P, Mosen P, de Kok M, Debets DO, Breur M, Altelaar M, van der Knaap MS, Bugiani M
Cell Mol Life Sci. 2024 May 24;81(1):234. doi: 10.1007/s00018-024-05258-4.

Clinical/Pathology/Immunology Research Applications

Layer-specific protSeomic profiling of human normal heart
Kakimoto Y, Ueda A, Kimura Y, Akiyama T, Tanaka M, Ikeda H, Isozaki S, Maeda K, Osawa M
Pathology - Research and Practice, 2024, 155453, ISSN 0344-0338

Proteomic Profiling of Laser Capture Microdissection Kidneys from Diabetic Nephropathy Patients
Yang Z, Chen L, Huang Y, Dong J, Yan Q, Li Y, Qiu J, Li H, Zhao D, Liu F, Tang D, Dai Y
Journal of Chromatography B, 5 July 2024, 124231

Effect of dynamic exclusion and the use of FAIMS, DIA and MALDI-mass spectrometry imaging with ion mobility on amyloid protein identification
Aguilan JT, Lim J, Racine-Brzostek S, Fischer J, Silvescu C, Cornett S, Nieves E, Mendu DR, Aliste CM, Semple S, Angeletti R, Weiss LM, Cole A, Prystowsky M, Pullman J, Sidoli S
Clin Proteomics. 2024 Jul 3;21(1):47. doi: 10.1186/s12014-024-09500-w.

Developmental Research, Epigenetics  & Aging

Dorsomorphin inhibits AMPK, upregulates Wnt and Foxo genes and promotes the activation of dormant follicles
Madsen JF, Ernst EH, Amoushahi M, Dueholm M, Ernst E, Lykke-Hartmann K
Commun Biol. 2024 Jun 20;7(1):747. doi: 10.1038/s42003-024-06418-9.

Cytomegalovirus infection lengthens the cell cycle of granule cell precursors during postnatal cerebellar development
Sung CYW, Li M, Jonjic S, Sanchez V, Britt WJ
JCI Insight. 2024 Jun 10;9(11):e175525. doi: 10.1172/jci.insight.175525.

Plasma and Kidney Proteome Profiling Combined with Laser Capture Microdissection Reveal Large Increases in Immunoglobulins with Age
Chan LJG; Olsson N; Preciado López M; Hake K; Tomono H; Veras MA; McAllister FE
Proteomes 2024; 12, 16. 3. June 2024. 

Plant Research

Differential root and cell regulation of maize aquaporins by the arbuscular mycorrhizal symbiosis highlights its role in plant water relations
Romero-Munar A, Muñoz-Carrasco M, Balestrini R, De Rose S, Giovannini L, Aroca R, Ruiz-Lozano JM
Plant Cell Environ. 2024 Jul 4. doi: 10.1111/pce.15029.

The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma
Hu H, Liu G, Li Y
Plant Methods. 2024 May 17;20(1):72. doi: 10.1186/s13007-024-01184-5.

LMD and LC-MS-based chemical constituents and pharmacological effects assessment for two different processing methods of the root of Paeonia lactiflora Pall
Xu C, Wang X, Han J, Gu Z, Guo Q
Journal of Pharmaceutical and Biomedical Analysis, 27 April 2024, 116184

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Further information on Leica LMD impact at forensics: 
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Oversampling for Enhanced Spatial Resolution of Zebrafish by Top-Hat IR-MALDESI-MSI
Sohn AL, Bowman AP, Barnes MM, Kullman SW, Muddiman DC
J Am Soc Mass Spectrom. 2024 Jul 10. doi: 10.1021/jasms.4c00219.

An optimized workflow for transcriptomic analysis from archival paraformaldehyde-fixed retinal tissues collected by laser capture microdissection
Takahashi K, Beltran WA, Sudharsan R
Exp Eye Res. 2024 Jul 3:109989. doi: 10.1016/j.exer.2024.109989.

Persistent activity of aerobic methane-oxidizing bacteria in anoxic lake waters due to metabolic versatility.
Schorn S, Graf JS, Littmann S, Hach PF, Lavik G, Speth DR, Schubert CJ, Kuypers MMM, Milucka J
Nat Commun. 2024 Jun 21;15(1):5293. doi: 10.1038/s41467-024-49602-5.

Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23

Live Cell Culture

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416

An efficient semi–in vivo zygotic embryogenesis system in Arabidopsis
Li J, Li SS, Liu Y, Tang WY, Liu XF, Meng CF, Zhang LY, Sun MX
In Vitro Cell.Dev.Biol.-Plant, 27. Dec 2022