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Leica SR GSD 3D Para microscopía de localización en 3D

La evolución de la resolución

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PtK2 cells with Alexa647 stained microtubules in red and Alexa488 stained clathrin in green

Courtesy: Prof. Dr. Stefan Hell, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany

Ptk2-cells. NPC-staining: anti-NUP153/Alexa FLUOR 532 Microtubule-staining: anti-β-tubulin/Alexa FLUOR 488

Courtesy: Wernher Fouquet, Leica Microsystems in collaboration with Anna Szymborsak and Jan…

Golgi body, B16 (Mouse melanoma cell line), Golgi targeting signal of β-1,4-galactosyltransferase, fused to EYFP

Courtesy: Dr. Yasushi Okada, Department of Cell Biology and Anatomy, Graduate School of…

Vimentin Filaments

3D reconstruction of a corner of a human endothelial cell (Huvec), stained for Vimentin with Alexa 647 Ab. Medium: gloxy-like buffer. Courtesy of K. Jalink and L. Nahidi Azar, Amsterdam, The…

Neuron synapse

3D reconstruction of  a synapse. Double staining of post- and presynaptic proteins Homer /Alexa647 (red) and Bassoon/ Alexa532 (green). Embedding media: MEA. Courtesy of Dr. W. Zuschratter, Dr. O.…

Mitochondrial ATP-Synthase

Comparison of a 3D reconstruction of GSD and wide-field images. Mitochondrial ATP-Synthase in MDCK cells was stained with anti-ATP-2B/Alexa647 and four image stacks each 800 nm in thickness were…

Vesicle Protein

MDCK cells stably transfected with a vesicle membrane marker protein fused to YFP. Recombinant Protein of interest coupled with Alexa 647 was endocytosed from the apical membrane of the cell. After…

3D reconstruction of neurons

3D reconstruction of neurons, double stained for postsynaptic protein Homer with Alexa647® (red) and presynaptic protein Bassoon with Alexa532® (green). Embedding medium: MEA. Courtesy of Dr. W.…

3D reconstruction of the synaptonemal complex protein 3 (SYCP3)

3D reconstruction of the synaptonemal complex protein 3 (SYCP3) stained with Alexa 647. Medium: 50 mM MEA in PBS. Five image stacks each 800 nm in thickness were merged. Courtesy of M. Lessard, Maine,…

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