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Studying Actin Cytoskeleton and Microfilament Assembly

This article demonstrates how actin microfilament assemblies can be clearly visualized with superior resolution at the plasma membrane of a cell using a DMi8 S Infinity TIRF High Power imaging…
Studying Actin Cytoskeleton and Microfilament Assembly

Plant Cell Development and Morphogenesis

The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence (TIRF) microscopy to better understand tubulin…
Plant Cell Development and Morphogenesis

Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex

Plants, Bacteria, and Fungi possess a rigid cell wall that protects the cell and gives it shape. Animal cells, such as mammalian cells, have no outer wall, which exposes their plasma membrane to the…
Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex

Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy

Total Internal Reflection Fluorescence (TIRF) Microscopy is a technique that only illuminates dye molecules near a surface. In this video, the pioneer of TIRF Microscopy describes what this technique…
Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy

Universal PAINT – Dynamic Super-Resolution Microscopy

Super-resolution microscopy techniques have revolutionized biology for the last ten years. With their help cellular components can now be visualized at the size of a protein. Nevertheless, imaging…
Universal PAINT – Dynamic Super-Resolution Microscopy

Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips

The widefield super-resolution technique GSDIM (Ground State Depletion followed by individual molecule return) is a localization microscopy technique that is capable of resolving details as small as…
Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips

Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results

The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…
Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results

Total Internal Reflection Fluorescence (TIRF) Microscopy

Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…
Total Internal Reflection Fluorescence (TIRF) Microscopy

Super-Resolution GSDIM Microscopy

The nanoscopic technique GSDIM (ground state depletion microscopy followed by individual molecule return) provides a detailed image of the spatial arrangement of proteins and other biomolecules within…
Super-Resolution GSDIM Microscopy

TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells

Application of TIRF microscopy (Total Internal Reflection Fluorescence) allows the visualization of structures at the apical surface of polarized epithelial cells that have been hidden in conventional…
TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells

Exploring Cell Logistics

Using TIRF microscopy, scientists have been able to take a closer look at intracellular transport processes with the example of the galactose-binding protein Galectin-3, which has been identified as a…
Exploring Cell Logistics

Domaines d'application

Imagerie des cellules vivantes

Passant de la perspective basée sur des composants de microscopes individuels à la solution d'imagerie des cellules vivantes parfaitement fonctionnelle, Leica Microsystems intègre le microscope, le…

Photomanipulation

Le terme de photomanipulation se réfère à une série de techniques qui utilisent les propriétés des molécules fluorescentes pour déclencher des événements et observer comment les ensembles dynamiques…

Microscopes à super-résolution et nanoscopes

Leica Microsystems est le pionnier de la microscopie de super-résolution, travaillant en collaboration étroite avec les meilleurs scientifiques dans ce domaine. Leica Microsystems est le seul…

Biologie cellulaire

Découvrez comment vous pouvez tirer le meilleur parti de vos recherches en biologie cellulaire et élargir vos découvertes grâce aux solutions d’imagerie de Leica Microsystems.

Virologie

Vos recherches portent sur les infections et les maladies virales ? Découvrez comment renforcer vos connaissances en virologie grâce aux solutions d’imagerie et de préparation d’échantillons de Leica…

Techniques avancées de microscopie

Les techniques de microscopie avancées englobent les techniques d'imagerie à haute résolution et à super-résolution. Ces techniques sont principalement utilisées pour visualiser des événements…
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