Especialidades médicas
Especialidades médicas
Explore uma coleção abrangente de recursos científicos e clínicos adaptados para profissionais de saúde, incluindo percepções de colegas, estudos de casos clínicos e simpósios. Projetado para neurocirurgiões, oftalmologistas e especialistas em cirurgia plástica e reconstrutiva, otorrinolaringologia e odontologia. Esta coleção destaca os mais recentes avanços em microscopia cirúrgica. Descubra como as tecnologias cirúrgicas de ponta, como fluorescência AR, visualização 3D e imagens intraoperatórias de OCT, possibilitam a tomada de decisões confiantes e a precisão em cirurgias complexas.
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Step by Step Guide for FRAP Experiments
Fluorescence Recovery After Photobleaching (FRAP) has been considered the most widely applied method for observing translational diffusion processes of macromolecules. The resulting information can be…
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Widefield Calcium Imaging with Calcium Indicator Fura2
In eukaryotic cells Ca2+ is one of the most widespread second messengers used in signal transduction pathways. Intracellular levels of Ca2+ are usually kept low, as Ca2+ often forms insoluble…
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Fluorescence Correlation Spectroscopy (FCS)
Fluorescence correlation spectroscopy (FCS) measures fluctuations of fluorescence intensity in a sub-femtolitre volume to detect such parameters as the diffusion time, number of molecules or dark…
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Fluorescence Recovery after Photobleaching (FRAP) and its Offspring
FRAP (Fluorescence recovery after photobleaching) can be used to study cellular protein dynamics: For visualization the protein of interest is fused to a fluorescent protein or a fluorescent dye. A…
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Förster Resonance Energy Transfer (FRET)
The Förster Resonance Energy Transfer (FRET) phenomenon offers techniques that allow studies of interactions in dimensions below the optical resolution limit. FRET describes the transfer of the energy…
