Microdissection laser Leica LMD6 & LMD7

La dissection parfaite

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

DNA Research

Spatial genomics maps the structure, nature and evolution of cancer clones
Lomakin A, Svedlund J, Strell C, Gataric M, Shmatko A, Rukhovich G, Park JS, Ju YS, Dentro S, Kleshchevnikov V, Vaskivskyi V, Li T, Bayraktar OA, Pinder S, Richardson AL, Santagata S, Campbell PJ, Russnes H, Gerstung M, Nilsson M, Yates LR
Nature. 2022 Nov 9. doi: 10.1038/s41586-022-05425-2.

Characterization of the evolution trajectory and immune profiling of new histologic patterns in lung adenocarcinoma
Song M, Xie H, Liu W, Zhao M, Deng Z, Zhang L, Liu H, Huang Y, Li H, Ren Y, Chen C
J Gene Med. 2022 Oct 4:e3455. doi: 10.1002/jgm.3455.

Ordered and deterministic cancer genome evolution after p53 loss
Baslan T, Morris JP 4th, Zhao Z, Reyes J, Ho YJ, Tsanov KM, Bermeo J, Tian S, Zhang S, Askan G, Yavas A, Lecomte N, Erakky A, Varghese AM, Zhang A, Kendall J, Ghiban E, Chorbadjiev L, Wu J, Dimitrova N, Chadalavada K, Nanjangud GJ, Bandlamudi C, Gong Y, Donoghue MTA, Socci ND, Krasnitz A, Notta F, Leach SD, Iacobuzio-Donahue CA, Lowe SW
Nature. 2022 Aug;608(7924):795-802. doi: 10.1038/s41586-022-05082-5

RNA Research

MED9 Expression Level as a Biomarker of Epithelial Ovarian Cancer Progression and Prognosis
Han GH, Yun H, Chung JY, Kim JH, Cho H
Cancer Genomics Proteomics. 2022 Nov-Dec;19(6):692-702. doi: 10.21873/cgp.20352

An mTORC1-mediated negative feedback loop constrains amino acid-induced FLCN-Rag activation in renal cells with TSC2 loss
Asrani K, Woo J, Mendes AA, Schaffer E, Vidotto T, Villanueva CR, Feng K, Oliveira L, Murali S, Liu HB, Salles DC, Lam B, Argani P, Lotan TL
Nat Commun. 2022 Nov 10;13(1):6808. doi: 10.1038/s41467-022-34617-7.

High-throughput sequencing of single neuron projections reveals spatial organization in the olfactory cortex
Chen Y, Chen X, Baserdem B, Zhan H, Li Y, Davis MB, Kebschull JM, Zador AM, Koulakov AA, Albeanu DF
Cell. 2022 Oct 27;185(22):4117-4134.e28. doi: 10.1016/j.cell.2022.09.038

Proteomics, Lipidomics, Metabolomics

Investigating pathological epigenetic aberrations by epi-proteomics
Robusti G, Vai A, Bonaldi T, Noberini R
Clin Epigenetics. 2022 Nov 12;14(1):145. doi: 10.1186/s13148-022-01371-y.

Connecting multiple microenvironment proteomes uncovers the biology in head and neck cancer
Busso-Lopes AF, Neves LX, Câmara GA, Granato DC, Pretti MAM, Heberle H, Patroni FMS, Sá J, Yokoo S, Rivera C, Domingues RR, Normando AGC, De Rossi T, Mello BP, Galdino NAL, Pauletti BA, Lacerda PA, Rodrigues AAN, Casarim ALM, de Lima-Souza RA, Damas II, Mariano FV, Gollob KJ, Medina TS, Cervigne NK, Prado-Ribeiro AC, Brandão TB, Villa LL, Uno M, Boroni M, Kowalski LP, González-Arriagada WA, Paes Leme AF:
Nat Commun. 2022 Nov 7;13(1):6725. doi: 10.1038/s41467-022-34407-1.

Comparative proteomic analysis of glomerular proteins in IgA nephropathy and IgA vasculitis with nephritis
Kaga H, Matsumura H, Saito A, Saito M, Abe F, Suzuki T, Dohmae N, Odaka M, Komatsuda A, Wakui H, Takahashi N:
Research Square, 28th Oct 2022

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
Mund A, Coscia F, Kriston A, Hollandi R, Kovacs F, Brunner A-D, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
Nat Biotechnol (2022). doi.org/10.1038/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Fluorescence Applications

Glial scar survives until the chronic phase by recruiting scar-forming astrocytes after spinal cord injury
Tamaru T, Kobayakawa K, Saiwai H, Konno D, Kijima K, Yoshizaki S, Hata K, Iura H, Ono G, Haruta Y, Kitade K, Iida KI, Kawaguchi KI, Matsumoto Y, Kubota K, Maeda T, Okada S, Nakashima Y:
Exp Neurol. 2022 Nov 3:114264. doi: 10.1016/j.expneurol.2022.114264.

Direct Reprogramming of Mouse Subchondral Bone Osteoblasts into Chondrocyte-Like Cells
Li M, Zhang L, Li J, and Zhu Q
Biomedicines 2022, 10, 2582.

Target formation in muscle fibres indicates reinnervation - a proteomic study in muscle samples from peripheral neuropathies
Krause K, Eggers B, Uszkoreit J, Eulitz S, Rehmann R, Güttsches AK, Schreiner A, van der Ven PFM, Fürst DO, Marcus K, Vorgerd M, Kley RA
Neuropathol Appl Neurobiol. 2022 Sep 30:e12853. doi: 10.1111/nan.12853.

Cancer Research

MED9 Expression Level as a Biomarker of Epithelial Ovarian Cancer Progression and Prognosis
Han GH, Yun H, Chung JY, Kim JH, Cho H
Cancer Genomics Proteomics. 2022 Nov-Dec;19(6):692-702. doi: 10.21873/cgp.20352.

Spatial genomics maps the structure, nature and evolution of cancer clones
Lomakin A, Svedlund J, Strell C, Gataric M, Shmatko A, Rukhovich G, Park JS, Ju YS, Dentro S, Kleshchevnikov V, Vaskivskyi V, Li T, Bayraktar OA, Pinder S, Richardson AL, Santagata S, Campbell PJ, Russnes H, Gerstung M, Nilsson M, Yates LR:
Nature. 2022 Nov 9. doi: 10.1038/s41586-022-05425-2.

Connecting multiple microenvironment proteomes uncovers the biology in head and neck cancer
Busso-Lopes AF, Neves LX, Câmara GA, Granato DC, Pretti MAM, Heberle H, Patroni FMS, Sá J, Yokoo S, Rivera C, Domingues RR, Normando AGC, De Rossi T, Mello BP, Galdino NAL, Pauletti BA, Lacerda PA, Rodrigues AAN, Casarim ALM, de Lima-Souza RA, Damas II, Mariano FV, Gollob KJ, Medina TS, Cervigne NK, Prado-Ribeiro AC, Brandão TB, Villa LL, Uno M, Boroni M, Kowalski LP, González-Arriagada WA, Paes Leme AF
Nat Commun. 2022 Nov 7;13(1):6725. doi: 10.1038/s41467-022-34407-1.

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
Mund A, Coscia F, Kriston A, Hollandi R, Kovacs F, Brunner A-D, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
Nat Biotechnol (2022). doi.org/10.1038/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Neurosciences

Clusters of co-regulated proteins in brain cortex associate with fronto-temporal lobar degeneration
Bridel C, van Gils JHM, Miedema SSM, Hoozemans JJ, Pijnenburg YAL, Smit AB, Rozemuller AJM, Abeln S, Teunissen CE
Research Square, 11th November 2022

BDNF Spinal Overexpression after Spinal Cord Injury Partially Protects Soleus Neuromuscular Junction from Disintegration, Increasing VAChT and AChE Transcripts in Soleus but Not Tibialis Anterior Motoneurons
Głowacka A, Ji B, Szczepankiewicz AA, Skup M, Gajewska-Woźniak O:
Biomedicines. 2022 Nov 8;10(11):2851. doi: 10.3390/biomedicines10112851.

Habenula as a Possible Target for Treatment-Resistant Depression Phenotype in Wistar Kyoto Rats
Korlatowicz A, Pabian P, Solich J, Kolasa M, Latocha K, Dziedzicka-Wasylewska M, Faron-Górecka A
Mol Neurobiol. 2022 Nov 8. doi: 10.1007/s12035-022-03103-y.

Clinical/Pathology/Immunology Research Applications

VPAC1 and VPAC2 receptor deficiencies negatively influence pregnancy outcome through distinct and overlapping modulations of immune, trophoblast and vascular functions
Calo G, Hauk V, Vota D, Van C, Condro M, Gallino L, Ramhorst R, Waschek J, Leirós CP:
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2022, 166593, ISSN 0925-4439,

Habenula as a Possible Target for Treatment-Resistant Depression Phenotype in Wistar Kyoto Rats
Korlatowicz A, Pabian P, Solich J, Kolasa M, Latocha K, Dziedzicka-Wasylewska M, Faron-Górecka A:
Mol Neurobiol. 2022 Nov 8. doi: 10.1007/s12035-022-03103-y.

Comparative proteomic analysis of glomerular proteins in IgA nephropathy and IgA vasculitis with nephritis
Kaga H, Matsumura H, Saito A, Saito M, Abe F, Suzuki T, Dohmae N, Odaka M, Komatsuda A, Wakui H, Takahashi N
Research Square, 28th Oct 2022

Developmental Research, Epigenetics  & Aging

VPAC1 and VPAC2 receptor deficiencies negatively influence pregnancy outcome through distinct and overlapping modulations of immune, trophoblast and vascular functions
Calo G, Hauk V, Vota D, Van C, Condro M, Gallino L, Ramhorst R, Waschek J, Leirós CP
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2022, 166593, ISSN 0925-4439,

Direct Reprogramming of Mouse Subchondral Bone Osteoblasts into Chondrocyte-Like Cells
Li M, Zhang L, Li J, and Zhu Q
Biomedicines 2022, 10, 2582.

TGF-β3 induces lactate production in Sertoli cell through inhibiting Notch pathway
Xu Y, Jiang S, Hu Y, Zhang Q, Su W
Andrology. 2022 Sep 4. doi: 10.1111/andr.13288.

Plant Research

Auxin-induced WUSCHEL-RELATED HOMEOBOX13 Mediates Asymmetric Activity of Callus Formation upon Cutting
Tanaka H, Hashimoto N, Kawai S, Yumoto E, Shibata K, Tameshige T, Yamamoto Y, Sugimoto K, Asahina M, Ikeuchi M
Plant Cell Physiol. 2022 Oct 20:pcac146. doi: 10.1093/pcp/pcac146. 

RsCLE22a Regulates the Taproot Growth through Auxin Signaling-related Pathway in Radish (Raphanus sativus L.)
Dong J, Wang Y, Xu L, Li B, Wang K, Ying J, He Q, Liu L
J Exp Bot. 2022 Oct 14;erac406. doi: 10.1093/jxb/erac406.

The cellulose–lignin balance affects the twisted growth of Yunnan pine trunk
Cui K, Liu Z, Li J, Gao C, and Miao Y
Authorea Preprints, 10 Oct 2022, 

Forensics

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
Sun M, Zheng J, Wnag B and Yao J:
BioMed Research International, Volume 2021 | Article ID 7269237

Application and utility of alternative methods in isolation of pure cells from forensic biological mixtures in modern-day: a review
Prajapati S, Rajmane P, Jayakrishna P, Nair MS, Kshirsagar P and Meshram M:
JFSR – Journal of Forensic Science and Research, Aug 2021, ISSN 2575-0186

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Further information on Leica LMD impact at forensics: 
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Improved Spatial Resolution of Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI) Mass Spectrometry Imaging (MSI) Using a Reflective Objective
Joignant AN, Bai H, Manni JG Sr, Muddiman DC 
Rapid Commun Mass Spectrom. 2022 Sep 4:e9392. doi: 10.1002/rcm.9392.

Profiling cell-type specific gene expression in post-mortem human brain samples through laser capture microdissection
Almeida D, Turecki G
Methods. 2022 Sep 2:S1046-2023(22)00196-7. doi: 10.1016/j.ymeth.2022.08.013.

Hepatic cytochrome P450 8B1 and cholic acid potentiate intestinal epithelial injury in colitis by suppressing intestinal stem cell renewal
Chen L, Jiao T, Liu W, Luo Y, Wang J, Guo X, Tong X, Lin Z, Sun C, Wang K, He Y, Zhang Y, Xu H, Wang J, Zuo J, Ding Q, He S, Gonzalez FJ, Xie C
Cell Stem Cell. 2022 Sep 1;29(9):1366-1381.e9. doi: 10.1016/j.stem.2022.08.008.

Live Cell Culture

Direct Reprogramming of Mouse Subchondral Bone Osteoblasts into Chondrocyte-Like Cells
Li M, Zhang L, Li J, and Zhu Q
Biomedicines 2022, 10, 2582.

Heterotypic cell-in-cell structures between cancer and NK cells are associated with enhanced anticancer drug resistance
Choe YJ, Min JY, Lee H, Lee SY, Kwon J, Kim HJ, Lee J, Kim HM, Park HS, Cho MY, Hyun JY, Kim HM, Chung YH, Ha SK, Jeong HG, Choi I, Kim TD, Hong KS, and Han EH
iScience 25, 105017 September 16, 2022

Activation of CD44 signaling in leader cells induced by tumor-associated macrophages drives collective detachment in luminal breast carcinomas
Gao F, Zhang G, Liu Y, He Y, Sheng Y, Sun X, Du Y, Yang C
Cell Death Dis. 2022 Jun 9;13(6):540. doi: 10.1038/s41419-022-04986-4.

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