Leica LMD6 & LMD7 Microdissection laser

This curated list features recent scientific publications that have employed Leica’s LMD6 and LMD7 laser microdissection systems in key areas such as cancer research, neuroscience, molecular pathology, forensics, and spatial omics. It is updated regularly to reflect the latest scientific publications utilizing laser microdissection applications.

Explore the full list below to see how Leica LMD systems are enabling scientific success across the globe.

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Single Cell Applications

Pathogenic Role of SERPINB3-positive Neutrophils in Reinforcing Thrombus Stiffening during Ischemic Stroke
Zang J, Lu A, Yang B, Tan N, Liu Q, Wei L, Liang Y, Zhou S, Tan Z, Xin X, He S, Wu P, Li Y, Xu Z, Su X, Mai H, Xu A, Lu D
Blood. 2026 Mar 3:blood.2025029516. doi: 10.1182/blood.2025029516.

Single-cell spatial proteomics maps human liver zonation patterns and their vulnerability to disruption in tissue architecture
Weiss CAM, Brown LA, Miranda L, Pellizzoni P, Steigerwald S, Remmert K, Hernandez JM, Borgwardt K, Kleiner DE, Ben-Moshe S, Rosenberger FA, Porat-Shliom N, Mann M
Nat Metab. 2026 Feb 20. doi: 10.1038/s42255-026-01459-2.

Laser  Capture  Microdissection:  Methods  and  Protocols: Immunofluorescence  Imaging-Guided  Laser Microdissection  for  Ultralow  Input  Spatial  Tissue Proteomics
Fritzsche S, Coscia F in Roberta  Noberini  (ed.)
Methods  in  Molecular  Biology,  vol.  3015

Spatial Genomics (DNA-Reserach)

A disease model resource reveals core principles of tissue-specific cancer evolution
Mueller S, de Andrade Krätzig N, Tschurtschenthaler M, Silva MG, Thordsen C, Trozzo R, Simon P, Saab F, Kaltenbacher T, Zukowska M, Lucarelli D, Öllinger R, Griger J, Groß N, Groll T, Löprich J, Zaurito AE, Schömig LR, Bugter JM, Bärthel S, Falcomatà C, Strong A, Brandt C, Najajreh M, Papargyriou A, Maresch R, Collins KAN, Sailer D, Schneeweis C, Burger S, Fröhlich LM, Klement C, Belka A, Montero JJ, Jungwirth U, Reichert M, Moser M, Neumann J, Vassiliou G, Cadiñanos J, Varela I, Marr C, Alonso DF, Lollini PL, Zhao J, Chesler L, Isacke CM, Riedel A, Braun CJ, Sos ML, Beleggia F, Reinhardt HC, Musteanu M, Barbacid M, Quante M, Schmidt-Supprian M, Schneider G, Clare S, Lawley TD, Dougan G, Steiger K, Conte N, Bradley A, Rad L, Saur D, Rad R
Nature. 2026 Feb 25. doi: 10.1038/s41586-026-10187-2.2

Identification of the Carcinogenic Process from Lobular Endocervical Glandular Hyperplasia to Gastric-Type Adenocarcinoma of the Uterine Cervix via Whole-Exome Sequencing
Kuruma A, Masuda T, Sato K, Kido K, Motooka D, Komura N, Yokoi T, Yoshihara K, Kinose Y, Hashimoto K, Sawada K, Morii E, Kimura T and Kodama M
Cancers2026, 18(4), 651; doi.org/10.3390/cancers18040651

Laser  Capture  Microdissection:  Methods  and  Protocols: Laser  Capture  Microdissection  from  Blood  Smears
Matsuo H in Roberta  Noberini  (ed.)
Methods  in  Molecular  Biology,  vol.  3015

Spatial Transcriptomics (RNA Research)

Possible involvement of tubular interleukin-34 in macrophage recruitment during colistin-induced nephrotoxicity in rats
Matsushita K, Akane H, Akagi JI, Morikawa T, Mizuta Y, Ogawa K, Toyoda T
J Toxicol Sci. 2026;51(3):163-172. doi: 10.2131/jts.51.163.

Cardiac conduction system malformations in heterotaxy result from dysregulated Pitx2 expression
Joo K, Matsuoka R, Kitajima K, Yashiro K, Shiose A, Tominaga R, Shen MM, Oki S, Meno C
JCI Insight. 2026 Feb 24:e199072. doi: 10.1172/jci.insight.199072

Fetal inflammatory signals regulate maternal investment during marsupial pregnancy
Stadtmauer DJ, Maziarz JD, Griffith OW, Wagner GP
PLoS Biol. 2026 Feb 24;24(2):e3003670. doi: 10.1371/journal.pbio.3003670.

Proteomics, Lipidomics, Metabolomics

Pan-cancer multi-omic ERBB2-HER2 characterization using next-generation sequencing and quantitative proteomics
Hunt AL, Randall J, Ogata JD, Johnston L, Swain W, Melvin S, Sharma M, Calvert V, Maxwell GL, Bateman NW, Petricoin EF, Conrads TP, Cannon TL
NPJ Precis Oncol. 2026 Mar 5. doi: 10.1038/s41698-026-01351-y.

Pathogenic Role of SERPINB3-positive Neutrophils in Reinforcing Thrombus Stiffening during Ischemic Stroke
Zang J, Lu A, Yang B, Tan N, Liu Q, Wei L, Liang Y, Zhou S, Tan Z, Xin X, He S, Wu P, Li Y, Xu Z, Su X, Mai H, Xu A, Lu D
Blood. 2026 Mar 3:blood.2025029516. doi: 10.1182/blood.2025029516.

Spatial Profiling of Glycosaminoglycans (GAGomics) from Laser Microdissected Mouse Brain
Mernie E, Zaia J
Mol Cell Proteomics. 2026 Feb 19:101533. doi: 10.1016/j.mcpro.2026.101533.

Deep Visual Proteomics (DVP) related

Single-cell spatial proteomics maps human liver zonation patterns and their vulnerability to disruption in tissue architecture
Weiss CAM, Brown LA, Miranda L, Pellizzoni P, Steigerwald S, Remmert K, Hernandez JM, Borgwardt K, Kleiner DE, Ben-Moshe S, Rosenberger FA, Porat-Shliom N, Mann M
Nat Metab. 2026 Feb 20. doi: 10.1038/s42255-026-01459-2.

Spatial protein gradients pattern liver metabolism
Dong Z, Guo T
Nat Metab (2026). doi.org/10.1038/s42255-026-01478-z

Laser  Capture  Microdissection:  Methods  and  Protocols: Immunofluorescence  Imaging-Guided  Laser Microdissection  for  Ultralow  Input  Spatial  Tissue Proteomics
Fritzsche S, Coscia F in Roberta  Noberini  (ed.)
Methods  in  Molecular  Biology,  vol.  3015

Fluorescence Applications

Pathogenic Role of SERPINB3-positive Neutrophils in Reinforcing Thrombus Stiffening during Ischemic Stroke
Zang J, Lu A, Yang B, Tan N, Liu Q, Wei L, Liang Y, Zhou S, Tan Z, Xin X, He S, Wu P, Li Y, Xu Z, Su X, Mai H, Xu A, Lu D
Blood. 2026 Mar 3:blood.2025029516. doi: 10.1182/blood.2025029516.

Laser  Capture  Microdissection:  Methods  and  Protocols: A  Spatial  Proteomics  Approach  to  Isolate  and  Analyze Amyloid  Proteins  Using  Laser  Capture  Microdissection and  Mass  Spectrometry
Aguilan J, Horton M, Pessin JE, and Sidoli S in Roberta  Noberini  (ed.)
Methods  in  Molecular  Biology,  vol.  3015

Separation and identification of two-individual mixed bloodstains using human neutrophil antigen-1 antibodies
Xia X, Zheng JL, Wang SW, Liu Z, Li Z, Wang DY, Li XN, Wu X, Yao J
Int J Legal Med. 2026 Jan 27. doi: 10.1007/s00414-026-03718-z.

Cancer Research

Pan-cancer multi-omic ERBB2-HER2 characterization using next-generation sequencing and quantitative proteomics
Hunt AL, Randall J, Ogata JD, Johnston L, Swain W, Melvin S, Sharma M, Calvert V, Maxwell GL, Bateman NW, Petricoin EF, Conrads TP, Cannon TL
NPJ Precis Oncol. 2026 Mar 5. doi: 10.1038/s41698-026-01351-y.

A disease model resource reveals core principles of tissue-specific cancer evolution
Mueller S, de Andrade Krätzig N, Tschurtschenthaler M, Silva MG, Thordsen C, Trozzo R, Simon P, Saab F, Kaltenbacher T, Zukowska M, Lucarelli D, Öllinger R, Griger J, Groß N, Groll T, Löprich J, Zaurito AE, Schömig LR, Bugter JM, Bärthel S, Falcomatà C, Strong A, Brandt C, Najajreh M, Papargyriou A, Maresch R, Collins KAN, Sailer D, Schneeweis C, Burger S, Fröhlich LM, Klement C, Belka A, Montero JJ, Jungwirth U, Reichert M, Moser M, Neumann J, Vassiliou G, Cadiñanos J, Varela I, Marr C, Alonso DF, Lollini PL, Zhao J, Chesler L, Isacke CM, Riedel A, Braun CJ, Sos ML, Beleggia F, Reinhardt HC, Musteanu M, Barbacid M, Quante M, Schmidt-Supprian M, Schneider G, Clare S, Lawley TD, Dougan G, Steiger K, Conte N, Bradley A, Rad L, Saur D, Rad R
Nature. 2026 Feb 25. doi: 10.1038/s41586-026-10187-2.2

Identification of the Carcinogenic Process from Lobular Endocervical Glandular Hyperplasia to Gastric-Type Adenocarcinoma of the Uterine Cervix via Whole-Exome Sequencing
Kuruma A, Masuda T, Sato K, Kido K, Motooka D, Komura N, Yokoi T, Yoshihara K, Kinose Y, Hashimoto K, Sawada K, Morii E, Kimura T and Kodama M
Cancers2026, 18(4), 651; doi.org/10.3390/cancers18040651

Neurosciences

Human brain prefrontal cortex proteomics identifies compromised energy metabolism and neuronal function in Schizophrenia
Koopmans F, Dijkstra AA, Li WP, Klaassen RV, Gouwenberg Y, Yao S, Bast L, Verhage M, Karlsson R, Dwork AJ, Stockmeier CA, Hjerling-Leffler J, Sullivan PF, Smit AB
Nat Commun. 2026 Jan 29. doi: 10.1038/s41467-026-68950-y.

Proteomic remodelling of the neurobrillary tangle from “PART” to advanced Alzheimer’s disease
Thierry M, Kavanagh T, Balcomb K, Tang L, Leitner D, Kanshin E, William C, Oakley D, Hyman B, Ueberheide B, Drummond E, Wisniewski T
Research Square, 29th Jan 2026

Neural crest cell-derived DKK1 and NEDD4 modulate Wnt signalling in the second heart field to orchestrate outflow tract development
Wiszniak S, Alankarage D, Lohraseb I, Marchant C, Secker G, Domingo D, Hartmann J, Zhang T, Parker W, Toubia J, White M, Piltz S, Tondl M, Giannoulatou E, Winlaw D, Blue GM; Congenital Heart Disease Synergy Group; Tam PPL, Thomas P, Harvey N, Dunwoodie SL, Schwarz Q
Nat Commun. 2026 Jan 22. doi: 10.1038/s41467-026-68459-4.

Clinical/Pathology/Immunology Research Applications

Possible involvement of tubular interleukin-34 in macrophage recruitment during colistin-induced nephrotoxicity in rats
Matsushita K, Akane H, Akagi JI, Morikawa T, Mizuta Y, Ogawa K, Toyoda T
J Toxicol Sci. 2026;51(3):163-172. doi: 10.2131/jts.51.163.

Cardiac conduction system malformations in heterotaxy result from dysregulated Pitx2 expression
Joo K, Matsuoka R, Kitajima K, Yashiro K, Shiose A, Tominaga R, Shen MM, Oki S, Meno C
JCI Insight. 2026 Feb 24:e199072. doi: 10.1172/jci.insight.199072

Single-cell spatial proteomics maps human liver zonation patterns and their vulnerability to disruption in tissue architecture
Weiss CAM, Brown LA, Miranda L, Pellizzoni P, Steigerwald S, Remmert K, Hernandez JM, Borgwardt K, Kleiner DE, Ben-Moshe S, Rosenberger FA, Porat-Shliom N, Mann M
Nat Metab. 2026 Feb 20. doi: 10.1038/s42255-026-01459-2.

Developmental Research, Epigenetics & Aging

Fetal inflammatory signals regulate maternal investment during marsupial pregnancy
Stadtmauer DJ, Maziarz JD, Griffith OW, Wagner GP
PLoS Biol. 2026 Feb 24;24(2):e3003670. doi: 10.1371/journal.pbio.3003670.

A high-quality RNA-yielding protocol for laser capture microdissection of transplanted stem cell-derived Islets of Langerhans
Norman D, Lau J
Acta Diabetol. 2026 Feb 9. doi: 10.1007/s00592-026-02654-z.

Comparative insights into molecular pathways influencing germline development in early-divergent angiosperms
Lora J, Tucker MR, Shirley NJ, Ma C, Hormaza JI
Plant J. 2026 Jan;125(2):e70675. doi: 10.1111/tpj.70675.

Plant Research

Comparative insights into molecular pathways influencing germline development in early-divergent angiosperms
Lora J, Tucker MR, Shirley NJ, Ma C, Hormaza JI
Plant J. 2026 Jan;125(2):e70675. doi: 10.1111/tpj.70675.

Gene networks associated with early endosperm proliferation and basal endosperm layer differentiation in maize
Zhang S, Ran D, Ryu CH, Li G, Dannenhoffer JM, Wang X, Drews GN, and Yadegari R
bioRxiv, Jan 2nd 2026

Single-cell and spatial omics reveal progressive loss of xylem developmental complexity across seed plants
Shuai P, Hsieh JA, Kao CT, Hu CW, Wang R, Kuo SC, Yen MR, Liou PC, Ho YC, Chu CC, Huang S, Liu J, Zhang L, Wu CC, Luo YJ, Li Q, Hsu CC, Huang CL, Su JC, Tseng MC, Chen YL, Mai TL, Lin YJ
Plant Cell. 2025 Oct 19:koaf253. doi: 10.1093/plcell/koaf253.

Forensics

Separation and identification of two-individual mixed bloodstains using human neutrophil antigen-1 antibodies
Xia X, Zheng JL, Wang SW, Liu Z, Li Z, Wang DY, Li XN, Wu X, Yao J
Int J Legal Med. 2026 Jan 27. doi: 10.1007/s00414-026-03718-z.

Invisible WItness: Innovations in the Collection of Touch DNA and the Risk of Forensic Corss Contamination
Irfan M, Haider SA, Yasir M, Akram MA, Adeel M
FORENSIC INSIGHTS AND HEALTH SCIENCES BULLETIN, Volume 3: Issue 2: 2025

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Miscellaneous

Evaluation of the specifi city of phage antibodies obtained from canine breast tumors by fl ow cytometry and fl uorescence microscopy
Kozlov ES, Staroverov SA, Kruchinina VS, Salautin VV, Dykman LA
Agrarian Scientifi c Journal. 2026;(3):74–78

Spatial Profiling of Glycosaminoglycans (GAGomics) from Laser Microdissected Mouse Brain
Mernie E, Zaia J
Mol Cell Proteomics. 2026 Feb 19:101533. doi: 10.1016/j.mcpro.2026.101533.

Spatial Proteomics Using S4P
Qin R, He F, Qin 
Bio-protocol 16(5): e5620. DOI: 10.21769/BioProtoc.5620.

Live Cell Culture

Muscle Structure and Function Recovery: Adalimumab-Calcium Channel Synergy in Post-Ischemic Stroke Sarcopenia
Qi H, Zhang XW, Zhang ZY, Ou SS, Gao YL, Tian D, Jiang YN, Min XR, Zhao AT, Zou JM, Zeng JS, Pu QY, Yang RC, Zeng N
J Cachexia Sarcopenia Muscle. 2025 Dec;16(6):e70097. doi: 10.1002/jcsm.70097.

Ultrasensitive proteomics uncovers nociceptor diversity and novel pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
bioRxiv, Sept 21, 2025

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

Laser microdissection (LMD) is an advanced, highly precise sample preparation technique that enables researchers to isolate pure, defined regions of interest, including single cells, from complex tissue samples. The method ensures reproducibility and accuracy for downstream analyses such as Next Generation Sequencing (NGS) including RNA-seq, and mass spectrometry for spatial genomics, spatial transcriptomics, spatial proteomics & spatial multiomics.

LMD allows non-contact dissection, isolation and gravity-assisted collection of targeted single cells, tissue compartments, cell clusters and even subcellular structures under visual control. These systems uniquely bridge microscopy and molecular biology to unlock true spatial omics insights. Combined with software-guided segmentation, Leica LMD systems enable high throughput workflows for biomarker discovery and identification of therapeutical interaction points. The study by Mund & Coscia et al., describing the Deep Visual Proteomics (DVP) workflow powered by the Leica LMD7 system, was featured on the cover of Nature Biotechnology in August 2022.  Follow-up research by Nordmann et al., published in Nature in 2024, demonstrated how DVP contributed to a life-saving therapy for toxic epidermal necrolysis (TEN), a deadly skin disease. These breakthroughs also contributed to the selection of “spatial proteomics” as Nature’s Method of the Year 2024.