Leica LMD6 & LMD7 Microdissection laser
La dissection parfaite
This list presents current publications in the major application fields for laser microdissection and is updated monthly.
Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.
The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.
Now there is single-cell Deep Visual Proteomics (scDVP), a technology that integrates high-content imaging, laser microdissection, and multiplexed mass spectrometry (MS) for tissue characterization at the single cell level.
Download the complete list of LMD publications
Single cell applications
An automated and fast sample preparation workflow for laser microdissection guided ultrasensitive proteomics
Makhmut A, Qin D, Hartlmayr D, Seth A, and Coscia F
bioRxiv, Dec 1st 2023
Gene expression in the primate orbitofrontal cortex related to anxious temperament
Kenwood MM, Souaiaia T, Kovner R, Fox AS, French DA, Oler JA, Roseboom PH, Riedel MK, Mueller SAL, Kalin NH
Proc Natl Acad Sci U S A. 2023 Dec 5;120(49):e2305775120. doi: 10.1073/pnas.2305775120.
Research progress and application of single-cell sequencing in head and neck malignant tumors
Qu S, Gong M, Deng Y, Xiang Y, Ye D
Cancer Gene Ther. 2023 Nov 15. doi: 10.1038/s41417-023-00691-2.
DNA Research
DEEP MOLECULAR TRACKING OVER THE 12-YEAR DEVELOPMENT OF ENDOMETRIAL CANCER FROM HYPERPLASIA IN A SINGLE PATIENT - Endometrial hyperplasia to cancer
Reid K, Camacho-Vanegas O, Pandya D, Camacho SC, Qiao RF, Kalir T, Padron MM, Beddoe A-M, Dottino P, and Martignetti JA
Cold Spring Harbor Laboratory Press, Oct 30th 2023, from molecularcasestudies.cshlp.org
Strategic approach to heterogeneity analysis of cutaneous adnexal carcinomas using computational pathology and genomics
Nishimura T, Eijitsu R, Inoue S, Kawazu M, Ueno T, Namikawa K, Takahashi A, OgataD, Yoshida A, Yamazaki N, Mano H, Yatabe Y, Mori T
JID Innovations, Skin Science from Molecules to Population Health, 05 Sep 2023
Molecular biological analysis revealed a case of synchronous endometrial and ovarian cancer with different histological grade as metastatic ovarian cancer from endometrial cancer: Case report and review of literature
Yumisashi R, Saito R, Togami S, Kobayashi Y, Kitazono I, Tanimoto A, Kobayashi H
J Obstet Gynaecol Res. 2023 Aug 21. doi: 10.1111/jog.15773.
RNA Research
Transcriptome profile of subsynaptic myonuclei at the neuromuscular junction in embryogenesis
Ohkawara B, Kurokawa M, Kanai A, Imamura K, Chen G, Zhang R, Masuda A, Higashi K, Mori H, Suzuki Y, Kurokawa K, Ohno K
J Neurochem. 2023 Nov 23. doi: 10.1111/jnc.16013.
Uterine scars after caesarean delivery: From histology to the molecular and ultrastructural level
Paping A, Basler C, Ehrlich L, Fasting C, Melchior K, Ziska T, Thiele M, Duda GN, Timm S, Ochs M, Rancourt RC, Henrich W, Braun T
Wound Repair Regen. 2023 Nov 13. doi: 10.1111/wrr.13127.
Angiotensin II type 1 receptor-associated protein deletion combined with angiotensin II stimulation accelerates the development of diabetic kidney disease in mice on a C57BL/6 strain
Taguchi S, Azushima K, Yamaji T, Suzuki T, Abe E, Tanaka S, Hirota K, Tsukamoto S, Morita R, Kobayashi R, Kinguchi S, Yamashita A, Wakui H, Tamura K
Hypertens Res. 2023 Nov 13. doi: 10.1038/s41440-023-01496-4.
Highlights 2023: SPARCS (spatially resolved CRISPR screening)
Schmacke et al.
https://doi.org/10.1101/2023.06.01.542416
Proteomics, Lipidomics, Metabolomics
An automated and fast sample preparation workflow for laser microdissection guided ultrasensitive proteomics
Makhmut A, Qin D, Hartlmayr D, Seth A, and Coscia F
bioRxiv, Dec 1st 2023
Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Krishnan R, Shimizu A, Metousis A, Kenny H, Mendoza R, Nordmann TM, Rauch S, Kelliher L, Heide J, Rosenberger FA, Bilecz A, Borrego SN, Strauss MT, Thielert M, Rodriguez E, Müller-Reif, JB, Chen M, Yamada SD, Mund A, Lastra RR, Mann M, and Lengyei E
medRxiv preprint, November 13, 2023
Single-cell spatial proteomics identifies the JAK/STAT pathway as an actionable therapeutic target in lethal cutaneous drug reactions
Nordmann TM, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler P-C, Sinha A, Metousis A, Rosenberger FA, Zwiebel M, Satoh TK, Anzengruber F, Tanzer MC, Saito Y, Gong T, Thielert M, Kimura H, Silke N, Rodriguez EH, Gaetana R, Nguyen HH, Gross A, Levesque MP, Murray PJ, Ingen-Housz-Oro S, Mund A, Abe R, Silke J, Ji C, French LE, Mann M
edRxiv preprint, November 12, 2023
Highlight 2022: Deep Visual Proteomics (DVP)
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/
Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409
Fluorescence Applications
An automated and fast sample preparation workflow for laser microdissection guided ultrasensitive proteomics
Makhmut A, Qin D, Hartlmayr D, Seth A, and Coscia F
bioRxiv, Dec 1st 2023
Gene expression in the primate orbitofrontal cortex related to anxious temperament
Kenwood MM, Souaiaia T, Kovner R, Fox AS, French DA, Oler JA, Roseboom PH, Riedel MK, Mueller SAL, Kalin NH
Proc Natl Acad Sci U S A. 2023 Dec 5;120(49):e2305775120. doi: 10.1073/pnas.2305775120.
Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Krishnan R, Shimizu A, Metousis A, Kenny H, Mendoza R, Nordmann TM, Rauch S, Kelliher L, Heide J, Rosenberger FA, Bilecz A, Borrego SN, Strauss MT, Thielert M, Rodriguez E, Müller-Reif, JB, Chen M, Yamada SD, Mund A, Lastra RR, Mann M, and Lengyei E
medRxiv preprint, November 13, 2023
Cancer Research
Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Krishnan R, Shimizu A, Metousis A, Kenny H, Mendoza R, Nordmann TM, Rauch S, Kelliher L, Heide J, Rosenberger FA, Bilecz A, Borrego SN, Strauss MT, Thielert M, Rodriguez E, Müller-Reif, JB, Chen M, Yamada SD, Mund A, Lastra RR, Mann M, and Lengyei E
medRxiv preprint, November 13, 2023
In situ profiling reveals metabolic alterations in the tumor microenvironment of ovarian cancer after chemotherapy
Corvigno S, Badal S, Spradlin ML, Keating M, Pereira I, Stur E, Bayraktar E, Foster KI, Bateman NW, Barakat W, Darcy KM, Conrads TP, Maxwell GL, Lorenzi PL, Lutgendorf SK, Wen Y, Zhao L, Thaker PH, Goodheart MJ, Liu J, Fleming N, Lee S, Eberlin LS, Sood AK
NPJ Precis Oncol. 2023 Nov 3;7(1):115. doi: 10.1038/s41698-023-00454-0.
The murine metastatic microenvironment of experimental brain metastases of breast cancer differs by host age in vivo: a proteomic study
Hunt AL, Khan I, Wu AML, Makohon-Moore SC, Hood BL, Conrads KA, Abulez T, Ogata J, Mitchell D, Gist G, Oliver J, Wei D, Chung MA, Rahman S, Bateman NW, Zhang W, Conrads TP, Steeg PS
Clin Exp Metastasis. 2023 Nov 2. doi: 10.1007/s10585-023-10233-7.
Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
Mund A, Coscia F, Kriston A, Hollandi R, Kovacs F, Brunner A-D, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
Nat Biotechnol (2022). doi.org/10.1038/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8
Neurosciences
Gene expression in the primate orbitofrontal cortex related to anxious temperament
Kenwood MM, Souaiaia T, Kovner R, Fox AS, French DA, Oler JA, Roseboom PH, Riedel MK, Mueller SAL, Kalin NH
Proc Natl Acad Sci U S A. 2023 Dec 5;120(49):e2305775120. doi: 10.1073/pnas.2305775120.
Transcriptome profile of subsynaptic myonuclei at the neuromuscular junction in embryogenesis
Ohkawara B, Kurokawa M, Kanai A, Imamura K, Chen G, Zhang R, Masuda A, Higashi K, Mori H, Suzuki Y, Kurokawa K, Ohno K
J Neurochem. 2023 Nov 23. doi: 10.1111/jnc.16013.
NR1 Splicing Variant NR1a in Cerebellar Granule Neurons Constitutes a Better Motor Learning in the Mouse
Tan T, Jiang L, He Z, Ding X, Xiong X, Tang M, Chen Y, Tang Y
Cerebellum. 2023 Oct 25. doi: 10.1007/s12311-023-01614-5.
Clinical/Pathology/Immunology Research Applications
Angiotensin II type 1 receptor-associated protein deletion combined with angiotensin II stimulation accelerates the development of diabetic kidney disease in mice on a C57BL/6 strain
Taguchi S, Azushima K, Yamaji T, Suzuki T, Abe E, Tanaka S, Hirota K, Tsukamoto S, Morita R, Kobayashi R, Kinguchi S, Yamashita A, Wakui H, Tamura K
Hypertens Res. 2023 Nov 13. doi: 10.1038/s41440-023-01496-4.
Single-cell spatial proteomics identifies the JAK/STAT pathway as an actionable therapeutic target in lethal cutaneous drug reactions
Nordmann TM, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler P-C, Sinha A, Metousis A, Rosenberger FA, Zwiebel M, Satoh TK, Anzengruber F, Tanzer MC, Saito Y, Gong T, Thielert M, Kimura H, Silke N, Rodriguez EH, Gaetana R, Nguyen HH, Gross A, Levesque MP, Murray PJ, Ingen-Housz-Oro S, Mund A, Abe R, Silke J, Ji C, French LE, Mann M
medRxiv preprint, November 12, 2023
An extra-erythrocyte role of haemoglobin body in chondrocyte hypoxia adaption
Zhang F, Zhang B, Wang Y, Jiang R, Liu J, Wei Y, Gao X, Zhu Y, Wang X, Sun M, Kang J, Liu Y, You G, Wei D, Xin J, Bao J, Wang M, Gu Y, Wang Z, Ye J, Guo S, Huang H, Sun Q
Nature. 2023 Oct 4. doi: 10.1038/s41586-023-06611-6.
Developmental Research, Epigenetics & Aging
Transcriptome profile of subsynaptic myonuclei at the neuromuscular junction in embryogenesis
Ohkawara B, Kurokawa M, Kanai A, Imamura K, Chen G, Zhang R, Masuda A, Higashi K, Mori H, Suzuki Y, Kurokawa K, Ohno K
J Neurochem. 2023 Nov 23. doi: 10.1111/jnc.16013.
Protein reservoirs of seeds are amyloid composites employed differentially for germination and seedling emergence
Sinha N, Zahra T, Gahane AY, Rout B, Bhattacharya A, Basu S, Chakrabarti A, Thakur AK
Plant J. 2023 Oct;116(2):329-346. doi: 10.1111/tpj.16429.
Mast Cell Dynamics in the Ovary Are Governed by GnRH and Prolactin
Laoharatchatathanin T, Rieanrakwong D, Hatsugai Y, Terashima R, Yonezawa T, Kurusu S, Kawaminami M
Endocrinology. 2023 Sep 23;164(11):bqad144. doi: 10.1210/endocr/bqad144.
Plant Research
Laser capture microdissection transcriptome (LCM RNAseq) reveals BcDFR is a key gene in anthocyanin synthesis of Non-heading Chinese cabbage
Zhou Q, Xu X, Li M, Yang X, Wang M, Li Y, Liu T, Hou X
Research Square, 28th Nov 2023
Genome-wide identification of Avicennia marina aquaporins reveals their role in adaptation to intertidal habitats and their relevance to salt secretion and vivipary
Guo Z, Wei M, Xu C, Wang L, Li J, Liu J, Zhong Y, Chi B, Song S, Zhang L, Song L, Ma D, Zheng HL
Plant Cell Environ. 2023 Nov 20. doi: 10.1111/pce.14769.
Protein reservoirs of seeds are amyloid composites employed differentially for germination and seedling emergence
Sinha N, Zahra T, Gahane AY, Rout B, Bhattacharya A, Basu S, Chakrabarti A, Thakur AK
Plant J. 2023 Oct;116(2):329-346. doi: 10.1111/tpj.16429.
Forensics
Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807
Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136
Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
Sun M, Zheng J, Wnag B and Yao J:
BioMed Research International, Volume 2021 | Article ID 7269237
General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/
Further information on Leica LMD impact at forensics:
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/
Miscellaneous
An automated and fast sample preparation workflow for laser microdissection guided ultrasensitive proteomics
Makhmut A, Qin D, Hartlmayr D, Seth A, and Coscia F
bioRxiv, Dec 1st 2023
Research progress and application of single-cell sequencing in head and neck malignant tumors
Qu S, Gong M, Deng Y, Xiang Y, Ye D
Cancer Gene Ther. 2023 Nov 15. doi: 10.1038/s41417-023-00691-2.
Routine Workflow of Spatial Proteomics on Micro-formalin-Fixed Paraffin-Embedded Tissues
Chen H, Zhang Y, Zhou H, Chen W, Peng J, Feng Y, Fan L, Li J, Zi J, Ren Y, Li Q, Liu S
Anal Chem. 2023 Nov 3. doi: 10.1021/acs.analchem.3c03848.
Live Cell Culture
A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023
SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416
An efficient semi–in vivo zygotic embryogenesis system in Arabidopsis
Li J, Li SS, Liu Y, Tang WY, Liu XF, Meng CF, Zhang LY, Sun MX
In Vitro Cell.Dev.Biol.-Plant, 27. Dec 2022
Intéressé(e) d'en savoir plus ?
Parlez à notre expert.
Préférez-vous un conseil personnalisé ? Show local contacts