Leica LMD6 & LMD7 Microdissection laser

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

Analysis of microisolated frontal cortex excitatory layer III and V pyramidal neurons reveals a neurodegenerative phenotype in individuals with Down syndrome
Alldred MJ, Pidikiti H, Ibrahim KW, Lee SH, Heguy A, Hoffman GE, Roussos P, Wisniewski T, Wegiel J, Stutzmann GE, Mufson EJ, Ginsberg SD
Acta Neuropathol. 2024 Aug 6;148(1):16. doi: 10.1007/s00401-024-02768-0.

Deciphering functional tumor–immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann M
bioRxiv preprint, May 23, 2024

The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma
Hu H, Liu G, Li Y
Plant Methods. 2024 May 17;20(1):72. doi: 10.1186/s13007-024-01184-5.

DNA Research

Differences in BRAF V600E mutation between the epithelium and mesenchyme in classic ameloblastoma
Chen Z, Hong Y, Zhao Z, Wu N, Ma X, Chen L, Zhang R 
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 21 Aug 2024

Identification of microbial antigens in liver tissues involved in the pathogenesis of primary biliary cholangitis using 16S rRNA metagenome analysis
Katsumi T, Sato H, Murakami R, Hanatani T, Uchiyama F, Suzuki F, Maki K, Hoshikawa K, Haga H, Saito T, Ueno Y
PLoS One. 2024 Aug 19;19(8):e0308912. doi: 10.1371/journal.pone.0308912.

Oral pimonidazole unveils clinicopathologic and epigenetic features of hypoxic tumour aggressiveness in localized prostate cancer
Ci X, Chen S, Zhu R, Zarif M, Jain R, Guo W, Ramotar M, Gong L, Xu W, Singh O, Mansouri S, Zadeh G, Wei GH, Xu W, Bristow R, Berlin A, Koritzinsky M, van der Kwast T, He HH
BMC Cancer. 2024 Jun 18;24(1):744. doi: 10.1186/s12885-024-12505-1.

RNA Research

Collagen-Heparin-FGF2-VEGF Scaffolds Induce a Regenerative Gene Expression Profile in a Fetal Sheep Wound Model
Gansevoort M, Oostendorp C, Bouwman LF, Tiemessen DM, Geutjes PJ, Feitz WFJ, van Kuppevelt TH, Daamen WF
Tissue Eng Regen Med. 2024 Aug 31. doi: 10.1007/s13770-024-00667-9.

Intra-patient spatial comparison of non-metastatic and metastatic lymph nodes reveals a reduction in CD169+ macrophages within metastatic breast cancers
Maeshima Y, Kataoka TR, Vandenbon A, Hirata M, Takeuchi Y, Suzuki Y, Fukui Y, Kawashima M, Takada M, Ibi Y, Haga H, Morita S, Toi M, Kawaoka S, Kawaguchi K
EBioMedicine. 2024 Aug 21;107:105271. doi: 10.1016/j.ebiom.2024.105271

Transport and spatio-temporal conversion of sugar facilitate the formation of spatial gradients of starch in wheat caryopses
Zhong Y, Chen Y, Pan M, Li X, Hebelstrup K, Cai J, Zhou Q, Dai T, Cao W, Jiang D
Commun Biol. 2024 Aug 2;7(1):928. doi: 10.1038/s42003-024-06625-4.

Proteomics, Lipidomics, Metabolomics

Dose optimization of TBI-223 for enhanced therapeutic benefit compared to linezolid in antituberculosis regimen
Strydom N, Ernest JP, Imperial M, Solans BP, Wang Q, Tasneen R, Tyagi S, Soni H, Garcia A, Bigelow K, Gengenbacher M, Zimmerman M, Xie M, Sarathy JP, Yang TJ, Dartois V, Nuermberger EL, Savic RM
Nat Commun. 2024 Aug 25;15(1):7311. doi: 10.1038/s41467-024-50781-4.

Proteomic analysis of laser captured tubular tissues reveals complement activation and mitochondrial dysfunction in autoimmune related kidney diseases
Xiao M, Chi X, Zhu X, Xu Z, Zou Y, Peng Y, Luan S, Dong J, Dai Y, Yin L
Sci Rep. 2024 Aug 20;14(1):19311. doi: 10.1038/s41598-024-70209-9.

Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues
Watanabe K, Takayama S, Yamada T, Hashimoto M, Tadano J, Nakagawa T, Watanabe T, Fukusaki E, Miyawaki I, Shimma S
Anal Bioanal Chem. 2024 Aug 10. doi: 10.1007/s00216-024-05477-5.

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8
Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Fluorescence Applications

Deciphering functional tumor–immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann M
bioRxiv preprint, May 23, 2024

The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma
Hu H, Liu G, Li Y
Plant Methods. 2024 May 17;20(1):72. doi: 10.1186/s13007-024-01184-5.

Deep Visual Proteomics advances human colon organoid models by revealing a switch to an in vivo-like phenotype upon xenotransplantation
Post F, Hausmann A, Kabatnik S, Steigerwald S, Brand A, Clement DL, Skov J, Boyce TL, Sato T, Steenholdt C, Mund A, Nielsen OH, Jensen KB, Mann M
bioRxiv preprint, doi: doi.org/10.1101/2024.05.13.593888; May 14, 2024

Cancer Research

Intra-patient spatial comparison of non-metastatic and metastatic lymph nodes reveals a reduction in CD169+ macrophages within metastatic breast cancers
Maeshima Y, Kataoka TR, Vandenbon A, Hirata M, Takeuchi Y, Suzuki Y, Fukui Y, Kawashima M, Takada M, Ibi Y, Haga H, Morita S, Toi M, Kawaoka S, Kawaguchi K
EBioMedicine. 2024 Aug 21;107:105271. doi: 10.1016/j.ebiom.2024.105271

Differences in BRAF V600E mutation between the epithelium and mesenchyme in classic ameloblastoma
Chen Z, Hong Y, Zhao Z, Wu N, Ma X, Chen L, Zhang R
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 21 Aug 2024

Spatial multiomics reveals a subpopulation of fibroblasts associated with cancer stemness in human hepatocellular carcinoma
Jing SY, Liu D, Feng N, Dong H, Wang HQ, Yan X, Chen XF, Qu MC, Lin P, Yi B, Feng F, Chen L, Wang HY, Li H, He YF
Genome Med. 2024 Aug 13;16(1):98. doi: 10.1186/s13073-024-01367-8.

Neurosciences

rTMS mechanisms for posttraumatic stress disorder treatment in a mouse model
Claverie D, Cressant A, Thomasson J, Castellarin C, Grandperret V, Barbier L, Troubat R, Canini F, Belzung C, El-Hage W
Journal of Psychiatric Research, 28 Aug 2024, S0022-3956(24)00506-5

Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues
Watanabe K, Takayama S, Yamada T, Hashimoto M, Tadano J, Nakagawa T, Watanabe T, Fukusaki E, Miyawaki I, Shimma S
Anal Bioanal Chem. 2024 Aug 10. doi: 10.1007/s00216-024-05477-5.

Analysis of microisolated frontal cortex excitatory layer III and V pyramidal neurons reveals a neurodegenerative phenotype in individuals with Down syndrome
Alldred MJ, Pidikiti H, Ibrahim KW, Lee SH, Heguy A, Hoffman GE, Roussos P, Wisniewski T, Wegiel J, Stutzmann GE, Mufson EJ, Ginsberg SD
Acta Neuropathol. 2024 Aug 6;148(1):16. doi: 10.1007/s00401-024-02768-0.

Clinical/Pathology/Immunology Research Applications

A reliable clinical test for detection of membranous nephropathyantigens using laser microdissection and mass spectrometry
Vrana JA, Theis JD, Wegwerth PJ, Dasari S, Madden B, Nasr SH, Fidler M,McPhail ED, Fervenza FC, Sethi S
Kidney International (2024), doi: doi.org/10.1016/j.kint.2024.07.031

Dose optimization of TBI-223 for enhanced therapeutic benefit compared to linezolid in antituberculosis regimen
Strydom N, Ernest JP, Imperial M, Solans BP, Wang Q, Tasneen R, Tyagi S, Soni H, Garcia A, Bigelow K, Gengenbacher M, Zimmerman M, Xie M, Sarathy JP, Yang TJ, Dartois V, Nuermberger EL, Savic RM
Nat Commun. 2024 Aug 25;15(1):7311. doi: 10.1038/s41467-024-50781-4.

Proteomic analysis of laser captured tubular tissues reveals complement activation and mitochondrial dysfunction in autoimmune related kidney diseases
Xiao M, Chi X, Zhu X, Xu Z, Zou Y, Peng Y, Luan S, Dong J, Dai Y, Yin L
Sci Rep. 2024 Aug 20;14(1):19311. doi: 10.1038/s41598-024-70209-9.

Developmental Research, Epigenetics  & Aging

Collagen-Heparin-FGF2-VEGF Scaffolds Induce a Regenerative Gene Expression Profile in a Fetal Sheep Wound Model
Gansevoort M, Oostendorp C, Bouwman LF, Tiemessen DM, Geutjes PJ, Feitz WFJ, van Kuppevelt TH, Daamen WF
Tissue Eng Regen Med. 2024 Aug 31. doi: 10.1007/s13770-024-00667-9.

Molecular basis of urostyle development in frogs: genes and gene regulation underlying an evolutionary novelty
Senevirathne G, Shubin NH
Open Biol. 2024 Aug;14(8):240111. doi: 10.1098/rsob.240111.

Transcriptional programs of Pitx2 and Tfap2a/Tfap2b controlling lineage specification of mandibular epithelium during tooth initiation
Shao F, Phan AV, Yu W, Guo Y, Thompson J, Coppinger C, Venugopalan SR, Amendt BA, Van Otterloo E, Cao H
PLoS Genet. 2024 Jul 25;20(7):e1011364. doi: 10.1371/journal.pgen.1011364.

Plant Research

Transport and spatio-temporal conversion of sugar facilitate the formation of spatial gradients of starch in wheat caryopse
Zhong Y, Chen Y, Pan M, Li X, Hebelstrup K, Cai J, Zhou Q, Dai T, Cao W, Jiang D
Commun Biol. 2024 Aug 2;7(1):928. doi: 10.1038/s42003-024-06625-4.

Developing a simple and rapid method for cell-specific transcriptome analysis through laser microdissection: insights from citrus rind with broader implications
Mei Z, Zhu K, Yen D, Jia H, Luo W, Ye J, Deng X
Plant Methods. 2024 Jul 27;20(1):113. doi: 10.1186/s13007-024-01242-y.

Soybean symbiotic-nodule zonation and cell differentiation are defined by NIN2 signaling and GH3-dependent auxin homeostasis
Tu T, Gao Z, Li L, Chen J, Ye K, Xu T, Mai S, Han Q, Chen C, Wu S, Dong Y, Chen J, Huang L, Guan Y, Xie F, Chen X
Dev Cell. 2024 Jul 17:S1534-5807(24)00424-6. doi: 10.1016/j.devcel.2024.07.001.

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Miscellaneous

Cytokinetic abscission in Toxoplasma gondii is governed by protein phosphatase 2A and the daughter cell scaffold complex
Marq JB, Gosetto M, Altenried A, Vadas O, Maco B, Dos Santos Pacheco N, Tosetti N, Soldati-Favre D, Lentini G
EMBO J. 2024 Jul 15. doi: 10.1038/s44318-024-00171-9.

Oversampling for Enhanced Spatial Resolution of Zebrafish by Top-Hat IR-MALDESI-MSI
Sohn AL, Bowman AP, Barnes MM, Kullman SW, Muddiman DC
J Am Soc Mass Spectrom. 2024 Jul 10. doi: 10.1021/jasms.4c00219.

Persistent activity of aerobic methane-oxidizing bacteria in anoxic lake waters due to metabolic versatility.
Schorn S, Graf JS, Littmann S, Hach PF, Lavik G, Speth DR, Schubert CJ, Kuypers MMM, Milucka J
Nat Commun. 2024 Jun 21;15(1):5293. doi: 10.1038/s41467-024-49602-5.

Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23

Live Cell Culture

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416

An efficient semi–in vivo zygotic embryogenesis system in Arabidopsis
Li J, Li SS, Liu Y, Tang WY, Liu XF, Meng CF, Zhang LY, Sun MX
In Vitro Cell.Dev.Biol.-Plant, 27. Dec 2022