Leica LMD6 & LMD7 Microdissection laser

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

Method of the Year 2024: spatial proteomics. 
Nat Methods 21, 2195–2196 (2024). 
https://doi.org/10.1038/s41592-024-02565-3

A new understanding of tissue biology from MS-based proteomics at single-cell resolution
Nordmann TM, Mund A, Mann M
Nat Methods 21, 2220–2222 (2024). 

Leveraging deep single-soma RNA sequencing to explore the neural basis of human somatosensation
Yu H, Nagi SS, Usoskin D, Hu Y, Kupari J, Bouchatta O, Yan H, Cranfill SL, Gautam M, Su Y, Lu Y, Wymer J, Glanz M, Albrecht P, Song H, Ming GL, Prouty S, Seykora J, Wu H, Ma M, Marshall A, Rice FL, Li M, Olausson H, Ernfors P, Luo W 
Nat Neurosci. 2024 Nov 4. doi: 10.1038/s41593-024-01794-1.

The proteomic landscape of proteotoxic stress in a fibrogenic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S. Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Kaserman J, Wilson A, Fabián O, Franková S, Krag AA, Strnad P, and Mann M
bioRxiv, 3rd Nov 2024

DNA Research

Exosome-Mediated Transfer of X-Motif-Tagged Anti-MiR-33a-5p Antagomirs to the Medial Cells of Transduced Rabbit Carotid Arteries
Saenz-Pipaon G, Wacker VK, Bi L, Stamatikos A, Dichek DA
Biology 2024, 13(12), 965; 24. Nov 2024

High mitochondrial DNA levels accelerate lung adenocarcinoma progression
Mennuni M, Wilkie SE, Michon P, Alsina D, Filograna R, Lindberg M, Sanin DE, Rosenberger F, Schaaf A, Larsson E, Pearce EL, Larsson NG
Sci Adv. 2024 Nov;10(44):eadp3481. doi: 10.1126/sciadv.adp3481.

Immunotherapy response induces divergent tertiary lymphoid structure morphologies in hepatocellular carcinoma
Shu DH, Ho WJ, Kagohara LT, Girgis A, Shin SM, Danilova L, Lee JW, Sidiropoulos DN, Mitchell S, Munjal K, Howe K, Bendinelli KJ, Kartalia E, Qi H, Mo G, Montagne J, Leatherman JM, Lopez-Vidal TY, Zhu Q, Huff AL, Yuan X, Hernandez A, Coyne EM, Zaidi N, Zabransky DJ, Engle LL, Ogurtsova A, Baretti M, Laheru D, Durham JN, Wang H, Sunshine JC, Johnston RJ, Deutsch JS, Taube JM, Anders RA, Jaffee EM, Fertig EJ, Yarchoan M
Nat Immunol. 2024 Oct 25. doi: 10.1038/s41590-024-01992-w.

RNA Research

Methods for Selective Gene Expression Profiling in Single Tertiary Lymphoid Structure Using Laser Capture Microdissection
Gutierrez-Chavez, C., Knockaert, S., Dieu-Nosjean, MC., Goc, J. 
Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 2864. Humana, New York, NY (2025). 

Laser Capture Microdissection of Tertiary Lymphoid Structures from Formalin-Fixed Paraffin-Embedded Sections of Canine Cutaneous and Subcutaneous Sarcomas for NanoString Direct RNA Counting
Olver, C.S.
Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 2864. Humana, New York, NY (2025). 

Spatiotemporal functions of leukemia inhibitory factor in embryo attachment and implantation chamber formation
Aikawa S, Hiraoka T, Matsuo M, Fukui Y, Fujita H, Saito-Fujita T, Shimizu-Hirota R, Takeda N, Hiratsuka D, He X, Ishizawa C, Iida R, Akaeda S, Harada M, Wada-Hiraike O, Ikawa M, Osuga Y, Hirota Y
Cell Death Discov. 2024 Nov 25;10(1):481. doi: 10.1038/s41420-024-02228-4.

Proteomics, Lipidomics, Metabolomics

A new understanding of tissue biology from MS-based proteomics at single-cell resolution
Nordmann TM, Mund A, Mann M
Nat Methods 21, 2220–2222 (2024). 

Accurate diagnosis of early-stage lung cancer by targeting lipid droplets with a two-photon near-infrared fluorescence probe
Wang D, Wu P, Yang Z, Yu C, Li Q, Zhang R, Yang Y, Liu Y, Zhang W, Zhou X, Zhu D, Zhan Z, Li W
Sensors and Actuators B: Chemical, Volume 426, 2025, 137023, ISSN 0925-4005, doi: 10.1016/j.snb.2024.137023

Persistence of spike protein at the skull-meninges-brain axis may contribute to the neurological sequelae of COVID-19
Rong Z, Mai H, Ebert G, Kapoor S, Puelles VG, Czogalla J, Hu S, Su J, Prtvar D, Singh I, Schädler J, Delbridge C, Steinke H, Frenzel H, Schmidt K, Braun C, Bruch G, Ruf V, Ali M, Sühs KW, Nemati M, Hopfner F, Ulukaya S, Jeridi D, Mistretta D, Caliskan ÖS, Wettengel JM, Cherif F, Kolabas ZI, Molbay M, Horvath I, Zhao S, Krahmer N, Yildirim AÖ, Ussar S, Herms J, Huber TB, Tahirovic S, Schwarzmaier SM, Plesnila N, Höglinger G, Ondruschka B, Bechmann I, Protzer U, Elsner M, Bhatia HS, Hellal F, Ertürk A
Cell Host Microbe. 2024 Nov 26:S1931-3128(24)00438-4. doi: 10.1016/j.chom.2024.11.007.

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Method of the Year 2024: spatial proteomics. 
Nat Methods 21, 2195–2196 (2024). 
https://doi.org/10.1038/s41592-024-02565-3

Highlights 2024: scDVP applied for human health improvement
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0

Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Fluorescence Applications

Publisher Correction: Novel laser model of optic nerve transection provides valuable insights about the dynamics of optic nerve regeneration
Moulin C, Dvoriantchikova G, Bineshfar N, Swingle B, Martinez G, Groso D, Zhang M, Ivanov D, Pelaez D
Sci Rep. 2024 Nov 29;14(1):29691. doi: 10.1038/s41598-024-80215-6.

Spatial proteomics of single cells and organelles on tissue slides using filter-aided expansion proteomics
Dong Z, Jiang W, Wu C, Chen T, Chen J, Ding X, Zheng S, Piatkevich KD, Zhu Y, Guo T
Nat Commun. 2024 Oct 30;15(1):9378.

Spatial proteomics identifies JAKi as treatment for a lethal skin disease
Nordmann TM, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler PC, Sinha A, Metousis A, Rosenberger FA, Zwiebel M, Satoh TK, Anzengruber F, Strauss MT, Tanzer MC, Saito Y, Gong T, Thielert M, Kimura H, Silke N, Rodriguez EH, Restivo G, Nguyen HH, Gross A, Feldmeyer L, Joerg L, Levesque MP, Murray PJ, Ingen-Housz-Oro S, Mund A, Abe R, Silke J, Ji C, French LE, Mann M
Nature. 2024 Oct 16. doi: 10.1038/s41586-024-08061-0.

Cancer Research

Accurate diagnosis of early-stage lung cancer by targeting lipid droplets with a two-photon near-infrared fluorescence probe
Wang D, Wu P, Yang Z, Yu C, Li Q, Zhang R, Yang Y, Liu Y, Zhang W, Zhou X, Zhu D, Zhan Z, Li W
Sensors and Actuators B: Chemical, Volume 426, 2025, 137023, ISSN 0925-4005, doi: 10.1016/j.snb.2024.137023

High mitochondrial DNA levels accelerate lung adenocarcinoma progression
Mennuni M, Wilkie SE, Michon P, Alsina D, Filograna R, Lindberg M, Sanin DE, Rosenberger F, Schaaf A, Larsson E, Pearce EL, Larsson NG
Sci Adv. 2024 Nov;10(44):eadp3481. doi: 10.1126/sciadv.adp3481.

Protumoral lipid droplet-loaded macrophages are enriched in human glioblastoma and can be therapeutically targeted
Governa V, de Oliveira KG, Bång-Rudenstam A, Offer S, Cerezo-Magaña M, Li J, Beyer S, Johansson MC, Månsson AS, Edvardsson C, Durmo F, Gustafsson E, Boukredine A, Jeannot P, Schmidt K, Gezelius E, Menard JA, Garza R, Jakobsson J, de Neergaard T, Sundgren PC, Tiihonen AM, Haapasalo H, Rautajoki KJ, Nordenfelt P, Darabi A, Forsberg-Nilsson K, Pietras A, Talbot H, Bengzon J, Belting M
Sci Transl Med. 2024 Oct 30;16(771):eadk1168. doi: 10.1126/scitranslmed.adk1168.

Neurosciences

Persistence of spike protein at the skull-meninges-brain axis may contribute to the neurological sequelae of COVID-19
Rong Z, Mai H, Ebert G, Kapoor S, Puelles VG, Czogalla J, Hu S, Su J, Prtvar D, Singh I, Schädler J, Delbridge C, Steinke H, Frenzel H, Schmidt K, Braun C, Bruch G, Ruf V, Ali M, Sühs KW, Nemati M, Hopfner F, Ulukaya S, Jeridi D, Mistretta D, Caliskan ÖS, Wettengel JM, Cherif F, Kolabas ZI, Molbay M, Horvath I, Zhao S, Krahmer N, Yildirim AÖ, Ussar S, Herms J, Huber TB, Tahirovic S, Schwarzmaier SM, Plesnila N, Höglinger G, Ondruschka B, Bechmann I, Protzer U, Elsner M, Bhatia HS, Hellal F, Ertürk A
Cell Host Microbe. 2024 Nov 26:S1931-3128(24)00438-4. doi: 10.1016/j.chom.2024.11.007.

Deep RNA sequencing of human dorsal root ganglion neurons reveals somatosensory mechanisms
Yu H, Luo W
Nat Neurosci (6 Nov 2024). 

Leveraging deep single-soma RNA sequencing to explore the neural basis of human somatosensation
Yu H, Nagi SS, Usoskin D, Hu Y, Kupari J, Bouchatta O, Yan H, Cranfill SL, Gautam M, Su Y, Lu Y, Wymer J, Glanz M, Albrecht P, Song H, Ming GL, Prouty S, Seykora J, Wu H, Ma M, Marshall A, Rice FL, Li M, Olausson H, Ernfors P, Luo W
Nat Neurosci. 2024 Nov 4. doi: 10.1038/s41593-024-01794-1.

Clinical/Pathology/Immunology Research Applications

Microvascular endothelial activation in mouse sepsis: Focus on inflammatory activation and organ-specific responses
Wang Z
Thesis fully internal (DIV), University of Groningen

Persistence of spike protein at the skull-meninges-brain axis may contribute to the neurological sequelae of COVID-19
Rong Z, Mai H, Ebert G, Kapoor S, Puelles VG, Czogalla J, Hu S, Su J, Prtvar D, Singh I, Schädler J, Delbridge C, Steinke H, Frenzel H, Schmidt K, Braun C, Bruch G, Ruf V, Ali M, Sühs KW, Nemati M, Hopfner F, Ulukaya S, Jeridi D, Mistretta D, Caliskan ÖS, Wettengel JM, Cherif F, Kolabas ZI, Molbay M, Horvath I, Zhao S, Krahmer N, Yildirim AÖ, Ussar S, Herms J, Huber TB, Tahirovic S, Schwarzmaier SM, Plesnila N, Höglinger G, Ondruschka B, Bechmann I, Protzer U, Elsner M, Bhatia HS, Hellal F, Ertürk A
Cell Host Microbe. 2024 Nov 26:S1931-3128(24)00438-4. doi: 10.1016/j.chom.2024.11.007.

Exosome-Mediated Transfer of X-Motif-Tagged Anti-MiR-33a-5p Antagomirs to the Medial Cells of Transduced Rabbit Carotid Arteries
Saenz-Pipaon G, Wacker VK, Bi L, Stamatikos A, Dichek DA
Biology 2024, 13(12), 965; 24. Nov 2024

Developmental Research, Epigenetics  & Aging

Spatiotemporal functions of leukemia inhibitory factor in embryo attachment and implantation chamber formation
Aikawa S, Hiraoka T, Matsuo M, Fukui Y, Fujita H, Saito-Fujita T, Shimizu-Hirota R, Takeda N, Hiratsuka D, He X, Ishizawa C, Iida R, Akaeda S, Harada M, Wada-Hiraike O, Ikawa M, Osuga Y, Hirota Y
Cell Death Discov. 2024 Nov 25;10(1):481. doi: 10.1038/s41420-024-02228-4.

Slc26a2-mediated sulfate metabolism is significant for the tooth development
Yoshida Y, Inubushi T, Yokoyama M, Nag P, Sasaki JI, Oka A, Murotani T, Kani R, Shiraishi Y, Kurosaka H, Takahata Y, Nishimura R, Imazato S, Papagerakis P, Yamashiro T
Dis Model Mech dmm.052107. 7 Nov 2024

Cell-cell interaction determines cell fate of mesoderm-derived cell in tongue development through Hh signaling
Kawasaki M, Kawasaki K, Sari FT, Kudo T, Nihara J, Kitamura M, Nagai T, Utama V, Ishida Y, Meguro F, Kesuma A, Fujita A, Nishimura T, Kogure Y, Maruyama S, Tanuma JI, Kakihara Y, Maeda T, Ghafoor S, Khonsari RH, Corre P, Sharpe PT, Cobourne M, Franco B, Ohazama A
Elife. 2024 Oct 11;13:e85042. doi: 10.7554/eLife.85042.

Plant Research

RNA Extraction from Rice Immature Embryo Using Laser Capture Microdissection
Tezuka T, Nosaka-Takahashi M, Yoshikawa T, Sato Y
Methods in Molecular Biology, vol 2869. (2025) in: Kawakatsu, T. (eds) Rice. 

Transport and spatio-temporal conversion of sugar facilitate the formation of spatial gradients of starch in wheat caryopse
Zhong Y, Chen Y, Pan M, Li X, Hebelstrup K, Cai J, Zhou Q, Dai T, Cao W, Jiang D
Commun Biol. 2024 Aug 2;7(1):928. doi: 10.1038/s42003-024-06625-4.

Developing a simple and rapid method for cell-specific transcriptome analysis through laser microdissection: insights from citrus rind with broader implications
Mei Z, Zhu K, Yen D, Jia H, Luo W, Ye J, Deng X
Plant Methods. 2024 Jul 27;20(1):113. doi: 10.1186/s13007-024-01242-y.

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Miscellaneous

Methods for Selective Gene Expression Profiling in Single Tertiary Lymphoid Structure Using Laser Capture Microdissection
Gutierrez-Chavez, C., Knockaert, S., Dieu-Nosjean, MC., Goc, J.
Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 2864. Humana, New York, NY (2025). 

Laser Capture Microdissection of Tertiary Lymphoid Structures from Formalin-Fixed Paraffin-Embedded Sections of Canine Cutaneous and Subcutaneous Sarcomas for NanoString Direct RNA Counting
Olver, C.S.
Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 2864. Humana, New York, NY (2025). 

RNA Extraction from Rice Immature Embryo Using Laser Capture Microdissection
Tezuka T, Nosaka-Takahashi M, Yoshikawa T, Sato
Methods in Molecular Biology, vol 2869. (2025) in: Kawakatsu, T. (eds) Rice. 

PET imaging of GABAA receptors in pancreatic islets by [11C]flumazenil
Maloum-Rami F, Cheung P, Antoni G, Jin Z, Eriksson O, Espes D
EJNMMI Res. 2024 Dec 2;14(1):122. doi: 10.1186/s13550-024-01176-5.

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Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23

Live Cell Culture

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416