Contactez-nous

Leica LMD6 & LMD7 Microdissection laser

La dissection parfaite

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Now there is single-cell Deep Visual Proteomics (scDVP), a technology that integrates high-content imaging, laser microdissection, and multiplexed mass spectrometry (MS) for tissue characterization at the single cell level.

Download the complete list of LMD publications in "Downloads"

Single cell applications

Laser capture microdissection for biomedical research: towards high-throughput, multi-omics, and single-cell resolution
Guo W, Hu Y, Qian J, Zhu L, Cheng J, Liao J, Fan X
J Genet Genomics. 2023 Aug 4:S1673-8527(23)00163-7. doi: 10.1016/j.jgg.2023.07.011.

Multiplex-GAM: genome-wide identification of chromatin contacts yields insights overlooked by Hi-C
Beagrie RA, Thieme CJ, Annunziatella C, Baugher C, Zhang Y, Schueler M, Kukalev A, Kempfer R, Chiariello AM, Bianco S, Li Y, Davis T, Scialdone A, Welch LR, Nicodemi M, Pombo A
Nat Methods. 2023 Jun 19. doi: 10.1038/s41592-023-01903-1.

Single-Cell Analysis in the OMICS Era: Technologies and Applications in Cancer
Massimino, M.; Martorana, F.; Stella, S.; Vitale, S.R.; Tomarchio, C.; Manzella, L.; Vigneri, P.
Genes 2023, 14, 1330.

DNA Research

Strategic approach to heterogeneity analysis of cutaneous adnexal carcinomas using computational pathology and genomics
Nishimura T, Eijitsu R, Inoue S, Kawazu M, Ueno T, Namikawa K, Takahashi A, OgataD, Yoshida A, Yamazaki N, Mano H, Yatabe Y, Mori T
JID Innovations, Skin Science from Molecules to Population Health, 05 Sep 2023

Molecular biological analysis revealed a case of synchronous endometrial and ovarian cancer with different histological grade as metastatic ovarian cancer from endometrial cancer: Case report and review of literature
Yumisashi R, Saito R, Togami S, Kobayashi Y, Kitazono I, Tanimoto A, Kobayashi H
J Obstet Gynaecol Res. 2023 Aug 21. doi: 10.1111/jog.15773.

Evolutionary histories of breast cancer and related clones
Nishimura T, Kakiuchi N, Yoshida K, Sakurai T, Kataoka TR, Kondoh E, Chigusa Y, Kawai M, Sawada M, Inoue T, Takeuchi Y, Maeda H, Baba S, Shiozawa Y, Saiki R, Nakagawa MM, Nannya Y, Ochi Y, Hirano T, Nakagawa T, Inagaki-Kawata Y, Aoki K, Hirata M, Nanki K, Matano M, Saito M, Suzuki E, Takada M, Kawashima M, Kawaguchi K, Chiba K, Shiraishi Y, Takita J, Miyano S, Mandai M, Sato T, Takeuchi K, Haga H, Toi M, Ogawa S
Nature. 2023 Jul 26. doi: 10.1038/s41586-023-06333-9.

RNA Research

Mature human induced pluripotent stem cell-derived cardiomyocytes promote angiogenesis through alpha-B crystallin
Tanaka Y, Kadota S, Zhao J, Kobayashi H, Okano S, Izumi M, Honda Y, Ichimura H, Shiba N, Uemura T, Wada Y, Chuma S, Nakada T, Tohyama S, Fukuda K, Yamada M, Seto T, Kuwahara K, Shiba Y
Stem Cell Res Ther. 2023 Sep 7;14(1):240. doi: 10.1186/s13287-023-03468-4.

Peptide YY: A Paneth cell antimicrobial peptide that maintains Candida gut commensalism
Pierre JF, Peters BM, La Torre D, Sidebottom AM, Tao Y, Zhu X, Cham CM, Wang L, Kambal A, Harris KG, Silva JF, Zaborina O, Alverdy JC, Herzog H, Witchley J, Noble SM, Leone VA, Chang EB
Science. 2023 Aug 4;381(6657):502-508. doi: 10.1126/science.abq3178.

Unique miRNome and Transcriptome Profiles Underlie Microvascular Heterogeneity in Mouse Kidney
Luxen M, Zwiers PJ, Meester F, Jongman RM, Kuiper T, Moser J, Pultar M, Skalicky S, Diendorfer AB, Hackl M, van Meurs M, Molema G
Am J Physiol Renal Physiol. 2023 Jul 6. doi: 10.1152/ajprenal.00005.2023.

Proteomics, Lipidomics, Metabolomics

Defensive furofuran lignans localized to the oil cells of Neocinnamomum delavayi and their metabolism by a specialist insect
Yang MJ, Luo SH, Liu Y, Li SH
Phytochemistry. 2023 Sep 8:113852. doi: 10.1016/j.phytochem.2023.113852.

Mass Spectrometry-Based Analysis of Histone Posttranslational Modifications from Laser Microdissected Samples
Noberini R, Bonaldi T
Methods Mol Biol. 2023;2718:271-283. doi: 10.1007/978-1-0716-3457-8_15.

Laser microdissection, proteomics, and multiplex immunohistochemistry: a bumpy ride into the study of paraffin-embedded fetal and pediatric lung tissues
Cardoso Dos Santos LM, Avila Y, Schvartz D, Rougemont AL, Bochaton-Piallat ML, Ruchonnet-Metrailler I
Front Med (Lausanne). 2023 Aug 29;10:1191205. doi: 10.3389/fmed.2023.1191205.

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
Mund A, Coscia F, Kriston A, Hollandi R, Kovacs F, Brunner A-D, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
Nat Biotechnol (2022). doi.org/10.1038/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8
Now even on single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1101/2022.12.03.518957

Fluorescence Applications

Root Primordium Defective 1 Encodes an Essential PORR Protein Required for the Splicing of Mitochondria Encoded Group II Introns and for Respiratory Complex I Biogenesis
Edris R, Sultan LD, Best C, Mizrahi R, Weinstein O, Chen S, Kamennaya NA, Keren N, Zer H, Zhu H, Ostersetzer-Biran O
Plant Cell Physiol. 2023 Sep 13:pcad101. doi: 10.1093/pcp/pcad101.

Organized B cell sites in cartilaginous fishes reveal the evolutionary foundation of germinal centers
Matz H, Taylor RS, Redmond AK, Hill TM, Ruiz Daniels R, Beltran M, Henderson NC, Macqueen DJ, Dooley H
Cell Rep. 2023 Jun 20;42(7):112664. doi: 10.1016/j.celrep.2023.112664.

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416

Cancer Research

Neurosciences

Transcriptomic Profiling of Early Synucleinopathy in Rats Induced with Preformed Fibrils
Patterson J, Kochmanski J, Stoll A, Kubik M, Kemp C, Duffy M, Thompson K, Howe J, Cole-Strauss A, Kuhn N, Miller K, Nelson S, Onyekpe C, Beck J, Counts S, Bernstein A, Steece-Collier K, Luk K, Sortwell C
Research Square, 18 Aug 2023

Localized Proteomic Differences in Choroid Plexus of Alzheimer's Disease and Epilepsy Patients
Leitner DF, Kanshin E, Faustin A, Thierry M, Friedman D, Devore S, Ueberheide B, Devinsky O , Wisniewski T
Front. Neurol., Sec. Dementia and Neurodegenerative Diseases, Volume 14 - 2023 | doi: 10.3389/fneur.2023.1221775

Basal forebrain cholinergic neurons are vulnerable in a mouse model of Down syndrome and their molecular fingerprint is rescued by maternal choline supplementation
Alldred MJ, Pidikiti H, Heguy A, Roussos P, Ginsberg SD
FASEB J. 2023 Jun;37(6):e22944. doi: 10.1096/fj.202202111RR.

Clinical/Pathology/Immunology Research Applications

Toll-like receptor 9-positive plasmacytoid dendritic cells promote Th17 immune responses in oral lichen planus stimulated by epithelium-derived cathepsin K
Miyahara Y, Hu C, Moriyama M, Kaneko N, Haque RASM, Chinju A, Kai K, Sakamoto M, Kakizoe-Ishiguro N, Yamauchi M, Ogata K, Kiyoshima T, Kawano S, Nakamura S
Research Square, 21 Aug 2023

Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection
Cai X, Zhang H, Zhang J, Li T
J. Vis. Exp. (198), e65890, doi:10.3791/65890 (11 Aug 2023).

Developmental Research, Epigenetics & Aging

Androgenetic/biparental mosaicism in a diploid mole-like conceptus: report of a case with triple paternal contribution
Donzel M, Gaillot-Durand L, Joubert M, Aziza J, Beneteau C, Mauduit C, Ploteau S, Hajri T, Bolze PA, Massardier J, Devouassoux-Shisheboran M, Sunde L, Allias F
Virchows Arch. 2023 Sep 11. doi: 10.1007/s00428-023-03638-y.

Plant Research

Plant and fungal gene expression coupled with stable isotope labelling provide novel information on sulfur uptake and metabolism in orchid mycorrhizal protocorms
De Rose S, Kuga Y, Sillo F, Fochi V, Sakamoto N, Calevo J, Perotto S, Balestrini R
Plant J. 2023 Jul 8. doi: 10.1111/tpj.16381.

Forensics

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
Sun M, Zheng J, Wnag B and Yao J:
BioMed Research International, Volume 2021 | Article ID 7269237

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Further information on Leica LMD impact at forensics:
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Development of a sample preparation method for micro-proteomics analysis of the formaldehyde-fixed paraffin-embedded liver tissue samples
Wang YE, Zeng WL, Cao ST, Zou JP, Liu CT, Shi JM, Li J, Qiu F, Wang Y
Talanta. 2023 Aug 23;266(Pt 2):125106. doi: 10.1016/j.talanta.2023.125106

Gold-FISH enables targeted NanoSIMS analysis of plant-associated bacteria
Schmidt H, Gorka S, Seki D, Schintlmeister A, and Woebken D
New Phytol. 2023 Jun 28. doi: 10.1111/nph.19112.

Mechanical waves identify the amputation position during wound healing in the amputated zebrafish tailfin
De Leon MP, Wen FL, Paylaga GJ, Wang YT, Roan HY, Wang CH, Hsiao CD, Lin KH & Chen CH
Nat. Phys. (19 Jun 2023).

Live Cell Culture

An efficient semi–in vivo zygotic embryogenesis system in Arabidopsis
Li J, Li SS, Liu Y, Tang WY, Liu XF, Meng CF, Zhang LY, Sun MX
In Vitro Cell.Dev.Biol.-Plant, 27. Dec 2022

Direct Reprogramming of Mouse Subchondral Bone Osteoblasts into Chondrocyte-Like Cells
Li M, Zhang L, Li J, and Zhu Q
Biomedicines 2022, 10, 2582.

Heterotypic cell-in-cell structures between cancer and NK cells are associated with enhanced anticancer drug resistance
Choe YJ, Min JY, Lee H, Lee SY, Kwon J, Kim HJ, Lee J, Kim HM, Park HS, Cho MY, Hyun JY, Kim HM, Chung YH, Ha SK, Jeong HG, Choi I, Kim TD, Hong KS, and Han EH
iScience 25, 105017 September 16, 2022