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Leica TCS SP5 II Confocal Microscopes Leica Leica Microsystems

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Leica TCS SP5 II

Multi-color imaging in whole mount specimen

Platyneris spec.

blue: nuclei, DAPI; green: Actin/muscles, TRITC; red: Tubulin, Alexa 633

Courtesy of: Dr. Evgeny Tsitrin, Institute of Developmental Biology RAS, Moscow, Russia

 

Imaging and manipulation with new specific optics

The optics of the Leica TCS SP5 II is optimized for imaging and manipulation. The beam expander allows for switching between outstanding precision and high bleach power. This means that there is no impairment of optical properties if bleaching or imaging.

High resolution and high speed confocal imaging

The Leica Tandem Scanner combines two technological solutions in one system: a conventional scanning system - ideal for morphological imaging and three dimensional structure analyses, and a resonant scanning system - the best solution for high speed confocal imaging.
Merging both systems into one single device, the TCS SP5 II is the ideal solution for imaging core facilities and other multi-user environments.

The fastest true confocal

The combined efficiency of the Leica SP® detection system and the Leica AOBS® guarantees best signal to noise – which means clear and crisp images and least bleaching during image acquisition. Easy operation and a minimum of maintenance save time and money and ensures more results in less time.

Electrophysiology with Leica DM6000 CFS

Small neuron network: rat brain slice, layer 5. Loading of dyes by single cell electroporation.

Loading of dyes by single cell electroporationred: Interneurons Alexa 594; green: Pyramidal Cell Oregon Bapta 1 (calcium sensitive)

Z = 123 µm; two-photon excitation; detection with a 2 channel NDD

Courtesy of Dr. Thomas Nevian, Institute fo Physiology, University of Bern, Switzerland

Deep tissue imaging with multi-photon microscopy

Artery of the mouse: Excitation at 839 nm, 3-channel acquisition: autofluorescence of elastin (blue), Syto13 for nuclei of cells in the vascular wall (green/white), eosin auto-fluorescence (red); zoom 1. Imaging depth 650 µm. Preparation: Common cartoid arteries from mice are carefully dissected, excised, and stored in Hank's balanced salt solution (HBSS, pH 7.4). A single artery is mounted in two glass micropipettes in a homebuilt perfusion chamber and perfused gently with HBSS to remove residual blood.

The distance between the pipettes can be modified to adjust for shortening of the arteries during the isolation process. Afterwards, a transmural pressure of 80 mm Hg is applied to mimic a more physiological situation.

Image acquired with Leica DM6000 CFS

Courtesy of Dr. Marc van Zandvoort, Biophysics, University of Maastricht, Netherlands