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Differential Interference Contrast (DIC) Microscopy
This article demonstrates how differential interference contrast (DIC) can be actually better than brightfield illumination when using microscopy to image unstained biological specimens.
Phase Contrast and Microscopy
This article explains phase contrast, an optical microscopy technique, which reveals fine details of unstained, transparent specimens that are difficult to see with common brightfield illumination.
Immersion Objectives
How an immersion objective, which has a liquid medium between it and the specimen being observed, helps increase the numerical aperture and microscope resolution is explained in this article.
Mechanically Stressed Cells under the Microscope
Traumatic brain injury, skin scarring, or fibrotic heart remodeling are examples for mechanical stress which cells and tissues are exposed to. In this article, the authors introduce a non-invasive,…
Hydraulics in the Automotive and Aerospace Industries
Cleanliness standards relating to lubricants, hydraulic fluids, and oils, e.g., ISO 4406 and DIN 51455 are discussed in this article. Cleanliness plays a central role in the automotive and…
Studying Pulmonary Fibrosis
The results shown in this article demonstrate that fibrotic and non-fibrotic regions of collagen present in mouse lung tissue can be distinguished better with polarized light compared to brightfield.…
Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
The Fundamentals and History of Fluorescence and Quantum Dots
At some point in your research and science career, you will no doubt come across fluorescence microscopy. This ubiquitous technique has transformed the way in which microscopists can image, tag and…
Koehler Illumination: A Brief History and a Practical Set Up in Five Easy Steps
The technique of Koehler Illumination is one of the most important and fundamental techniques in achieving optimum imaging in any given light microscope set-up. Although it should be routinely used as…
Milestones in Incident Light Fluorescence Microscopy
Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…
Video Tutorial: How to Align the Bulb of a Fluorescence Lamp Housing
The traditional light source for fluorescence excitation is a fluorescence lamp housing with mercury burner. A prerequisite for achieving bright and homogeneous excitation is the correct centering and…
Video Tutorial: How to Change the Bulb of a Fluorescence Lamp Housing
When applying fluorescence microscopy in biological applications, a lamp housing with mercury burner is the most common light source. This video tutorial shows how to change the bulb of a traditional…
Fluorescent Proteins - From the Beginnings to the Nobel Prize
Fluorescent proteins are the fundament of recent fluorescence microscopy and its modern applications. Their discovery and consequent development was one of the most exciting innovations for life…
Optical Contrast Methods
Optical contrast methods give the potential to easily examine living and colorless specimens. Different microscopic techniques aim to change phase shifts caused by the interaction of light with the…
Polarization Contrast
Polarization microscopy is routinely applied in material sciences and geology to identify minerals on the basis of characteristic refraction properties and colors. In biology, polarization microscopy…
Fluorescence in Microscopy
Fluorescence microscopy is a special form of light microscopy. It uses the ability of fluorochromes to emit light after being excited with light of a certain wavelength. Proteins of interest can be…
Forensic Detection of Sperm from Sexual Assault Evidence
The impact of modern scientific methods on the analysis of crime scene evidence has dramatically changed many forensic sub-specialties. Arguably one of the most dramatic examples is the impact of…
Multi-Wavelength Epi-Illumination in Fluorescence Microscopy
This article discusses the development of epi-illumination and reflection contrast for fluorescence microscopy concerning life-science applications. Much was done by the Ploem research group…
Campos de aplicación
Microscopía de fluorescencia
La fluorescencia es uno de los fenómenos físicos más utilizados en la microscopía biológica y analítica, principalmente por su alta sensibilidad y alta especificidad. Averigüe cómo los microscopios de…
Virología
¿Su interés en investigación se centra en infecciones víricas y enfermedades? Descubra cómo puede adquirir conocimientos sobre virología con soluciones de generación de imágenes y preparación de…
Técnicas básicas de microscopía
Se utilizan técnicas básicas de microscopía en las que todo el espécimen en la platina del microscopio se expone a una fuente de luz. Todo el espécimen se ilumina con luz blanca desde arriba (en una…
Microscopios DIC
Un microscopio DIC es un microscopio de campo ancho que tiene un filtro de polarización y un prisma Wollaston entre la fuente de luz y la lente del condensador, así como entre la lente del objetivo y…
Microscopios de campo oscuro
El método de contraste de campo oscuro aprovecha la difracción o dispersión de la luz de las estructuras de un espécimen biológico o las características no uniformes de una muestra de material.