Leica LMD6 & LMD7 Microdissezione laser

This curated list features recent scientific publications that have employed Leica’s LMD6 and LMD7 laser microdissection systems in key areas such as cancer research, neuroscience, molecular pathology, forensics, and spatial omics. It is updated regularly to reflect the latest scientific publications utilizing laser microdissection applications.

Explore the full list below to see how Leica LMD systems are enabling scientific success across the globe.

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Single Cell Applications

Deep visual proteomics uncovers nociceptor diversity and pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
Nat Commun. 2026 Apr 11;17(1):3437. doi: 10.1038/s41467-026-71418-8.

Maternal Choline Supplementation in a Mouse Model of Down Syndrome and Alzheimer's Disease Generates Unique Expression Profile Mosaics Within Three Hippocampal Excitatory Neuronal Populations
Alldred MJ, Ibrahim KW, Pidikiti H, Lee SH, Heguy A, Chiosis G, Mufson EJ, Stutzmann GE, Ginsberg SD
FASEB J. 2026 Apr 15;40(7):e71625. doi: 10.1096/fj.202504243R.

Pathogenic Role of SERPINB3-positive Neutrophils in Reinforcing Thrombus Stiffening during Ischemic Stroke
Zang J, Lu A, Yang B, Tan N, Liu Q, Wei L, Liang Y, Zhou S, Tan Z, Xin X, He S, Wu P, Li Y, Xu Z, Su X, Mai H, Xu A, Lu D
Blood. 2026 Mar 3:blood.2025029516. doi: 10.1182/blood.2025029516.

Spatial Genomics (DNA-Reserach)

Clonal biases dictate availability of colonic cancer driver mutations for transformation
Skoufou-Papoutsaki N, Kemp R, Adler S, Marks K, Girard AC, Mehmed S, Lourenço FC, Moutin EB, Ten Hoopen R, Morrissey E, Winton DJ, Tourigny DS
Nat Commun. 2026 Apr 16. doi: 10.1038/s41467-026-71944-5

Polyclonal selection of immune checkpoint mutations in thyroid autoimmunity
Nicola PA, Lawson ARJ, Tidd A, Imbert J, Ishida Y, Wylie LA, Scott PA, Roberts K, Harvey LMR, Lensing SV, Cheng W, Abascal F, Leongamornlert D, Hooks Y, Mayho M, Müller-Sienerth N, Widaa S, Mincarelli L, Illing J, Peci F, Ng BL, Jarman GL, Russell AJC, Mahbubani KTA, Saeb-Parsy K, Paterson AL, Chatterjee K, Rahbari R, Bayraktar OA, Stratton MR, Campbell PJ, Tadross JA, Schoenmakers N, Martincorena I
Nature. 2026 Apr 14. doi: 10.1038/s41586-026-10493-9.

New insights into the feeding behaviour of the Japanese squid Todarodes pacificus paralarvae, and a combined analysis of metagenome and amino acid isotope ratios
Adachi K, Chikaraishi Y, Nomura S, Goto-Inoue N, Zaima N, Kuriya Y, Araki M, Morioka K, Yanagimoto T, Nakaya M, Yamamoto J
PLoS One. 2026 Mar 16;21(3):e0340579. doi: 10.1371/journal.pone.0340579.

Spatial Transcriptomics (RNA Research)

CD44 expression in renal tubules during maladaptive repair is a potential marker for the transition from acute kidney injury to chronic kidney disease in rats
Matsushita K, Akane H, Akagi JI, Morikawa T, Mizuta Y, Ogawa K, Toyoda T
Toxicology. 2026 Apr 20:154473. doi: 10.1016/j.tox.2026.154473.

Laser Capture Microdissection of Paraformaldehyde-Fixed Mouse Liver Tissue for RNA Analysis
Zen WL, Zhang MM, Zeng WJ, Wang Y
JoVE, April 17th, 2026; DOI: 10.3791/69814

Anti-PECAM-1 antibodies: another tool for visualization of Reissner´s fiber and the subcommissural organ in rat central nervous system.
Malčický L, Košuth J, Barčák D, Zrubáková J, Vancová M, Daxnerová Z, Ševc J, Alexovič Matiašová A
Brain Struct Funct. 2026 Apr 4;231(3):50. doi: 10.1007/s00429-026-03088-7.

Proteomics, Lipidomics, Metabolomics

AI-powered Deep Visual Proteomics reveals critical molecular transitions in pancreatic cancer precursors
Min J, Schweizer L, Zonderland G, Chellakkan Selvanesan B, Thomsen JH, Oldenburg L, Bae SW, Kim B, Hernandez SD, Jez G, Bernard V, Swanson BJ, Klute KA, Wang H, Caffrey TC, Grandgenett PM, Hollingsworth MA, Ummat I, Strauss MT, Mund A, Maitra A
Cancer Discov. 2026 Apr 21. doi: 10.1158/2159-8290.CD-25-1119.

Preparation Of Neovascular Tissues from Human Glioma Tissues for Quantitative Proteomics Analysis of Tumor Angiogenesis
Feng X, Fang Z, Zhan X, Guo T, Zhang Y, Yin Z, Zhang Y, Li N, Zhan X
Journal of Visualized Experiments , 2026 (229), DOI: 10.3791/69547

Pan-cancer multi-omic ERBB2-HER2 characterization using next-generation sequencing and quantitative proteomics
Hunt AL, Randall J, Ogata JD, Johnston L, Swain W, Melvin S, Sharma M, Calvert V, Maxwell GL, Bateman NW, Petricoin EF, Conrads TP, Cannon TL
NPJ Precis Oncol. 2026 Mar 5. doi: 10.1038/s41698-026-01351-y.

Deep Visual Proteomics (DVP) related

Evolution in single-cell proteomics drives new frontiers in cancer biology and precision medicine
Liu Y, Du Y, Su R, and Chen 
Cancer Biol Med 2026. doi: 10.20892/j.issn.2095-3941.2025.0464

AI-powered Deep Visual Proteomics reveals critical molecular transitions in pancreatic cancer precursors
Min J, Schweizer L, Zonderland G, Chellakkan Selvanesan B, Thomsen JH, Oldenburg L, Bae SW, Kim B, Hernandez SD, Jez G, Bernard V, Swanson BJ, Klute KA, Wang H, Caffrey TC, Grandgenett PM, Hollingsworth MA, Ummat I, Strauss MT, Mund A, Maitra A
Cancer Discov. 2026 Apr 21. doi: 10.1158/2159-8290.CD-25-1119.

Deep visual proteomics uncovers nociceptor diversity and pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
Nat Commun. 2026 Apr 11;17(1):3437. doi: 10.1038/s41467-026-71418-8.

Single-cell spatial proteomics maps human liver zonation patterns and their vulnerability to disruption in tissue architecture
Weiss CAM, Brown LA, Miranda L, Pellizzoni P, Steigerwald S, Remmert K, Hernandez JM, Borgwardt K, Kleiner DE, Ben-Moshe S, Rosenberger FA, Porat-Shliom N, Mann M
Nat Metab. 2026 Feb 20. doi: 10.1038/s42255-026-01459-2.

Fluorescence Applications

Deep visual proteomics uncovers nociceptor diversity and pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
Nat Commun. 2026 Apr 11;17(1):3437. doi: 10.1038/s41467-026-71418-8.

Pathogenic Role of SERPINB3-positive Neutrophils in Reinforcing Thrombus Stiffening during Ischemic Stroke
Zang J, Lu A, Yang B, Tan N, Liu Q, Wei L, Liang Y, Zhou S, Tan Z, Xin X, He S, Wu P, Li Y, Xu Z, Su X, Mai H, Xu A, Lu D
Blood. 2026 Mar 3:blood.2025029516. doi: 10.1182/blood.2025029516.

Laser  Capture  Microdissection:  Methods  and  Protocols: A  Spatial  Proteomics  Approach  to  Isolate  and  Analyze Amyloid  Proteins  Using  Laser  Capture  Microdissection and  Mass  Spectrometry
Aguilan J, Horton M, Pessin JE, and Sidoli S in Roberta  Noberini  (ed.)
Methods  in  Molecular  Biology,  vol.  3015

Cancer Research

AI-powered Deep Visual Proteomics reveals critical molecular transitions in pancreatic cancer precursors
Min J, Schweizer L, Zonderland G, Chellakkan Selvanesan B, Thomsen JH, Oldenburg L, Bae SW, Kim B, Hernandez SD, Jez G, Bernard V, Swanson BJ, Klute KA, Wang H, Caffrey TC, Grandgenett PM, Hollingsworth MA, Ummat I, Strauss MT, Mund A, Maitra A
Cancer Discov. 2026 Apr 21. doi: 10.1158/2159-8290.CD-25-1119.

Clonal biases dictate availability of colonic cancer driver mutations for transformation
Skoufou-Papoutsaki N, Kemp R, Adler S, Marks K, Girard AC, Mehmed S, Lourenço FC, Moutin EB, Ten Hoopen R, Morrissey E, Winton DJ, Tourigny DS
Nat Commun. 2026 Apr 16. doi: 10.1038/s41467-026-71944-5

Preparation Of Neovascular Tissues from Human Glioma Tissues for Quantitative Proteomics Analysis of Tumor Angiogenesis
Feng X, Fang Z, Zhan X, Guo T, Zhang Y, Yin Z, Zhang Y, Li N, Zhan X
Journal of Visualized Experiments , 2026 (229), DOI: 10.3791/69547

Neurosciences

Deep visual proteomics uncovers nociceptor diversity and pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
Nat Commun. 2026 Apr 11;17(1):3437. doi: 10.1038/s41467-026-71418-8.

Maternal Choline Supplementation in a Mouse Model of Down Syndrome and Alzheimer's Disease Generates Unique Expression Profile Mosaics Within Three Hippocampal Excitatory Neuronal Populations
Alldred MJ, Ibrahim KW, Pidikiti H, Lee SH, Heguy A, Chiosis G, Mufson EJ, Stutzmann GE, Ginsberg SD
FASEB J. 2026 Apr 15;40(7):e71625. doi: 10.1096/fj.202504243R.

Anti-PECAM-1 antibodies: another tool for visualization of Reissner´s fiber and the subcommissural organ in rat central nervous system.
Malčický L, Košuth J, Barčák D, Zrubáková J, Vancová M, Daxnerová Z, Ševc J, Alexovič Matiašová A
Brain Struct Funct. 2026 Apr 4;231(3):50. doi: 10.1007/s00429-026-03088-7.

Clinical/Pathology/Immunology Research Applications

066 – Development of Mass Spectrometry-based Integrative Spatial Multiomics Approach for Metabolic Synovitis Endotyping in Osteoarthritis
Zhu L, Lau CML, Wen C
Osteoarthritis and Cartilage, Volume 34, Supplement, April 2026, Pages S75-S76

CD44 expression in renal tubules during maladaptive repair is a potential marker for the transition from acute kidney injury to chronic kidney disease in rats
Matsushita K, Akane H, Akagi JI, Morikawa T, Mizuta Y, Ogawa K, Toyoda T
Toxicology. 2026 Apr 20:154473. doi: 10.1016/j.tox.2026.154473.

Polyclonal selection of immune checkpoint mutations in thyroid autoimmunity
Nicola PA, Lawson ARJ, Tidd A, Imbert J, Ishida Y, Wylie LA, Scott PA, Roberts K, Harvey LMR, Lensing SV, Cheng W, Abascal F, Leongamornlert D, Hooks Y, Mayho M, Müller-Sienerth N, Widaa S, Mincarelli L, Illing J, Peci F, Ng BL, Jarman GL, Russell AJC, Mahbubani KTA, Saeb-Parsy K, Paterson AL, Chatterjee K, Rahbari R, Bayraktar OA, Stratton MR, Campbell PJ, Tadross JA, Schoenmakers N, Martincorena I
Nature. 2026 Apr 14. doi: 10.1038/s41586-026-10493-9.

Developmental Research, Epigenetics & Aging

Fetal inflammatory signals regulate maternal investment during marsupial pregnancy
Stadtmauer DJ, Maziarz JD, Griffith OW, Wagner GP
PLoS Biol. 2026 Feb 24;24(2):e3003670. doi: 10.1371/journal.pbio.3003670.

A high-quality RNA-yielding protocol for laser capture microdissection of transplanted stem cell-derived Islets of Langerhans
Norman D, Lau J
Acta Diabetol. 2026 Feb 9. doi: 10.1007/s00592-026-02654-z.

Comparative insights into molecular pathways influencing germline development in early-divergent angiosperms
Lora J, Tucker MR, Shirley NJ, Ma C, Hormaza JI
Plant J. 2026 Jan;125(2):e70675. doi: 10.1111/tpj.70675.

Plant Research

Spongy mesophyll cell death is induced by jasmonic acid during leaf senescence in chrysanthemum
Song J, Zhang Y, Hu X, Shao Z, Song A, Jiang J, Chen S, Chen F, Wang L
Plant Cell. 2026 Mar 26:koag095. doi: 10.1093/plcell/koag095.

PtrSHR1 coordinates vascular cambium proliferation and xylem lignin biosynthesis to regulate wood formation in Populus trichocarpa
Zhao J, Chen Y, Dai X, Su L, Zhai R, Sun Y, Ji P, Wang H, Li S, Zhou C, Tobimatsu Y, Umezawa T, Chiang VL, Li W
Plant Cell. 2026 Mar 23:koag091. doi: 10.1093/plcell/koag091.

Comparative insights into molecular pathways influencing germline development in early-divergent angiosperms
Lora J, Tucker MR, Shirley NJ, Ma C, Hormaza JI
Plant J. 2026 Jan;125(2):e70675. doi: 10.1111/tpj.70675.

Forensics

Separation and identification of two-individual mixed bloodstains using human neutrophil antigen-1 antibodies
Xia X, Zheng JL, Wang SW, Liu Z, Li Z, Wang DY, Li XN, Wu X, Yao J
Int J Legal Med. 2026 Jan 27. doi: 10.1007/s00414-026-03718-z.

Invisible WItness: Innovations in the Collection of Touch DNA and the Risk of Forensic Corss Contamination
Irfan M, Haider SA, Yasir M, Akram MA, Adeel M
FORENSIC INSIGHTS AND HEALTH SCIENCES BULLETIN, Volume 3: Issue 2: 2025

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Miscellaneous

Transcobalamin 1 is a keratinocyte-derived autoantigen driving psoriasis through T helper 17 cell activation
Wang J, Li D, Zhai L, Yuan L, Zha Y, Zhang X, He Y, Qin S
Sci Adv. 2026 Apr 24;12(17):eadv6716. doi: 10.1126/sciadv.adv6716.

Laser Capture Microdissection of Paraformaldehyde-Fixed Mouse Liver Tissue for RNA Analysis
Zen WL, Zhang MM, Zeng WJ, Wang Y
JoVE, April 17th, 2026; DOI: 10.3791/69814

TYMP upregulation mediated by the hyperactivated IL-17/NF-κB1 axis promotes psoriasis through enhancing aberrant keratinization and neutrophil-mediated inflammation
Wang J, Zhou Z, Chen Y, Li Q, Zhai L, Li D, Qin S, He Y
Biol Direct. 2026 Apr 9;21(1):39. doi: 10.1186/s13062-026-00734-2.

Live Cell Culture

Deep visual proteomics uncovers nociceptor diversity and pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
Nat Commun. 2026 Apr 11;17(1):3437. doi: 10.1038/s41467-026-71418-8.

Muscle Structure and Function Recovery: Adalimumab-Calcium Channel Synergy in Post-Ischemic Stroke Sarcopenia
Qi H, Zhang XW, Zhang ZY, Ou SS, Gao YL, Tian D, Jiang YN, Min XR, Zhao AT, Zou JM, Zeng JS, Pu QY, Yang RC, Zeng N
J Cachexia Sarcopenia Muscle. 2025 Dec;16(6):e70097. doi: 10.1002/jcsm.70097.

Ultrasensitive proteomics uncovers nociceptor diversity and novel pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
bioRxiv, Sept 21, 2025

Laser microdissection (LMD) is an advanced, highly precise sample preparation technique that enables researchers to isolate pure, defined regions of interest, including single cells, from complex tissue samples. The method ensures reproducibility and accuracy for downstream analyses such as Next Generation Sequencing (NGS) including RNA-seq, and mass spectrometry for spatial genomics, spatial transcriptomics, spatial proteomics & spatial multiomics.

LMD allows non-contact dissection, isolation and gravity-assisted collection of targeted single cells, tissue compartments, cell clusters and even subcellular structures under visual control. These systems uniquely bridge microscopy and molecular biology to unlock true spatial omics insights. Combined with software-guided segmentation, Leica LMD systems enable high throughput workflows for biomarker discovery and identification of therapeutical interaction points. The study by Mund & Coscia et al., describing the Deep Visual Proteomics (DVP) workflow powered by the Leica LMD7 system, was featured on the cover of Nature Biotechnology in August 2022.  Follow-up research by Nordmann et al., published in Nature in 2024, demonstrated how DVP contributed to a life-saving therapy for toxic epidermal necrolysis (TEN), a deadly skin disease. These breakthroughs also contributed to the selection of “spatial proteomics” as Nature’s Method of the Year 2024.