レーザーマイクロダイセクション Leica LMD6 & LMD7

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This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Now there is single-cell Deep Visual Proteomics (scDVP), a technology that integrates high-content imaging, laser microdissection, and multiplexed mass spectrometry (MS) for tissue characterization at the single cell level.

Download the complete list of LMD publications in "Downloads"

DNA Research

OxPhos defects cause hypermetabolism and reduce lifespan in cells and in patients with mitochondrial diseases
Sturm G, Karan KR, Monzel AS, Santhanam B, Taivassalo T, Bris C, Ware SA, Cross M, Towheed A, Higgins-Chen A, McManus MJ, Cardenas A, Lin J, Epel ES, Rahman S, Vissing J, Grassi B, Levine M, Horvath S, Haller RG, Lenaers G, Wallace DC, St-Onge MP, Tavazoie S, Procaccio V, Kaufman BA, Seifert EL, Hirano M, Picard M
Commun Biol. 2023 Jan 12;6(1):22. doi: 10.1038/s42003-022-04303-x.

On the origin of gastric tumours: analysis of a case with intramucosal gastric carcinoma and oxyntic gland adenoma
Kumagai K, Shimizu T, Nikaido M, Hirano T, Kakiuchi N, Takeuchi Y, Minamiguchi S, Sakurai T, Teramura M, Utsumi T, Hiramatsu Y, Nakanishi Y, Takai A, Miyamoto S, Ogawa S, Seno H
J Pathol. 2023 Jan 10. doi: 10.1002/path.6050.

Mapping single-cell transcriptomes in the intra-tumoral and associated territories of kidney cancer
Li R, Ferdinand JR, Loudon KW, Bowyer GS, Laidlaw S, Muyas F, Mamanova L, Neves JB, Bolt L, Fasouli ES, Lawson ARJ, Young MD, Hooks Y, Oliver TRW, Butler TM, Armitage JN, Aho T, Riddick ACP, Gnanapragasam V, Welsh SJ, Meyer KB, Warren AY, Tran MGB, Stewart GD, Cortés-Ciriano I, Behjati S, Clatworthy MR, Campbell PJ, Teichmann SA, Mitchell TJ:
Cancer Cell. 2022 Nov 16:S1535-6108(22)00548-7. doi: 10.1016/j.ccell.2022.11.001.

RNA Research

Fear Conditioning Leads to Enduring Alterations in RNA Transcripts in Hippocampal Neuropil that are Dependent on EphB2 Forward Signaling
Jana S, Dines M, Lalzar M, Lamprecht R
Mol Neurobiol. 2023 Jan 13. doi: 10.1007/s12035-022-03191-w.

Transcriptional regulatory network during axonal regeneration of dorsal root ganglion neurons: laser-capture microdissection and deep sequencing
Zhao LL, Zhang T, Huang WX, Gui TT, Gu XS
Neural Regen Res 18(0):000-000. (Jan 2023)

Precise measurement of gene expression changes in mouse brain areas denervated by injury
Schlaudraff J, Paul MH, Deller T, Del Turco D
Sci Rep. 2022 Dec 29;12(1):22530. doi: 10.1038/s41598-022-26228-5.

Proteomics, Lipidomics, Metabolomics

MALDI-IHC-Guided In-Depth Spatial Proteomics: Targeted and Untargeted MSI Combined
Claes BSR, Krestensen KK, Yagnik G, Grgic A, Kuik C, Lim MJ, Rothschild KJ, Vandenbosch M, Heeren RMA
Anal Chem. 2023 Jan 13. doi: 10.1021/acs.analchem.2c04220.

Spatial proteomics in three-dimensional intact specimens.
Bhatia HS, Brunner AD, Öztürk F, Kapoor S, Rong Z, Mai H, Thielert M, Ali M, Al-Maskari R, Paetzold JC, Kofler F, Todorov MI, Molbay M, Kolabas ZI, Negwer M, Hoeher L, Steinke H, Dima A, Gupta B, Kaltenecker D, Caliskan ÖS, Brandt D, Krahmer N, Müller S, Lichtenthaler SF, Hellal F, Bechmann I, Menze B, Theis F, Mann M, Ertürk A
Cell. 2022 Dec 22;185(26):5040-5058.e19. doi: 10.1016/j.cell.2022.11.021

Integrated multi-omic analysis of low-grade ovarian serous carcinoma collected from short and long-term survivors
Wong KK, Bateman NW, Ng CW, Tsang YTM, Sun CS, Celestino J, Nguyen TV, Malpica A, Hillman RT, Zhang J, Futreal PA, Rojas C, Conrads KA, Hood BL, Dalgard CL, Wilkerson MD, Phippen NT, Conrads TP, Maxwell GL, Sood AK, Gershenson DM
J Transl Med. 2022 Dec 17;20(1):606. doi: 10.1186/s12967-022-03820-x.

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
Mund A, Coscia F, Kriston A, Hollandi R, Kovacs F, Brunner A-D, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
Nat Biotechnol (2022). doi.org/10.1038/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Fluorescence Applications

Transcriptional regulatory network during axonal regeneration of dorsal root ganglion neurons: laser-capture microdissection and deep sequencing
Zhao LL, Zhang T, Huang WX, Gui TT, Gu XS
Neural Regen Res 18(0):000-000. (Jan 2023)

Amino acid transporter SLC38A5 regulates developmental and pathological retinal angiogenesis
Wang Z, Yemanyi F, Blomfield AK, Bora K, Huang S, Liu CH, Britton WR, Cho SS, Tomita Y, Fu Z, Ma JX, Li WH, Chen J
Elife. 2022 Dec 1;11:e73105. doi: 10.7554/eLife.73105.

Glial scar survives until the chronic phase by recruiting scar-forming astrocytes after spinal cord injury
​​​Tamaru T, Kobayakawa K, Saiwai H, Konno D, Kijima K, Yoshizaki S, Hata K, Iura H, Ono G, Haruta Y, Kitade K, Iida KI, Kawaguchi KI, Matsumoto Y, Kubota K, Maeda T, Okada S, Nakashima Y:
Exp Neurol. 2022 Nov 3:114264. doi: 10.1016/j.expneurol.2022.114264.

Cancer Research

On the origin of gastric tumours: analysis of a case with intramucosal gastric carcinoma and oxyntic gland adenoma
Kumagai K, Shimizu T, Nikaido M, Hirano T, Kakiuchi N, Takeuchi Y, Minamiguchi S, Sakurai T, Teramura M, Utsumi T, Hiramatsu Y, Nakanishi Y, Takai A, Miyamoto S, Ogawa S, Seno H
J Pathol. 2023 Jan 10. doi: 10.1002/path.6050.

Identification of upstream immunoregulators that target protein co-expression networks significantly associated with early-stage micropapillary/solid predominant lung adenocarcinomas
Nishimura T, Nakamura H, Fujii K, Sakai H, Naruki S, Furuya N, Kato H, Saji H
Research Square, December 20th 2022, DOI: doi.org/10.21203/rs.3.rs-2377717/v1

Integrated multi-omic analysis of low-grade ovarian serous carcinoma collected from short and long-term survivors
Wong KK, Bateman NW, Ng CW, Tsang YTM, Sun CS, Celestino J, Nguyen TV, Malpica A, Hillman RT, Zhang J, Futreal PA, Rojas C, Conrads KA, Hood BL, Dalgard CL, Wilkerson MD, Phippen NT, Conrads TP, Maxwell GL, Sood AK, Gershenson DM
J Transl Med. 2022 Dec 17;20(1):606. doi: 10.1186/s12967-022-03820-x.

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
Mund A, Coscia F, Kriston A, Hollandi R, Kovacs F, Brunner A-D, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
Nat Biotechnol (2022). doi.org/10.1038/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Neurosciences

Fear Conditioning Leads to Enduring Alterations in RNA Transcripts in Hippocampal Neuropil that are Dependent on EphB2 Forward Signaling
Jana S, Dines M, Lalzar M, Lamprecht R
Mol Neurobiol. 2023 Jan 13. doi: 10.1007/s12035-022-03191-w.

Early deficits in GABA inhibition parallels an increase in L-type Ca2+ currents in the jaw motor neurons of SOD1G93A mouse model for ALS
Venugopal S, Ghulam-Jhelani Z, Ahn IS, Yang X, Wiedau M, Simmons D, Chandler SH
Neurobiol Dis. 2023 Jan 6:105992. doi: 10.1016/j.nbd.2023.105992.

Transcriptional regulatory network during axonal regeneration of dorsal root ganglion neurons: laser-capture microdissection and deep sequencing
Zhao LL, Zhang T, Huang WX, Gui TT, Gu XS
Neural Regen Res 18(0):000-000. (Jan 2023)

Clinical/Pathology/Immunology Research Applications

OxPhos defects cause hypermetabolism and reduce lifespan in cells and in patients with mitochondrial diseases
Sturm G, Karan KR, Monzel AS, Santhanam B, Taivassalo T, Bris C, Ware SA, Cross M, Towheed A, Higgins-Chen A, McManus MJ, Cardenas A, Lin J, Epel ES, Rahman S, Vissing J, Grassi B, Levine M, Horvath S, Haller RG, Lenaers G, Wallace DC, St-Onge MP, Tavazoie S, Procaccio V, Kaufman BA, Seifert EL, Hirano M, Picard M
Commun Biol. 2023 Jan 12;6(1):22. doi: 10.1038/s42003-022-04303-x.

Pathological characteristics of light chain crystalline podocytopathy
Nasr SH, Kudose S, Javaugue V, Harel S, Said SM, Pascal V, Stokes MB,Vrana JA, Dasari S, Theis JD, Osuchukwu GA, Sathick IJ, Das A, Kashkouli A, Suchin EJ, Liss Y,Suldan Z, Verine J, Arnulf B, Talbot A, Sethi S, Zaidan M, Goujon JM, Valeri AM, Mcphail ED, Sirac C,Leung N, Bridoux F, D’Agati VD
Kidney International (2023), doi: doi.org/10.1016/j.kint.2022.11.026.

VPAC1 and VPAC2 receptor deficiencies negatively influence pregnancy outcome through distinct and overlapping modulations of immune, trophoblast and vascular functions
Calo G, Hauk V, Vota D, Van C, Condro M, Gallino L, Ramhorst R, Waschek J, Leirós CP:
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2022, 166593, ISSN 0925-4439,

Developmental Research, Epigenetics  & Aging

Cell-type-specific PtrWOX4a and PtrVCS2 form a regulatory nexus with a histone modification system for stem cambium development in Populus trichocarpa
Dai X, Zhai R, Lin J, Wang Z, Meng D, Li M, Mao Y, Gao B, Ma H, Zhang B, Sun Y, Li S, Zhou C, Lin YJ, Wang JP, Chiang VL, Li W
Nat Plants. 2023 Jan 9. doi: 10.1038/s41477-022-01315-7.

Amino acid transporter SLC38A5 regulates developmental and pathological retinal angiogenesis
Wang Z, Yemanyi F, Blomfield AK, Bora K, Huang S, Liu CH, Britton WR, Cho SS, Tomita Y, Fu Z, Ma JX, Li WH, Chen J
Elife. 2022 Dec 1;11:e73105. doi: 10.7554/eLife.73105.

VPAC1 and VPAC2 receptor deficiencies negatively influence pregnancy outcome through distinct and overlapping modulations of immune, trophoblast and vascular functions
Calo G, Hauk V, Vota D, Van C, Condro M, Gallino L, Ramhorst R, Waschek J, Leirós CP
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2022, 166593, ISSN 0925-4439,

Plant Research

Control of plant cell growth and proliferation by MO25A, a conserved major component of the Mammalian Sterile20-like kinase pathway
Ta KN, Yoshida MW, Tezuka T, Shimizu-Sato S, Nosaka-Takahashi M, Toyoda A, Suzuki T, Goshima G, Sato Y
Plant Cell Physiol. 2023 Jan 13:pcad005. doi: 10.1093/pcp/pcad005.

Cell-type-specific PtrWOX4a and PtrVCS2 form a regulatory nexus with a histone modification system for stem cambium development in Populus trichocarpa
Dai X, Zhai R, Lin J, Wang Z, Meng D, Li M, Mao Y, Gao B, Ma H, Zhang B, Sun Y, Li S, Zhou C, Lin YJ, Wang JP, Chiang VL, Li W
Nat Plants. 2023 Jan 9. doi: 10.1038/s41477-022-01315-7.

An efficient semi–in vivo zygotic embryogenesis system in Arabidopsis
Li J, Li SS, Liu Y, Tang WY, Liu XF, Meng CF, Zhang LY, Sun MX
In Vitro Cell.Dev.Biol.-Plant, 27. Dec 2022

Forensics

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
Sun M, Zheng J, Wnag B and Yao J:
BioMed Research International, Volume 2021 | Article ID 7269237

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Further information on Leica LMD impact at forensics: 
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

MALDI-IHC-Guided In-Depth Spatial Proteomics: Targeted and Untargeted MSI Combined
Claes BSR, Krestensen KK, Yagnik G, Grgic A, Kuik C, Lim MJ, Rothschild KJ, Vandenbosch M, Heeren RMA
Anal Chem. 2023 Jan 13. doi: 10.1021/acs.analchem.2c04220.

The full-length Auxin Response Factor 8 isoform ARF8.1 controls pollen cell wall formation and directly regulates TDF1, AMS and MS188 expression
Ghelli R, Brunetti P, Marzi D, Cecchetti V, Costantini M, Lanzoni-Rossi M, Scaglia Linhares F, Costantino P, Cardarelli M
Plant J. 2023 Jan 3. doi: 10.1111/tpj.16089.

FMR1 deletion in rats induces hyperactivity with no changes in striatal dopamine transporter availability
D'Elia A, Schiavi S, Manduca A, Rava A, Buzzelli V, Ascone F, Orsini T, Putti S, Soluri A, Galli F, Soluri A, Mattei M, Cicconi R, Massari R, Trezza V
Sci Rep. 2022 Dec 29;12(1):22535. doi: 10.1038/s41598-022-26986-2.

Live Cell Culture

An efficient semi–in vivo zygotic embryogenesis system in Arabidopsis
Li J, Li SS, Liu Y, Tang WY, Liu XF, Meng CF, Zhang LY, Sun MX
In Vitro Cell.Dev.Biol.-Plant, 27. Dec 2022

Direct Reprogramming of Mouse Subchondral Bone Osteoblasts into Chondrocyte-Like Cells
Li M, Zhang L, Li J, and Zhu Q
Biomedicines 2022, 10, 2582.

Heterotypic cell-in-cell structures between cancer and NK cells are associated with enhanced anticancer drug resistance
Choe YJ, Min JY, Lee H, Lee SY, Kwon J, Kim HJ, Lee J, Kim HM, Park HS, Cho MY, Hyun JY, Kim HM, Chung YH, Ha SK, Jeong HG, Choi I, Kim TD, Hong KS, and Han EH
iScience 25, 105017 September 16, 2022

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