Leica LMD6 & LMD7 レーザーマイクロダイセクション

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Now there is single-cell Deep Visual Proteomics (scDVP), a technology that integrates high-content imaging, laser microdissection, and multiplexed mass spectrometry (MS) for tissue characterization at the single cell level.

Download the complete list of LMD publications

Single cell applications

Research progress and application of single-cell sequencing in head and neck malignant tumors
Qu S, Gong M, Deng Y, Xiang Y, Ye D
Cancer Gene Ther. 2023 Nov 15. doi: 10.1038/s41417-023-00691-2.

Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Krishnan R, Shimizu A, Metousis A, Kenny H, Mendoza R, Nordmann TM, Rauch S, Kelliher L, Heide J, Rosenberger FA, Bilecz A, Borrego SN, Strauss MT, Thielert M, Rodriguez E, Müller-Reif, JB, Chen M, Yamada SD, Mund A, Lastra RR, Mann M, and Lengyei E
medRxiv preprint, November 13, 2023

Single-cell spatial proteomics identifies the JAK/STAT pathway as an actionable therapeutic target in lethal cutaneous drug reactions
Nordmann TM, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler P-C, Sinha A, Metousis A, Rosenberger FA, Zwiebel M, Satoh TK, Anzengruber F, Tanzer MC, Saito Y, Gong T, Thielert M, Kimura H, Silke N, Rodriguez EH, Gaetana R, Nguyen HH, Gross A, Levesque MP, Murray PJ, Ingen-Housz-Oro S, Mund A, Abe R, Silke J, Ji C, French LE, Mann M
edRxiv preprint,  November 12, 2023

Investigating modifiers that can regulate selective vulnerability in mouse models of spinal muscular atrophy
Woschitz V:
Thesis, 2023

DNA Research

DEEP MOLECULAR TRACKING OVER THE 12-YEAR DEVELOPMENT OF ENDOMETRIAL CANCER FROM HYPERPLASIA IN A SINGLE PATIENT - Endometrial hyperplasia to cancer
Reid K, Camacho-Vanegas O, Pandya D, Camacho SC, Qiao RF, Kalir T, Padron MM, Beddoe A-M, Dottino P, and Martignetti JA
Cold Spring Harbor Laboratory Press, Oct 30th 2023, from molecularcasestudies.cshlp.org 

Strategic approach to heterogeneity analysis of cutaneous adnexal carcinomas using computational pathology and genomics
Nishimura T, Eijitsu R, Inoue S, Kawazu M, Ueno T, Namikawa K, Takahashi A, OgataD, Yoshida A, Yamazaki N, Mano H, Yatabe Y, Mori T
JID Innovations, Skin Science from Molecules to Population Health, 05 Sep 2023 

Molecular biological analysis revealed a case of synchronous endometrial and ovarian cancer with different histological grade as metastatic ovarian cancer from endometrial cancer: Case report and review of literature
Yumisashi R, Saito R, Togami S, Kobayashi Y, Kitazono I, Tanimoto A, Kobayashi H
J Obstet Gynaecol Res. 2023 Aug 21. doi: 10.1111/jog.15773.

RNA Research

Uterine scars after caesarean delivery: From histology to the molecular and ultrastructural level
Paping A, Basler C, Ehrlich L, Fasting C, Melchior K, Ziska T, Thiele M, Duda GN, Timm S, Ochs M, Rancourt RC, Henrich W, Braun T
Wound Repair Regen. 2023 Nov 13. doi: 10.1111/wrr.13127.

Angiotensin II type 1 receptor-associated protein deletion combined with angiotensin II stimulation accelerates the development of diabetic kidney disease in mice on a C57BL/6 strain
Taguchi S, Azushima K, Yamaji T, Suzuki T, Abe E, Tanaka S, Hirota K, Tsukamoto S, Morita R, Kobayashi R, Kinguchi S, Yamashita A, Wakui H, Tamura K
Hypertens Res. 2023 Nov 13. doi: 10.1038/s41440-023-01496-4.

An intact pituitary vasopressin system is critical for building a robust circadian clock in the suprachiasmatic nucleus
Yamaguchi Y, Maekawa Y, Kabashima K, Mizuno T, Tainaka M, Suzuki T, Dojo K, Tominaga T, Kuroiwa S, Masubuchi S, Doi M, Tominaga K, Kobayashi K, Yamagata S, Itoi K, Abe M, Schwartz WJ, Sakimura K, Okamura H
Proc Natl Acad Sci U S A. 2023 Oct 24;120(43):e2308489120. doi: 10.1073/pnas.2308489120.

Proteomics, Lipidomics, Metabolomics

Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Krishnan R, Shimizu A, Metousis A, Kenny H, Mendoza R, Nordmann TM, Rauch S, Kelliher L, Heide J, Rosenberger FA, Bilecz A, Borrego SN, Strauss MT, Thielert M, Rodriguez E, Müller-Reif, JB, Chen M, Yamada SD, Mund A, Lastra RR, Mann M, and Lengyei E
medRxiv preprint, November 13, 2023

Single-cell spatial proteomics identifies the JAK/STAT pathway as an actionable therapeutic target in lethal cutaneous drug reactions
Nordmann TM, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler P-C, Sinha A, Metousis A, Rosenberger FA, Zwiebel M, Satoh TK, Anzengruber F, Tanzer MC, Saito Y, Gong T, Thielert M, Kimura H, Silke N, Rodriguez EH, Gaetana R, Nguyen HH, Gross A, Levesque MP, Murray PJ, Ingen-Housz-Oro S, Mund A, Abe R, Silke J, Ji C, French LE, Mann M
edRxiv preprint,  November 12, 2023

In situ profiling reveals metabolic alterations in the tumor microenvironment of ovarian cancer after chemotherapy
Corvigno S, Badal S, Spradlin ML, Keating M, Pereira I, Stur E, Bayraktar E, Foster KI, Bateman NW, Barakat W, Darcy KM, Conrads TP, Maxwell GL, Lorenzi PL, Lutgendorf SK, Wen Y, Zhao L, Thaker PH, Goodheart MJ, Liu J, Fleming N, Lee S, Eberlin LS, Sood AK
NPJ Precis Oncol. 2023 Nov 3;7(1):115. doi: 10.1038/s41698-023-00454-0.

Highlight 2022: Deep Visual Proteomics (DVP)
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/

Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Fluorescence Applications

Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Krishnan R, Shimizu A, Metousis A, Kenny H, Mendoza R, Nordmann TM, Rauch S, Kelliher L, Heide J, Rosenberger FA, Bilecz A, Borrego SN, Strauss MT, Thielert M, Rodriguez E, Müller-Reif, JB, Chen M, Yamada SD, Mund A, Lastra RR, Mann M, and Lengyei E
medRxiv preprint, November 13, 2023

Protein reservoirs of seeds are amyloid composites employed differentially for germination and seedling emergence
Sinha N, Zahra T, Gahane AY, Rout B, Bhattacharya A, Basu S, Chakrabarti A, Thakur AK
Plant J. 2023 Oct;116(2):329-346. doi: 10.1111/tpj.16429.

Root Primordium Defective 1 Encodes an Essential PORR Protein Required for the Splicing of Mitochondria Encoded Group II Introns and for Respiratory Complex I Biogenesis
Edris R, Sultan LD, Best C, Mizrahi R, Weinstein O, Chen S, Kamennaya NA, Keren N, Zer H, Zhu H, Ostersetzer-Biran O
Plant Cell Physiol. 2023 Sep 13:pcad101. doi: 10.1093/pcp/pcad101.

Cancer Research

Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Krishnan R, Shimizu A, Metousis A, Kenny H, Mendoza R, Nordmann TM, Rauch S, Kelliher L, Heide J, Rosenberger FA, Bilecz A, Borrego SN, Strauss MT, Thielert M, Rodriguez E, Müller-Reif, JB, Chen M, Yamada SD, Mund A, Lastra RR, Mann M, and Lengyei E
medRxiv preprint, November 13, 2023

In situ profiling reveals metabolic alterations in the tumor microenvironment of ovarian cancer after chemotherapy
Corvigno S, Badal S, Spradlin ML, Keating M, Pereira I, Stur E, Bayraktar E, Foster KI, Bateman NW, Barakat W, Darcy KM, Conrads TP, Maxwell GL, Lorenzi PL, Lutgendorf SK, Wen Y, Zhao L, Thaker PH, Goodheart MJ, Liu J, Fleming N, Lee S, Eberlin LS, Sood AK
NPJ Precis Oncol. 2023 Nov 3;7(1):115. doi: 10.1038/s41698-023-00454-0.

The murine metastatic microenvironment of experimental brain metastases of breast cancer differs by host age in vivo: a proteomic study
Hunt AL, Khan I, Wu AML, Makohon-Moore SC, Hood BL, Conrads KA, Abulez T, Ogata J, Mitchell D, Gist G, Oliver J, Wei D, Chung MA, Rahman S, Bateman NW, Zhang W, Conrads TP, Steeg PS
Clin Exp Metastasis. 2023 Nov 2. doi: 10.1007/s10585-023-10233-7.

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
Mund A, Coscia F, Kriston A, Hollandi R, Kovacs F, Brunner A-D, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
Nat Biotechnol (2022). doi.org/10.1038/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Neurosciences

Investigating modifiers that can regulate selective vulnerability in mouse models of spinal muscular atrophy
Woschitz V:
Thesis, 2023

NR1 Splicing Variant NR1a in Cerebellar Granule Neurons Constitutes a Better Motor Learning in the Mouse
Tan T, Jiang L, He Z, Ding X, Xiong X, Tang M, Chen Y, Tang Y
Cerebellum. 2023 Oct 25. doi: 10.1007/s12311-023-01614-5.

An intact pituitary vasopressin system is critical for building a robust circadian clock in the suprachiasmatic nucleus
Yamaguchi Y, Maekawa Y, Kabashima K, Mizuno T, Tainaka M, Suzuki T, Dojo K, Tominaga T, Kuroiwa S, Masubuchi S, Doi M, Tominaga K, Kobayashi K, Yamagata S, Itoi K, Abe M, Schwartz WJ, Sakimura K, Okamura H
Proc Natl Acad Sci U S A. 2023 Oct 24;120(43):e2308489120. doi: 10.1073/pnas.2308489120.

Clinical/Pathology/Immunology Research Applications

Angiotensin II type 1 receptor-associated protein deletion combined with angiotensin II stimulation accelerates the development of diabetic kidney disease in mice on a C57BL/6 strain
Taguchi S, Azushima K, Yamaji T, Suzuki T, Abe E, Tanaka S, Hirota K, Tsukamoto S, Morita R, Kobayashi R, Kinguchi S, Yamashita A, Wakui H, Tamura K
Hypertens Res. 2023 Nov 13. doi: 10.1038/s41440-023-01496-4.

Single-cell spatial proteomics identifies the JAK/STAT pathway as an actionable therapeutic target in lethal cutaneous drug reactions
Nordmann TM, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler P-C, Sinha A, Metousis A, Rosenberger FA, Zwiebel M, Satoh TK, Anzengruber F, Tanzer MC, Saito Y, Gong T, Thielert M, Kimura H, Silke N, Rodriguez EH, Gaetana R, Nguyen HH, Gross A, Levesque MP, Murray PJ, Ingen-Housz-Oro S, Mund A, Abe R, Silke J, Ji C, French LE, Mann M
medRxiv preprint,  November 12, 2023

An extra-erythrocyte role of haemoglobin body in chondrocyte hypoxia adaption
Zhang F, Zhang B, Wang Y, Jiang R, Liu J, Wei Y, Gao X, Zhu Y, Wang X, Sun M, Kang J, Liu Y, You G, Wei D, Xin J, Bao J, Wang M, Gu Y, Wang Z, Ye J, Guo S, Huang H, Sun Q
Nature. 2023 Oct 4. doi: 10.1038/s41586-023-06611-6.

Developmental Research, Epigenetics  & Aging

Protein reservoirs of seeds are amyloid composites employed differentially for germination and seedling emergence
Sinha N, Zahra T, Gahane AY, Rout B, Bhattacharya A, Basu S, Chakrabarti A, Thakur AK
Plant J. 2023 Oct;116(2):329-346. doi: 10.1111/tpj.16429.

Mast Cell Dynamics in the Ovary Are Governed by GnRH and Prolactin
Laoharatchatathanin T, Rieanrakwong D, Hatsugai Y, Terashima R, Yonezawa T, Kurusu S, Kawaminami M
Endocrinology. 2023 Sep 23;164(11):bqad144. doi: 10.1210/endocr/bqad144.

FSHR-mTOR-HIF1 signaling alleviates mouse follicles from AMPK-induced atresia
Liu L, Hao M, Zhang J, Chen Z, Zhou J, Wang C, Zhang H, Wang J
Cell Rep. 2023 Sep 19;42(10):113158. doi: 10.1016/j.celrep.2023.113158.

Plant Research

Protein reservoirs of seeds are amyloid composites employed differentially for germination and seedling emergence
Sinha N, Zahra T, Gahane AY, Rout B, Bhattacharya A, Basu S, Chakrabarti A, Thakur AK
Plant J. 2023 Oct;116(2):329-346. doi: 10.1111/tpj.16429.

NanoLC-MS/MS protein analysis on laser-microdissected wheat endosperm tissues: A comparison between aleurone, sub-aleurone and inner endosperm
Hermans W, Geisslitz S, De Bondt Y, Langenaeken NA, Scherf KA, Courtin CM
Food Chemistry, 12 Oct 2023, 137735

Root Primordium Defective 1 Encodes an Essential PORR Protein Required for the Splicing of Mitochondria Encoded Group II Introns and for Respiratory Complex I Biogenesis
Edris R, Sultan LD, Best C, Mizrahi R, Weinstein O, Chen S, Kamennaya NA, Keren N, Zer H, Zhu H, Ostersetzer-Biran O
Plant Cell Physiol. 2023 Sep 13:pcad101. doi: 10.1093/pcp/pcad101.

Forensics

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
Sun M, Zheng J, Wnag B and Yao J:
BioMed Research International, Volume 2021 | Article ID 7269237

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Further information on Leica LMD impact at forensics: 
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Research progress and application of single-cell sequencing in head and neck malignant tumors
Qu S, Gong M, Deng Y, Xiang Y, Ye D
Cancer Gene Ther. 2023 Nov 15. doi: 10.1038/s41417-023-00691-2.

Routine Workflow of Spatial Proteomics on Micro-formalin-Fixed Paraffin-Embedded Tissues
Chen H, Zhang Y, Zhou H, Chen W, Peng J, Feng Y, Fan L, Li J, Zi J, Ren Y, Li Q, Liu S
Anal Chem. 2023 Nov 3. doi: 10.1021/acs.analchem.3c03848.

Forsythia suspensa (Thunb.) Vahl extract ameliorates ulcerative colitis via inhibiting NLRP3 inflammasome activation through the TLR4/MyD88/NF‐κB pathway
Tong X, Chen L, He S, Liu S, Yao J, Shao Z, YeY, Yao S, Lin Z, Zuo J
Immun Inflamm Dis.01 Nov 2023;11:e1069.

Live Cell Culture

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416

An efficient semi–in vivo zygotic embryogenesis system in Arabidopsis
Li J, Li SS, Liu Y, Tang WY, Liu XF, Meng CF, Zhang LY, Sun MX
In Vitro Cell.Dev.Biol.-Plant, 27. Dec 2022