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光電子相関顕微鏡 EM Cryo CLEM

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  • Front Microbiol. 2017 Apr 11;8:596; Pleomorphism and Viability of the Lyme Disease Pathogen Borrelia burgdorferi Exposed to Physiological Stress Conditions: A Correlative Cryo-Fluorescence and Cryo-Scanning Electron Microscopy Study; Vancová M, Rudenko N, Vaněček J, Golovchenko M, Strnad M, Rego ROM, Tichá L, Grubhoffer L, Nebesářová J.
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387694/
  • Nat Protoc. 2017 Jan;12(1):150-167; Correlated fluorescence microscopy and cryo-electron tomography of virus-infected or transfected mammalian cells; Hampton CM, Strauss JD, Ke Z, Dillard RS, Hammonds JE, Alonas E, Desai TM, Marin M, Storms RE, Leon F, Melikyan GB, Santangelo PJ, Spearman PW, Wright ER.
    https://www.nature.com/articles/nprot.2016.168
  • Methods Cell Biol. 2017;140:321-333; Matrix MAPS-an intuitive software to acquire, analyze, and annotate light microscopy data for CLEM; Schorb M, Sieckmann F.
    http://www.sciencedirect.com/science/article/pii/S0091679X17300560
  • Methods Cell Biol. 2017;140:303-320; triCLEM: Combining high-precision, room temperature CLEM with cryo-fluorescence microscopy to identify very rare events; Ader NR, Kukulski W.
    http://www.sciencedirect.com/science/article/pii/S0091679X17300535
  • Methods Cell Biol. 2017;140:85-103; A new method for cryo-sectioning cell monolayers using a correlative workflow; Kolovou A, Schorb M, Tarafder A, Sachse C, Schwab Y, Santarella-Mellwig R.
    http://www.sciencedirect.com/science/article/pii/S0091679X17300559
  • New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy tomography, Martin Schorba, b, Leander Gaechterc, Ori Avinoama, Frank Sieckmannd, Mairi Clarkea, Cecilia Bebeacuaa, e, Yury S. Bykova, Andreas F.-P. Sonnena, f, Reinhard Lihlg, John A.G. Briggsa, e, f,, a Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany, b Electron Microscopy Core Facility, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany, c Leica Microsystems (Schweiz) AG, Max Schmidheiny-Strasse 201, 9435 Heerbrugg, Switzerland, d Leica Microsystems GmbH, Am Friedensplatz 3, 68165 Mannheim, Germany, e Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany, f Molecular Medicine Partnership Unit, EMBL/Universitätsklinikum Heidelberg, Heidelberg, Germany, g Leica Mikrosysteme GmbH, Hernalser Hauptstraße 219, 1170 Vienna, Austria, http://www.sciencedirect.com/science/article/pii/S1047847716301356
  • Correlative cryo-fluorescence and cryo-scanning electron microscopy as a straightforward tool to study host-pathogen interactions, Martin Strnad1,2, Jana Elsterová1,2,3, Jana Schrenková1,2, Marie Vancová1,2, Ryan O. M. Rego1,Libor Grubhoffer1,2 & Jana Nebesářová1,2,4, 1Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice CZ- 37005, Czech Republic. 2Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760, České Budějovice CZ-37005, Czech Republic. 3Department of Virology, Veterinary Research Institute, Brno CZ-62100, Czech Republic. 4Faculty of Science, Charles University in Prague, Viničná 1594/7, Praha CZ-12800, Czech Republic. Correspondence and requests for materials should be addressed to M.S. (email: martin.strnad.cze(at)gmail.com),
    https://www.nature.com/articles/srep18029
  • Metskas LA, Briggs JAG
    Fluorescence-Based Detection of Membrane Fusion State on a Cryo-EM Grid using Correlated Cryo-Fluorescence and Cryo-Electron Microscopy
    Microscopy and Microanalysis. 2019 August; 25(4):942-949.
    https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/fluorescencebased-detection-of-membrane-fusion-state-on-a-cryoem-grid-using-correlated-cryofluorescence-and-cryoelectron-microscopy/4933BFD9777A1E735075BFE5A60DFC90#fndtn-information