Leica LMD6 & LMD7 レーザーマイクロダイセクション

This curated list features recent scientific publications that have employed Leica’s LMD6 and LMD7 laser microdissection systems in key areas such as cancer research, neuroscience, molecular pathology, forensics, and spatial omics. It is updated regularly to reflect the latest scientific publications utilizing laser microdissection applications.

Explore the full list below to see how Leica LMD systems are enabling scientific success across the globe.

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Single Cell Applications

Characterisation of the megakaryocyte proteome in patients with Philadelphia negative myeloproliferative neoplasms
Raman I, Bennett C, Pettikiriarachchi A, Ataide R, Zhu Y, Tsui E, Juneja S, Emery-Corbin S, Spall S, Yousef J, Dagley L, Yannakou C, Pasricha SR
Blood 146 (2025) 5539–5540 (Poster)

Clinical application of single-cell RNA sequencing in disease and therapy
Nadukkandy AS, Kalaiselvan S, Lin L, Luo Y
Clin Transl Med. 2025 Nov;15(11):e70512. doi: 10.1002/ctm2.70512.

Neuronal gene profiling of tau oligomer-bearing cholinergic nucleus basalis neurons during the onset of Alzheimer's disease
Kara B, Beck JS, Fu Z, Hickey SL, Kanaan NM, Mufson EJ, Ginsberg SD, Counts SE
Acta Neuropathol Commun. 2025 Oct 27;13(1):218. doi: 10.1186/s40478-025-02080-2.

Spatial Genomics (DNA-Reserach)

Genomic profiling of meiotic errors and early malignant transformation events in ovarian mature teratoma
Takahashi K, Tamura R, Suda K, Yachida N, Yamaguchi M, Mathew RM, Okuda S, Yoshihara K, Nakaoka 
Reproduction, xaaf019, Jan 15th 2026

Laser Microdissection and Near Single-Cell Whole Genome Amplification
Cruz-Flores R, Cáceres-Martínez J, Dhar AK
Methods Mol Biol. 2026;3003:165-173. doi: 10.1007/978-1-0716-5060-8_12.

Morphology shapes microbial ecosystems and carbon cycling within cryoconite holes on a Greenland outlet glacier
Takeuchi N, Murakami T, Ishiwatari K, Watanabe A, Segawa T
Commun Earth Environ (2025)

Spatial Transcriptomics (RNA Research)

Neural crest cell-derived DKK1 and NEDD4 modulate Wnt signalling in the second heart field to orchestrate outflow tract development
Wiszniak S, Alankarage D, Lohraseb I, Marchant C, Secker G, Domingo D, Hartmann J, Zhang T, Parker W, Toubia J, White M, Piltz S, Tondl M, Giannoulatou E, Winlaw D, Blue GM; Congenital Heart Disease Synergy Group; Tam PPL, Thomas P, Harvey N, Dunwoodie SL, Schwarz Q
Nat Commun. 2026 Jan 22. doi: 10.1038/s41467-026-68459-4.

IIIG9 and PP1α form a protein complex in the adherens junctions of polarized ependymal and MDCK cells
Oviedo MJ, Ramírez E, De Lima I, Grondona JM, Cifuentes M, Martínez F, Barahona MJ, Nualart F, Salazar K
Fluids Barriers CNS. 2026 Jan 21;23(1):12. doi: 10.1186/s12987-026-00762-0.

Comparative insights into molecular pathways influencing germline development in early-divergent angiosperms
Lora J, Tucker MR, Shirley NJ, Ma C, Hormaza JI
Plant J. 2026 Jan;125(2):e70675. doi: 10.1111/tpj.70675.

Proteomics, Lipidomics, Metabolomics

Light Chain Cast Nephropathy: The Relationship between Tamm-Horsfall Protein Immunoreactivity and Clinical Pathological Features and Prognosis
Wang MY, Yong ZH, Wang S, Wang SX, Zhou XJ, Dong YJ, Zhao MH, Yu XJ, Zhou FD
Kidney Dis (2026)

Discovery and development of a new oxazolidinone with reduced toxicity for the treatment of tuberculosis
Crowley BM, Boshoff HI, Boving A, Tan VY, Zhu J, Hoyt F, Miller RR, Ehrhart J, Boyce CW, Young K, Nantermet PG, Su J, Yang L, Painter RE, Corcoran EB, Hoar JL, Oh S, Holtzman DL, Levi M, Anderson A, Otieno MA, Zimmerman M, Kaya F, Massoudi LM, Ramey ME, Bauman AA, Lenaerts AJ, Roberston GT, Dartois V, Wells CD, Barry CE 3rd, Olsen DB
Nat Med. 2026 Jan 13. doi: 10.1038/s41591-025-04164-x.

Cognitively Healthy Centenarian Brains resist Tau Accumulation and exhibit lower APOE, early proteasome response and maintained β-oxidation
Hulsman M, Ganz AB, Zhang M, Koopmans F, Li KW, Miedema SSM, Rohde SK, Luimes MC, Morrema THJ, Rozemulle AJM, Scheltens P, Hoozemans JJM, Reinders MJT, Smit AB, and Holstege H
bioRxiv, 8th Jan 2026

Deep Visual Proteomics (DVP) related

Advances and Applications of Spatial Proteomics: From Organellar Maps to Clinical Translation
Bernardini C, Däther M, Traube FR
Chembiochem. 2026 Jan;27(1):e202500616. doi: 10.1002/cbic.202500616.

High-resolution biology and the evolving nature of skin biopsy analysis in translational dermatology
McGrath JA
British Journal of Dermatology, ljag014, 9th Jan 2026

Characterisation of the megakaryocyte proteome in patients with Philadelphia negative myeloproliferative neoplasms
Raman I, Bennett C, Pettikiriarachchi A, Ataide R, Zhu Y, Tsui E, Juneja S, Emery-Corbin S, Spall S, Yousef J, Dagley L, Yannakou C, Pasricha SR
Blood 146 (2025) 5539–5540 (Poster)

Fluorescence Applications

Similar growth potentials of cyanobacteria and algae explain their coexistence in desert soils
Kyi KM, Levintal E, A Kamennaya N
Sci Rep. 2025 Dec 1;16(1):830. doi: 10.1038/s41598-025-30489-1.

Proteomic profile of Laser-dissected Motoneurons and Ependymal Cell Layer and of Dorsal Root Ganglia after Spinal Cord Injury in Rat
Gajewska-Woźniak O, Pytyś A, Wójtowicz T, Serwa R, Radwanska K, Skup M
Reasearch Square, November 2025

TMPRSS11B promotes an acidified microenvironment and immune suppression in squamous lung cancer
Sunil HS, Clemenceau JR, Grichuk A, Barnfather I, Nakkireddy SR, Izzo L, Feng Q, Hartnett W, Evers BM, Thomas L, Subramaniyan I, Li L, Putnam WC, Hepensteil S, Zhu J, Updegraff B, Minna JD, DeBerardinis RJ, Hwang TH, Gao J, Oliver TG, O'Donnell KA
EMBO Rep. 2025 Nov 10. doi: 10.1038/s44319-025-00631-1. PMID: 41214366

Cancer Research

Discovery and development of a new oxazolidinone with reduced toxicity for the treatment of tuberculosis
Crowley BM, Boshoff HI, Boving A, Tan VY, Zhu J, Hoyt F, Miller RR, Ehrhart J, Boyce CW, Young K, Nantermet PG, Su J, Yang L, Painter RE, Corcoran EB, Hoar JL, Oh S, Holtzman DL, Levi M, Anderson A, Otieno MA, Zimmerman M, Kaya F, Massoudi LM, Ramey ME, Bauman AA, Lenaerts AJ, Roberston GT, Dartois V, Wells CD, Barry CE 3rd, Olsen DB
Nat Med. 2026 Jan 13. doi: 10.1038/s41591-025-04164-x.

A multiomic framework for predicting laryngo-esophageal dysfunction following induction chemotherapy in hypopharyngeal-laryngeal carcinoma
Mattavelli D, Compagnoni M, Calza S, Ravanelli M, Plana M, Wichmann G, Paderno A, Zhan Y, Smussi D, Rondi P, Pinacoli A, Medda A, Lorini L, Khelik K, Hovig E, Maffini FA, Kalogerini M, Alberti A, Farina D, Dietz A, Mesia R, Piazza C, Chiocca S, Bossi P; PRESERVE Consortium
ESMO Open. 2025 Dec 29;11(1):105933. doi: 10.1016/j.esmoop.2025.105933.

Spatial proteomics of ovarian cancer precursors delineates early disease changes and drug targets
Makhmut A, Dragomir MP, Fritzsche S, Moebs M, Schmitt WD, Taube ET, and Coscia F
Mol Syst Biol, 13. November 2025

Neurosciences

Neural crest cell-derived DKK1 and NEDD4 modulate Wnt signalling in the second heart field to orchestrate outflow tract development
Wiszniak S, Alankarage D, Lohraseb I, Marchant C, Secker G, Domingo D, Hartmann J, Zhang T, Parker W, Toubia J, White M, Piltz S, Tondl M, Giannoulatou E, Winlaw D, Blue GM; Congenital Heart Disease Synergy Group; Tam PPL, Thomas P, Harvey N, Dunwoodie SL, Schwarz Q
Nat Commun. 2026 Jan 22. doi: 10.1038/s41467-026-68459-4.

IIIG9 and PP1α form a protein complex in the adherens junctions of polarized ependymal and MDCK cells
Oviedo MJ, Ramírez E, De Lima I, Grondona JM, Cifuentes M, Martínez F, Barahona MJ, Nualart F, Salazar K
Fluids Barriers CNS. 2026 Jan 21;23(1):12. doi: 10.1186/s12987-026-00762-0.

iPEX enables micrometre-resolution deep spatial proteomics via tissue expansion
Wang F, Sun C, Wu TW, Fu Y, Fan Y, Zhao S, Huang K, Pan Z, Lu Y, Han JR, Jia S, Zeng L, Zhang S, Chen T, An S, Meng SS, Guo X, Li W, Lian H, Sun X, Hu J, Yang C, Feng S, Li P, Du L, Liu X, Piatkevich KD, Zou Y
Nature. 2025 Nov 12. doi: 10.1038/s41586-025-09734-0.

Clinical/Pathology/Immunology Research Applications

Light Chain Cast Nephropathy: The Relationship between Tamm-Horsfall Protein Immunoreactivity and Clinical Pathological Features and Prognosis
Wang MY, Yong ZH, Wang S, Wang SX, Zhou XJ, Dong YJ, Zhao MH, Yu XJ, Zhou FD
Kidney Dis (2026)

Heterogeneous Nuclear Ribonucleoprotein A1 as a Key Regulator in Pulmonary Arterial Hypertension Development
Morimoto Y, Sato Y, Kato K, Yamamoto H, Suzuki K, Go K, Fukasawa Y, Ohashi N, Takahashi Y, Kato T
bioRxiv, Jan 9th 2026

Layer-specific proteomic analysis of human hearts in patients with sudden cardiac death
Kakimoto Y, Guan X, Ueda A, Kimura Y, Akiyama T, Tanaka M, Ikeda H, Maeda K, Isozaki S
PLoS One. 2025 Dec 30;20(12):e0339886. doi: 10.1371/journal.pone.0339886.

Developmental Research, Epigenetics & Aging

Comparative insights into molecular pathways influencing germline development in early-divergent angiosperms
Lora J, Tucker MR, Shirley NJ, Ma C, Hormaza JI
Plant J. 2026 Jan;125(2):e70675. doi: 10.1111/tpj.70675.

Genomic profiling of meiotic errors and early malignant transformation events in ovarian mature teratoma
Takahashi K, Tamura R, Suda K, Yachida N, Yamaguchi M, Mathew RM, Okuda S, Yoshihara K, Nakaoka H
Reproduction, xaaf019, Jan 15th 2026

Gene networks associated with early endosperm proliferation and basal endosperm layer differentiation in maize
Zhang S, Ran D, Ryu CH, Li G, Dannenhoffer JM, Wang X, Drews GN, and Yadegari R
bioRxiv, Jan 2nd 2026

Plant Research

Comparative insights into molecular pathways influencing germline development in early-divergent angiosperms
Lora J, Tucker MR, Shirley NJ, Ma C, Hormaza JI
Plant J. 2026 Jan;125(2):e70675. doi: 10.1111/tpj.70675.

Gene networks associated with early endosperm proliferation and basal endosperm layer differentiation in maize
Zhang S, Ran D, Ryu CH, Li G, Dannenhoffer JM, Wang X, Drews GN, and Yadegari R
bioRxiv, Jan 2nd 2026

Single-cell and spatial omics reveal progressive loss of xylem developmental complexity across seed plants
Shuai P, Hsieh JA, Kao CT, Hu CW, Wang R, Kuo SC, Yen MR, Liou PC, Ho YC, Chu CC, Huang S, Liu J, Zhang L, Wu CC, Luo YJ, Li Q, Hsu CC, Huang CL, Su JC, Tseng MC, Chen YL, Mai TL, Lin YJ
Plant Cell. 2025 Oct 19:koaf253. doi: 10.1093/plcell/koaf253.

Forensics

Invisible WItness: Innovations in the Collection of Touch DNA and the Risk of Forensic Corss Contamination
Irfan M, Haider SA, Yasir M, Akram MA, Adeel M
FORENSIC INSIGHTS AND HEALTH SCIENCES BULLETIN, Volume 3: Issue 2: 2025

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Miscellaneous

Expansion omics: from expansion microscopy to spatial omics
Dong Z, Xiang W, Jiang W, Guo T
Mol Syst Biol. 2025 Dec 1. doi: 10.1038/s44320-025-00171-9.

Similar growth potentials of cyanobacteria and algae explain their coexistence in desert soils
Kyi KM, Levintal E, A Kamennaya N
Sci Rep. 2025 Dec 1;16(1):830. doi: 10.1038/s41598-025-30489-1.

Morphology shapes microbial ecosystems and carbon cycling within cryoconite holes on a Greenland outlet glacier
Takeuchi N, Murakami T, Ishiwatari K, Watanabe A, Segawa T
Commun Earth Environ (2025)

Live Cell Culture

Muscle Structure and Function Recovery: Adalimumab-Calcium Channel Synergy in Post-Ischemic Stroke Sarcopenia
Qi H, Zhang XW, Zhang ZY, Ou SS, Gao YL, Tian D, Jiang YN, Min XR, Zhao AT, Zou JM, Zeng JS, Pu QY, Yang RC, Zeng N
J Cachexia Sarcopenia Muscle. 2025 Dec;16(6):e70097. doi: 10.1002/jcsm.70097.

Ultrasensitive proteomics uncovers nociceptor diversity and novel pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
bioRxiv, Sept 21, 2025

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

Laser microdissection (LMD) is an advanced, highly precise sample preparation technique that enables researchers to isolate pure, defined regions of interest, including single cells, from complex tissue samples. The method ensures reproducibility and accuracy for downstream analyses such as Next Generation Sequencing (NGS) including RNA-seq, and mass spectrometry for spatial genomics, spatial transcriptomics, spatial proteomics & spatial multiomics.

LMD allows non-contact dissection, isolation and gravity-assisted collection of targeted single cells, tissue compartments, cell clusters and even subcellular structures under visual control. These systems uniquely bridge microscopy and molecular biology to unlock true spatial omics insights. Combined with software-guided segmentation, Leica LMD systems enable high throughput workflows for biomarker discovery and identification of therapeutical interaction points. The study by Mund & Coscia et al., describing the Deep Visual Proteomics (DVP) workflow powered by the Leica LMD7 system, was featured on the cover of Nature Biotechnology in August 2022.  Follow-up research by Nordmann et al., published in Nature in 2024, demonstrated how DVP contributed to a life-saving therapy for toxic epidermal necrolysis (TEN), a deadly skin disease. These breakthroughs also contributed to the selection of “spatial proteomics” as Nature’s Method of the Year 2024.