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Das Wissensportal von Leica Microsystems bietet Ihnen Wissens- und Lehrmaterial zu den Themen der Mikroskopie. Die Inhalte sind so konzipiert, dass sie Einsteiger, erfahrene Praktiker und Wissenschaftler gleichermaßen bei ihrem alltäglichen Vorgehen und Experimenten unterstützen.

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Coherent Raman Scattering Microscopy Publication List

CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their molecules. The two most important CRS techniques are Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS). The biochemical image contrast of CRS is in many ways complementary to the molecular contrast obtained in fluorescence microscopy. A second crucial advantage of these methods is that they preserve the specimen/sample in a near pristine state. This reference list presents current and basic papers on CRS microscopy.

Clinical Microscopy: Considerations on Camera Selection

The need for images in pathology laboratories has significantly increased over the past few years, be it in histopathology, cytology, hematology, clinical microbiology, or other applications. They serve many purposes on top of the documentation of the diagnosis. Yet, the view through the eyepieces and the image are different in nature, the one is an optical image, the other a digital image. Looking at a few aspects of this process that are related cameras will help you make sure you can obtain the images with all the detail and color fidelity you need.

Factors to Consider when Selecting Clinical Microscopes

What matters if you would like to purchase a clinical microscope? Learn how to arrive at the best buying decision from our Science Lab Article.

Explore Alzheimer's Spatial Proteome with Big Data

Alzheimer's disease, a genetic and sporadic neurodegenerative condition, leads to cognitive decline in mid to late life, marked by β-amyloid plaques and tau tangles. With limited treatment options, new investigative strategies are crucial. The Cell DIVE multiplexed imaging solution allows examination of Alzheimer's brain tissue, potentially uncovering new research avenues. Here, we showcase the Cell DIVE image viewer, enabling users to access the full Alzheimer's multiplexed dataset directly in their browser.

Uncover the Hidden Complexity of Colon Cancer with Big Data

Colorectal cancer poses a significant health burden. While surgery is effective initially, some patients develop recurrent secondary disease with poor prognosis, necessitating advanced therapies like immunotherapies. Spatial biology approaches, such as multiplexed imaging with Cell DIVE, can provide crucial insights for developing novel treatments. Access the full Cell DIVE dataset in your browser to explore these findings further through the Minerva image viewer.

Dive into Pancreatic Cancer Research with Big Data

Pancreatic cancer, with a mortality rate near 40%, is challenging to treat due to its proximity to major organs. This story explores the complex biology of pancreatic ductal adenocarcinoma (PDAC), examining molecular and spatial determinants of tumor aggression in metabolism, apoptosis, and immunity. Access the full Cell DIVE dataset in your browser to delve deeper into these findings.

Overcoming Challenges with Microscopy when Imaging Moving Zebrafish Larvae

Zebrafish is a valuable model organism with many beneficial traits. However, imaging a full organism poses challenges as it is not stationary. Here, this case study shows how zebrafish larvae can be imaged during stationary periods and easily relocated after movement. The seamlessly integrated widefield and confocal capability of Mica is leveraged to capture fast events, like the heartbeat, with virtually no out-of-focus background noise which is inherent to standard widefield systems.

How to Automatically Obtain Fluorescent Cells of Interest in a Block-face

Block-face created by automatic trimming under fluorescence. Mammalian cells of interest, stained with CellTrackerTM Green are visualized within the block-face using the UC Enuity equipped with the stereo microscope M205 FA. In the background a carbon finder grid in black is visible.
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