Andreia Pinto , Dr.

Advanced Workflow Spezialistin, Leica Microsystems

Dr. Pinto begann ihre Karriere als biomedizinische Wissenschaftlerin in der histozellulären Pathologie mit besonderem Interesse an der Elektronenmikroskopie. Ab 2014 arbeitete sie bei Primary Ciliary Diagnosis (PCD) in Lissabon. Im Jahr 2019 wechselte Andreia an das Royal Brompton Hospital in London und arbeitete als Thoracic Research Associate, wo sie für das Trainieren einer Deep-Machine-Learning-Plattform zur Erkennung von Mustern in EM-Bildern von Zilien bei der Diagnose von PCD verantwortlich war. Zu diesem Thema schloss sie 2022 ihre Doktorarbeit ab und untersuchte gleichzeitig neue Erkenntnisse zur SARS-CoV-2-Infektion der Atemwege. Andreia ist derzeit Advanced Workflow Application Spezialistin bei Leica Microsystems am Imaging Center des European Molecular Biology Laboratory (EMBL) in Heidelberg.

Waffle method lamella preparation and cryo-ET results - Representative workflow and results of lamella preparation from a vitrified C. elegans L1 larva using the waffle method. Image courtesy: Prof. Dr. Jürgen M. Plitzko - doi.org/10.1038/s41592-023-02113-5-

Waffle Method Workflow: From HPF to Cryo-ET Lamellae

Waffle freezing provides an HPF-based route to cryo-ET sample preparation. This workflow guide follows the process from grid and carrier assembly to vitrification, cryo-FIB milling, lamella…

Complete camera overview of EM grid recorded with 3 channels. Inserts displaying the positions, where superresolved 3D confocal images were recorded. 3D renderings of these positions are shown in the zoomed inserts. Fluorescence channels (nuclei by Hoechst, blue; mitochondria by MitoTracker Green, green; lipid Droplets by Bodipy and Crimson Beads, red). Width of a grid square is 90 ?m, width of a grid bar is 35 ?m. Samples kindly provided by Ievgeniia Zagoriy, Mahamid-Group, EMBL Heidelberg, Germany.

From Bench to Beam: A Complete Correlative Cryo Light Microscopy Workflow

In the webinar entitled "A Multimodal Vitreous Crusade, a Cryo Correlative Workflow from Bench to Beam" a team of experts discusses the exciting world of correlative workflows for structural biology…