LMD6 & LMD7 Lasermikrodissektions-Systeme
Dissektion in Perfektion
This curated list features recent scientific publications that have employed Leica’s LMD6 and LMD7 laser microdissection systems in key areas such as cancer research, neuroscience, molecular pathology, forensics, and spatial omics. It is updated regularly to reflect the latest scientific publications utilizing laser microdissection applications.
Explore the full list below to see how Leica LMD systems are enabling scientific success across the globe.
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Single Cell Applications
Single-cell spatial proteomics maps human liver zonation patterns and their vulnerability to disruption in tissue architecture
Weiss CAM, Brown LA, Miranda L, Pellizzoni P, Steigerwald S, Remmert K, Hernandez JM, Borgwardt K, Kleiner DE, Ben-Moshe S, Rosenberger FA, Porat-Shliom N, Mann M
Nat Metab. 2026 Feb 20. doi: 10.1038/s42255-026-01459-2.
Laser Capture Microdissection: Methods and Protocols: Immunofluorescence Imaging-Guided Laser Microdissection for Ultralow Input Spatial Tissue Proteomics
Fritzsche S, Coscia F in Roberta Noberini (ed.)
Methods in Molecular Biology, vol. 3015
Separation and identification of two-individual mixed bloodstains using human neutrophil antigen-1 antibodies
Xia X, Zheng JL, Wang SW, Liu Z, Li Z, Wang DY, Li XN, Wu X, Yao J
Int J Legal Med. 2026 Jan 27. doi: 10.1007/s00414-026-03718-z.
Spatial Genomics (DNA-Reserach)
Identification of the Carcinogenic Process from Lobular Endocervical Glandular Hyperplasia to Gastric-Type Adenocarcinoma of the Uterine Cervix via Whole-Exome Sequencing
Kuruma A, Masuda T, Sato K, Kido K, Motooka D, Komura N, Yokoi T, Yoshihara K, Kinose Y, Hashimoto K, Sawada K, Morii E, Kimura T and Kodama M
Cancers2026, 18(4), 651; doi.org/10.3390/cancers18040651
Laser Capture Microdissection: Methods and Protocols: Laser Capture Microdissection from Blood Smears
Matsuo H in Roberta Noberini (ed.)
Methods in Molecular Biology, vol. 3015
Laser Microdissection and Near Single-Cell Whole Genome Amplification
Cruz-Flores R, Cáceres-Martínez J, Dhar AK
Methods Mol Biol. 2026;3003:165-173. doi: 10.1007/978-1-0716-5060-8_12.
Spatial Transcriptomics (RNA Research)
Dysregulation of estrogen signaling and HSD17B7 in the prostate stroma of African American men
Read GH, Pacheco JV, Baumann B, Richards Z, Deaton R, Macias V, Kajdacsy-Balla A, Kittles R, Abern MR, Nonn L
Endocrinology. 2026 Feb 17:bqag017. doi: 10.1210/endocr/bqag017.
A high-quality RNA-yielding protocol for laser capture microdissection of transplanted stem cell-derived Islets of Langerhans
Norman D, Lau J
Acta Diabetol. 2026 Feb 9. doi: 10.1007/s00592-026-02654-z.
Conjunctival allergic inflammation involving the interleukin-23/T helper type 17 immune axis in an experimental allergic conjunctivitis mouse model
Adachi R, Shoji J, Inada N, Tomioka A, Yamagami S
Jpn J Ophthalmol. 2026 Feb 13. doi: 10.1007/s10384-026-01333-4.
Proteomics, Lipidomics, Metabolomics
Spatial Profiling of Glycosaminoglycans (GAGomics) from Laser Microdissected Mouse Brain
Mernie E, Zaia J
Mol Cell Proteomics. 2026 Feb 19:101533. doi: 10.1016/j.mcpro.2026.101533.
Albumin/Keratin casts obstruct renal tubular and vascular lumens following kidney ischemia
Bryant M, Boykin O, Mosley O, Ajith A, Oglesby O, Kurzin K, Harris C, Husan S, Dokam D, McLarnon SR, Sun J, Marshall B, Barrett A, Polichnowski A, Bollag W, Mattson D, O’Connor PM
Kidney International Reports, 2026, 106356, ISSN 2468-0249
Spatial Proteomics Using S4P
Qin R, He F, Qin W
Bio-protocol 16(5): e5620. DOI: 10.21769/BioProtoc.5620.
Deep Visual Proteomics (DVP) related
Single-cell spatial proteomics maps human liver zonation patterns and their vulnerability to disruption in tissue architecture
Weiss CAM, Brown LA, Miranda L, Pellizzoni P, Steigerwald S, Remmert K, Hernandez JM, Borgwardt K, Kleiner DE, Ben-Moshe S, Rosenberger FA, Porat-Shliom N, Mann M
Nat Metab. 2026 Feb 20. doi: 10.1038/s42255-026-01459-2.
Spatial protein gradients pattern liver metabolism
Dong Z, Guo T
Nat Metab (2026). doi.org/10.1038/s42255-026-01478-z
Laser Capture Microdissection: Methods and Protocols: Immunofluorescence Imaging-Guided Laser Microdissection for Ultralow Input Spatial Tissue Proteomics
Fritzsche S, Coscia F in Roberta Noberini (ed.)
Methods in Molecular Biology, vol. 3015
Fluorescence Applications
Laser Capture Microdissection: Methods and Protocols: A Spatial Proteomics Approach to Isolate and Analyze Amyloid Proteins Using Laser Capture Microdissection and Mass Spectrometry
Aguilan J, Horton M, Pessin JE, and Sidoli S in Roberta Noberini (ed.)
Methods in Molecular Biology, vol. 3015
Separation and identification of two-individual mixed bloodstains using human neutrophil antigen-1 antibodies
Xia X, Zheng JL, Wang SW, Liu Z, Li Z, Wang DY, Li XN, Wu X, Yao J
Int J Legal Med. 2026 Jan 27. doi: 10.1007/s00414-026-03718-z.
Congophilic fibrillary glomerulonephritis recurrence post-renal transplant: diagnostic challenges and proteomic insights
Watanabe H, Nagamine M, Shintani-Domoto Y, Kawanishi K, Ishino K, Nakamura T, Sumiyoshi S, Konishi E
CEN Case Rep. 2026 Jan 23;15(1):34. doi: 10.1007/s13730-025-01064-4.
Cancer Research
Identification of the Carcinogenic Process from Lobular Endocervical Glandular Hyperplasia to Gastric-Type Adenocarcinoma of the Uterine Cervix via Whole-Exome Sequencing
Kuruma A, Masuda T, Sato K, Kido K, Motooka D, Komura N, Yokoi T, Yoshihara K, Kinose Y, Hashimoto K, Sawada K, Morii E, Kimura T and Kodama M
Cancers2026, 18(4), 651; doi.org/10.3390/cancers18040651
Dysregulation of estrogen signaling and HSD17B7 in the prostate stroma of African American men
Read GH, Pacheco JV, Baumann B, Richards Z, Deaton R, Macias V, Kajdacsy-Balla A, Kittles R, Abern MR, Nonn L
Endocrinology. 2026 Feb 17:bqag017. doi: 10.1210/endocr/bqag017.
Oncogenic Signaling Activation and Potential Biomarkers in Congestive Hepatopathy Revealed by Proteomic Analysis
Morimoto Y, Go K, Suzuki K, Yamamoto H, Fukasawa Y, Ohashi N, Takahashi Y, Kato T
Hepatol Res. 2026 Jan 29. doi: 10.1111/hepr.70133.
Neurosciences
Human brain prefrontal cortex proteomics identifies compromised energy metabolism and neuronal function in Schizophrenia
Koopmans F, Dijkstra AA, Li WP, Klaassen RV, Gouwenberg Y, Yao S, Bast L, Verhage M, Karlsson R, Dwork AJ, Stockmeier CA, Hjerling-Leffler J, Sullivan PF, Smit AB
Nat Commun. 2026 Jan 29. doi: 10.1038/s41467-026-68950-y.
Proteomic remodelling of the neurobrillary tangle from “PART” to advanced Alzheimer’s disease
Thierry M, Kavanagh T, Balcomb K, Tang L, Leitner D, Kanshin E, William C, Oakley D, Hyman B, Ueberheide B, Drummond E, Wisniewski T
Research Square, 29th Jan 2026
Neural crest cell-derived DKK1 and NEDD4 modulate Wnt signalling in the second heart field to orchestrate outflow tract development
Wiszniak S, Alankarage D, Lohraseb I, Marchant C, Secker G, Domingo D, Hartmann J, Zhang T, Parker W, Toubia J, White M, Piltz S, Tondl M, Giannoulatou E, Winlaw D, Blue GM; Congenital Heart Disease Synergy Group; Tam PPL, Thomas P, Harvey N, Dunwoodie SL, Schwarz Q
Nat Commun. 2026 Jan 22. doi: 10.1038/s41467-026-68459-4.
Clinical/Pathology/Immunology Research Applications
Single-cell spatial proteomics maps human liver zonation patterns and their vulnerability to disruption in tissue architecture
Weiss CAM, Brown LA, Miranda L, Pellizzoni P, Steigerwald S, Remmert K, Hernandez JM, Borgwardt K, Kleiner DE, Ben-Moshe S, Rosenberger FA, Porat-Shliom N, Mann M
Nat Metab. 2026 Feb 20. doi: 10.1038/s42255-026-01459-2.
Conjunctival allergic inflammation involving the interleukin-23/T helper type 17 immune axis in an experimental allergic conjunctivitis mouse model
Adachi R, Shoji J, Inada N, Tomioka A, Yamagami S
Jpn J Ophthalmol. 2026 Feb 13. doi: 10.1007/s10384-026-01333-4.
Albumin/Keratin casts obstruct renal tubular and vascular lumens following kidney ischemia
Bryant M, Boykin O, Mosley O, Ajith A, Oglesby O, Kurzin K, Harris C, Husan S, Dokam D, McLarnon SR, Sun J, Marshall B, Barrett A, Polichnowski A, Bollag W, Mattson D, O’Connor PM
Kidney International Reports, 2026, 106356, ISSN 2468-0249
NAD boosting mediated by CD38 inhibition drives reversal of a pathological vicious cycle of intracrine activity and inflammation in eyelid meibomian gland dysfunction
Hamada YSakamoto, T, Yarimizu D, Uehara H, Shao X, Macpherson T, Hasegawa E, Doi M
bioRxiv, 10 Feb 2026
Developmental Research, Epigenetics & Aging
A high-quality RNA-yielding protocol for laser capture microdissection of transplanted stem cell-derived Islets of Langerhans
Norman D, Lau J
Acta Diabetol. 2026 Feb 9. doi: 10.1007/s00592-026-02654-z.
Comparative insights into molecular pathways influencing germline development in early-divergent angiosperms
Lora J, Tucker MR, Shirley NJ, Ma C, Hormaza JI
Plant J. 2026 Jan;125(2):e70675. doi: 10.1111/tpj.70675.
Genomic profiling of meiotic errors and early malignant transformation events in ovarian mature teratoma
Takahashi K, Tamura R, Suda K, Yachida N, Yamaguchi M, Mathew RM, Okuda S, Yoshihara K, Nakaoka H
Reproduction, xaaf019, Jan 15th 2026
Plant Research
Comparative insights into molecular pathways influencing germline development in early-divergent angiosperms
Lora J, Tucker MR, Shirley NJ, Ma C, Hormaza JI
Plant J. 2026 Jan;125(2):e70675. doi: 10.1111/tpj.70675.
Gene networks associated with early endosperm proliferation and basal endosperm layer differentiation in maize
Zhang S, Ran D, Ryu CH, Li G, Dannenhoffer JM, Wang X, Drews GN, and Yadegari R
bioRxiv, Jan 2nd 2026
Single-cell and spatial omics reveal progressive loss of xylem developmental complexity across seed plants
Shuai P, Hsieh JA, Kao CT, Hu CW, Wang R, Kuo SC, Yen MR, Liou PC, Ho YC, Chu CC, Huang S, Liu J, Zhang L, Wu CC, Luo YJ, Li Q, Hsu CC, Huang CL, Su JC, Tseng MC, Chen YL, Mai TL, Lin YJ
Plant Cell. 2025 Oct 19:koaf253. doi: 10.1093/plcell/koaf253.
Forensics
Separation and identification of two-individual mixed bloodstains using human neutrophil antigen-1 antibodies
Xia X, Zheng JL, Wang SW, Liu Z, Li Z, Wang DY, Li XN, Wu X, Yao J
Int J Legal Med. 2026 Jan 27. doi: 10.1007/s00414-026-03718-z.
Invisible WItness: Innovations in the Collection of Touch DNA and the Risk of Forensic Corss Contamination
Irfan M, Haider SA, Yasir M, Akram MA, Adeel M
FORENSIC INSIGHTS AND HEALTH SCIENCES BULLETIN, Volume 3: Issue 2: 2025
Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.
Miscellaneous
Spatial Profiling of Glycosaminoglycans (GAGomics) from Laser Microdissected Mouse Brain
Mernie E, Zaia J
Mol Cell Proteomics. 2026 Feb 19:101533. doi: 10.1016/j.mcpro.2026.101533.
Spatial Proteomics Using S4P
Qin R, He F, Qin
Bio-protocol 16(5): e5620. DOI: 10.21769/BioProtoc.5620.
Structural and chemical compositional characteristics of rubberwood with gelatinous fibre distribution in the tension and opposite wood
Tu X, Li T, Li J, Zhang F, Liu H & Yang L
Wood Sci Technol 60, 28 (2026).
Live Cell Culture
Muscle Structure and Function Recovery: Adalimumab-Calcium Channel Synergy in Post-Ischemic Stroke Sarcopenia
Qi H, Zhang XW, Zhang ZY, Ou SS, Gao YL, Tian D, Jiang YN, Min XR, Zhao AT, Zou JM, Zeng JS, Pu QY, Yang RC, Zeng N
J Cachexia Sarcopenia Muscle. 2025 Dec;16(6):e70097. doi: 10.1002/jcsm.70097.
Ultrasensitive proteomics uncovers nociceptor diversity and novel pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
bioRxiv, Sept 21, 2025
A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX
Laser microdissection (LMD) is an advanced, highly precise sample preparation technique that enables researchers to isolate pure, defined regions of interest, including single cells, from complex tissue samples. The method ensures reproducibility and accuracy for downstream analyses such as Next Generation Sequencing (NGS) including RNA-seq, and mass spectrometry for spatial genomics, spatial transcriptomics, spatial proteomics & spatial multiomics.
LMD allows non-contact dissection, isolation and gravity-assisted collection of targeted single cells, tissue compartments, cell clusters and even subcellular structures under visual control. These systems uniquely bridge microscopy and molecular biology to unlock true spatial omics insights. Combined with software-guided segmentation, Leica LMD systems enable high throughput workflows for biomarker discovery and identification of therapeutical interaction points. The study by Mund & Coscia et al., describing the Deep Visual Proteomics (DVP) workflow powered by the Leica LMD7 system, was featured on the cover of Nature Biotechnology in August 2022. Follow-up research by Nordmann et al., published in Nature in 2024, demonstrated how DVP contributed to a life-saving therapy for toxic epidermal necrolysis (TEN), a deadly skin disease. These breakthroughs also contributed to the selection of “spatial proteomics” as Nature’s Method of the Year 2024.
