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Leica LMD6 & LMD7 Lasermikrodissektionssysteme

Dissektion in Perfektion

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Now there is single-cell Deep Visual Proteomics (scDVP), a technology that integrates high-content imaging, laser microdissection, and multiplexed mass spectrometry (MS) for tissue characterization at the single cell level.

Download the complete list of LMD publications in "Downloads"

DNA Research

Efficient cancer modeling through CRISPR-Cas9/HDR-based somatic precision gene editing in mice
Bu W, Creighton CJ, Heavener KS, Gutierrez C, Dou Y, Ku AT, Zhang Y, Jiang W, Urrutia J, Jiang W, Yue F, Jia L, Ibrahim AA, Zhang B, Huang S, Li Y
Sci Adv. 2023 May 12;9(19):eade0059. doi: 10.1126/sciadv.ade0059.

Development of a Novel Method for the Clinical Visualization and Rapid Identification of Multidrug-Resistant Candida auris
Zhang XR, Ma T, Wang YC, Hu S, Yang Y
Microbiol Spectr. 2023 Apr 26:e0491222. doi: 10.1128/spectrum.04912-22.

Clinicopathological features and genetic alterations in mixed-type ampullary carcinoma
Kawata J, Koga Y, Noguchi S, Shimada Y, Yamada Y, Yamamoto T, Shindo K, Nakamura M, Oda Y
Mod Pathol. 2023 Mar 31:100181. doi: 10.1016/j.modpat.2023.100181.

RNA Research

Basal forebrain cholinergic neurons are vulnerable in a mouse model of Down syndrome and their molecular fingerprint is rescued by maternal choline supplementation
Alldred MJ, Pidikiti H, Heguy A, Roussos P, Ginsberg SD
FASEB J. 2023 Jun;37(6):e22944. doi: 10.1096/fj.202202111RR.

Spatiotemporal profiles of gene activity in stamen delineate nucleo-cytoplasmic interaction in a male sterile somatic cybrid citrus
Jiang N, Feng MQ, Cheng LC, Kuang LH, Li CC, Ying ZP, Wang R, Xie KD, Guo WW, Wu XM
Horticulture Research, uhad105,

Tau pathology epigenetically remodels the neuron-glial cross-talk in Alzheimer's disease
Zhou LT, Liu D, Kang HC, Lu L, Huang HZ, Ai WQ, Zhou Y, Deng MF, Li H, Liu ZQ, Zhang WF, Hu YZ, Han ZT, Zhang HH, Jia JJ, Sarkar AK, Sharaydeh S, Wang J, Man HY, Schilling M, Bertram L, Lu Y, Guo Z, Zhu LQ
Sci Adv. 2023 Apr 21;9(16):eabq7105. doi: 10.1126/sciadv.abq7105.

Proteomics, Lipidomics, Metabolomics

Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation
Wang Z, Zhang Z, Li Y, Zhang Y, Wei M, Li H, Yang S, Zhou Y, Zhou X, Xing G
Hypertens Res. 2023 May 15:1-12. doi: 10.1038/s41440-023-01300-3.

Immobility-associated thromboprotection is conserved across mammalian species from bear to human
Thienel M, Müller-Reif JB, Zhang Z, Ehreiser V, Huth J, Shchurovska K, Kilani B, Schweizer L, Geyer PE, Zwiebel M, Novotny J, Lüsebrink E, Little G, Orban M, Nicolai L, El Nemr S, Titova A, Spannagl M, Kindberg J, Evans AL, Mach O, Vogel M, Tiedt S, Ormanns S, Kessler B, Dueck A, Friebe A, Jørgensen PG, Majzoub-Altweck M, Blutke A, Polzin A, Stark K, Kääb S, Maier D, Gibbins JM, Limper U, Frobert O, Mann M, Massberg S, Petzold T
Science. 2023 Apr 14;380(6641):178-187. doi: 10.1126/science.abo5044.

Identification of Uromodulin Deposition in the Stroma of Perinephric Fibromyxoid Nephrogenic Adenoma by Mass Spectrometry
Yoshimura K, Ito Y, Suzuki M, Horie M, Nishiuchi T, Shintani-Domoto Y, Shigehara K, Ohshima H, Ohshima M, Goto A, Nojima T, Tsuzuki T, Mizokami A, Ikeda H, Maeda D
Reasearcg Square, 12th April 2023

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
Mund A, Coscia F, Kriston A, Hollandi R, Kovacs F, Brunner A-D, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
Nat Biotechnol (2022). doi.org/10.1038/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8
Now even on single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1101/2022.12.03.518957

Fluorescence Applications

miR-34a regulates macrophage-associated inflammation and angiogenesis in alcohol-induced liver injury
Wan Y, Slevin E, Koyama S, Huang CK, Shetty AK, Li X, Harrison K, Li T, Zhou B, Lorenzo SR, Zhang Y, Salinas JM, Xu W, Klaunig JE, Wu C, Tsukamoto H, Meng F
Hepatol Commun. 2023 Apr 4;7(4):e0089. doi: 10.1097/HC9.0000000000000089.

Renal function in aged C57BL/6J mice is impaired by deposition of age-related apolipoprotein A-II amyloid independent of kidney aging
Li Y, Dai J, Kametani F, Yazaki M, Ishigami A, Mori M, Miyahara H, Higuchi K
Am J Pathol. 2023 Mar 23:S0002-9440(23)00112-8. doi: 10.1016/j.ajpath.2023.03.002.

B cell-derived anti-beta 2 glycoprotein I antibody mediates hyperhomocysteinemia-aggravated hypertensive glomerular lesions by triggering ferroptosis
Du X, Ma X, Tan Y, Shao F, Li C, Zhao Y, Miao Y, Han L, Dang G, Song Y, Yang D, Deng Z, Wang Y, Jiang C, Kong W, Feng J, Wang X
Signal Transduct Target Ther. 2023 Mar 13;8(1):103. doi: 10.1038/s41392-023-01313-x.

Cancer Research

Label-Free Quantification Mass Spectrometry Identifies Protein Markers of Chemotherapy Response in High-Grade Serous Ovarian Cancer
Arentz G, Mittal P, Klingler-Hoffmann M, Condina MR, Ricciardelli C, Lokman NA, Kaur G, Oehler MK, and Hoffmann P
Cancers 2023, 15(7), 2172

Neurosciences

Localization and Diagnostic Specificity of Glutamic Acid Decarboxylase Transcript Alterations in the Dorsolateral Prefrontal Cortex in Schizophrenia
Dowling KF, Dienel SJ, Barile Z, Bazmi HH, Lewis DA
Biol Psychiatry. 2023 Apr 13:S0006-3223(23)01199-X. doi: 10.1016/j.biopsych.2023.04.003.

Clinical/Pathology/Immunology Research Applications

Developmental Research, Epigenetics & Aging

Gene editing to prevent ventricular arrhythmias associated with cardiomyocyte cell therapy
Marchiano S, Nakamura K, Reinecke H, Neidig L, Lai M, Kadota S, Perbellini F, Yang X, Klaiman JM, Blakely LP, Karbassi E, Fields PA, Fenix AM, Beussman KM, Jayabalu A, Kalucki FA, Futakuchi-Tsuchida A, Weber GJ, Dupras S, Tsuchida H, Pabon L, Wang L, Knollmann BC, Kattman S, Thies RS, Sniadecki N, MacLellan WR, Bertero A, Murry CE
Cell Stem Cell. 2023 Apr 6;30(4):396-414.e9. doi: 10.1016/j.stem.2023.03.010.

Targeted epigenetic silencing of UCHL1 expression suppresses collagen-1 production in human lung epithelial cells
Wu D-D, Lau ATY, Xu Y-M, Reinders-Luinge M, Koncz M, Kiss A, Timens W, Rots MG, and Hylkema MN
Epigenetics 2023, VOL. 18, NO. 1, 2175522

Overview of single-cell RNA sequencing analysis and its application to spermatogenesis research
Suzuki T
Reprod Med Biol. 2023;22:e12502.

Plant Research

Genomics of Crucifer’s Host-Pathosystem
Saharan GS, Mehta NK, Meena PD
Bookchapter, Springer Nature Singapore 2023.

The RLCK-VND6 module coordinates secondary cell wall formation and adaptive growth in rice
Cao S, Wang Y, Gao Y, Xu R, Ma J, Xu Z, Shang-Guan K, Zhang B, Zhou Y
Mol Plant. 2023 Apr 12:S1674-2052(23)00104-1. doi: 10.1016/j.molp.2023.04.006.

Forensics

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
Sun M, Zheng J, Wnag B and Yao J:
BioMed Research International, Volume 2021 | Article ID 7269237

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Further information on Leica LMD impact at forensics:
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Morin exhibits a neuroprotective effect in MPTP-induced Parkinson's disease model via TFEB/AMPK-mediated mitophagy
Wang Z, Cui J, Li D, Ran S, Huang J, Chen G
Phytomedicine. 2023 May 13;116:154866. doi: 10.1016/j.phymed.2023.154866.

Toxoplasma gondii HOOK-FTS-HIP Complex is Critical for Secretory Organelle Discharge during Motility, Invasion, and Egress
Dubois DJ, Chehade S, Marq JB, Venugopal K, Maco B, Puig ATI, Soldati-Favre D, Marion S
mBio. 2023 Apr 24:e0045823. doi: 10.1128/mbio.00458-23.

MALDImID: Spatialomics R package and Shiny app for more specific identification of MALDI imaging proteolytic peaks using LC-MS/MS-based proteomic biomarker discovery data
Oliveira C, Longuespée R
Proteomics – Proteomics and Systems Biology, 3rd April 2023, DOI:10.1002/pmic.202300005

Live Cell Culture

An efficient semi–in vivo zygotic embryogenesis system in Arabidopsis
Li J, Li SS, Liu Y, Tang WY, Liu XF, Meng CF, Zhang LY, Sun MX
In Vitro Cell.Dev.Biol.-Plant, 27. Dec 2022

Direct Reprogramming of Mouse Subchondral Bone Osteoblasts into Chondrocyte-Like Cells
Li M, Zhang L, Li J, and Zhu Q
Biomedicines 2022, 10, 2582.

Heterotypic cell-in-cell structures between cancer and NK cells are associated with enhanced anticancer drug resistance
Choe YJ, Min JY, Lee H, Lee SY, Kwon J, Kim HJ, Lee J, Kim HM, Park HS, Cho MY, Hyun JY, Kim HM, Chung YH, Ha SK, Jeong HG, Choi I, Kim TD, Hong KS, and Han EH
iScience 25, 105017 September 16, 2022