LMD6 & LMD7 Lasermikrodissektions-Systeme

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

Analysis of microisolated frontal cortex excitatory layer III and V pyramidal neurons reveals a neurodegenerative phenotype in individuals with Down syndrome
Alldred MJ, Pidikiti H, Ibrahim KW, Lee SH, Heguy A, Hoffman GE, Roussos P, Wisniewski T, Wegiel J, Stutzmann GE, Mufson EJ, Ginsberg SD
Acta Neuropathol. 2024 Aug 6;148(1):16. doi: 10.1007/s00401-024-02768-0.

Deciphering functional tumor–immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann M
bioRxiv preprint, May 23, 2024

The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma
Hu H, Liu G, Li Y
Plant Methods. 2024 May 17;20(1):72. doi: 10.1186/s13007-024-01184-5.

DNA Research

Origin of Peritoneal Cancer With Features of High-grade Serous Carcinoma: A Detailed Molecular Analysis
Yamagata T, Watanabe K, Yamanoi K, Kakiuchi N, Ogura J, Taki M, Murakami R, Yamaguchi K, Hamanishi J, Minamiguchi S, Ogawa S, Mandai M
Int J Gynecol Pathol. 2024 Sep 10. doi: 10.1097/PGP.0000000000001069.

Unraveling the key role of chromatin structure in cancer development through epigenetic landscape characterization of oral cancer
Xue Y, Liu L, Zhang Y, He Y, Wang J, Ma Z, Li TJ, Zhang J, Huang Y, Gao YQ
Mol Cancer. 2024 Sep 6;23(1):190. doi: 10.1186/s12943-024-02100-0.

Differences in BRAF V600E mutation between the epithelium and mesenchyme in classic ameloblastoma
Chen Z, Hong Y, Zhao Z, Wu N, Ma X, Chen L, Zhang R 
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 21 Aug 2024

RNA Research

Histological differences related to autophagy in the minor salivary gland between primary and secondary types of Sjögren's syndrome
Ono-Minagi H, Nohno T, Takabatake K, Tanaka T, Katsuyama T, Miyawaki K, Wada J, Ibaragi S, Iida S, Yoshino T, Nagatsuka H, Sakai T, Ohuchi H
BMC Oral Health. 2024 Sep 16;24(1):1099. doi: 10.1186/s12903-024-04869-4.

Aberrant localization of β1 integrin in podocyte cytoplasm of primary FSGS with cellular lesion
Katafuchi E, Hisano S, Kurata S, Muta K, Uesugi N, Miyamoto T, Harada Y, Shimajiri S, Katafuchi R, Nakayama T
Virchows Arch. 2024 Sep 13. doi: 10.1007/s00428-024-03919-0.

Collagen-Heparin-FGF2-VEGF Scaffolds Induce a Regenerative Gene Expression Profile in a Fetal Sheep Wound Model
Gansevoort M, Oostendorp C, Bouwman LF, Tiemessen DM, Geutjes PJ, Feitz WFJ, van Kuppevelt TH, Daamen WF
Tissue Eng Regen Med. 2024 Aug 31. doi: 10.1007/s13770-024-00667-9.

Proteomics, Lipidomics, Metabolomics

Proteomics based selection achieves complete response to HER2 therapy in HER2 IHC 0 breast cancer
Johnston LE, Randall J, Chouraichi S, Luu M, Hunt AL, Mauro L, Mueller C, Davis JB, Petricoin EF, Conrads TP, Cannon TL, Huynh J
NPJ Precis Oncol. 2024 Sep 14;8(1):203. doi: 10.1038/s41698-024-00696-6.

Dose optimization of TBI-223 for enhanced therapeutic benefit compared to linezolid in antituberculosis regimen
Strydom N, Ernest JP, Imperial M, Solans BP, Wang Q, Tasneen R, Tyagi S, Soni H, Garcia A, Bigelow K, Gengenbacher M, Zimmerman M, Xie M, Sarathy JP, Yang TJ, Dartois V, Nuermberger EL, Savic RM
Nat Commun. 2024 Aug 25;15(1):7311. doi: 10.1038/s41467-024-50781-4.

Proteomic analysis of laser captured tubular tissues reveals complement activation and mitochondrial dysfunction in autoimmune related kidney diseases
Xiao M, Chi X, Zhu X, Xu Z, Zou Y, Peng Y, Luan S, Dong J, Dai Y, Yin L
Sci Rep. 2024 Aug 20;14(1):19311. doi: 10.1038/s41598-024-70209-9.

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8
Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Fluorescence Applications

Deciphering functional tumor–immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann M
bioRxiv preprint, May 23, 2024

The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma
Hu H, Liu G, Li Y
Plant Methods. 2024 May 17;20(1):72. doi: 10.1186/s13007-024-01184-5.

Deep Visual Proteomics advances human colon organoid models by revealing a switch to an in vivo-like phenotype upon xenotransplantation
Post F, Hausmann A, Kabatnik S, Steigerwald S, Brand A, Clement DL, Skov J, Boyce TL, Sato T, Steenholdt C, Mund A, Nielsen OH, Jensen KB, Mann M
bioRxiv preprint, doi: doi.org/10.1101/2024.05.13.593888; May 14, 2024

Cancer Research

Integrated multi-omics assessment of lineage plasticity in a prostate cancer patient with brain and dural metastases
Ludwig ML, Moline D, Horrmann A, Boytim E, Larson G, Arafa AT, Sayeda M, Lozada JR, Bergom HE, Day A, Dasaraju S, Dehm SM, Murugan P, Hwang J, Drake JM, Antonarakis ES
NPJ Precis Oncol. 2024 Sep 30;8(1):215. doi: 10.1038/s41698-024-00713-8.

Proteomics based selection achieves complete response to HER2 therapy in HER2 IHC 0 breast cancer
Johnston LE, Randall J, Chouraichi S, Luu M, Hunt AL, Mauro L, Mueller C, Davis JB, Petricoin EF, Conrads TP, Cannon TL, Huynh J
NPJ Precis Oncol. 2024 Sep 14;8(1):203. doi: 10.1038/s41698-024-00696-6.

Unraveling the key role of chromatin structure in cancer development through epigenetic landscape characterization of oral cancer
Xue Y, Liu L, Zhang Y, He Y, Wang J, Ma Z, Li TJ, Zhang J, Huang Y, Gao YQ
Mol Cancer. 2024 Sep 6;23(1):190. doi: 10.1186/s12943-024-02100-0.

Neurosciences

rTMS mechanisms for posttraumatic stress disorder treatment in a mouse model
Claverie D, Cressant A, Thomasson J, Castellarin C, Grandperret V, Barbier L, Troubat R, Canini F, Belzung C, El-Hage W
Journal of Psychiatric Research, 28 Aug 2024, S0022-3956(24)00506-5

Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues
Watanabe K, Takayama S, Yamada T, Hashimoto M, Tadano J, Nakagawa T, Watanabe T, Fukusaki E, Miyawaki I, Shimma S
Anal Bioanal Chem. 2024 Aug 10. doi: 10.1007/s00216-024-05477-5.

Analysis of microisolated frontal cortex excitatory layer III and V pyramidal neurons reveals a neurodegenerative phenotype in individuals with Down syndrome
Alldred MJ, Pidikiti H, Ibrahim KW, Lee SH, Heguy A, Hoffman GE, Roussos P, Wisniewski T, Wegiel J, Stutzmann GE, Mufson EJ, Ginsberg SD
Acta Neuropathol. 2024 Aug 6;148(1):16. doi: 10.1007/s00401-024-02768-0.

Clinical/Pathology/Immunology Research Applications

Histological differences related to autophagy in the minor salivary gland between primary and secondary types of Sjögren's syndrome
Ono-Minagi H, Nohno T, Takabatake K, Tanaka T, Katsuyama T, Miyawaki K, Wada J, Ibaragi S, Iida S, Yoshino T, Nagatsuka H, Sakai T, Ohuchi H
BMC Oral Health. 2024 Sep 16;24(1):1099. doi: 10.1186/s12903-024-04869-4.

Aberrant localization of β1 integrin in podocyte cytoplasm of primary FSGS with cellular lesion
Katafuchi E, Hisano S, Kurata S, Muta K, Uesugi N, Miyamoto T, Harada Y, Shimajiri S, Katafuchi R, Nakayama T
Virchows Arch. 2024 Sep 13. doi: 10.1007/s00428-024-03919-0.

A reliable clinical test for detection of membranous nephropathyantigens using laser microdissection and mass spectrometry
Vrana JA, Theis JD, Wegwerth PJ, Dasari S, Madden B, Nasr SH, Fidler M,McPhail ED, Fervenza FC, Sethi S
Kidney International (2024), doi: doi.org/10.1016/j.kint.2024.07.031

Developmental Research, Epigenetics  & Aging

Unraveling the key role of chromatin structure in cancer development through epigenetic landscape characterization of oral cancer
Xue Y, Liu L, Zhang Y, He Y, Wang J, Ma Z, Li TJ, Zhang J, Huang Y, Gao YQ
Mol Cancer. 2024 Sep 6;23(1):190. doi: 10.1186/s12943-024-02100-0.

Collagen-Heparin-FGF2-VEGF Scaffolds Induce a Regenerative Gene Expression Profile in a Fetal Sheep Wound Model
Gansevoort M, Oostendorp C, Bouwman LF, Tiemessen DM, Geutjes PJ, Feitz WFJ, van Kuppevelt TH, Daamen WF
Tissue Eng Regen Med. 2024 Aug 31. doi: 10.1007/s13770-024-00667-9.

Molecular basis of urostyle development in frogs: genes and gene regulation underlying an evolutionary novelty
Senevirathne G, Shubin NH
Open Biol. 2024 Aug;14(8):240111. doi: 10.1098/rsob.240111.

Plant Research

Transport and spatio-temporal conversion of sugar facilitate the formation of spatial gradients of starch in wheat caryopse
Zhong Y, Chen Y, Pan M, Li X, Hebelstrup K, Cai J, Zhou Q, Dai T, Cao W, Jiang D
Commun Biol. 2024 Aug 2;7(1):928. doi: 10.1038/s42003-024-06625-4.

Developing a simple and rapid method for cell-specific transcriptome analysis through laser microdissection: insights from citrus rind with broader implications
Mei Z, Zhu K, Yen D, Jia H, Luo W, Ye J, Deng X
Plant Methods. 2024 Jul 27;20(1):113. doi: 10.1186/s13007-024-01242-y.

Soybean symbiotic-nodule zonation and cell differentiation are defined by NIN2 signaling and GH3-dependent auxin homeostasis
Tu T, Gao Z, Li L, Chen J, Ye K, Xu T, Mai S, Han Q, Chen C, Wu S, Dong Y, Chen J, Huang L, Guan Y, Xie F, Chen X
Dev Cell. 2024 Jul 17:S1534-5807(24)00424-6. doi: 10.1016/j.devcel.2024.07.001.

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Miscellaneous

The Oxylipin Dependent Quorum Sensing System enhances Pseudomonas aeruginosa dissemination during burn-associated infection
Martinez E, Im H, Campos-Gomez J, Orihuela CJ
Res Square, preprint, Version 1, posted 25 Sep, 2024

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

Cytokinetic abscission in Toxoplasma gondii is governed by protein phosphatase 2A and the daughter cell scaffold complex
Marq JB, Gosetto M, Altenried A, Vadas O, Maco B, Dos Santos Pacheco N, Tosetti N, Soldati-Favre D, Lentini G
EMBO J. 2024 Jul 15. doi: 10.1038/s44318-024-00171-9.

Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23

Live Cell Culture

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416