Leica Microsystems

Leica Microsystems

Leica Microsystems ist ein weltweit führender Hersteller von Mikroskopen und wissenschaftlichen Instrumenten. Im 19. Jahrhundert als Familienunternehmen gegründet, war die Geschichte des Unternehmens auf dem Weg zum Weltkonzern von beispielloser Innovation geprägt.

Die traditionell enge Zusammenarbeit mit der Wissenschaft ist der Schlüssel zur Innovationstradition von Leica Microsystems, die auf die Ideen der Anwender zurückgreift und auf deren Bedürfnisse zugeschnittene Lösungen schafft. Auf globaler Ebene ist Leica Microsystems in drei Divisionen gegliedert, die alle zu den führenden Anbietern in ihrem jeweiligen Bereich gehören: Life Science, Industrie und Medizin.

Das Unternehmen ist in über 100 Ländern mit 6 Produktionsstandorten in 5 Ländern, Vertriebs- und Serviceorganisationen in 20 Ländern und einem internationalen Händlernetz vertreten. Der Hauptsitz des Unternehmens liegt in Wetzlar, Deutschland.

https://www.leica-microsystems.com/de

UC Enuity with Diatome diamond knife.

Mastering Polymer Sectioning with Helmut Gnaegi

When it comes to ultramicrotomy, few names carry the weight of Helmut Gnaegi. As co-founder of Diatome, a global leader in diamond knife technology, Helmut has spent decades refining the art and…

Zebrafisch-Forschung

Für optimale Ergebnisse während der Bewertung, Sortierung, Manipulation und Bildgebung von Modellorganismen ist es entscheidend feine Details und Strukturen genauestens zu erkennen. Das bildet die…
3D high-plex imaging in cancer immunology. Overview of a pancreatic tumor section in mouse model, labeled with 15 markers and imaged in one go using STELLARIS with SpectraPlex. (https://www.nature.com/articles/d42473-024-00260-7)

How to Streamline High-Plex Imaging for 3D Spatial Omics Advances

In this webinar, Dr. Julia Roberti and Dr. Luis Alvarez from Leica Microsystems introduce SpectraPlex, a new functionality integrated into the STELLARIS confocal platform for high-plex 3D spatial…
Evolved ARveo and MyVeo in Operating Room

The Guide to Augmented Reality in Microsurgery

In an era of technological advancement, Augmented Reality (AR) is rapidly transforming the medical field. In surgical microscopy, AR can display fluorescence signals as digital overlays in real-time…
Zebrafish-embryo image captured using a THUNDER Imager Tissue and live instant computational clearing.

Improving Zebrafish-Embryo Screening with Fast, High-Contrast Imaging

Discover from this article how screening of transgenic zebrafish embryos is boosted with high-speed, high-contrast imaging using the DM6 B microscope, ensuring accurate targeting for developmental…
Pancreatic Ductal Adenocarcinoma with 11 Apoptosis biomarkers shown – BAK, BAX, BCL2, BCLXL, Caspase9, CIAP1, NaKATPase, PCK26, SMAC, Vimentin, and XIAP.

Transforming Research with Spatial Proteomics Workflows

Spatial Proteomics, Nature Methods 2024 Method of the Year, is driving research advancements in cancer, immunology, and beyond. By combining positional data with high throughput imaging of proteins in…
C. elegans embedded in Lowicryl® HM20; pharynx showing red fluorescence (mCherry). The overview shows a front view onto the resin capsule formed by the bottom of a flow-through chamber of the EM AFS2. The capsule was pretrimmed manually. The blockface was trimmed automatically using the AutoTrim function of UC Enuity guided by fluorescence of the worm. Edge length of both squares in relation to the images is 250 µm.

How Fluorescence Guides Sectioning of Resin-embedded EM Samples

Electron microscopes, including transmission electron microscopes (TEM) and scanning electron microscopes (SEM), are widely utilized to gain detailed structural information about biological samples or…

Coherent Raman Scattering Microscopy Publication List

CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their…
Ivesta 3 with integrated monitor.

Präpariermikroskope

Wenn Sie Präparationen durchführen, verbringen Sie oft viele Stunden an den Okularen eines Präpariermikroskops. Bei Leica Microsystems können Sie aus einer Vielzahl von Mikroskopen und einem…
Large volume computational clearing processed Thunder image of human pancreatic islet organoid. Cells segmented using Segment By Example tool, automatically phenotyped, and color-coded based on phenotypes in Aivia. Image courtesy of the Matthias von Herrath Lab, La Jolla Institute of Immunology, La Jolla, CA.

Get to Insights Faster and Easier with AI Image Analysis Tools

Discover how Aivia helps scientists streamline image analysis with fast setup, accurate AI detection, and easy batch processing.
Image: Human stem cell-derived mid brain organoids. Courtesy of Dr Tanya Singh, University of Oxford.

Unlocking the Secrets of Organoid Models in Biomedical Research

Get ready to delve deeper into the world of organoids and 3D models, which are essential tools for advancing our understanding of human health. Navigating these complex structures and obtaining clear…
Spherulitic crystals of hippuric acid, which were imaged with a Leica microscope using crossed polarizers, showing so-called Maltese crosses.

A Guide to Polarized Light Microscopy

Polarized light microscopy (POL) enhances contrast in birefringent materials and is used in geology, biology, and materials science to study minerals, crystals, fibers, and plant cell walls.
Visoria B

Factors to Consider when Selecting Clinical Microscopes

What matters if you would like to purchase a clinical microscope? Learn how to arrive at the best buying decision from our Science Lab Article.
Histopathological sample, 40x magnification

Clinical Microscopy: Considerations on Camera Selection

The need for images in pathology laboratories has significantly increased over the past few years, be it in histopathology, cytology, hematology, clinical microbiology, or other applications. They…
C. elegans nematode embedded in Epon epoxy resin, contrasted with osmium tetroxide. The resin block was pretrimmed by hand. Scale bar: 500 µm.

How to Save Time and Samples by Automated Ultramicrotomy

This article describes how 3D micro-CT data of a resin-embedded electron microscopy sample can be used to trim the specimen down to a defined target plane prior to sectioning. The interactive and…
Transfection using the Uncommon Bio reprogramming system. Image acquired using the THUNDER Imager 3D Cell Culture with THUNDER Large Volume Computational Clearing (LVCC) applied. Image courtesy of Samuel East, Uncommon Bio.

Designing the Future with Novel and Scalable Stem Cell Culture

Visionary biotech start-up Uncommon Bio is tackling one of the world’s biggest health challenges: food sustainability. In this webinar, Stem Cell Scientist Samuel East shows how they make stem cell…
Multiplexed Cell DIVE imaging of Adult Human Alzheimer’s Brain Tissue labelled with 15 antibodies targeted towards markers specific to astrocytes (GFAP, S100B), microglia (TMEM119, IBA1), and Alzheimer’s-associated markers (β-amyloid and p-Tau217).

Explore Alzheimer's Spatial Proteome with Big Data

Alzheimer's disease, a genetic and sporadic neurodegenerative condition, leads to cognitive decline in mid to late life, marked by β-amyloid plaques and tau tangles. With limited treatment options,…
Documentation of an automotive clutch friction surface with a digital microscope

Automotive Part Verification and Development according to Specifications

Automotive part verification during the development and production of parts and components by suppliers or manufacturers is important for ensuring that specifications are met. Specifications are…
3D culture of ovarian cancer cells imaged using the confocal mode of Mica.

Mica: A Game-changer for Collaborative Research at Imperial College London

This interview highlights the transformative impact of Mica at Imperial College London. Scientists explain how Mica has been a game-changer, expanding research possibilities and facilitating…
Complete camera overview of EM grid recorded with 3 channels. Inserts displaying the positions, where superresolved 3D confocal images were recorded. 3D renderings of these positions are shown in the zoomed inserts. Fluorescence channels (nuclei by Hoechst, blue; mitochondria by MitoTracker Green, green; lipid Droplets by Bodipy and Crimson Beads, red). Width of a grid square is 90 ?m, width of a grid bar is 35 ?m. Samples kindly provided by Ievgeniia Zagoriy, Mahamid-Group, EMBL Heidelberg, Germany.

From Bench to Beam: A Complete Correlative Cryo Light Microscopy Workflow

In the webinar entitled "A Multimodal Vitreous Crusade, a Cryo Correlative Workflow from Bench to Beam" a team of experts discusses the exciting world of correlative workflows for structural biology…
Area of a printed circuit board (PCB) which was imaged with extended depth of field (EDOF) using digital microscopy.

Depth of Field in Microscope Images

For microscopy imaging, depth of field is an important parameter when needing sharp images of sample areas with structures having significant changes in depth. In practice, depth of field is…
Pancreatic Ductal Adenocarcinoma with 11 Aerobic Glycolysis/Warburg Effect biomarkers shown – BCAT, Glut1, HK2, HTR2B, LDHA, NaKATPase, PCAD, PCK26, PKM2, SMA1, and Vimentin.

Dive into Pancreatic Cancer Research with Big Data

Pancreatic cancer, with a mortality rate near 40%, is challenging to treat due to its proximity to major organs. This story explores the complex biology of pancreatic ductal adenocarcinoma (PDAC),…
Colon adenocarcinoma and normal colon at the tumor margin. 13 biomarkers shown including Cadherin, CD3, CD4, CD8, CD20, CD31, CD45, Collagen, Caspase 9, BCL2, Beta-Catenin, Vimentin, and Smooth Muscle Actin.

Uncover the Hidden Complexity of Colon Cancer with Big Data

Colorectal cancer poses a significant health burden. While surgery is effective initially, some patients develop recurrent secondary disease with poor prognosis, necessitating advanced therapies like…
Digital microscopy simplifies documenting cell-culture results electronically while following 21 CFR part 11 guidelines for biopharma.

Introduction to 21 CFR Part 11 for Electronic Records of Cell Culture

This article provides an introduction to the recommendations of 21 CFR Part 11 from the FDA, specifically focusing on the audit trail and user management in the context of cell-culture laboratories.…
Blood vessel system of a zebrafish larvae

Overcoming Challenges with Microscopy when Imaging Moving Zebrafish Larvae

Zebrafish is a valuable model organism with many beneficial traits. However, imaging a full organism poses challenges as it is not stationary. Here, this case study shows how zebrafish larvae can be…

Neurowissenschaften

Arbeiten Sie an einem besseren Verständnis neurodegenerativer Erkrankungen oder an einer Untersuchung der Funktionen des Nervensystems? Erfahren Sie, wie Sie mit Bildgebungslösungen von Leica…
Cell DIVE multiplexed image of FFPE tissue section from syngeneic murine cancer model, 4T1.

Mapping Tumor Immune Landscape with AI-Powered Spatial Proteomics

Spatial mapping of untreated tumors provides an overview of the tumor immune architecture, useful for understanding therapeutic responses. Immunocompetent murine models are essential for identifying…
Block-face created by automatic trimming under fluorescence. Mammalian cells of interest, stained with CellTrackerTM Green are visualized within the block-face using the UC Enuity equipped with the stereo microscope M205 FA. In the background a carbon finder grid in black is visible. All samples in the article are created by Felix Gaedke, PhD, CECAD, Cologne, Germany.

How to Automatically Obtain Fluorescent Cells of Interest in a Block-face

Block-face created by automatic trimming under fluorescence. Mammalian cells of interest, stained with CellTrackerTM Green are visualized within the block-face using the UC Enuity equipped with the…
Automated Laser Microdissection for Proteome Analysis

Deep Visual Proteomics Provides Precise Spatial Proteomic Information

Despite the availability of imaging methods and mass spectroscopy for spatial proteomics, a key challenge that remains is correlating images with single-cell resolution to protein-abundance…
These images show the microstructure of a hard metal with 10% cobalt which is used for heavy-duty tools. The large increase in magnification of the right image (compared to the left) has a risk of being outside the useful range or, in other words, empty magnification.

What is Empty Magnification and How can Users Avoid it

The phenomenon of “empty magnification”, which can occur while using an optical, light, or digital microscope, and how it can be avoided is explained in this article. The performance of an optical…
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