THUNDER Imager Live Cell & 3D Cell Culture & 3D Assay

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The Role of Iron Metabolism in Cancer Progression

Iron metabolism plays a role in cancer development and progression, and modulates the immune response. Understanding how iron influences cancer and the immune system can aid the development of new…
The Role of Iron Metabolism in Cancer Progression

How is Microscopy Used in Spatial Biology? A Microscopy Guide

Different spatial biology methods in microscopy, such as multiplex imaging, are helping to better understand tissue landscapes. Learn more in this microscopy guide.
How is Microscopy Used in Spatial Biology? A Microscopy Guide

Cell DIVE: Unraveling pathways in pancreatic cancer

Unlock new insights into tumour-immune interactions with spatial biology. Join our webinar to learn how to label >60 biomarkers per tissue section and use advanced clustering and analysis techniques…
Cell DIVE: Unraveling pathways in pancreatic cancer

Going Beyond Deconvolution

Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…
Going Beyond Deconvolution

Investigating Epithelial Morphogenesis and the Growth of Epithelia

This article shows how rapidly acquired THUNDER images of Drosophila follicles or egg chambers help distinguish apicobasal proteins when studying epithelial morphogenesis for cancer research.
Investigating Epithelial Morphogenesis and the Growth of Epithelia

Diseases Linked to Scaffold Proteins and Signaling

This article shows how diseases related to scaffold proteins and protein signaling can be studied in zebrafish models efficiently with a THUNDER Imager.
Diseases Linked to Scaffold Proteins and Signaling

Understanding Better the Onset of Diabetes

This article shows how Interleukin-17 (IL-17) proinflammatory cytokine proteins in human pancreatic islets can be studied efficiently with a THUNDER Imager.
Understanding Better the Onset of Diabetes

The Neural Crest (NC)

This article discusses how the study of neural crest (NC) development in chicken embryos is aided with haze-free imaging using a THUNDER Imager 3D Assay. Proper specification, migration, and…
The Neural Crest (NC)

Studying Ocular Birth Defects

This article discusses how lens formation and ocular birth defects can be studied with sharp widefield microscopy images which are acquired rapidly. The mouse ocular lens is used as a model to study…
Studying Ocular Birth Defects

Healthy Oocyte Development

This article discusses how the study of mechanisms involved in the generation of healthy oocytes in Drosophila fruit flies can be helped with sharp, haze-free images acquired with a THUNDER Imager…
Healthy Oocyte Development

Fast, High Acuity Imaging and AI-assisted Analysis

The use of state-of-the-art AI systems is pushing image analysis into a new generation. Challenges like the conflict between imaging power and sample integrity are being overcome with THUNDER’s…
Fast, High Acuity Imaging and AI-assisted Analysis

Create New Options for Live Cell Imaging

The use of state-of-the-art AI systems is pushing image analysis into a new generation. Challenges like the conflict between imaging power and sample integrity are being overcome with THUNDER’s…
Create New Options for Live Cell Imaging

Expression of D2-Dopamine Receptors in Neurons

This article discusses how D2-dopamine receptors (D2R) in the substantia-nigra of mouse brain can be more clearly revealed with a THUNDER Imager 3D Assay using Instant Computational Clearing (ICC)…
Expression of D2-Dopamine Receptors in Neurons

Investigating Pulmonary Vascular Diseases

This article describes how the cellular and molecular programs governing the formation and maintenance of lung blood vessels, as well as what goes wrong with them when pulmonary vascular diseases…
Investigating Pulmonary Vascular Diseases

Visualizing Retinal Interactions to Study Eye Diseases

This article shows how interactions between endothelial cells, blood vessels, microglia, and astrocytes in mouse retina can be studied efficiently with a THUNDER Imager 3D Cell Culture and Large…
Visualizing Retinal Interactions to Study Eye Diseases

Cranial Nerve Development

This article demonstrates how fast, high-contrast imaging of mouse embryos with a THUNDER Imager 3D Cell Culture and Large Volume Computational Clearing (LVCC) enable the investigation of axonal…
Cranial Nerve Development

Accurately Analyze Fluorescent Widefield Images

The specificity of fluorescence microscopy allows researchers to accurately observe and analyze biological processes and structures quickly and easily, even when using thick or large samples. However,…
Accurately Analyze Fluorescent Widefield Images

Save time and effort with AI-assisted fluorescence image analysis

The powerful synergy of THUNDER and Aivia analyze fluorescence images with greater accuracy, even when using low light excitation.
Save time and effort with AI-assisted fluorescence image analysis

Role of Mucins and Glycosylation in Dry Eye Disease

This article shows how fast, high-contrast, and sharp imaging of stratified human corneal epithelial cells with THUNDER imaging technology for dry eye disease (DED) research allows membrane ridges to…
Role of Mucins and Glycosylation in Dry Eye Disease

Skeletal Muscle Adaptation and Fibrotic Diseases

The mechanisms of how skeletal muscle adapts to fibrotic pathologies can be investigated more efficiently with fast, high-contrast imaging of C2C12 mouse myoblast cells which is described in this…
Skeletal Muscle Adaptation and Fibrotic Diseases

How to Improve Live Cell Imaging with Coral Life

For live-cell CLEM applications, light microscopy imaging is a critical step for identifying the right cell in the right state at the right time. In this article, Leica experts share their insights on…
How to Improve Live Cell Imaging with Coral Life

Optimizing THUNDER Platform for High-Content Slide Scanning

With rising demand for full-tissue imaging and the need for FL signal quantitation in diverse biological specimens, the limits on HC imaging technology are tested, while user trainability and…
Optimizing THUNDER Platform for High-Content Slide Scanning

Physiology Image Gallery

Physiology is about the processes and functions within a living organism. Research in physiology focuses on the activities and functions of an organism’s organs, tissues, or cells, including the…
Physiology Image Gallery

Monitoring Immunosuppressive Mechanisms from Infection

This application note discusses the importance of fast, sensitive, and haze-free imaging for the monitoring of immunosuppression in mouse lung epithelial tissue infected with the Puerto Rico 8…
Monitoring Immunosuppressive Mechanisms from Infection

Neuroscience Images

Neuroscience commonly uses microscopy to study the nervous system’s function and understand neurodegenerative diseases.
Neuroscience Images

Developmental Biology Image Gallery

Developmental biology explores the development of complex organisms from the embryo to adulthood to understand in detail the origins of disease. This category of the gallery shows images about…
Developmental Biology Image Gallery

“Brains-In-A-Dish” from Induced Pluripotent Stem Cells (iPSCs)

This article discusses the benefits of using the THUNDER technology for imaging inside 3D human cortical brain organoids. These organoids are derived from human induced pluripotent stem cells (iPSCs)…
“Brains-In-A-Dish” from Induced Pluripotent Stem Cells (iPSCs)

Developing Heart Pacemaker Cells from Cardiac Spheroids

During the last decade, 3D cell culture has been established as a more realistic model compared to classical 2D culture systems. Cells can develop into miniature 3D objects, so called spheroids, which…
Developing Heart Pacemaker Cells from Cardiac Spheroids

Putting Dynamic Live Cell Data into the Ultrastructural Context

With workflow Coral Life, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the right…
Putting Dynamic Live Cell Data into the Ultrastructural Context

Into the Third Dimension with "Wow Effect"- Observe Cells in 3D and Real-Time

Life is fast, especially for a cell. As a rule, cells should be examined under physiological conditions which are as close as possible to their natural environment. New technologies offer tremendous…
Into the Third Dimension with "Wow Effect"- Observe Cells in 3D and Real-Time

Observing 3D Cell Cultures During Development

3D cell cultures, such as organoids and spheroids, give insights into cells and their interactions with their microenvironment. These 3D cell cultures are playing an increasingly important role for…
Observing 3D Cell Cultures During Development

Cytosolic Calcium Ions in Melanoma Cancer Cells

In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell loading and fluorescence microscopy imaging is presented…
Cytosolic Calcium Ions in Melanoma Cancer Cells

Download The Guide to Live Cell Imaging

In life science research, live cell imaging is an indispensable tool to visualize cells in a state as in vivo as possible. This E-book reviews a wide range of important considerations to take to…
Download The Guide to Live Cell Imaging

Studying Cell Division

Cell division is a biological process during which all cellular components must be distributed among the daughter cells. The division process requires firm coordination for success. Microscopy is…
Studying Cell Division

Influenza-host Interactions in 3D Specimens

Stefan Finke studies virus-host interactions. It turns out that the ways the virus and host interact are different when comparing results from classical laboratory monolayer cell lines and “close to…
Influenza-host Interactions in 3D Specimens

Finding new Scaffolds for Tissue Engineering

Tissue engineers use biomaterials for a variety of applications from drug delivery to supporting the regeneration of damaged or lost tissues to creating in vitro disease models. Scaffold architecture…
Finding new Scaffolds for Tissue Engineering

The Power of Pairing Adaptive Deconvolution with Computational Clearing

Learn how deconvolution allows you to overcome losses in image resolution and contrast in widefield fluorescence microscopy due to the wave nature of light and the diffraction of light by optical…
The Power of Pairing Adaptive Deconvolution with Computational Clearing

Improvement of Imaging Techniques to Understand Organelle Membrane Cell Dynamics

Understanding cell functions in normal and tumorous tissue is a key factor in advancing research of potential treatment strategies and understanding why some treatments might fail. Single-cell…
Improvement of Imaging Techniques to Understand Organelle Membrane Cell Dynamics

Studying Autoimmune Disease

This article discusses how autoimmune diseases, like systemic lupus erythematosus (SLE), can be studied more efficiently using thick, 3D kidney tissue specimens visualized with a THUNDER Imager . SLE…
Studying Autoimmune Disease

Image Gallery: THUNDER Imager

To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve…
Image Gallery: THUNDER Imager

From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy

Obtaining high-quality data and images from thick 3D samples is challenging using traditional widefield microscopy because of the contribution of out-of-focus light. In this webinar, Falco Krüger…
From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy

Studying Human Brain Development and Disease

Neural spheroids created from human induced pluripotent stem cells (iPSCs) provide effective and novel tools for studying brain development, as well as the underlying pathological mechanisms of…
Studying Human Brain Development and Disease

An Introduction to Computational Clearing

Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…
An Introduction to Computational Clearing

Factors to Consider When Selecting a Research Microscope

An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
Factors to Consider When Selecting a Research Microscope

How Can Immunofluorescence Aid Virology Research?

Modern virology research has become as crucial now as ever before due to the global COVID-19 pandemic. There are many powerful technologies and assays that virologists can apply to their research into…
How Can Immunofluorescence Aid Virology Research?

Computational Clearing - Enhance 3D Specimen Imaging

This webinar is designed to clarify crucial specifications that contribute to THUNDER Imagers' transformative visualization of 3D samples and improvements within a researcher's imaging-related…
Computational Clearing - Enhance 3D Specimen Imaging

THUNDER Imagers: High Performance, Versatility and Ease-of-Use for your Everyday Imaging Workflows

This webinar will showcase the versatility and performance of THUNDER Imagers in many different life science applications: from counting nuclei in retina sections and RNA molecules in cancer tissue…
THUNDER Imagers: High Performance, Versatility and Ease-of-Use for your Everyday Imaging Workflows

Evaluating Axon Regeneration After Brain or Spine Trauma of Mice

Damaged nerve regeneration was investigated using mouse spinal cord sections treated with compounds that counter axon growth inhibitor (AGI) proteins. The sections were screened to find active and…
Evaluating Axon Regeneration After Brain or Spine Trauma of Mice

Drosophila Testis Niche Stem Cells – Three Color Computational Clearing

Differentiated living beings such as humans, but also a fruit fly or a plant, possess not only the differentiated cells which form specific tissues, but also those cells whose fate is not yet (or only…
Drosophila Testis Niche Stem Cells – Three Color Computational Clearing

Alzheimer Plaques: fast Visualization in Thick Sections

More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this…
Alzheimer Plaques: fast Visualization in Thick Sections

Real Time Images of 3D Specimens with Sharp Contrast Free of Haze

THUNDER Imagers deliver in real time images of 3D specimens with sharp contrast, free of the haze or out-of-focus blur typical of widefield systems. They can even image clearly places deep inside a…
Real Time Images of 3D Specimens with Sharp Contrast Free of Haze

Campos de aplicação

Imagens de células vivas

Alterando a perspectiva de componentes únicos de um microscópio a uma solução de imagens de células vivas completamente em funcionamento, a Leica Microsystems integra o microscópio, o software de…

Microscopia de fluorescência

A fluorescência é um dos fenômenos físicos mais comumente usados na microscopia biológica e analítica, principalmente devido a sua alta sensibilidade e alta especificidade. A fluorescência é uma forma…

Pesquisa sobre peixe-zebra

Para os melhores resultados durante a verificação, triagem, manipulação e aquisição de imagem, você precisa ver osdetalhes e estruturas que permitem tomar as decisões certas para os próximos passos em…

Fotomanipulação

O termo fotomanipulação engloba uma faixa de técnicas que utilizam as propriedades de moléculas fluorescentes para iniciar eventos e observar como complexos dinâmicos comportam-se com o passar do…

Neurocientífica

Você está trabalhando rumo a uma melhor compreensão de doenças neurodegenerativas ou estudando a função do sistema nervoso? Veja como você pode fazer descobertas com as soluções de aquisição de…

Organoides e cultura celular 3D

Um dos recentes avanços mais impressionantes na pesquisa de ciências da vida é o desenvolvimento de sistemas de cultura celular 3D, como organoides, esferoides ou modelos órgão-em-chip. Uma cultura…

Virology

Soluções para aquisição de imagens e preparação de amostras para pesquisas em virologia

Técnicas avançadas de microscopia

As técnicas avançadas de microscopia abrangem tanto técnicas de imagem de alta resolução quanto de super-resolução. Estas técnicas são usadas principalmente para visualizar eventos biológicos em…

Técnicas básicas de microscopia

Técnicas básicas de microscopia são usadas onde todo o espécime no estágio de microscopia será exposto a uma fonte de luz. A amostra inteira é iluminada por luz branca tanto por cima (em uma…

Microscópios DIC

Um microscópio DIC oferece microscopia de campo amplo e tem um filtro de polarização e prisma Wollaston entre a fonte de luz e a lente do condensador e também entre a lente objetiva e o sensor da…

Microscópios de campo escuro

O método de contraste de campo escuro explora a difração ou a dispersão de luz a partir de estruturas de uma amostra biológica ou as características não uniformes de uma amostra de material.
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