Decodifique a biologia 3D em tempo real*
THUNDER Imager Live Cell & 3D Cell Culture & 3D Assay
Pesquisa em engenharia química farmacêutica
Seu papel na fabricação, pesquisa e desenvolvimento de produtos farmacêuticos e químicos exige soluções de microscópios, câmeras e software para ajudá-lo a visualizar, analisar e documentar de forma…
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![[Translate to portuguese:]](https://webcdn.leica-microsystems.com/fileadmin/_processed_/1/7/csm_lms-life-science-billboard-subpages-header_live-cell-imaging-01_6cbc67e77d.jpg "Imagens de células vivas")
Imagens de células vivas
Alterando a perspectiva de componentes únicos de um microscópio a uma solução de imagens de células vivas completamente em funcionamento, a Leica Microsystems integra o microscópio, o software de…
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![[Translate to portuguese:]](https://webcdn.leica-microsystems.com/fileadmin/_processed_/5/e/csm_lms-life-science-fluorescence-header_d87141576b.jpg "Microscopia de fluorescência")
Microscopia de fluorescência
A fluorescência é um dos fenômenos físicos mais comumente usados na microscopia biológica e analítica, principalmente devido a sua alta sensibilidade e alta especificidade. A fluorescência é uma forma…
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![[Translate to portuguese:]](https://webcdn.leica-microsystems.com/fileadmin/_processed_/b/5/csm_lms-life-science-billboard-subpages-header_zebrafish-02_a1b512b366.jpg "Pesquisa sobre peixe-zebra")
Pesquisa sobre peixe-zebra
Para os melhores resultados durante a verificação, triagem, manipulação e aquisição de imagem, você precisa ver osdetalhes e estruturas que permitem tomar as decisões certas para os próximos passos em…
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Fotomanipulação
O termo fotomanipulação engloba uma faixa de técnicas que utilizam as propriedades de moléculas fluorescentes para iniciar eventos e observar como complexos dinâmicos comportam-se com o passar do…
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Neurocientífica
Você está trabalhando rumo a uma melhor compreensão de doenças neurodegenerativas ou estudando a função do sistema nervoso? Veja como você pode fazer descobertas com as soluções de aquisição de…
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Organoides e cultura celular 3D
Um dos recentes avanços mais impressionantes na pesquisa de ciências da vida é o desenvolvimento de sistemas de cultura celular 3D, como organoides, esferoides ou modelos órgão-em-chip. Uma cultura…
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Virology
Soluções para aquisição de imagens e preparação de amostras para pesquisas em virologia
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Science Lab Topic:
Fluorescence Microscopy
Role of Mucins and Glycosylation in Dry Eye Disease
This article shows how fast, high-contrast, and sharp imaging of stratified human corneal epithelial cells with THUNDER imaging technology for dry eye disease (DED) research allows membrane ridges to…
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Science Lab Topic:
Fluorescence Microscopy
Skeletal Muscle Adaptation and Fibrotic Diseases
The mechanisms of how skeletal muscle adapts to fibrotic pathologies can be investigated more efficiently with fast, high-contrast imaging of C2C12 mouse myoblast cells which is described in this…
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Science Lab Topic:
CLEM
How to Improve Live Cell Imaging with Leica Nano Workflow
For live-cell CLEM applications, light microscopy imaging is a critical step for identifying the right cell in the right state at the right time. In this article, Leica experts share their insights on…
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Science Lab Topic:
Fluorescence Microscopy
Optimizing THUNDER Platform for High-Content Slide Scanning
With rising demand for full-tissue imaging and the need for FL signal quantitation in diverse biological specimens, the limits on HC imaging technology are tested, while user trainability and…
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Science Lab Topic:
Fluorescence Microscopy
Physiology Image Gallery
Physiology is about the processes and functions within a living organism. Research in physiology focuses on the activities and functions of an organism’s organs, tissues, or cells, including the…
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Science Lab Topic:
Fluorescence Microscopy
Monitoring Immunosuppressive Mechanisms from Infection
This application note discusses the importance of fast, sensitive, and haze-free imaging for the monitoring of immunosuppression in mouse lung epithelial tissue infected with the Puerto Rico 8…
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Science Lab Topic:
Neuroscience
Neuroscience Image Gallery
Neuroscience commonly uses microscopy to study the nervous system’s function and understand neurodegenerative diseases.
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Science Lab Topic:
Fluorescence Microscopy
Developmental Biology Image Gallery
Developmental biology explores the development of complex organisms from the embryo to adulthood to understand in detail the origins of disease. This category of the gallery shows images about…
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Science Lab Topic:
Fluorescence Microscopy
“Brains-In-A-Dish” from Induced Pluripotent Stem Cells (iPSCs)
This article discusses the benefits of using the THUNDER technology for imaging inside 3D human cortical brain organoids. These organoids are derived from human induced pluripotent stem cells (iPSCs)…
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Science Lab Topic:
Widefield Microscopy
Developing Heart Pacemaker Cells from Cardiac Spheroids
During the last decade, 3D cell culture has been established as a more realistic model compared to classical 2D culture systems. Cells can develop into miniature 3D objects, so called spheroids, which…
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Science Lab Topic:
EM Sample Preparation
Putting Dynamic Live Cell Data into the Ultrastructural Context
With the Leica Nano Workflow, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the…
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Science Lab Topic:
Widefield Microscopy
Into the Third Dimension with "Wow Effect"- Observe Cells in 3D and Real-Time
Life is fast, especially for a cell. As a rule, cells should be examined under physiological conditions which are as close as possible to their natural environment. New technologies offer tremendous…
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Science Lab Topic:
Widefield Microscopy
Observing 3D Cell Cultures During Development
3D cell cultures, such as organoids and spheroids, give insights into cells and their interactions with their microenvironment. These 3D cell cultures are playing an increasingly important role for…
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Science Lab Topic:
Widefield Microscopy
Cytosolic Calcium Ions in Melanoma Cancer Cells
In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell loading and fluorescence microscopy imaging is presented…
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Science Lab Topic:
Widefield Microscopy
Download The Guide to Live Cell Imaging
In life science research, live cell imaging is an indispensable tool to visualize cells in a state as in vivo as possible. This E-book reviews a wide range of important considerations to take to…
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Science Lab Topic:
Fluorescence Microscopy
Studying Cell Division
Cell division is a biological process during which all cellular components must be distributed among the daughter cells. The division process requires firm coordination for success. Microscopy is…
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Science Lab Topic:
Fluorescence Microscopy
Viral Infections – Studying Influenza-host Interactions in 3D Specimens
Stefan Finke studies virus-host interactions. It turns out that the ways the virus and host interact are different when comparing results from classical laboratory monolayer cell lines and “close to…
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Science Lab Topic:
Fluorescence Microscopy
Finding new Scaffolds for Tissue Engineering
Tissue engineers use biomaterials for a variety of applications from drug delivery to supporting the regeneration of damaged or lost tissues to creating in vitro disease models. Scaffold architecture…
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Science Lab Topic:
Widefield Microscopy
The Power of Pairing Adaptive Deconvolution with Computational Clearing
Deconvolution is a computational method used to restore the image of the object that is corrupted by the point spread function (PSF) along with sources of noise. In this technical brief, learn how the…
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Science Lab Topic:
Fluorescence Microscopy
Improvement of Imaging Techniques to Understand Organelle Membrane Cell Dynamics
Understanding cell functions in normal and tumorous tissue is a key factor in advancing research of potential treatment strategies and understanding why some treatments might fail. Single-cell…
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Science Lab Topic:
Fluorescence Microscopy
Studying Autoimmune Disease
This article discusses how autoimmune diseases, like systemic lupus erythematosus (SLE), can be studied more efficiently using thick, 3D kidney tissue specimens visualized with a THUNDER Imager . SLE…
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Science Lab Topic:
Fluorescence Microscopy
Image Gallery: THUNDER Imager
To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve…
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Science Lab Topic:
Fluorescence Microscopy
From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy
Obtaining high-quality data and images from thick 3D samples is challenging using traditional widefield microscopy because of the contribution of out-of-focus light. In this webinar, Falco Krüger…
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Science Lab Topic:
Widefield Microscopy
Studying Human Brain Development and Disease
Neural spheroids created from human induced pluripotent stem cells (iPSCs) provide effective and novel tools for studying brain development, as well as the underlying pathological mechanisms of…
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Science Lab Topic:
Widefield Microscopy
An Introduction to Computational Clearing
Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…
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Science Lab Topic:
Basics in Microscopy
Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
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Science Lab Topic:
Confocal Microscopy
How Can Immunofluorescence Aid Virology Research?
Modern virology research has become as crucial now as ever before due to the global COVID-19 pandemic. There are many powerful technologies and assays that virologists can apply to their research into…
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Science Lab Topic:
Widefield Microscopy
Studying Natural Killer (NK) Cells Derived from Induced Pluripotent Stem Cells (iPSC)
The study of natural killer (NK) cells holds tremendous promise for developing novel immunotherapies. NK cells derived from induced pluripotent stem cells (iPSCs) can be used to create an easily…
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Science Lab Topic:
Basics in Microscopy
Getting Sharper 3D Images of Thick Biological Specimens with Widefield Microscopy
Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…
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Science Lab Topic:
Fluorescence Microscopy
IL-18 cytokine derived from the enteric nervous system is important for intestinal immunity
Interleukin 18 (IL-18) is a proinflammatory cytokine, which induces cell-mediated immune reaction upon bacterial infection. In the intestine, it is known that IL-18 is produced in immune and…
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Science Lab Topic:
Fluorescence Microscopy
Cholesterol Homeostasis Modulates Platinum Sensitivity in Human Ovarian Cancer
Ovarian cancer is one of the most severe types of cancers that women can suffer from during their lifetimes. It is the cancer’s tendency for frequent relapses and drug resistance that leads to…
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Science Lab Topic:
Widefield Microscopy
Computational Clearing - Enhance 3D Specimen Imaging
This webinar is designed to clarify crucial specifications that contribute to THUNDER Imagers' transformative visualization of 3D samples and improvements within a researcher's imaging-related…
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Science Lab Topic:
Widefield Microscopy
THUNDER Imagers: High Performance, Versatility and Ease-of-Use for your Everyday Imaging Workflows
This webinar will showcase the versatility and performance of THUNDER Imagers in many different life science applications: from counting nuclei in retina sections and RNA molecules in cancer tissue…
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Science Lab Topic:
Widefield Microscopy
Evaluating Axon Regeneration After Brain or Spine Trauma of Mice
Damaged nerve regeneration was investigated using mouse spinal cord sections treated with compounds that counter axon growth inhibitor (AGI) proteins. The sections were screened to find active and…
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Science Lab Topic:
Fluorescence Microscopy
Interview with Magali Mondin on THUNDER Imagers
Magali Mondin, an engineer at the BIC (Bordeaux Imaging Center) in France, describes her experience using a THUNDER Imager in this interview. She had the opportunity to test extensively the THUNDER…
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Science Lab Topic:
Widefield Microscopy
Drosophila Testis Niche Stem Cells – Three Color Computational Clearing
Differentiated living beings such as humans, but also a fruit fly or a plant, possess not only the differentiated cells which form specific tissues, but also those cells whose fate is not yet (or only…
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Science Lab Topic:
Widefield Microscopy
Alzheimer Plaques: fast Visualization in Thick Sections
More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this…
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Science Lab Topic:
Widefield Microscopy
THUNDER Technology Note
So far, widefield microscopy was not suitable to image larger volumes, since the contrast of the recorded fluorescence image is reduced by the background (BG) mainly originating from out of focus…
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